ACS Nano
·
2022
·
IF 18.027
Ling-Feng Zhang,Xing-Huan Wang,Cheng-Lu Zhang,Jaiwoo Lee,Bo-Wen Duan,Lei Xing,Ling Li,Yu-Kyoung Oh,Hu-Lin Jiang
During liver fibrogenesis, liver sinusoidal capillarization and extracellular matrix (ECM) deposition construct dual pathological barriers to drug delivery. Upon capillarization, the vanished fenestrae in liver sinusoidal endothelial cells (LSECs) significantly hinder substance exchange between blood and liver cells, while excessive ECM further hinders the delivery of nanocarriers to activated hepatic stellate cells (HSCs). Herein, an efficient nanodrug delivery system was constructed to sequentially break through the capillarized LSEC barrier and the deposited ECM barrier. For the first barrier, LSEC-targeting and fenestrae-repairing nanoparticles (named HA-NPs/SMV) were designed on the basis of the modification with hyaluronic acid and the loading of simvastatin (SMV). For the second barrier, collagenase I and vitamin A codecorated nanoparticles with collagen-ablating and HSC-targeting functions (named CV-NPs/siCol1α1) were prepared to deliver siCol1α1 with the goal of inhibiting collagen generation and HSC activation. Our in vivo results showed that upon encountering the capillarized LSEC barrier, HA-NPs/SMV rapidly released SMV and exerted a fenestrae-repairing function, which allowed more CV-NPs/siCol1α1 to enter the space of Disse to degrade deposited collagen and finally to achieve higher accumulation in activated HSCs. Scanning electronic microscopy images showed the recovery of liver sinusoids, and analysis of liver tissue sections demonstrated that HA-NPs/SMV and CV-NPs/siCol1α1 had a synergetic effect. Our pathological barrier-normalization strategy provides an antifibrotic therapeutic regimen.
研究领域:
医学
·
药理学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Zhen Wu,Hong Li,Yongde Wang,Dajian Yang,Hongjun Tan,Yong Zhan,Yong Yang,Yang Luo,Gang Chen
As a discarded byproduct of jujube ( Z . jujuba ), its seeds contain various biological components. Response surface methodology was used to optimize ultrasound-assisted heating extraction conditions of polysaccharides from Z . jujuba cv . Ruoqiangzao seeds (ZJSPs). The optimal parameters were as follows: temperature 83.1 °C, time 100 min, ultrasonic power 140 W, and water-material ratio 33.5 mL/g, allowed a maximum yield of 1.97%. One main fraction (ZJSPs-1) was successfully purified by ion-exchange and gel-permeation chromatography. With a molecular weight of 342 kDa, ZJSPs-1 was composed of arabinose, glucose, xylose, galactose and rhamnose. The presence of pyranose-form sugars as well as both α- and β-configurations was validated by FT-IR and 1 H NMR. TG/DSC indicated that ZJSPs-1 has a favorable thermal stability. XRD suggested that ZJSPs-1 exhibited both crystalline and amorphous portions. ZJSPs-1 was in aggregates of homogenous-dense honeycomb like structures observed by SEM and AFM. ZJSPs-1 possessed antitumor activities against HeLa cells in a dose- and time-dependent manner at 24 and 48 h with IC 50 of 164.6 and 87.1 μg/mL, respectively. Fluorescent microscopic and flow cytometry revealed that ZJSPs-1 inhibited the proliferation of HeLa cells through apoptosis. Z . jujuba seeds are good sources for antitumor polysaccharides in pharmaceutical and food industries.
研究领域:
食品科学
·
药学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Ming Qi,Zisheng Luo,Bin Wu,Lei Wang,Mingyi Yang,Xiaochen Zhang,Xingyu Lin,Yanqun Xu,Xihong Li,Li Li
The spatial distribution and time-course evolution of polyphenol compounds in response to exogenous light and temperature in grape berry ( Vitis labruscana cv. ‘Kyoho’) were examined. Results demonstrated that the polyphenol biosynthesis was induced via the up-regulated gene transcription levels during berry ripening. The polyphenol biosynthesis was differentially observed in skins, stems, leaves and seeds, and the more polyphenols were accumulated in seeds; and the polyphenols were significantly distributed in bottom berries of grape clusters. Additionally, the lower temperature (4 ℃) accelerated the polyphenol biosynthesis by 1.3 times after storage of 28 days, whereas the light exposure slightly inhibited the polyphenol biosynthesis of berry during storage at 25 ℃. In conclusion, the data presented in this study shed more light on deeper understanding of the spatial accumulation and time-course evolution of polyphenols in grape berry.
