SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Zhenxiang Pan,Bizhen Zeng,Hongjun Lin,Jiaheng Teng,Hanmin Zhang,Huachang Hong,Meijia Zhang
While transparent exopolymer particles (TEP) has high fouling potential, its underlying fouling mechanisms have not yet been well revealed. In current work, fouling characteristics of TEP under different Ca 2+ concentrations (0 to 1.5 mM) were investigated. TEP quantification and filtration tests showed that TEP contents increased with Ca 2+ concentration, while TEP's specific filtration resistance (SFR) under the influence of Ca 2+ concentration presented a unimodal pattern. The peak of TEP's SFR reached at Ca 2+ concentration of 1 mM when SA concentration was 0.3 g·L −1 . A series of characterizations suggested that microstructure transformation of TEP particles was the main contributor to the resistance variations of TEP solution. The optical microscope observation showed that above and below the critical Ca 2+ concentration (1 mM when SA concentration is 0.3 g·L −1 in this study), the formed TEP existed in the form of c-TEP (average particle size is 0.24 μm) and p-TEP (average particle size is 1.05 μm), respectively. Thermodynamic analysis showed that the adhesion ability of c-TEP (−249,989 and − 303,692 kT) was more than 19 times than that of p-TEP (−12,905 kT), which would accelerate foulant layer formation. In addition, below the critical value, the increased SFR with Ca 2+ concentration could be explained by integrating Flory-Huggins lattice theory with the preferential intermolecular coordination. Above the critical value, the decreased SFR can be attributed to the formation of a “large-size crack structure” cake layer from the p-TEP. This study revealed fundamental mechanisms of membrane fouling caused by TEP, greatly deepening understanding of TEP fouling, and facilitating to development of effective fouling control strategies.
研究领域:
环境科学
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水处理
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膜技术
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热力学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2024
·Vol. 19
·期 9
·e0308424
·
IF 2.9
Yulin Liu,Longzhan Dong,Wenli Wu,Jiantao Ping,Jingbo Chen,Qiongzheng Hu
Hardness is one of the basic parameters of water, and a high-level hardness of drinking water may be harmful to human health. Thus, it is very important to monitor drinking water hardness. In this work, a portable lateral flow distance-based paper sensor for the semi-quantitative detection of drinking water hardness is demonstrated. In the presence of Ca 2+ /Mg 2+ , the hydrogel can be formed via the chelation between sodium alginate and Ca 2+ /Mg 2+ , inducing a phase separation process. The viscosity change of the sodium alginate solution is directly related to the Ca 2+ /Mg 2+ concentration and can be determined by the water lateral flow distance on test strips. The sensor successfully realizes the quantification of Ca 2+ and Mg 2+ in the range of 0–10 mmol L -1 and 4–20 mmol L -1 , respectively. The recoveries are found varied from 95% to 108.9%. The water hardness is acceptable for drinking if the C r values lies in the range of 0.259 to 0.419, and it is high with the C r value above 0.595. Remarkably, the performance of the sensor is comparable with the commercial kit for real water samples, which avoids the subjective judgment. Overall, this method provides a portable approach for semi-quantitative detection of drinking water hardness with the merits of convenience and low cost, which shows great potential for the potential application.
研究领域:
环境科学
·
分析化学
·
水质监测
✓ 已验证
DOI
高影响因子
3 Biotech
·
2020
·Vol. 10
·期 12
·1-9
·
IF 1.798
Zhang Jun,Tian Miao,Lv Pengmei,Luo Wen,Wang Zhiyuan,Xu Jingliang,Wang Zhongming
Long-chain fatty acids are widely used in food and chemical industries, and the enzymatic preparation of fatty acids is considered an environmentally friendly process. In the present study, long-chain fatty acids were prepared by the enzymatic hydrolysis of rapeseed oil with a genetically engineered lipase. Because thermophilic lipase has strong stability at higher temperatures, it was more suitable for the industrial production of long-chain fatty acids. Therefore, the thermophilic lipase BTL2 from Geobacillus thermocatenulatus was efficiently expressed in E. coli BL21(DE3) cells with an enzyme activity of 39.50 U/mg followed by gene codon optimisation. Experimental results showed that the recombinant lipase BTL2 exhibited excellent resistance to certain organic solvents ( n -hexane, benzene, ethanol, and butanol). The metal cation Ca 2+ and the non-ionic surfactant Triton-100X enhanced enzyme activity by 7.36% and 56.21% respectively. Moreover, the acid value of the liberated long-chain fatty acids by hydrolysing rapeseed oil was approximately 161.64 mg KOH/g at 50 °C in 24 h, the hydrolytic conversion rate was 91.45%, and the productivity was approximately 6.735 mg KOH/g h. These results suggested that the recombinant lipase BTL2 has excellent hydrolytic performance for rapeseed oil and showed great potential for the enzymatic preparation of long-chain fatty acids.
