INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaohan Zheng,Bowen Zou,Wenqiang Cai,Xianbing Xu,Ming Du,Xiaokang Na,Beiwei Zhu,Chao Wu
Cod proteins (CPs) have potential applications in designing desirable gel-based products, and this study aimed to unravel their heat-induced aggregation pattern and further probe the roles in protein gels. SDS-PAGE analysis indicated that high-precipitation-coefficient aggregates (HPCAs) of CPs aggregates were composed of considerable polymers of myosin heavy chains and actin, and their low-precipitation-coefficient aggregates (LPCAs) contained myosin light chains and tropomyosin . Studies from correlation analysis between the structure and aggregation kinetics revealed that the generation of β-sheet and S S bonds were responsible for their spontaneous thermal aggregation induced by heating temperature and protein concentration, respectively. Additionally, as protein denaturation ratio increased, more and larger HPCAs were formed, which was evidenced driving the network formation of protein gels and resulting in higher storage modulus (G') values. These novel findings may be applicable to other animal proteins for better tailoring the manufacturing of muscle gel-based products.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiaohan Zheng,Bowen Zou,Chao Ren,Xianbing Xu,Ming Du,Chao Wu
Cod proteins (CPs) are considered potential functional ingredients for developing gel-based foods, but present studies on the aggregation behavior of CPs upon heating remain limited. With this respect, the heat-induced aggregation kinetics of CPs at a subunit level was investigated. Based on different centrifugal forces, CPs aggregates were divided into three fractions: large-sized, intermediary-sized, and small-sized aggregates. SDS-PAGE and diagonal SDS-PAGE indicated that myosin heavy chains exhibited a higher affinity with actin to form intermediary-sized and large-sized aggregates; tropomyosin and myosin light chains were hardly engaged in the thermal aggregation and formed small-sized aggregates. The highly-polymerized aggregates adopted considerable transitions of helix-to-sheet in protein structures, whereas the structure of small-sized aggregates featured substantial helix-coil transitions. Furthermore, molecular interactions at different heating stages were revealed. These novel insights might advance our knowledge on the heat-induced aggregation behavior of CPs and provide fundamental information for the application of CPs in gel-based foods.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2020
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IF 6.551
Zhicheng Lang,Minghua Zhou,Qizhan Zhang,Xiaoya Yin,Yawei Li
The effective treatment of marine aquaculture wastewater is of great significance to protect marine environment and marine organisms. This study validated the feasibility of the comprehensive removal of NH 4 + -N, NO 2 − -N, COD and P, as well as disinfection and antibiotics removal from marine aquaculture wastewater by electrochemical oxidation (EO), comparing the performance and energy consumption with that by electro-peroxone (EP) and electro-Fenton (EF) process. Due to the formation of more free chlorine, the removal of NH 4 + -N and COD was in order of EO ≫ EP > EF. A new flow-through EO reactor was adopted, which was found enhanced the formation rate of free chlorine and degradation rate of pollutants, and thus performed better than that of flow-by reactor and batch reactor. By this flow-through EO process, the removal of NH 4 + -N and NO 2 - -N could reach >90% and their concentrations after treatment both meet the Water Drainage Standard for Sea Water Mariculture (SC/T 9103-2007). Meanwhile, the process had a good bactericidal performance with a lg(c/c 0 ) of −5.6. At the same time, antibiotics such as sulfadimidine (SMT) and norfloxacin (NOR) could be completely removed. The energy consumption was only 0.054 kWh/g NH 4 + -N (0.27 kWh/m 3 ), which was far more cost-effective than other oxidative processes. The new flow-through EO process has great practical application prospects for the comprehensive removal of multiple pollutants and sterilization from marine aquaculture wastewater.
