Scientific Reports
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2024
·Vol. 14
·期 1
·1-12
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IF 3.8
Wang Tao,Zheng Qian,Yang Qian,Guo Fang,Cui Haiyan,Hu Meng,Zhang Chao,Chen Zhe,Fu Shanlin,Guo Zhongyuan,Wei Zhiwen,Yun Keming
Ketamine has recently become an anesthetic drug used in human and veterinary clinical medicine for illicit abuse worldwide, but the detection of illicit abuse and inference of time intervals following ketamine abuse are challenging issues in forensic toxicological investigations. Here, we developed methods to estimate time intervals since ketamine use is based on significant metabolite changes in rat serum over time after a single intraperitoneal injection of ketamine, and global metabolomics was quantified by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF–MS). Thirty-five rats were treated with saline (control) or ketamine at 3 doses (30, 60, and 90 mg/kg), and the serum was collected at 21 time points (0 h to 29 d). Time-dependent rather than dose-dependent features were observed. Thirty-nine potential biomarkers were identified, including ketamine and its metabolites, lipids, serotonin and other molecules, which were used for building a random forest model to estimate time intervals up to 29 days after ketamine treatment. The accuracy of the model was 85.37% in the cross-validation set and 58.33% in the validation set. This study provides further understanding of the time-dependent changes in metabolites induced by ketamine abuse.
研究领域:
毒理学
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分析化学
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机器学习
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2025
·
IF 4.1
Xiaofeng Wang,Xiao Liu,Lixue Wu,Gang Hu,Zhaohui Fang
Chiral enantiomers of drugs often exhibit distinct pharmacological activities despite their similar physical and chemical properties. Developing efficient methods for their distinguishment is crucial for pharmaceutical quality control. Here, we presented a novel approach using a CTT (Chlorite-Tetramethylammonium Hydroxide-Tetrathionate) clock reaction system for kinetic chiral enantiomer recognition. DOPA enantiomers were selected for this purpose, in which L-DOPA is therapeutic efficacy in treating Parkinson's disease by precursoring dopamine while D-DOPA lacks such a function. By introducing equal amounts of L-DOPA and D-DOPA into the CTT clock system, we observed a more prolonged induction period for L-DOPA compared to D-DOPA, enabling their quick differentiation. Moreover, a linear relationship between the induction period and the concentration (1.190 × 10 −6 mol/L to 4.5238 × 10 −6 mol/L) of L-DOPA or D-DOPA allowed quantitative detection of L-DOPA or D-DOPA samples. This method could also be applied to analyze varying concentration ratios of L-DOPA and D-DOPA mixtures at a fixed total concentration. Study on mechanism indicates that both L-DOPA and D-DOPA react with ClO 2 · radical to finally generate DHICA through oxidation, with their kinetic disparities evident during this process. These kinetic disparities between L-DOPA and D-DOPA oxidation arises from stereospecific hydrogen-bonding interactions in the cyclodopa intermediate. The weaker hydrogen bond in L-DOPA-derived cyclodopa likely facilitates its cleavage during oxidation, thereby accounting for the faster reaction kinetics observed in the clock system compared to D-DOPA. This makes L-DOPA exhibit more fast consumption of chlorine dioxide than D-DOPA, and resulting larger the extension in the induction period with L-DOPA than that with D-DOPA. Such a method coupling CCT clock demonstrates its versatility and potential for broader applications in chiral drug analysis.
