ORGANIC GEOCHEMISTRY
·
2024
·
IF 2.6
Can Xie,Gangtian Zhu,Yuguang Hou,Zhiliang He
Steroid acids are unique molecular fossils composed of a steroid carbon skeleton linked to a carboxyl group. These biomarkers contain special geological and biological clues in their structures. However, the origin of steroid acids remains a subject of debate, and there is a lack of direct evidence in previous studies. Here, we present experimental evidence for the origin of steroid acids. The origin of steroid acids during biodegradation in crude oil was observed for the first time. Molecular structures of these steroid acids reveal that they arose from steranes via bacterial oxidation of sidechain terminal methyl groups. Our results suggest that microbial activities indeed contribute to the formation of carboxyl groups on the eukaryotic steroid skeletons, and we believe steroid acids are a promising biomarker for both geomicrobial processes and ancient eukaryotic life in the rock record.
研究领域:
地质生物学
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微生物学
·
古生物学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Xiao-yu Xu,Jing Chen,Zhong-xi Chen,Zhe-yan Zhang,Le-hao Jin,Jian-chao Luo,Yun-shan Zhong,Qi Zhou,Jian-chang Qian
The interindividual variability of CYP3A4 activity complicates precision pharmacotherapy, particularly for drugs with narrow therapeutic windows. To explore the relationship between CYP3A4 polymorphisms and metabolic phenotypes, this study used abemaciclib as a probe substrate within a translational framework. The in vitro platform combined a reconstituted enzyme catalysis system for high-throughput inhibitor screening with UPLC-MS/MS metabolic profiling. In vivo pharmacokinetic studies were performed in female SD rats, with optimized sampling during the elimination phase. Human CYP3A4 baculosomes were engineered using baculovirus-mediated expression in Sf21 cells to investigate genetic influences on metabolic variability. Molecular docking and dynamics simulations were also performed to investigate structural differences in inhibitory activity. Results showed that letrozole significantly inhibited CYP3A4-mediated abemaciclib metabolism, reduced its clearance and increased its area under the blood concentration-time curve (AUC) and maximum blood concentration (C max ) through mixed inhibition. Moreover, CYP3A4 polymorphisms substantially impacted abemaciclib pharmacokinetics. Kinetic simulations revealed that the CYP3A4.28 variant formed a stable complex with letrozole, enhancing inhibition, while the CYP3A4.30 variant lost catalytic activity. These findings underscored the critical role of CYP3A4 activity and genetic polymorphisms in drug metabolism and highlighted the necessity for personalized treatment approaches.
研究领域:
药理学
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分子生物学
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遗传学
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临床医学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 114
·94338-94343
·
IF 3.84
Qinghong Wang,Xia Lin,Dongmei Guo,Juanjuan Xu,Chunzhi Xuan,Cui Chen,Yingzi Fu
A sensitive, stable and stereoselective electrochemiluminescence (ECL) sensor has been designed to enantioselectively discriminate proline enantiomers by immobilizing Ru(bpy)32+–gold nanoparticles (Ru–AuNPs) and β-cyclodextrin–reduced graphene oxide (β-CD–rGO) on glassy carbon electrode. More Ru(bpy)32+ could be immobilized on the surface of electrode stably via preparing Ru–AuNPs and better stereoselectivity could be introduced to the sensor via the synthesis of β-CD–rGO. When the developed sensor interacted with proline enantiomers, obvious difference of ECL intensities towards L- and D-proline was observed, and a larger intensity was obtained from D-proline. As a result, ECL technique might act as a promising method to chiral recognition of amino acids enantiomers or chiral drugs.
研究领域:
分析化学
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生物传感
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材料科学
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DOI
高影响因子
Empirical Studies of the Arts
·
2018
·
IF 0.6
Jeremy E. Sawyer,Thalia Goldstein
Children’s drawings are implicated in their emotional, cognitive, artistic, and semiotic development, raising the question of how early educators may best facilitate drawing development. This study compared three activities to determine their relative efficacy in promoting children’s drawing. Seventy-seven preschoolers from a Head Start program were randomly assigned to one of three conditions: storybook reading, block building, or dramatic pretend play games (DPPG). Interventions were conducted over 8 weeks, and children’s free drawings during each session were rated on five dimensions: creativity, talent, spatial complexity, color, and human content. Taken together, the interventions produced significant growth in overall drawing, particularly for children who were initially more skilled at drawing. Comparisons indicated that storybook reading and block building generated significantly better overall drawing than DPPG. Story time was more beneficial than DPPG for creativity, talent, and spatial complexity, while block building was more beneficial than DPPG for children’s use of color.