研究领域:
环境科学
·
水产养殖学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yisha Xie,Xiliang Yu,Shibiao Wei,Jianan Zheng,Sangeeta Prakash,Xiuping Dong
Cod cut offs, as a by-product of cod processing, is often discarded and wasted. A cod protein gel (CP-gel) was produced from homogenized steamed cod cut offs to turn the otherwise “waste” into treasure. The effects of homogenizing conditions on the physicochemical properties of the CP-gel were investigated by assessing the gel strength, water holding capacity (WHC), rheological properties and microstructure, combined with the visual appearance. After homogenization at 80 MPa with 15 passes, the particle size was reduced to 4 μm and resulted in a gel strength of 0.63 N. The WHC of cod protein gel increased by 41% as compared to the un-homogenized sample. The secondary structures of the microfibrillar proteins changed as evidenced by the increased α-helix content by 3% via homogenization. The reduced particle size allowed particle-particle interactions and promoted protein cross-linking via both hydrophobic interaction and hydrogen bond formation. A relatively smooth and honeycombed network structure was formed with improved gel quality and physical properties. CP-gel provides a potential to utilize cod cut offs to produce value-added products.
研究领域:
食品科学
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蛋白质化学
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DOI
高影响因子
Desalination and Water Treatment
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2015
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IF 1.173
Chong Lin,Jianbo Liao,Chaohai Wei
The coagulation process by ferrous sulfate was used to pretreat coking wastewater prior to a subsequent biological treatment. The central composite Box–Behnken experimental design and response surface method were applied to evaluate and optimize the interactive effects of three operating variables, namely the initial pH, dosage of ferrous sulfate, and interaction time, on the physical and chemical performances of coagulation. Four dependent parameters, namely COD removal, cyanide removal, sulfide removal, and total oil removal, were either directly measured or calculated as responses. According to analysis of variances results, the four models proposed in this work can be used to navigate the design space, with the high regression coefficient R2 varying from 0.9158 to 0.9877. The initial pH, ferrous sulfate dosage, and interaction time had significant effects on the COD removal, cyanide removal, sulfide removal, and total oil removal due to their respective effects on chemical precipitation and coagulation removal. The synergies effect of chemical precipitation and coagulation during ferrous sulfate coagulation process controlled the treatment. A visual search of the overlaying critical response contours plot was carried out, and the results indicated the optimum conditions to an initial pH 11, ferrous sulfate dosage of 1.5 g/L, and reaction time of 120 min. The experimental date and models’ predictions agreed well. COD removal, cyanide removal, sulfide removal, and total oil removal of 15.25, 93.82, 73.36, and 4.73%, respectively, were demonstrated. The results of a B/C analysis and cost evaluation show that FeSO4 coagulation is an appropriate technology for coking wastewater pretreatment.
研究领域:
环境工程
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化学工程
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DOI
高影响因子
Water
·
2023
·Vol. 15
·期 21
·3801
·
IF 3.4
Yaoji Chen,Ruifu Chen,Xinglan Chang,Jingying Yan,Yajie Gu,Shuang Xi,Pengfei Sun,Xiaoping Dong
In order to realize the high value-added reuse of coal fly ash, a reusable Fe-modified fly ash catalyst was synthesized for ozone catalysis degradation of chemical oxygen demand (COD) in wastewater. Through enhancement of the pretreatment procedure and FeOxmodification, the resulting fly ash with Fe modification demonstrated increased specific surface area and porosity. The presence of Fe loading significantly enhances the reactivity of surface oxidizing reactive species, particularly oxygen vacancy, leading to improved adsorption and activation properties towards ozone molecules. Sodium acetate is chosen as a probe for contaminants due to its status as a small organic substance that remains resistant to further direct oxidation by ozone. This makes it suitable for evaluating the catalyst’s effectiveness in degrading chemical oxygen demand (COD). The quantitative detection of free radicals revealed the generation of •O2−was nearly 10 times that of •OH and dominated the reaction. This study showcases the potential of fly ash, an industrial byproduct, to be utilized as a cost-effective and easily prepared catalyst with consistent physical and chemical characteristics.Keywords:coal fly ash catalyst;iron oxide;ozone catalysis;radical oxidation;COD degradation
研究领域:
环境科学
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材料科学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2022
·Vol. 29
·期 34
·52175-52188
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IF 5.19