研究领域:
植物生理学
·
分子生物学
·
农学
✓ 已验证
DOI
高影响因子
PLANT AND SOIL
·
2013
·Vol. 370
·期 1
·345-354
·
IF 2.638
Wen Bei,Li Longfei,Liu Yu,Zhang Hongna,Hu Xiaoyu,Shan Xiao-quan,Zhang Shuzhen
Background and aims Perfluorinated compounds (PFCs) are of particular environmental concern. The migration of PFCs from soil to plants is a likely pathway for PFCs to enter the human food chain. This study aimed to investigate the uptake mechanisms of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) by maize ( Zea mays L. cv. TY2). Methods Hydroponic greenhouse experiments were performed. Results The kinetics of PFOS and PFOA uptake fitted Mechaelis-Menten equation well, suggesting their carrier-mediated influx processes. Uptake of PFOS was insensitive to metabolic inhibitors (NaN 3 and Na 3 VO 4 ). In contrast, treated with NaN 3 and Na 3 VO 4 reduced the uptake of PFOA by 83 and 43 % respectively. PFOS uptake was decreased by 31 % and 25 % when plants were treated with aquaporin inhibitors, AgNO 3 and glycerol, respectively, while aquaporin inhibitors had no effect on PFOA uptake. Anion channel blockers, 4, 4′-diisothiocyanostibene-2,2′-disolfonate (DID) and 5-nitro 2-(3-phenylpropylamine) benzoic acid (NPPB) inhibited the uptake of PFOS by 33 % and 30 %, respectively. Anion channel blocker anthracene-9-carboxylic acid (9-AC) decreased the uptake of PFOA by 28 %. No competitive uptake was found between PFOS and PFOA. Conclusions Uptake of PFOS and PFOA by maize may have different mechanisms.
研究领域:
环境科学
·
植物生理学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Jian Zhao,Jiahuan Xu,Lingxi Li,Baoshan Sun
Polymeric polyphenols in different parts (fruits, leaves and calyces) of persimmon ( Diospyros kaki L. cv. Mopan) were characterized via four different degradation methods, followed by HPLC/UPLC-UV/vis, HPLC/UPLC-Q-Exactive-Orbitrap/MS and UPLC-QqQ/MS to obtain comprehensive structural constituent unit information of polymeric polyphenols. The results indicated that persimmon polymeric polyphenols not only consisted of proanthocyanidins as terminal and extension units, but also contained anthocyanidins, flavonols and phenolic acids as terminal units. Among these different fragments detected, (−)-gallocatechin, (−)-gallocatechin-3- O -gallate, (+)-catechin and (+)-catechin-3 -O- gallate as extension units and (−)-epicatechin, (−)-gallocatechin, (−)-epigallocatechin, eight phenolic acids and four flavonols as terminal units were found for the first time in persimmon polymeric polyphenols. The molar percentages of constitutive units of persimmon polymeric polyphenols varied considerably. Compared to polymeric polyphenols in persimmon fruits and calyces, anthocyanidins, flavonols and phenolic acid units with the highest molar percentages were found in polymeric polyphenols in persimmon leaves. In contrast, the mean polymerization degrees of polymeric polyphenols in persimmon fruits, leaves and calyces were 26.45 ± 0.43, 6.23 ± 0.08 and 11.60 ± 0.07, respectively. These findings provide, for the first time, comprehensive structural information on persimmon polymeric polyphenols, and offer valuable insights into their composition and characteristics.
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Xiaohuan Zhu,Tinglan Yuan,Xiang Li,Yingjia Wang,Danli Wang,Gongshuai Song,Ling Li,Jinyan Gong
Chrysanthemi Flos , as one of the medicinal and edible herbals, contains phenols, flavonoids, and other physiologically active substances that contribute to health benefits. The stem residues also contained abundant phenolic compounds of high significance to the pharmaceutical and food industries. To valorize the currently underutilized stem residues, steam explosion (SE) pretreatment was exploited to extract the polyphenolics from Hangbaiju (HBJ) stems. After the SE pretreatment, the total phenolic and flavonoid contents of the stems were maximally increased by 1.64 and 8.71 times, respectively. The antioxidant activity also increased significantly. Notably, 3,5-CQA, linarin, luteolin, apigenin, and acacetin, which were not detected in the raw stems, could be obtained through SE. The simulated in vitro gastrointestinal digestion demonstrated that the bioaccessibility of luteolin-7-O-glucoside and apigenin-7-O-glucoside could be enhanced by SE. This study highlights that SE can promote more effective extraction of phenolics and greater antioxidant activity of HBJ stems.
