ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2020
·Vol. 28
·期 12
·15032-15042
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IF 3.056
Zhao Zhilin,Xu Limei,Wang Yong,Li Bihan,Zhang Wenming,Li Xiaochen
Silver nanoparticles (Ag-NPs) are widely used in daily life and inevitably discharged into the aquatic environment, causing increasingly serious pollution. Research on the toxicity of Ag-NPs is still in infancy, little information is available on the relationships between oxidative stress and antioxidant, as well as damaging degrees of Ag-NPs to cellular structural components of Chlamydomonas reinhardtii ( C. reinhardtiii ). In the present study, we revealed the toxicity mechanism of C. reinhardtii under Ag-NPs stress using flow cytometry (FCM), metabolic methods, and transmission electron microscopy. The results showed that the chloroplasts were damaged and the synthesis of photosynthetic pigments was inhibited under Ag-NPs stress, which inhibited the growth of C. reinhardtii . Meanwhile, Ag-NPs also caused C. reinhardtii to produce excessive reactive oxygen species (ROS), increased malondialdehyde content and changed the permeability of cell membrane, resulting in the acceleration of internalization of Ag-NPs. The decrease of cell size and intracellular chlorophyll autofluorescence was observed with FCM. To deal with the induced excessive ROS that could lead to lethal and irreversible structure damage, C. reinhardtii activated antioxidant enzymes including superoxide dismutase and peroxidase. This study provides new information for better understanding the potential toxicity risks of Ag-NPs in the aquatic environment.
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高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2023
·
IF 5.1
Miao Yang,Jingyi Ma,Sihui Yu,Kunpeng Xie,Weidong Zhang,Endong Zhang,Qingzhi Wang,Xi Xie,Zheng Gong
Naphthenic acids (NAs) serve as natural components of petroleum, and have turned into essential pollutants in wastewater discharged from various petroleum industries . Some NAs could elicit toxicity towards multiple organisms. Microalgae from culture collections and the indigenous algal strains have been tested for their capacity to tolerate and biodegrade NAs. It is necessary to comprehensively assess the physiological response of microalgae to NA exposure, especially the glycerolipid metabolism. In this study, the representative algal strain for wastewater treatment , Chlorella pyrenoidosa and the model green alga , Chlamydomonas reinhardtii , were used to evaluate their responses to a model NA, cyclohexanecarboxylic acid (CHCA), involving growth, photosynthetic activity , cell morphology and glycerolipid metabolism. The results showed that, C. pyrenoidosa cells were not affected in growth performance and cell structure during 9 days of CHCA exposure. However, the growth of C. reinhardtii was retarded, and the surface of the enlarged cells got damaged within 3 days of CHCA treatment. Their photosynthetic activity both remained stable when subjected to CHCA stress. More importantly, the fatty acyl profiles of total lipids, membrane lipids and neutral lipids were all remodeled and C18:3n3 in the two algae serves as the potential biomarker in response to CHCA treatment. Thus, C. pyrenoidosa presented more superior tolerance to CHCA exposure relative to C. reinhardtii , and they both reconstructed the glycerolipid components for acclimation to CHCA stress. Overall, glycerolipid remodeling is crucial for green microalgae to accommodate the NAs-contaminated environment. This study offers valuable insights into the potential tolerance and physiological response of green algae to NA stress, especially in glycerolipid remodeling, which will benefit improved monitoring of NAs pollution in aquatic ecosystems and effective treatment of NA-contaminated wastewater.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Sijie Wei,Jun Cao,Xinyue Ma,Jie Ping,Chao Zhang,Tan Ke,Yurui Zhang,Yue Tao,Lanzhou Chen
Microalgae have been used for the removal of heavy metals or synthetic organics; however, the simultaneous removal of both types of compounds is always technically difficult. In this study, a green algae , Chlamydomonas reinhardtii , was first used to simultaneously remove hexavalent chromium [Cr(VI)] and o-nitrophenol (ONP), and the balance among biomass, oxidative damage and removal rate was also investigated. The results showed that treatment with Cr(VI) or ONP decreased the photosynthetic and superoxide dismutase activities and increased the production of reactive oxygen species (ROS) and malondialdehyde content. However, combined treatment with Cr(VI) (≤4 mg/L) and ONP (≤15 mg/L) significantly decreased ROS generation and alleviated cell damage in C. reinhardtii . In addition, the removal rates of Cr(VI) and ONP by C. reinhardtii cells significantly increased from 37.4% to 54.9% and from 35.8% to 45.9%, respectively, and the cells could be reused at least four times. Moreover, the increased acidity in the medium and Cr(VI) reductase content in C. reinhardtii caused Cr(VI) to be reduced to Cr(III). The addition of an exogenous antioxidant decreased the removal rates of Cr(VI) and ONP. These results indicated that the presence of Cr(VI) could induce ROS generation in C. reinhardtii and enhance ONP degradation, which consumed ROS, alleviated cell damage, and thus benefited Cr(VI) reduction. As a result, C. reinhardtii could be used as a theoretical candidate for the simultaneous removal of combined Cr(VI) and ONP contamination.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jing Jiang,Xinwei Li,Kaiguang Yang,Wangyin Wang,Xupeng Cao,Can Li
Formate, which can be produced from CO 2 with green energy, has received increasing attentions as a carbon source for bioconversion. Microalgae have the native advantages of use captured light energy to facilitate the formate assimilation. However, the low efficient natural conversion and inhibition to the electron transfer chain obstacle its applications’ development. Here, using green microalga C. reinhardtii 21gr as the model, an approach to improve formate-based biomass and protein production was raised and verified through isotope labeling experiments, and the pathway of formate which presented in photosynthetic organisms was related to amino acids. By the enhancement of chloroplast glyceraldehyde-3-phosphate dehydrogenase (cGAPDH) expression, together with the adaptive laboratory evolution (ALE) over regular lethal dose of formate, the yields of the biomass and proteins were promoted with 34 % and 55 % to the wild type, respectively. Indicated by the photosynthetic activity variations, it was proposed that the balance of cellular energy and reduction power is necessary for microalgae to achieve high productivity with formate. Further investigated by proteomics, 6-phosphogluconate dehydrogenase (6-PGD) was identified as the key enzyme to formate and nitrogen’s co-assimilation.