Wang Wen,Qi Liqiang,Zhang Pan,Luo Jichen,Li Jingxin
In this paper, magnetic coagulants (Fe-AFA, Fe-BFA) were prepared, by mixing acid-modified fly ash (AFA) and base-modified fly ash (BFA) with magnetic components, as adsorbents for chemical oxygen demand (COD) in desulfurization wastewater and their adsorption kinetics and mechanism are reported. BET, SEM, EDS, FTIR, XPS, magnetization intensity, and batch experiments on coagulation kinetic and adsorption isothermal characteristics of magnetic coagulants were carried out. The results show that Fe-AFA has the best COD adsorption performance and superparamagnetism, and the COD removal amounts can reach 5.69 mg/g, which is 112.43% higher than the raw fly ash. It was also found that the quasi-second-order kinetic and Langmuir equation could well describe the COD coagulation process. Thermodynamic tests results showed that the COD removal was a spontaneous, endothermic, and irreversible process. Reusability of magnetic coagulants was investigated. After five cycles, the COD removal amount of Fe-AFA was 2.74 mg/g. These findings provide a feasible method for environmental-benign utilization of fly ash as low-cost adsorbents in wastewater treatment.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Mushi Li,Xi Wang,Mingyu Ma,Juan Wu,Yang Chen,Jinbao Ou,Xinyue Chen,Qianying Zhou,Wenjing Sang,Shihong Xu,Li Cai,Dengxin Li
This study focused on the treatment of high-concentration pharmaceutical wastewater with high-salinity by pretreatment of chemical synthesis coupling with evaporation. Via chemical synthesis, this pretreatment process was developed to remove the organic matter that inhibited evaporation of wastewater, resulting in improved evaporation efficiency. Lab-scale results demonstrated that under the optimum conditions (∼pH 9, 2 mL/L demulsifier, 98 mM/L H 2 O 2 , 3 h of aeration time, 80 °C, 500 mg/L PAC, and 20 mg/L PAM), 54.9 % of COD was successfully removed by single pretreatment and 98.6 % after coupling with evaporation. Compared to the 80.1 % COD removal efficiency of direct evaporation of 100 mL with 30 min, the pretreatment process coupling with evaporation demonstrated superior performance and halved the evaporation time. A pilot-scale chemical synthesis pretreatment (120 t/d) was further developed to treat pharmaceutical wastewater of both high COD concentrations and high salinity. Under the optimal conditions determined in the lab-scale experiments, the pilot-scale achieved a COD removal efficiency of 49 % with a single pretreatment and 95.8 % after coupling with mechanical vapor recompression (MVR). Additionally, the obtained salt appeared visually clean and white compared to that obtained from the treated wastewater without pretreatment. Furthermore, to elucidate the possible mechanisms driving the efficient removal of COD, GC–MS was performed on the wastewater before and after pretreatment, and the resulting products from pretreatment in pilot-scale applications were further determined by both FTIR and GC–MS. It was proposed that the aldol condensation reaction increased the formation of carbon chains to macromolecular compounds might be the possible mechanism. This study gives insights into the future application of low-investment and low-cost technology in the treatment of high-concentration pharmaceutical wastewater with high salinity in industry.
研究领域:
环境工程
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化工技术
·
废水处理
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Yue Wang,Xiliang Yu,Weiping Zhao,Shiyi Liu,Sangeeta Prakash,Xiuping Dong
This study developed a functional gel system based on cod protein, incorporated with vitamin D-loaded bilayer microcapsules, to achieve both texture softening and nutritional enhancement through controlled moisture regulation and composite ingredients. Results demonstrated that the incorporation of microcapsules (5%-30%) significantly reduced cooking loss, gel strength, hardness, and gumminess, while improving whiteness ( p <0.05). Composite gels with 5%-15% microcapsules met the Level 6 (soft & bite-sized) criteria according to the International Dysphagia Diet Standardisation Initiative (IDDSI) testing, indicating their suitability for individuals with dysphagia. Particularly, the microcapsule content of 15% was identified as optimal. Microscopic observations confirmed that the microcapsules remained intact and demonstrated improved protein cross-linking, attributable to the addition of microcapsules enhancing hydrophobic interactions. However, excessive incorporation (30%) disrupted the three-dimensional network architecture of the gel. In vitro digestion results indicated that the soft gels with 15% microcapsules achieved a high protein digestibility of 91.83% and enhanced vitamin D bioaccessibility, compared to the free-form vitamin D with release rate of 57.46%. Furthermore, the composite gels with 15% microcapsules exhibited improved oral processing properties, including lower friction coefficient and reduced masticatory force, fulfilling both swallowing safety and nutritional requirements for populations with special dietary needs.
