SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Zihan Wang,Xinru Wang,Ziqiang Li,Min Wang,Wenwen Fan,Chengmin Zha,Li Zhou,Xinzhong Zhang,Zongmao Chen,Fengjian Luo
Residual pesticides in agricultural environments, including soil and irrigation water, can be taken up by plants, and thus pose a potential risk to food safety. Although tolfenpyrad has been widely used in tea plantations, limited information is available on its root uptake and fate in tea plants ( Camellia sinensis L.). Exploring the mechanisms involved is crucial for understanding the migration and accumulation of tolfenpyrad in tea plants, particularly in the edible parts. In this study, root uptake of tolfenpyrad and its subsequent translocation, distribution, and metabolism in tea seedlings were investigated. The results indicated that the passive transport and apoplastic pathway dominated the root uptake of tolfenpyrad. After uptake, tolfenpyrad distributed predominantly in the cell walls (90.8–92.0 %) of roots, resulting in limited upward translocation in water-soluble fractions through transpirational pull, with translocation factor values far <1 (TF stem/root = 0.115–0.453 and TF leaf/stem = 0.039–0.184). Similar accumulation patterns were observed for the carboxylated metabolite PT-CA as well as hydroxylated metabolite PT-OH. Interestingly, the subcellular distribution of PT-CA in stems was much different from that of the parent tolfenpyrad: PT-CA mainly distributed in the stem cell walls (41.72 %) and cell organelles (56.18 %) at 3 h, then gradually transferred into the cell-soluble fractions (33.07 %) after 120 h. Results from the present study indicated limited upward translocation of tolfenpyrad with its main metabolites to leaves. This finding helps to alleviate concerns about environmental residual tolfenpyrad in tea consumption and provides valuable information for the safety evaluation of tolfenpyrad.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 17
·5677
·
IF 4.927
Jiachun Lu,Yanyan Cao,Yani Pan,Sifan Mei,Gang Zhang,Qiang Chu,Ping Chen
The chemical substances responsible for thekokumitaste of green tea infusion are still unclear. Here, we isolated thekokumicompound-containing fractions from green tea infusion through ultrafiltration, and the majorkokumicompounds were characterized as γ-Glu-Gln and γ-Glu-Cys-Gly (GSH) through ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS). The results indicated that peptides and amino acids were essential compounds in the kokumi-enriched fractions for conducting the sense ofkokumi. L-theanine had an enhancing effect on thekokumitaste of green tea infusion, which was confirmed in the sensory reconstitution study. Thus, peptides, especially γ-Glu-Gln and GSH, are the majorkokumicompounds in green tea infusion, which has the potential of improving the flavor of tea beverages.Keywords:kokumipeptides;sensory-guided;identification;characterization;recombination
✓ 已验证
DOI
PubMed
高影响因子
Agronomy-Basel
·
2024
·Vol. 14
·期 1
·156
·
IF 3.3
Zuguo Xi,Huiyan Jia,Yifan Li,Jinqing Ma,Mengqian Lu,Zhihui Wang,Dexu Kong,Wei-Wei Deng
Tea is a healthy beverage made from the leaves of the tea plant [Camellia sinensis(L.) O. Kuntze]. The tea plant is a perennial evergreen plant that is widely distributed in tropical and subtropical regions. PR proteins (pathogenesis-related proteins, PRs) are defense-related proteins induced under pathogenesis-related conditions. Currently, there are a few studies on PRs in plants. Tea leaf color mutants have been of wide interest to scientists as special materials. A tea plant displaying variegated spontaneous albinism found in a local tea plantation in Huangshan City, Anhui Province, China, was used as the material sample in this study. An analysis of preexisting transcriptomic and proteomic data revealed the significant upregulation of three classes of PRs. In order to investigate the correlation between PRs and variegated tea plants, a series of studies were conducted onPRgenes. The results revealed the identification of 17CsPR1, 3CsPR4and 31CsPR5genes in tea plants using bioinformatics methods, and their structures and promoter sequences. The expression of threePR1, twoPR4and onePR5genes was determined to be induced in stress treatment experiments involving mechanical damage, insect bites, low temperature treatment, and fungal infections. Additionally, the ribonuclease activity of CsPR4 was successfully verified. This is the first study to report the ribonuclease activity of CsPR4 in tea plants. The results can serve as a reference for future studies on PRs in tea plants, offering new insights into information on albinism in tea plants.Keywords:identification;function analysis;pathogenesis-related protein;variegated tea plants
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Jieyao Yu,Chi-Tang Ho,Zhi Lin,Yin Zhu,Zhihui Feng,Dejiang Ni,Shengchun Zeng,Xuehong Zeng,Yijun Wang,Jingming Ning,Liang Zhang,Xiaoting Zhai,Xiaochun Wan