研究领域:
分析化学
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手性药物分析
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药学
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化学动力学
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生物化学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2021
·
IF 3.171
Jianhui Li,Yi Wang,Yang Chen,Qizhao Xiong,Jiangfeng Yang,Libo Li,Jinping Li
Harvesting water from the atmosphere is an important process to solve the extreme lack of water resources in arid regions. Adsorption-based atmospheric water harvesting (AWH) takes advantage of solar thermal energy to harvest water from air. This technique is particularly suitable for arid regions characterized by low humidity and an abundance of sunshine. Nonetheless, under low humidity conditions, AWH is highly dependent on water-adsorbing materials exhibiting excellent performance. In this work, a metal–organic framework (MOF), namely [Zn 2 (bpy)(btec)(H 2 O) 2 ]·2H 2 O, also denoted as MWH-1, was investigated for application in water harvesting under low humidity conditions (<20%). Notably, MWH-1 displayed outstanding water and thermal stability. At temperatures of 293–333 K and low pressure, activated MWH-1a exhibited competitive water uptake (relative humidity (RH) = 5%, uptake>200 cm 3 ·cm −3 ; RH = 10%, uptake>250 cm 3 ·cm −3 ). This ensured effective water harvesting at high temperatures during the day. In situ powder X-ray diffraction and Fourier-transform infrared analyses confirmed the sensitive water adsorption process of MWH-1a. The X-ray single-crystal study further demonstrated that single-crystal structures could be completely restored following water harvesting. MWH-1 showed good structural stability and enabled water harvesting under low humidity and high temperature conditions. Thus, it has the potential for application in round-the-clock water harvesting in extremely arid regions.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
Advanced Science
·
2026
·
IF 14.1
Xingwei Wang,Yufeng Xu,Yanfei Hu,Lijun Cao,Ru Jiang,Changtian Chen,Rick Masonbrink,Thomas Maier,Yuchen Tu,Yabo Shi,Enhui Liu,Lingan Kong,Chan Guo,Wei Zhao,Peng Shi,Wenzhen Du,Andrew Severin,Thomas Baum,Deliang Peng,Huan Peng,Mian Zhou,Wei Wang
The majority of commercially cultivated soybean cyst nematode (SCN)-resistant soybean cultivars rely on the Rhg1 locus to defend against SCN, the most economically destructive soybean root pathogen. The decline of Rhg1's effectiveness due to its continuous use creates an urgent need for alternative genetic sources to sustain SCN resistance. Although pathogen effectors are known to perturb host circadian clocks in other plant-pathogen systems, whether and how plant-parasitic nematodes interfere with the host clock to promote virulence remains unexplored. Here, we report that the soybean circadian clock gates rhythmic defense against SCN, and its core component, GmCCA1, plays a dual role in both circadian regulation and pathogen defense. Surprisingly, overexpression of GmCCA1 disrupts circadian rhythms but confers sustained resistance against SCN by activating defense genes both within and beyond the Rhg1 locus, including various pathogenesis-related and resistance genes. On the pathogen side, we identify Hg4E02—an evolutionarily conserved effector in plant-parasitic nematodes—as a transcriptional regulator that directly binds to the promoters of multiple clock genes via novel cis-elements, suppressing their expression. Hg4E02 also represses defense gene expression to enhance virulence. Notably, we uncover a previously unknown layer of antagonism: GmCCA1 and Hg4E02 exert opposing effects on both defense genes and translation-related genes. GmCCA1 inhibits translation and root growth, whereas Hg4E02 promotes translation, likely facilitating nutrient acquisition for the nematode. Collectively, our study reveals a novel mechanism by which a nematode effector directly targets the host circadian clock and translation machinery to promote parasitism, and proposes GmCCA1 as a promising engineering target for enhancing SCN resistance in soybean.