研究领域:
生物工程
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酶学
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生物技术
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2022
·
IF 15.304
Jipeng Zhang,Qimeng Wang,Bin Liu,Dan Li,Huijuan Zhang,Pengjie Wang,Jinfang Liu,Guohua Hou,Xin Li,Yu Yuan,Zekun Li,Shanan Chen,Huiling Yan,Yuan Li
The amphiphilic proteins can be used as building blocks (BBs) forming various self-assemblies. Understanding their self-assembly mechanism is important for designing novel nanomaterials . Herein, the BBs dimers were first prepared from carboxyl-abundant enzymolyzed α-lactalbumin (α-lac) at 50 °C. Then the unidentate coordination of Ca 2+ between the BBs caused a β-sheet stacking to further self-assemble into nanotubes (NTs). Compared with the traditional "one-pot" method, a step-wise new method was applied to study hydrolysis , aggregation and self-assembly processes separately. The α-lac was hydrolyzed into 11 kDa amphiphilic peptides independent of temperature while a BBs dimer was formed at 50 °C by hydrophobic interaction . Ca 2+ induced a conformational change of BBs and promoted these BBs gradually aggregate into 10 strands of filaments, which twisted into helical ribbons by electrostatic repulsion . Ca 2+ further induced the twisted helical ribbons closed into NTs driven by the reduction of line tension energy. Besides, the carboxyl-Ca 2+ coordination dominated NTs elongation in the longitudinal direction and filaments aggregation in the lateral direction with the same binding stoichiometry of 1:1 respectively. Finally, NTs successfully encapsulated curcumin and improved the viscosity of liquid food. α-Lac NTs show a high potential as a delivery system for food applications.
研究领域:
纳米技术
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生物物理学
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材料科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2024
·
IF 5.9
Zhiyu Zhang,Shuyue Liu,Dehua Zeng,Jingyi Gu,Tingting Cai,Ketong Chen,Hong Zhou,Zhi Dang,Chen Yang
Microplastics loaded with phenanthrene and derivatives are widely detected in aquatic environments, and the coating of natural minerals or organic macromolecules may change the environmental behavior of microplastics. In this study, three kinds of composites with different coverage were prepared by coating goethite on the surface of polyvinyl chloride microplastics to investigate the adsorption and desorption behavior of phenanthrene (PHE) and 1-hydroxyphenanthrene (1-OHPHE), and the effect of mucin on desorption was investigated. The results showed that goethite promoted the adsorption of PHE and 1-OHPHE by increasing the specific surface area of the composites. With the increase of the cover degree, the adsorption of PHE decreased because of the decrease in hydrophobicity; while the adsorption of 1-OHPHE initially increased and then decreased with the contributions of hydrophobic interaction and hydrogen bond. The adsorption of 1-OHPHE could be influenced by the pH and ionic strength primarily through electrostatic interactions and Ca 2+ bridging. The goethite significantly increased the desorption hysteresis for two chemicals due to the complicated pore structures and increased adsorption affinity. Mucin promoted the desorption of PHE through competitive adsorption, and inhibit the desorption of 1-OHPHE through hydrophobic interaction, hydrogen bonding and Ca 2+ bridging. This study elucidated the effects of natural minerals on the adsorption and desorption behavior of organic pollutants on microplastics, briefly discussed the effects of organic macromolecules on the desorption behavior of pollutants with different properties, and emphasized the different environmental behaviors of pollutants.