The volatile fraction was isolated from the premium and common grade of Lu’anGuapian green tea infusion by solvent-assisted flavor evaporation distillation. With the application of aroma extract dilution analysis, a total of 52 aroma-active compounds were revealed in the flavor dilution (FD) factor area of 32-8192. Besides, five additional odorants with higher volatility were identified using solid-phase microextraction. The aroma profiles, FD factors, and quantitative data of premium Guapian (PGP) and common Guapian (CGP) showed apparent differences. The intensity of the flowery attribute was significantly higher in PGP than in CGP, while cooked vegetable-like was the most outstanding odor note in CGP. The recombination experiment and the omission test of PGP clarified that dimethyl sulfide, (E,E)-2,4-heptadienal, (E)-β-ionone, (E,Z)-2,6-nonadienal, 2-methylbutanal, indole, 6-methyl-5-hepten-2-one, hexanal, 3-methylbutanal, γ-hexalactone, methyl epijasmonate, linalool, geraniol, and (Z)-3-hexen-1-ol were the key odorants of PGP tea infusion. The omission and addition tests of flowery odorants manifested that (E)-β-ionone, geraniol, and (E,E)-2,4-heptadienal, with higher odor activity values in PGP than in CGP, contributed to the flowery attribute most. The difference in the concentration of the abovementioned odorants with flowery odor notes could be one of the main factors which led to the difference in aroma quality between the two grades of Lu’an Guapian.
✓ 已验证
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF AGRONOMY
·
2023
·
IF 5.2
Die Hu,Wei Chen,Aiqing Miao,Xiaoyan Qiao,Hongling Xia,Chengying Ma
Volatile blends contain numerous information about the emitters. Volatile organic compounds (VOCs) have been used as biomarkers to monitor agri-products’ state during postharvest management. We placed tea shoots at 15 °C or 25 °C after plucking and wondered if the emitted VOCs may reflect the freshness of these plucked tea shoots or differentiate tea shoots experiencing different postharvest treatments . Headspace solid-phase microextraction combined with gas chromatography-mass spectrometry was applied in this research to profile volatiles that were released from plucked tea shoots. Twenty-nine VOCs were found from all tea shoots samples, and the composition of released VOCs varied under different temperatures. Assessed by PLS-DA and two-way ANOVA, fourteen volatiles were selected as putative markers. All the markers were detected under the low-temperature condition, but not under the room-temperature condition. Transcriptome analysis showed that low temperature caused less variation in gene expression compared to room temperature. Many genes in volatile biosynthetic pathways had similar expression trends in either condition, while some changes occurred with a delay in the low-temperature environment. The study advances the understanding of the physiological changes of tea shoots subjected to temperature-dependent postharvest treatments .
✓ 已验证
产品关联
DOI
高影响因子
SCIENTIA HORTICULTURAE
·
2023
·
IF 4.3
Wei Ran,Qinghui Li,Xinlong Hu,De Zhang,Zhi Yu,Yuqiong Chen,Mingle Wang,Dejiang Ni
“High mountain, cloud and mist produce high-quality tea”; however, the specific mechanism has not been fully understood. To explore the influence of environmental factors on the quality of tea fresh leaves, we firstly selected the same variety of tea plant growing at different altitudes in Xuan'en County, China. And then, based on the analyses of the physical and chemical properties of the soil where they grew, we comprehensively analyzed the composition and content of volatile and non-volatile substances in these tea fresh leaves harvested from 27 sampling sites. The relative contents of linalool and its oxides I and II, phenylethanol and nerol in paddy soil were higher than those in red soil and yellow soil, but the contents of acids and esters were significantly lower than those in other soils ( P < 0.05). Benzoic acid content and palmitic acid content are relatively high in red soil and yellow soil, respectively. The amino acid content of tea fresh leaves produced in paddy soil is significantly lower than that in red soil and yellow soil, while the contents of catechins and flavonol glycosides were significantly higher than that in other soils ( P < 0.05). The relative contents of EGCG, ECG and C in paddy soil were significantly higher than those in yellow soil and red soil. The content of alkaloids in red soil is high. With the increase of altitude, the content of amino acids gradually increased, and the catechins content showed a trend of first decreasing and then increasing; the phenolic acid content changes in accordance with catechins with the elevation; the flavonoids content (alcohols) glycosides did not change obviously in each altitude range. These results are helpful for us to fully understand the influence of environmental factors on the quality of fresh tea leaves.