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2014
·
IF 5.9
Liming Qin,Jie Zhao,Shengbin Lei,Qinmin Pan
Aquatic devices that can work on both water and oils have great scientific and practical significance, but the challenge remains in developing novel materials with excellent repellence to both water and oils. Here, we report that an artificial “strider” can float on both water and oils by using supporting legs with ultraviolet (UV) switchable wettability. The legs were fabricated by immobilizing TiO2 nanoparticles and n-dodecanethiol onto copper foams via a simple mussel-inspired process. At ambient conditions, the strider floated freely on a water surface, but it dived in water and then stood stably at the interface of water/CHCl3 after UV illumination for 2 h. The reason for this unique behavior is that the legs changed their wettability from superhydrophobicity to underwater superoleophobicity after the illumination. It was revealed that the micro/nanohierarchical structures and photosensitivity of the immobilized TiO2 nanoparticles accounted for the switchable wettability and large supporting force of the legs. The findings of this study offer an alternative strategy for fabricating smart aquatic devices that might be used for water environment protection, water resource surveillance, oil spill cleanup, and so on.
研究领域:
材料科学
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环境科学
·
表面化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Baoguo Zhang,Li Wang,Yubao Liu,Yongjian Zhang,Lipeng Zhang,Zhongning Shi
The electrochemical extraction of reactive metals, such as Li, Mg, Al, Ti, and rare-earth metals, from different electrolyte systems at low temperatures has been a longstanding challenge in the field of electrochemical extractive metallurgy. Water is an excellent green solvent; therefore, the electrodeposition of aqueous solutions of reactive metals would be more sustainable than traditional approaches involving molten salts and organic solvents. Inspired by the successful application of water-in-salt electrolytes for battery chemistry, we attempted the electrochemical extraction of metallic Li from water via its deposition on different substrates. We found that Li can be electrodeposited in the form of Li-based alloys and hydroxides, as identified by X-ray diffraction and X-ray photoelectron spectroscopy analysis, under certain conditions, and the mechanisms behind the observed electrochemical behaviour are discussed using different characterisation techniques and density functional theory calculations. In addition, the prospects for the electrodeposition of reactive metals from aqueous solutions are considered, and suggestions for further study are made. Thus, this work provides important insights regarding the electrochemical extraction of reactive metals from water at room temperature, and we believe that this work forms a basis for the development of safer batteries, as well as advanced electrodeposition and electrometallurgy technology.
研究领域:
材料科学
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电化学
·
冶金学
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DOI
高影响因子
PLoS One
·
2021
·Vol. 16
·期 2
·e0246347
·
IF 3.24
Siyi Chen,Zhigang Chen,Yan Shen
In view of the current severe situation of illegal land use in China, it is of great significance to explore the impact of the evolution and promotion of land law enforcement effectiveness, which will improve China’s land law enforcement system and effectively curb illegal land use. This paper explains the changes and enhancements of the effectiveness of land law enforcement since the implementation of China’s National Land Supervision System in terms of the deterrence, difficulty, and strength of land law enforcement, and explores the role of land law enforcement effectiveness in changing illegal land use behaviors from a theoretical level. Then, a corresponding empirical test was carried out using the provincial panel data of Mainland China from 2007 to 2016. The results show that the increase in land law enforcement deterrence and strength will help reduce the number of illegal land use cases, but it will drive the lawbreakers to "commit major cases in desperation", leading to the increase of the degree of illegal land use; and with the decrease of land law enforcement difficulty, the degree of illegal land use will be significantly reduced. At the end of this paper, several policy suggestions are put forward to effectively curb the illegal land use from the perspective of improving the land law enforcement system and enhancing the effectiveness of law enforcement.
研究领域:
环境法
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土地资源管理
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公共政策
·
城市规划
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DOI
高影响因子
Science Advances
·
2021
·Vol. 7
·期 3
·
IF 14.143
Lixue Tang,Jin Shang,Xingyu Jiang
Electronic tattoos have great potential in health and movement sensing applications on the skin. However, existing electronic tattoos cannot be conformal, sticky, and multilayered at the same time. Here, we have achieved multilayered integration of the electronic tattoo that is highly stretchable (800%), conformal, and sticky. This electronic tattoo can enable the crease amplification effect, which can amplify the output signal of integrated strain sensors by three times. The tattoo can be transferred to different surfaces and form a firm attachment, where no solvent or heat is needed. The tattoo fabrication is straightforward and scalable; a layer-by-layer strategy and two materials (metal-polymer conductors and the elastomeric block copolymer) are used to fabricate the circuit module with desirable numbers of layers within the tattoo. A three-layered tattoo integrating 1 heater and 15 strain sensors is developed for temperature adjustment, movement monitoring, and remote control of robots.
研究领域:
柔性电子学
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可穿戴传感器
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生物医学工程
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柔性机器人
·
人机交互
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DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Zhong Wang,Ya Zhao,Yuyu Zhao,Yi Zhang,Xiaohong Yao,Ruiqiang Hang
Copper, a frequently used additive of implant materials, can alter macrophage phenotype thus directing the fate of the implants. Exosomes, secreted by mammalian cells, can target to recipient cells and mediate their functions. However, whether exosomes derived from macrophages upon copper ion stimulation can modulate angiogenesis, a key index for implant osseointegration, is still unclear. Herein, the influence of copper ions on macrophage-derived exosome secretion, ingestion behavior by endothelial cells, and angiogenic-induction ability is investigated. The results show copper ions (0–100 μM) have little influence on the secretion of macrophage-derived exosomes. Endothelial cells can uptake the exosomes from all the groups in a time-dependent manner. The exosomes have little influence on endothelial adhesion and proliferation, but can upregulate angiogenic ability of endothelial cells in vitro and in vivo , which may be related to trafficking of integrin β1. The results provide insight into the effect of copper ions on immunomodulatory mechanism of macrophages, which is important for implant design from the perspective of material compositions.