研究领域:
环境科学
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化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-24
·
IF 10.6
Wang Ying,Chen Yedan,Zhou Tao,Li Jingze,Zhang Na,Liu Na,Zhou Pinghui,Mao Yingji
Repairing bone defects is a complex cascade reaction process, as immune system regulation, vascular growth, and osteogenic differentiation are essential. Thus, developing a tissue-engineered biomaterial that caters to the complex healing process of bone regeneration remains a major clinical challenge. In the study, Ca 2+ -TA-rGO (CTAG)/GelMA hydrogels were synthesized by binding Ca 2+ using metal chelation to graphene oxide (GO) nanosheets reduced by tannic acid (TA-rGO) and doping them into gelatin methacrylate (GelMA) hydrogels. TA and rGO exhibited biocompatibility and immunomodulatory properties in this composite, while Ca 2+ promoted bone formation and angiogenesis. This novel nanocomposite hydrogel demonstrated good mechanical properties, degradability, and conductivity, and it could achieve slow Ca 2+ release during bone regeneration. Both in vitro and in vivo experiments revealed that CTAG/GelMA hydrogel modulated macrophage reprogramming and induced a shift from macrophages to healing-promoting M2 macrophages during the inflammatory phase, promoted vascular neovascularization, and facilitated osteoblast differentiation during bone formation. Moreover, CTAG/GelMA hydrogel could downregulate the NF-κB signaling pathway, offering new insights into regulating macrophage reprogramming-osteogenic crosstalk. Conclusively, this novel multifunctional nanocomposite hydrogel provides a multistage treatment for bone and orchestrates macrophage reprogramming-osteogenic crosstalk to boost bone repair. Graphical Abstract
研究领域:
组织工程
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生物材料
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骨再生
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免疫学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·Vol. 107
·期 5
·1725-1736
·
IF 5
Li Jing,Li Ziyi,Gong Hui,Ma Mengyi,Li Shuolei,Yang Huilin,Zhang Hailin,Liu Jianguo
Abstract In this study, a novel high-activity amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was identified and characterized. The recombinant enzyme was determined as a monomer with a molecular mass of 75 kDa. SaAS protein exhibited the maximum total and polymerization activities at pH 9.0 and maximum hydrolysis activity at pH 8.0. The optimum temperature for total, polymerization, and hydrolysis activities were 40, 40, and 45 °C, respectively. Under the optimal pH and temperature, SaAS had a specific activity of 108.2 U/mg. SaAS also showed excellent salt tolerance and could retain 77.4% of its original total activity at 4.0 M NaCl. The addition of Mg 2+ , Ba 2+ , and Ca 2+ enhanced the total activity of SaAS. When the conversion of 0.1 M and 1.0 M sucrose was catalyzed at pH 9.0 and 40 °C for 24 h, the ratios of hydrolysis, polymerization, and isomerization reactions were 11.9:77.4:10.7 and 15.3:53.5:31.2, respectively. The α-arbutin yield of 60.3% was achieved from 20 mM sucrose and 5 mM hydroquinone catalyzed by SaAS. Key points
• A novel amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was characterized.
• SaAS has the highest specific enzyme activity among all known amylosucrase.
• SaAS has hydrolysis, polymerization, isomerization, and glucosyltransferase activities.
Graphical Abstract
研究领域:
生物化学
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酶学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
International Journal of Bioprinting
·
2023
·
IF 8.4
Changru Zhang,Ya Ren,Weiqing Kong,Yihao Liu,Heyue Li,Han Yang,Bin Cai,Kerong Dai,Chengwei Wang,Liang Tang,Haoyi Niu,Jinwu Wang
Mesoporous bioglass (MBG) with excellent osteointegration, osteoinduction, and biodegradability is a promising material for bone regeneration. However, its clinical application is hindered by complex processing and a lack of personalization, low mechanical strength, and uncontrollable degradation rate. In this study, we developed a double-bond-functionalized photocurable mesoporous bioglass (PMBG) sol that enabled ultrafast photopolymerization within 5 s. By further integrating nanosized tricalcium phosphate (TCP) particles through three-dimensional (3D) printing technology, we fabricated personalized and highly porous PMBG/TCP biphasic scaffolds. The mechanical properties and degradation behavior of the scaffolds were regulated by varying the amount of TCP doping. In vitro and in vivo experiments verified that PMBG/TCP scaffolds slowly released SiO 4 4- and Ca 2+ , forming a vascularized bone regeneration microenvironment within the fully interconnected pore channels of the scaffold. This microenvironment promoted angiogenesis and accelerated bone tissue regeneration. Overall, this work demonstrates the solution to the problem of complex processing and lack of personalization in bioglass scaffolds, and the developed PMBG/TCP biphasic scaffold is an ideal material for bone regeneration applications with broad clinical prospects.
研究领域:
材料科学
·
生物医学工程
·
组织工程
·
骨再生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yiyang Chai,Ying Zhou,Kai Zhang,Ping Shao
Resveratrol (RES) is an important functional substance with multiple active properties. However, RES is susceptible to natural environmental conditions that reduce its bioactivity. To improve the bioavailability of RES, in this study, Catechin and Fe 3+ /Ca 2+ were selected to form supramolecules, which were then coated on the surface of hydrophobic RES nanoparticles (RES NPs) to create composite RES NPs. The obtained composite RES NPs demonstrated higher antioxidant capacity and better photo-thermal stability than RES NPs. Additionally, a pectin (PE) dietary gel was designed as a delivery carrier for RES. The results showed that the incorporation of composite RES NPs not only endowed the gels with significant dietary activity but also enhanced the texture, water retention capacity and hydrophobicity. After 28 days of storage, the retention rate of RES could be maintained above 90 % in the dietary gels. Meanwhile, the controlled release of RES was achieved in in vitro simulated digestion.