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Ruihua Zhang,Yuhan Gong,Maoyuan Yin,Xujun Chen,Longgang Ma,Zhiqiang Zhu,Xinqing Xiao
This work demonstrates a round-the-clock self-powered flexible spectral sensing device(FSSD) enabling in-situ fruit quality monitoring by resolving orchard energy supply limitations through synergistic photovoltaic power and moist-electric generation. This system integrates flexible solar patch (FSP) and flexible moisture-electric generator (FMEG) for hybrid energy harvesting, ensuring continuous operation through the compensation of photovoltaic power by moist-electric generation. The FMEG fabricated using the LiCl concentration-gradient design strategy to construct an ionic hydrogel film in a sandwich configuration which delivers the short-circuit current (Isc) of 0.73 V and short-circuit current density of 67.89 mA cm −2 at 80 % relative humidity. The FSP demonstrates excellent linear current response to illumination intensity from 5 k to 30 k lux, with short-circuit current (Isc) increasing from 12.81 mA to 115.21 mA, while delivering a stabilized Voc of 1.75 V at 30 k lux. The FSSD, which integrates spectral sensors and low-power control chip via flexible electronics technology and is powered by the hybrid energy supply, enables in-situ collection and wireless transmission of spectral data. Furthermore, through embedded partial least squares regression (PLSR) and support vector machine (SVM) machine learning models, it can realize real-time and continuous prediction of fruit quality parameters and evaluation of their ripeness levels. This easily manufacturable, scalable, and cost-effective system integrates autonomous energy harvesting with quality monitoring to construct a closed-loop digital decision-making framework for preharvest monitoring and post-harvest grading, which enables precision harvesting, reduces post-harvest losses, and advances technologies for precision agriculture and agricultural product traceability.
研究领域:
传感器与传感技术
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柔性电子学
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能量采集
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精准农业
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食品质量监测
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机器学习应用
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光谱学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2017
·
IF 7.504
Lei Zheng,Haili Yu,Yaling Yue,Fengjuan Wu,Yi He
We report a visual chronometric assay for Cr3+ based on the Cu2O nanocube-mediated clock reaction of methylene blue (MB) and N2H4. MB with a blue color can be easily reduced by excess N2H4 to form colorless leuco-MB in the presence of Cu2O nanocubes as a catalyst in a short time. However, the addition of Cr3+ decreases the catalytic activity of Cu2O nanocubes owing to the coordination interactions between them, leading to an increase in the reaction time. The reaction time change was employed for selective detection of Cr3+. This assay has a wide dynamic range from 0.03 to 600 μM with a limit of detection of 2.7 nM. Remarkably, different concentrations of Cr3+ can be directly detected with the naked eye by observing the color-fading speed. Additionally, this assay was successfully used to determine Cr3+ in real water samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
·
IF 4.9
Chunxiao Zhang,Hongxiao Yu,Yuanzhi Zhang,Kun Wang,Mengying Chen,Xu Wang
Inspired by the natural world's fascinating capacity for color change, researchers have developed various responsive hydrogels that shift colors. Among these, hydrogels that react to pH changes are particularly notable for their unique properties. The urea-urease autocatalytic reaction, a key pH clock reaction, is especially effective in temporarily adjusting these hydrogels' properties and enabling color changes in response to stimuli. In this study, we synergize the urea-urease clock reaction with the pH-sensitive color transformation of neutral red (NR), thereby establishing an NR-urea-urease clock reaction system. This system exhibits a dynamic color cycle from yellow to red and back to yellow. By incorporating this color transition mechanism into polymer hydrogels, we successfully initiate a pH change within the system. This innovation paves the way for a smart material capable of displaying temporary information through enzymatic reactions . The temporal variation in information display is finely tuned by adjusting the urea and urease concentrations. Such reversible color-changing hydrogels hold promising prospects for applications in secure information transmission and dynamic camouflage, marking a significant step forward in systems chemistry and materials science.