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Environmental Science-Nano
·
2026
·
IF 5.6
Erqin Yin,Chao Cheng,Qiyong Hao,Yixuan Li,Xiaochen Li
As an important class of engineered nanomaterials, CuO nanoparticles have found broad use across various areas, while their toxicity has drawn attention. Although previous studies have reported the environmental fate of CuO particles and their ecotoxicity to algae, whether particle size modulates the aquatic toxicity of CuO particles and how algae coordinate detoxification responses under particle stress remain incompletely understood within a unified framework. In this study, physiological and transcriptomic analyses were combined to compare the toxic effects of two CuO particle size fractions, CuO-NPs (40 nm) and CuO-SMPs (150–250 nm), on Chlamydomonas reinhardtii (C. reinhardtii), and to investigate the detoxification responses of this alga at both cellular and molecular levels. The results revealed that, under the tested conditions, the CuO-NPs fraction induced stronger growth inhibition, photosynthetic damage, and oxidative stress than the CuO-SMPs fraction. At 50 mg L−1, the growth inhibition rates caused by CuO-NPs and CuO-SMPs reached 40.84% and 31.60%, respectively. RNA-seq analysis revealed 1054 differentially expressed genes, comprising 302 upregulated and 752 downregulated ones, which were mainly enriched in oxidation–reduction processes and oxidoreductase activity. By integrating physiological endpoints with transcriptomic responses, we further outlined a relatively complete detoxification route employed by C. reinhardtii under CuO particle stress, involving peroxisome regulation, activation of the ascorbate–glutathione cycle, phytochelatin chelation, and ABC transporter-mediated compartmentalized sequestration. This study provides new insight into the molecular detoxification mechanisms of microalgae under nanoparticle stress and contributes to a more complete understanding of the bidirectional interaction between CuO-NPs and aquatic microalgae.
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BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Kaixiang Ying,Xiaofan Xing,Zhen Wang,Qisheng Tian,Qinwen Zhang,Changhong Liu,Lei Zheng
NLCs stabilize Fe 1 /NC to prevent aggregation in culture media for over 7 days. • NLCs bypass the cytomembrance barrier to enable efficient Fe 1 /NC cellular. • Fe 1 /NC-NLCs increase biomass, lipid and carbohydrate accumulation. • Fe 1 /NC-NLCs scavenge ROS, elevate POD activity by 35% and CAT activity by 200%. • Fe 1 /NC-NLCs regulate related genes and significantly modulate carbon flux. To overcome the inherent bottlenecks of low metabolic efficiency and biomass yield in microalgal biomanufacturing, this study presents an innovative strategy using single-atom iron dispersed within nanoliposomes (Fe 1 /NC-NLCs). Traditional metal additives often suffer from severe aggregation and toxicity. Here, nanoliposomes not only prevented single-atom iron aggregation but also ensured superior aqueous dispersion and effective cellular uptake by Chlamydomonas reinhardtii ( C. reinhardtii ). Functioning as highly dispersed bioactive regulators rather than conventional catalysts, these composites profoundly rewired cellular metabolism. Consequently, upon continuous treatment with an optimized dosage of 8.62 g/L Fe 1 /NC-NLCs over a 7-day cultivation period, microalgal biomass concentration significantly increased from 1.27 to 1.50 g/L. Crucially, the treatment orchestrated a metabolic shift toward energy-rich storage compounds, achieving carbohydrate and lipid yields of 203.35 mg/(L·d) and 613.36 mg/(L·d), which were approximately 2.2-fold and 2.0-fold higher than the control, respectively. Mechanistically, Fe 1 /NC-NLCs maintained cellular redox homeostasis by amplifying endogenous antioxidant enzymes, specifically POD and CAT, whose activities reached 646.8 U/g and 224.3 U/g, respectively. Transcriptomic analysis further revealed that this physiological enhancement was coupled with the upregulation of nitrogen assimilation, pyruvate metabolism, and amino acid biosynthesis. Ultimately, by efficiently coordinating carbon and nitrogen fluxes, this novel nanoplatform maximizes microalgal bioproduction, demonstrating profound potential for sustainable biofuel and biochemical applications. Download: Download high-res image (177KB) Download: Download full-size image
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