研究领域:
免疫学
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材料科学
·
细胞生物学
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DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2014
·Vol. 174
·期 8
·2818-2826
·
IF 1.687
Shu H.,Xu L.,Li Z.,Li J.,Jin Z.,Chang S.
Banana is an important tropical fruit worldwide. Parthenocarpy and female sterility made it impossible to improve banana varieties through common hybridization. Genetic transformation for banana improvement is imperative. But the low rate that banana embryogenic callus was induced made the transformation cannot be performed in many laboratories. Finding ways to promote banana somatic embryogenesis is critical for banana genetic transformation. After tobacco arabinogalactan protein gene NtEPc was transformed into Escherichia coli (DE3), the recombinant protein was purified and filter-sterilized. A series of the sterilized protein was added into tissue culture medium. It was found that the number of banana immature male flowers developing embryogenic calli increased significantly in the presence of NtEPc protein compared with the effect of the control medium. Among the treatments, explants cultured on medium containing 10 mg/l of NtEPc protein had the highest chance to develop embryogenic calli. The percentage of lines that developed embryogenic calli on this medium was about 12.5 %. These demonstrated that NtEPc protein can be used to promote banana embryogenesis. This is the first paper that reported that foreign arabinogalactan protein (AGP) could be used to improve banana somatic embryogenesis.
研究领域:
植物学
·
遗传学
·
分子生物学
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2017
·
IF 4.208
Jingjing Du,Yuyan Zhang,Lina Liu,Mingxiang Qv,Yanna Lv,Yifei Yin,Yinfei Zhou,Minghui Cui,Yanfeng Zhu,Hongzhong Zhang
ZnO nanoparticles is one of the most used materials in a wide range including antibacterial coating, electronic device, and personal care products. With the development of nanotechnology, ecotoxicology of ZnO nanoparticles has been received increasing attention. To assess the phototoxicity of ZnO nanoparticles in aquatic ecosystem , microcosm experiments were conducted on Populus nigra L. leaf litter decomposition under combined effect of ZnO nanoparticles and visible light radiation . Litter decomposition rate, pH value, extracellular enzyme activity , as well as the relative contributions of fungal community to litter decomposition were studied. Results showed that long-term exposure to ZnO nanoparticles and visible light led to a significant decrease in litter decomposition rate (0.26 m −1 vs 0.45 m -1 ), and visible light would increase the inhibitory effect (0.24 m -1 ), which caused significant decrease in pH value of litter cultures, fungal sporulation rate, as well as most extracellular enzyme activities. The phototoxicity of ZnO nanoparticles also showed impacts on fungal community composition, especially on the genus of Varicosporium , whose abundance was significantly and positively related to decomposition rate. In conclusion, our study provides the evidence for negatively effects of ZnO NPs photocatalysis on ecological process of litter decomposition and highlights the contribution of visible light radiation to nanoparticles toxicity in freshwater ecosystems .
研究领域:
生态学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Lirui Hou,Yuhan Fu,Chong Zhao,Lihong Fan,Hongbo Hu,Shutao Yin
Ciprofloxacin (CIP) and enrofloxacin (ENR) are veterinary antibiotics commonly utilized to treat and prevent animal diseases. Environmental and dietary antibiotic residues can directly and indirectly affect the reproductive development of animals and humans. This article investigated the reproductive toxicity of CIP in male zebrafish, showing that it could decrease the spermatogonial weight and damage the spermatogonial tissue. The sex hormone assays showed that CIP decreased fshb and lhb gene expression and plasma testosterone (T). In addition, transcriptome analysis indicated that the effect of CIP on zebrafish might be related to the endocrine signaling pathways. ENR, which was selected for further study, inhibited mouse Leydig (TM3) and Sertoli (TM4) cell proliferation and caused cell cycle arrest. The sperm concentration, serum luteotropic hormone (LH) and follicle-stimulating hormone (FSH), and T levels decreased in adolescent mice after ENR treatment for 30d in vivo. Hematoxylin and eosin (H&E) staining showed that ENR exposure potentially induced testicular injury, while the real-time quantitative PCR (qPCR) results indicated that ENR inhibited the mRNA expression of key genes in the Leydig cells (cyp11a1, 3β-HSD, and 17β-HSD), Sertoli cells (Inhbβ and Gdnf) and spermatogenic cells (Plzf, Stra8 and Dmc1). In conclusion, these findings indicated that ENR exposure might influence the development of the testes of pubescent mice.
研究领域:
兽医学
·
生殖生物学
·
毒理学
·
内分泌学
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DOI
PubMed
高影响因子
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