研究领域:
材料科学
·
系统化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
RENEWABLE ENERGY
·
2026
·
IF 9.1
Xuemin GENG,Jinghao ZHANG,Xiangrui MENG,Yan ZHAO,Siyu DENG,Peng YANG,Yanfen WAN
Freshwater scarcity and water quality issues pose significant constraints on societal development and progress. The critical tasks of water collection and treatment are of utmost importance. However, an integrated device that combines both functions has not garnered substantial interest. Herein, we have crafted a multifunctional, integrated device that concurrently addresses the challenges of water collection and purification. The device comprises a water collection module and an integrated interface solar water evaporator. The water collection system is built with a multifunctional hygroscopic aerogel felt and a specialized collection unit featuring an innovative groove pattern. The solar-driven water evaporation component comprises aerogel felt embedded with a FeCo 2 O 4 /polypyrrole composite photothermal material, as well as a biomimetic, root-like water conveyance device designed for efficient water transport. The device accomplishes a broad-spectrum high light absorption, robust water transport capability, and precise thermal energy management through the optimized light-absorber, the architectural design of evaporator and the engineering of water conveyance network. The system exhibited an impressive water absorption rate of 4.76 g g -1 , along with a substantial water evaporation rate of 3.54 kg m -2 h -1 under one Sun illumination. In the outdoor experiment with an average irradiation intensity of about 0.5 Sun , 1 m 2 can produce 6.5 L of water in 6 hours, which is fully enough for a family of three to meet the daily drinking water needs, thereby addressing water scarcity issues and supporting the execution of sustainable development initiatives.
✓ 已验证
DOI
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY A
·
2019
·
IF 2.641
György Csekö,Qingyu Gao,Changwei Pan,Li Xu,Attila K. Horváth
The thiourea dioxide–periodate reaction has been investigated under acidic conditions using phosphate buffer within the pH range of 1.1–2.0 at 1.0 M ionic strength adjusted by sodium perchlorate. Absorbance–time series are monitored as a function of time at 468 nm, the isosbestic point of the I2–I3– system. The profile of these kinetic runs follows either sigmoidal-shaped or rise-and-fall traces depending on the initial concentration ratio of the reactants. The clock species iodine appears after a well-defined but reproducible time lag even in substrate excess, meaning that the system may be classified as an autocatalysis-driven clock reaction. It is also demonstrated that the age of the thiourea dioxide solution markedly shortens the Landolt time, suggesting that the original form of thiourea dioxide (TDO) rearranges into a more reactive form and reacts faster than the original one. The behavior found is consistent with that recently observed in other oxidation reactions of TDO. To characterize the system quantitatively, a 22-step kinetic model is constructed from adapting the kinetic model of the TDO–iodate reaction published recently by supplementing it with six different reactions of periodate. By the help of seven fitted rate coefficients a sound agreement between the measured and calculated absorbance–time traces is obtained.
研究领域:
物理化学
·
反应动力学
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无机化学
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分析化学
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化学动力学
✓ 已验证
DOI
高影响因子
Theranostics
·
2019
·
IF 8.063
Shuai Wang,Yanke Lin,Ziyue Zhou,Lu Gao,Zemin Yang,Feng Li,Baojian Wu
Controlling bilirubin to a low level is necessary in physiology because of its severe neurotoxicity. Therefore, it is of great interest to understand the regulatory mechanisms for bilirubin homeostasis. In this study, we uncover a critical role for circadian clock in regulation of bilirubin detoxification and homeostasis. Methods : The mRNA and protein levels of Bmal1 (a core clock gene), metabolic enzymes and transporters were measured by qPCR and Western blotting, respectively. Luciferase reporter, mobility shift and chromatin immunoprecipitation were used to investigate transcriptional gene regulation. Experimental hyperbilirubinemia was induced by injection of bilirubin or phenylhydrazine. Unconjugated bilirubin (UCB) and conjugated bilirubin were assessed by ELISA. Results : We first demonstrated diurnal variations in plasma UCB levels and in main bilirubin-detoxifying genes Ugt1a1 and Mrp2 . Of note, the circadian UCB levels were antiphase to the circadian expressions of Ugt1a1 and Mrp2. Bmal1 ablation abrogated the circadian rhythms of UCB and bilirubin-induced hepatotoxicity in mice. Bmal1 ablation also decreased mRNA and protein expressions of both Ugt1a1 and Mrp2 in mouse livers, and blunted their circadian rhythms. A combination of luciferase reporter, mobility shift, and chromatin immunoprecipitation assays revealed that Bmal1 trans-activated Ugt1a1 and Mrp2 through specific binding to the E-boxes in the promoter region. Further, Bmal1 ablation caused a loss of circadian time-dependency in bilirubin clearance and sensitized mice to chemical induced-hyperbilirubinemia. Moreover, bilirubin stimulated Bmal1 expression through antagonism of Rev-erbα, constituting a feedback mechanism in bilirubin detoxification. Conclusion : These data supported a dual role for circadian clock in regulation of bilirubin detoxification, generating circadian variations in bilirubin level via direct transactivation of detoxifying genes Ugt1a1 and Mrp2, and defending the body against hyperbilirubinemia via Rev-erbα antagonism. Thereby, our study provided a potential mechanism for management of bilirubin related diseases.