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产品关联
DOI
高影响因子
Desalination and Water Treatment
·
2026
·
IF 1
Yingying Zhang,Haifeng He,Xueying Wu,Xingying Tang,Yajuan Wei,Linhai Jiang,Yuanyuan Zhang,Shuhui Lan,Shiming Huang,Pengwei Ren
Accurate chemical oxygen demand (COD) determination in landfill leachate is critical for pollution assessment and treatment design but is severely compromised by heterogeneous suspended solids. Conventional filtration pretreatments improve precision but only quantify the soluble fraction, inherently underestimating the total organic load. This study introduces and systematically optimizes a novel mechanical homogenization protocol to overcome this limitation. We demonstrate that homogenizing leachate with a blender for 30 s, followed by 30-40 min of settling, effectively disperses solids into a stable, representative suspension. This optimized approach yields total COD values 26.7%–35.3% higher than filtration-based methods, with superior precision (RSD < 5%) and accuracy (spike recovery 93%–111%). Turbidity monitoring confirmed the stability of the homogenized system. Our work advances COD pretreatment practice by providing a standardized, reliable method for quantifying the total oxidizable pollutant load, ensuring data representativeness for accurate process design and environmental monitoring of landfill leachate. gravity filtration
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Yisha Xie,Feng Yang,Kangyu Zhao,Wenwen Zhang,Qingqing Liu,Yongjun Yuan
In order to obtain a soft-textured protein gel suitable for the elderly, the cod protein gel was prepared by improving the protein flexibility under ultrasound treatment. It has been found that the increase in ultrasonic power, protein flexibility, particle size, ζ-potential, surface hydrophobicity, and α-helix content of preheated cod protein exhibited an increasing trend. The improvement of protein flexibility promoted uniformity and density of the gel network, water retention, and texture properties. The flexibility of preheated cod protein increased to 0.189, the water holding capacity of the gel reached up to 99.41%, and the hardness increased to 49.12 g, as the ultrasonic power level increased to 400 W. Protein flexibility was correlated well with the cohesiveness of the gel. The storage modulus (G′) initially decreased and then increased during the heating–cooling process. The attractive forces forming between the flexible protein molecules during cooling in the ultrasound treatment groups promoted protein self-assembly aggregation and formed the cod protein gel. The gel obtained at 100–400 W could be categorized as Level 6─soft and bite-sized according to the International Dysphagia Diet Standardization Initiative (IDDSI) framework, indicating that the cod protein gel has potential as an easy-to-swallow diet for the elderly.
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产品关联
DOI
PubMed
高影响因子
Desalination and Water Treatment
·
2024
·
IF 1
Zhanmeng Liu,Xian Li,Lichun Xu,Rongrong Liu
ABSTRACT A novel composite coagulant of MFPAC was prepared from Fe 3 O 4 nanoparticles and polyaluminum chloride (PAC). Landfill leachate was pretreated by MFPAC coagulation combined with mineralized refuse adsorption. Higher COD and color removals were achieved by using the MFPAC magnetic coagulant than by adding the coagulant PAC alone. Under the working conditions of 1:3 Fe 3 O 4 /PAC mass ratios, 1.5 g/L MFPAC dosage, and pH 7.5, the removal rates of COD and color reached 63% and 67%, respectively. Infrared spectra showed that MFPAC is a type of hydroxyl-containing ligand with a nano-Fe 3 O 4 multi-core and a hydroxyl polymer of aluminum and iron that is similar complex valence and crystal structure as that of PAC. The effluent of MFPAC coagulation was further treated using mineralized refuse adsorption. Under the working conditions of <2 mm particle size of mineralized refuse, 700°C roasting temperature, 40 mg/L adsorbent dosage, and pH 9.0, the removal rates of COD and ammonia nitrogen reached up to 57% and 68%, respectively, after 12 h of treatment. Infrared spectra analysis revealed that OH, C=O, and C–O played key roles in the adsorption process. After the landfill leachate was treated with the combined process, the removal rates of COD, color, and ammonia nitrogen were enhanced to 84%, 80%, and 74%, respectively. The encouraging results demonstrated that the combined process could provide a viable alternative to the pretreatment of landfill leachate.
研究领域:
环境工程
·
废水处理
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DOI
高影响因子
危险品化学品经营许可证（带存储）