研究领域:
药学
·
食品科学
·
纳米技术
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Jia-Gen Cui,Hao Zhang,Ming-Shan Chen,Jia-Xin Wang,Yi Zhao,Jin-Long Li
Phthalates (PAEs), especially di (2-ethylhexyl) phthalate (DEHP), are generally considered to have adverse impact on nervous system. The residue of DEHP in the environment has gradually become a widely concerned environmental problem due to its widespread use in plastic items. Lycopene (LYC) as the readily available natural antioxidant is considered to have the potential to alleviate exogenous poisons-induced nerve damage. However, there is currently a lack of strategies to alleviate the neurotoxicity caused by DEHP, and it is also unknown whether LYC can alleviate the neurotoxicity caused by DEHP. The experiment demonstrated that LYC had the potential to mitigate DEHP-induced mitochondrial damage in cerebellum. DEHP induced the disorder of Ca 2+ transport in cerebellum, thereby resulting in the imbalance of protein homeostasis. Such disruption in protein homeostasis further results in the overactivation of mitochondrial unfolded protein response (UPR mt ) and mitochondrial injury. Mechanistically, LYC could alleviate the imbalance of calcium homeostasis and protein homeostasis induced by DEHP via regulating inositol 1, 4, 5-trisphosphate receptor type1 (IP3R1) and sarco/endoplasmic reticulum Ca (2+)-ATPase 2 (SERCA2), further alleviating mitochondrial damage in cerebellum. Subsequently, the present study suggested the mechanism of cerebellar injury induced by DEHP, and provided a novel approach to treating DEHP-induced neurotoxicity.
研究领域:
神经科学
·
毒理学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2024
·
IF 11.2
Ce Zhu,Miaomiao He,Juehan Wang,Yong Huang,Wei Deng,Limin Liu,Ganjun Feng,Li Zhang,Yueming Song
Photothermal therapy (PTT) is a desirable way to attain on-demand hyperthermia owing to the heat generated by photothermal materials absorbing near-infrared) light. The mild heat (42 ± 0.5 °C) can not only accelerate bone tissue regeneration but also promote the release of bioactive ions from biomaterials. Based on this one-stone-two-birds strategy, a 3D printed PEEK-graphene composite scaffold (PG) with hydroxyapatite (HA) coating (PGH) for photothermally remote control of bone regeneration was well designed in this study. The results showed that the HA coating on PGH could release Ca 2+ and PO 4 3– ions easily under NIR irradiation, which was ascribed to the mild heat generated from graphene in the composite scaffold. The mild heat and the boosted Ca 2+ /PO 4 3– release could synergistically enhance the bone regeneration ability both in vitro and in vivo . The underlying mechanism was further explored and confirmed to be closely related to the upregulation of HSP 70, by which the MAPK/ERK signaling pathway was activated selectively. The favorable results demonstrate that the 3D-printed PEEK/graphene composite scaffold is promising in the applications of bone defect repair.
研究领域:
生物材料
·
组织工程
·
生物医学工程
·
光热疗法
·
骨再生
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Yanling Liu,Qihang Cen,Kaiming Fan,Airan Hu,Xu Yuan,Shengji Xia
Efficient removal of natural organic matter (NOM) and organic micropollutants (OMPs) while preserving essential mineral salts is a critical goal of high-quality drinking water production , which has not been satisfyingly achieved by available nanofiltration (NF) membranes. In this study, a facile modification strategy was developed to enhance membrane salt/organics selectivity, purposely utilizing the advantages of sulfonic acid groups including high hydrophilicity, strong acidity and low complexation propensity for divalent cations. The post-treatment using a heated alkaline solution of taurine endowed the membrane with reduced active layer thickness, properly enlarged pore size and more favorable surface characteristics inheriting from grafted sulfonic acid groups. Compared to the control one, the optimal modified membrane exhibited a more than doubled water permeance and significantly reduced rejections of Ca 2+ and Mg 2+ , while maintaining a high removal capacity for OMPs. The membrane superiority in the rejection selectivity between mineral salts and NOM was further demonstrated for natural surface water treatment, which outperformed a variety of commercial NF membranes. Improved dual resistance to Ca 2+ -involved organic fouling and scaling was also achieved by the sulfonic acid functionalized membrane with a higher passage of Ca 2+ . This study provides insights on the fit-for-purpose enhancement of membrane performance for more efficient water treatment.
研究领域:
环境科学
·
水处理
·
膜技术
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）