研究领域:
分子生物学
·
昼夜节律
·
肝病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Energy
·
2024
·
IF 16.8
Kaiyang Wang,Shuhui Ren,Yunfang Jia,Xiaobing Yan
Central and peripheral biological clocks are essential to regulate creatures’ circadian rhythms, mimicking the biological clock controlled neural and/or immunological responses are research hotspots in the emerging neuromorphic or brain-like systems. However, the current neuromorphic devices still depend on programmable frequency dividing and doubling machine clocks, the high system cost is a challenge to conduct sophisticated brain-like neuromorphic computing. Herein, a dual biological-clocks (including ambient light and endocrine) controllable fibrous synapse crossbar array is proposed. It is demonstrated and confirmed in this work, the Ag conductive filaments, which are critical in the conventional MXene synapses, can be successfully switched by differently biased MoS 2 /MXene heterojunction. The current and power of the unit fibrous synapse in positive/negative switching processes can reach as low as 460 pA/-170 pA and 330 pW/121 pW, respectively. Then, four kinds of logical calculations to the input light signals are accomplished by using the as-prepared fibrous synapse based logical gate. Meanwhile, the light and serotonin combined modulation on the synaptic currents are manifested and applied to simulate the immune-neural responses (memory and the memory exhausted) of T cells in the skin clock. More interestingly, two biological immune compensation mechanisms controlled by the central clock and peripheral clock, namely balanced and reset immune compensation, are also accomplished. This work may pave a new way for promoting the development of dual biological-clock regulated computations in neuromorphic systems.
研究领域:
类脑工程
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生物钟
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材料科学
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神经科学
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免疫学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Fenggui He,Abuduaini Abulimiti,Boyuan Li,Yi Wu,Yi Chen,Minghui Zhang,Abdukader Abdukayum
Round-the-clock photocatalysts based on persistent luminescent nanoparticles (PLNPs) have great potential for water treatment and energy conservation, with continuous photocatalytic activity even at night or under dark conditions. However, their round-the-clock photocatalytic efficiency is still not high enough owing to the short persistent luminescence time, the low specific surface area, and the fast recombination rate of the light-generated e–/h+ pairs. Furthermore, no PLNP combinations with metal–organic frameworks (MOFs) for photocatalytic application have been reported as of yet. Here, we report a round-the-clock composite photocatalyst based on Zn3Ga2Ge2O10:0.5% Mn combined with NH2-MIL-101(Fe) via covalent bonding for the photo-degradation of RhB and the photo-reduction of Cr(VI). The PLNPs/MOFs composite photocatalyst exhibits high photocatalytic activity in the dark for more than 12 h due to the long afterglow time of more than 16 days of Zn3Ga2Ge2O10/0.5% Mn, the efficient persistent luminescence resonance energy transfer between PLNPs and MOFs, the large specific surface area of MOFs, the large number of active transition metal sites of MOFs, and the decrease of the photogenerated e–/h+ pair binding rate. The combination of PLNPs/MOFs provides a strategy for developing highly efficient round-the-clock photocatalysts, which offer great potential in environmental protection and utilization of solar energy.
研究领域:
环境科学
·
材料科学
·
化学
·
可持续能源
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）