研究领域:
植物学
·
食品科学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
Food & Function
·
2021
·Vol. 12
·期 16
·7440-7447
·
IF 5.396
Ying Li,Bo Chen,Hai-Yan Cao,Jing-En Li,Ling-Li Chen,Qing-Feng Zhang
Bambusa multiplex cv Fernleaf (B. multiplex) is a species of bamboo. In the present study, B. multiplex leaf extract was prepared through the resin absorption/desorption procedure and analyzed by HPLC. C-Glycosyl flavonoids are the main constituents of B. multiplex extract, and the content of isoorientin and vitexin was 51.8 and 23.1 mg g−1, respectively. Besides, the extract exhibited inhibitory activities on pancreatic lipase and α-glucosidase with IC50 values of 0.91 and 1.16 mg mL−1, respectively. The extract could bind to pancreatic lipase and showed mixed-type inhibition. An in vivo study showed that pre-administration of B. multiplex extract significantly reduced the fat absorption in rats and increased fat excretion through feces. The change in the C-glycosyl flavonoid content in feces was the same as that in the triglyceride content. The inhibitory activity of B. multiplex leaf extract on pancreatic lipase was confirmed both in vitro and in vivo.
研究领域:
药理学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 47
·33038-33046
·
IF 3.9
Gang Wang,Dan Han,Qingyu Zhang
Circulating tumour cells (CTCs), as a tumour marker, may provide more information in early diagnosis and accurate therapy of cancer patients. Electrochemical detection of CTCs has exhibited exceptional advantages. However, single-signal electrochemical detection usually has a high probability of false positives coming from interferents, operating personnel, and nonstandard analytical processes. Herein, a dual-signal strategy using anodic stripping voltammetry (ASV) and cyclic voltammetry (CV) for highly sensitive detection of CTCs was developed. When MCF-7 cells were present, aptamer DNA (DNA1)-magnetic beads (MBs) were captured by CTCs and detached from the biosensing electrodes. Following magnetic separation, polystyrene bead (PS)–CdS QDs labelled on MCF-7 cells were dissolved by HNO3 and the intensity of the oxidation peak current of Cd2+ ions was proportional to the amount of MCF-7 cells in ASV (y = 6.8929 lg Ccells + 1.0357 (Ccells, cells per mL; R2, 0.9947; LOD, 3 cells per mL)). Meanwhile, the anodic peak currents of the remaining electrode in CV were also proportional to the amount of MCF-7 cells (y = 3.7891 lg Ccells + 52.3658 (Ccells, cells per mL; R2, 0.9846; LOD, 3 cells per mL)). An ASV/CV dual-signal biosensor for electrochemical detection of CTCs was achieved, which overcame the limitations of any single-signal mode and improved the detection reliability and precision.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2025
·Vol. 41
·期 5
·1-11
·
IF 4.2
Liu Dazhao,Reymick Okwong Oketch,Luo Yuwei,Chen Xin,Che JinXin,Tao Nengguo
Cuminaldehyde demonstrates strong antimicrobial properties against several disease-causing organisms. This study investigated its antifungal effectiveness against an imazalil-resistant strain of Penicillium italicum , the pathogen responsible for postharvest blue mold disease in citrus fruits. Cuminaldehyde significantly inhibited the mycelial growth of P. italicum , with both the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) estimated at 0.5 mL/L. In vivo assays revealed that treatment with 4 × MFC cuminaldehyde in Tween-80 significantly reduced blue mold incidence in Ponkan mandarins inoculated with P. italicum . After 6 days of incubation, the disease incidence in fruits treated with 4 × MFC cuminaldehyde was approximately 72%, compared to 80% in the Prochloraz-treated fruits and 100% in the control group. The antifungal activity was attributed to the activation of oxidative stress, as evidenced by the accumulation of reactive oxygen species, damage to plasma membrane permeability in hyphal cells, and leakage of intracellular components. Additionally, treatment with 4 × MFC cuminaldehyde preserved the quality of the inoculated Ponkan mandarins by day 6. Overall, these findings indicate that cuminaldehyde is a potent antifungal alternative to current chemical fungicides used for controlling postharvest blue mold disease in Ponkan mandarins.