✓ 已验证
产品关联
DOI
高影响因子
Horticultural Plant Journal
·
2020
·
IF 1.524
Mingyue Zhao,Binbin Cai,Jieyang Jin,Na Zhang,Tingting Jing,Jingming Wang,Yuting Pan,Zixiang Zhou,Yifan Zhao,Yingying Feng,Feng Yu,Mengting Zhang,Yating Li,Zhonghua Liu,Chuankui Song
Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants. Plant eugenol glycosides have been identified and shown to make important contributions to fruit floral quality. However, the details of their biosynthesis and metabolism in tea plants are still unknown. Here, eugenol glucoside was unambiguously identified as a native metabolite in the tea plant, and its biosynthesis was shown to be induced by low temperature treatment. Through the analysis of UGTs induced by low temperature, the glycosyltransferase CsUGT78A15 was identified in tea, and its encoded protein was shown to catalyze the glucosylation of eugenol. V max / K m ratios showed that eugenol was the most suitable substrate for CsUGT78A15. Sugar donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose, followed by galactose and glucuronic acid. The expression of CsUGT78A15 was correlated with the accumulation of eugenol glucoside in different tissues and genotypes of tea. Down-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress, indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold stress. The identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside.
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2016
·
IF 4.495
Xu Huanhuan,Wang Ya,Chen Yana,Zhang Pan,Zhao Yi,Huang Yewei,Wang Xuanjun,Sheng Jun
Galloylated catechins, as the main secondary metabolites in the tea plant, including (-)-epigallocatechin-3-gallate and (-)-epicatechin-3-gallate, comprise approximately three-quarters of all the tea plant catechins and have stronger effects than non-galloylated catechins, both on the product quality in tea processing and the pharmacological efficacy to human beings. The subcellular localization of galloylated catechins has been the primary focus of studies that assess biosynthesis and physiological functions. Classical histochemical localization staining reagents can not specifically detect galloylated catechins; thus, their subcellular localization remains controversial. In the present study, we generated a monoclonal antibody (mAb) against galloylated catechins, which can be used for the subcellular localization of galloylated catechins in the tea plant by immunohistochemistry. Direct ELISA and ForteBio Octet Red 96 System assay indicated the mAb could recognize the galloylated catechins with high specificities and affinities. In addition, tea bud was ascertained as the optimal tissue for freezing microtomic sections for immunohistochemistry. What’s more, the high quality mAbs which exhibited excellent binding capability to galloylated catechins were utilized for the visualization of them via immunohistochemistry. Our findings demonstrated that vacuoles were the primary sites of localization of galloylated catechins at the subcellular level.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 2
·260
·
IF 4.1
Wenli Wang,Xiaogui Zhou,Qiang Hu,Qiuhong Wang,Yanjun Zhou,Jingbo Yu,Shibei Ge,Lan Zhang,Huawei Guo,Meijun Tang,Xin Li
Tambocerus elongatus(Shen) (Hemiptera: Cicadellidae) is a devastating insect pest species ofCamellia sinensis, significantly affecting the yield and quality of tea. Due to growing concerns over the irrational use of insecticides and associated food safety, it is crucial to better understand the innate resistance mechanism of tea trees toT. elongatus. This study aims to explore the responses of tea trees to different levels ofT. elongatusinfestation. We first focused on the primary metabolism and found that the amino acid levels decreased significantly with increasingT. elongatusinfestation, while sugar accumulation showed an opposite trend. Moreover, secondary metabolite analysis showed a significant increase in flavonoid compounds and lignin content afterT. elongatusinfestation. Metabolomics analysis of the flavonoid compounds revealed a decrease in the proanthocyanidin level and an increase in anthocyanidin glycosides (anthocyanins and their derivatives) afterT. elongatusinfestation.T. elongatusinfestation also caused a decrease in the abundance of non-ester catechins and an increase in the abundance of ester catechins. Furthermore, the gene expression analysis revealed that transcripts of genes involved in flavonoid biosynthesis, such asCsCHI,CsF3H,CsF3′H,CsFNS,CsFLS, andCsUFGT, were down-regulated, while genes involved in the lignin pathway were up-regulated by insect infestation, suggesting that lignin probably plays a pivotal role in tea plant response toT. elongatusinfestation. Analysis of the expression of related genes indicates that the jasmonate (JA) pathway primarily responds to leafhopper damage. These findings suggest that the lignin pathway and JA play a preferential role in tea plant response toT. elongatus. Furthermore, the production of saccharides and the accumulation of anthocyanin glycosides in the flavonoid metabolic pathway are critical during this stress response. Further exploration of the roles of anthocyanin glycosides and lignin in tea tree resistance could provide a theoretical basis for understanding the defense mechanism of tea trees againstT. elongatusdamage.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plant Methods
·
2018
·Vol. 14
·期 1
·1-8
·
IF 4.269
Alagarsamy Karthikeyan,Shamala Lubobi Ferdinand,Wei Shu
Background Camellia sinensis var. sinensis is widely grown for tea beverages that possess significant health promoting effects. Studies on tea plant genetics and breeding are hindered due to its recalcitrance to Agrobacterium -mediated genetic transformation. Among the possible reasons, oxidation of phenolics released from explant tissues and bactericidal effects of tea polyphenols during the process of transformation play a role in the plant recalcitrance. The aim of the present study was to alleviate the harmful effects of phenolic compounds using in - planta transformation. Results Two-month old seedlings of tea cultivar “Nong Kangzao” were infected at the hypocotyl with wild type Agrobacterium rhizogenes and maintained in an environment of high humidity. 88.3% of infected plants developed hairy roots at the wounded site after 2 months of infection. Our data indicated that transgenic hairy root induction of tea can be achieved using A. rhizogenes following the optimized protocol. Conclusion With this method, composite tea plants containing wild-type shoots with transgenic roots can be generated for “in root” gene functional characterization and root-shoot interaction studies. Moreover, this method can be applied to improve the root system of composite tea plants for a better resistance to abiotic and biotic stresses.