研究领域:
植物病理学
·
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2020
·
IF 4.679
Yi-Long Ma,Ping Sun,Jun Feng,Jia Yuan,Yue Wang,Ya-Fang Shang,Xiang-Li Niu,Shao-Hua Yang,Zhao-Jun Wei
Huangshan Gongju was extracted with organic solvents (ethanol, methanol and acetone) of different concentrations (0–90%), and the extracts’ phenolic content and antioxidant activity , as well as the correlations between them were examined. With the increasing concentration of organic solvent, the total phenolic compound (TPC) increased continuously and met its maximum at 70% acetone, whereas the total flavonoid compound (TFC) and most individual phenolics met their maximums at 70% ethanol. Similar changes occurred to the antioxidant activity, including DPPH and ABTS scavenging activities, and their maximums were respectively found at 50% acetone and 70% ethanol. The antioxidant activity correlated strongly with TPC/TFC ( r > 0.954, p < 0.01) and individual phenolics ( r > 0.886, p < 0.05), and the strongest correlations between them were mainly given by luteolin-7- O -glucoside ( r > 0.975, p < 0.001). These results suggested that high content organic solvent (50–70%) was beneficial to obtain Huangshan Gongju extracts of higher phenolic content and antioxidant activity, and 70% ethanol may be the promising solvent. Besides, phenolics were found to be the main antioxidants of Huangshan Gongju extracts, and flavonoids especially luteolin-7- O -glucoside may play more important roles in the antioxidant activity.
研究领域:
植物化学
·
食品科学
·
药用植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2019
·Vol. 43
·期 9
·e14056
·
IF 1.288
Xiao Guo,Xuedan Cao,Ailing Guo,Erhu Li
Lactic acid fermentation was applied to Ougan juice (OGJ) for the first time. This study was conducted to improve the taste of OGJ by slight lactic acid fermentation. OGJ was separately fermented at 37°C using Lactobacillus plantarum and Lactobacillus paracasei . Results showed that lactic acid bacteria strains can utilize malic acid as a carbon source, without the consumption of sugar. The observed sugar:acid ratio was highest in the L. plantarum group (3.9) that fermented for 6 hr, and in the L. paracasei group (4.5) that fermented for 12 hr, which resulted in improved sensory acceptance (taste, acidity, aroma, color) of OGJ. Moreover, compared with unfermented OGJ, there were no significant changes ( p > 0.05) in total phenolics, total flavonoids, and ascorbic acid (Vc) content in the fermented Ougan juice (FOGJ). Slight lactic acid fermentation also improved the antioxidant activity of OGJ. In particular, neohesperidin and poncirin significantly decreased in L. plantarum group ( p < 0.05), while neohesperidin significantly increased in the L. paracasei group ( p < 0.05). Overall, slight fermentation with lactic acid bacteria can be used to improve the taste of OGJ, enhancing its quality to a certain extent. Practical applications This study made full use of the metabolic characteristics of lactic acid bacteria and developed a taste balanced fermented Ougan juice, which were an extension of fruits and vegetables deep processing technology and an exploration of new ideas for the research of fermented fruits and vegetables.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Food Science & Nutrition
·
2018
·Vol. 6
·期 6
·1431-1437
·
IF 1.521
Qingyun Guo,Ke Liu,Weihui Deng,Balian Zhong,Wenxia Yang,Jiong Chun
The present investigation reported the chemical composition of cold pressed Gannan navel orange peel essential oil (EO) and its molecular distillation fraction (light phase EO), and examined their antimicrobial activity against spoiling and pathogenic microorganisms. Gas chromatography-mass spectrometry analysis identified 27 and 20 different chemical constituents in cold pressed EO and light phase EO, respectively. Limonene was the major constituent, accounting for 85.32% of cold pressed EO and 60.44% of light phase EO. Both EOs and some of their constituents showed good antimicrobial activity. Compared to cold pressed EO, light phase EO exhibited the better antimicrobial activity under weak acidic and neutral conditions. The light phase EO presented a higher antimicrobial activity after thermo-treatment at 60–100°C for 20 min than cold pressed EO. These results demonstrated that light phase EO had a potential to be used as a novel antimicrobial agent for food preservation and food processing.
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）