✓ 已验证
DOI
PubMed
高影响因子
PLANT BIOTECHNOLOGY JOURNAL
·
2025
·
IF 10.5
Mingyue Zhao,Jingming Wang,Xinyuan Hao,Jieyang Jin,Junwei Tang,Yueyue Wang,Mengting Zhang,Tingting Jing,Wilfried Schwab,Ting Gao,Xinchao Wang,Chuankui Song
Summary Cold stress severely limits tea plant ( Camellia sinensis ) productivity, yet the molecular mechanisms underlying cold adaptation remain elusive. Here, we identified a cold-inducible glycosyltransferase, CsUGT71A60, through integrative genome-wide association studies (GWAS) and proteomic profiling. Natural variation in CsUGT71A60 was strongly associated with cold tolerance, as evidenced by linkage disequilibrium analysis of flanking SNPs. Functional characterization revealed that CsUGT71A60 specifically catalyses the glycosylation of cis-zeatin to form cis-zeatin 9-O-glucoside in vitro and in vivo. Overexpression of CsUGT71A60 in Arabidopsis enhanced cold tolerance and agronomic traits, including germination rate, tiller number and seed weight, while delaying flowering. Transient silencing of CsUGT71A60 in tea plants disrupted cis-zeatin homoeostasis, impairing antioxidant defences and osmotic regulation under cold stress. Mechanistically, the transcription factor ARR (TEA021099) directly binds to CRM elements in the CsUGT71A60 promoter, activating its expression to fine-tune cytokinin signalling. This study unveils a dual-function glycosyltransferase that orchestrates stress tolerance and developmental plasticity, offering a strategic target for breeding climate-tolerance crops without yield penalties.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2018
·Vol. 42
·期 4
·1352-1367
·
IF 5.415
Tingting Jing,Na Zhang,Ting Gao,Mingyue Zhao,Jieyang Jin,Yongxian Chen,Miaojing Xu,Xiaochun Wan,Wilfried Schwab,Chuankui Song
Plants emit a variety of volatiles in response to herbivore attack, and (Z)-3-hexenol and its glycosides have been shown to function as defence compounds. Although the ability to incorporate and convert (Z)-3-hexenol to glycosides is widely conserved in plants, the enzymes responsible for the glycosylation of (Z)-3-hexenol remained unknown until today. In this study, uridine-diphosphate-dependent glycosyltransferase ( UGT ) candidate genes were selected by correlation analysis and their response to airborne (Z)-3-hexenol, which has been shown to be taken up by the tea plant. The allelic proteins UGT85A53-1 and UGT85A53-2 showed the highest activity towards (Z)-3-hexenol and are distinct from UGT85A53-3, which displayed a similar catalytic efficiency for (Z)-3-hexenol and nerol. A single amino acid exchange E59D enhanced the activity towards (Z)-3-hexenol, whereas a L445M mutation reduced the catalytic activity towards all substrates tested. Transient overexpression of CsUGT85A53-1 in tobacco significantly increased the level of (Z)-3-hexenyl glucoside. The functional characterization of CsUGT85A53 as a (Z)-3-hexenol UGT not only provides the foundation for the biotechnological production of (Z)-3-hexenyl glucoside but also delivers insights for the development of novel insect pest control strategies in tea plant and might be generally applicable to other plants.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）