Food Bioscience
·
2023
·
IF 5.2
Xianghui Yan,Xiaofeng Gong,Zheling Zeng,Dongman Wan,Jiaheng Xia,Maomao Ma,Junxin Zhao,Pengbo Wang,Shuhong Zhang,Ping Yu,Deming Gong
In this study, Cinnamomum camphora seed kernel protein isolate (CPI)−dextran (DX) model system was used to investigate the impacts of reaction temperatures (70−100 °C) and times (1−4 h) on the structure, functional properties and volatile compounds of Maillard reaction products (MRPs). The formation of MRPs was confirmed by SDS-PAGE, ultraviolet absorption and degree of glycation. Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy showed that the secondary structure of MRPs tended to be more regular than native CPI. Moreover, MRPs had a lower free sulfhydryl group content and higher surface hydrophobicity than native CPI. Fluorescence spectra revealed that the tryptophan residues of CPI were transferred to a more hydrophobic microenvironment. The solubility and emulsifying stability of CPI were decreased, while the foaming activity, foaming stability, emulsion stability and apparent viscosity of CPI were significantly improved ( p < 0.05) after Maillard reaction. Furthermore, MRPs exhibited significantly improved ( p < 0.05) thermal stability and ABTS + • radical scavenging activity than native CPI, while the DPPH + radical scavenging activity and ferric reducing power were decreased. In addition, volatile compounds, including aldehydes, ketones, benzenes and esters, were identified in MRPs. These results provide a theoretical guidance for future studies on CPI-DX conjugates .
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高影响因子
ENVIRONMENTAL RESEARCH
·
2023
·
IF 8.3
Tianyu Huang,Meng Lai,Zhenwei Lin,Ruiqi Luo,Xuezheng Xiang,Haozhe Xu,Ning Pan,Zhaojiang Zuo
Cyanobacterial blooms cause serious environmental issues , and plant secondary metabolites are considered as new algaecide for controlling them. Cinnamomum camphora produces a wide spectrum of terpenoids and has 4 main chemotypes, including linalool, camphor , eucalyptol and borneol chemotype. To develop the new cyanobacterial algaecide by using suitable chemotype of Cinnamomum camphora and the main terpenoids, we analyzed the terpenoid composition in the 4 chemotype extracts, evaluated the algicidal effects of the extracts and their typical monoterpenoids on Microcystis aeruginosa , and investigated the algicidal mechanism of the stronger algicidal agents. Among the 4 chemotypes, eucalyptol and borneol chemotype extracts exhibited stronger algicidal effects. In the 4 chemotype extracts, monoterpenoids were the main compounds, of which linalool, camphor, eucalyptol and borneol were the typical components. Among the 4 typical monoterpenoids, eucalyptol and borneol showed stronger algicidal effects, which killed 78.8% and 100% M. aeruginosa cells, respectively, at 1.2 mM after 48 h. In 1.2 mM eucalyptol and borneol treatments, the reactive oxygen species levels markedly increased, and the caspase-3-like activity also raised. With prolonging the treatment time, M. aeruginosa cells gradually shrank and wrinkled, and the cell TUNEL fluorescence intensity and DNA degradation gradually enhanced, indicating that the lethal mechanism is causing apoptosis-like programmed cell death (PCD). Therefore, eucalyptol and borneol chemotype extracts and their typical monoterpenoids have the potential for developing as algaecides to control cyanobacteria through triggering apoptosis-like PCD.
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高影响因子
FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Xianghui Yan,Shibo Liang,Ting Peng,Guohua Zhang,Zheling Zeng,Ping Yu,Deming Gong,Shuguang Deng
Cinnamomum camphora seeds are abundant in China. For high value utilization of Cinnamomum camphora seed kernel (CCSK) protein, the effects of phenolic compounds on physicochemical and functional properties of protein isolate (PI) from CCSK were investigated in this study. A total of 12 phenolic compounds in CCSK have, for the first time, been tentatively identified by HPLC-ESI-QTOF-MS 2 . PI had a higher level of protein content compared to that of protein-phenolic complex (PPC). The formation of PPC resulted in a reduction of free sulfhydryl groups content and surface hydrophobicity as well as an increase of particle size and zeta potential . Fourier-transform infrared spectroscopy showed that the contents of secondary structure of PI and PPC were not obviously different. However, a remarkable increase in β -sheet and a decrease in random coil of PPC compared to PI were found by circular dichroism . Differential scanning calorimetry analysis revealed that PPC was thermally more stable than that of PI. PI and PPC exhibited superior essential amino acid composition and similar protein subunits. Furthermore, PPC exhibited higher solubility, better foaming and emulsifying properties than those of PI. These results suggested that the protein products from CCSK may be used as natural functional materials in the food industry.
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Foods
·
2024
·Vol. 13
·期 6
·968
·
IF 4.7
Mengqiang Ye,Zhixin Wang,Xianghui Yan,Zheling Zeng,Ting Peng,Jiaheng Xia,Junxin Zhao,Weiyun Wang,Deming Gong,Ping Yu
Cinnamomum camphoraseed kernel protein isolate (CPI) has attracted increasing attention due to its sustainability and potential applications. This study aimed to investigate the effects of freeze-drying (FD), vacuum-drying (VD), and spray-drying (SD) on the physicochemical and functional properties of CPI. The morphology observation results showed that the SD-CPI, SD-CPI, and VD-CPI were spherical, lamellar, and massive, respectively. Compared to FD and SD, VD had more impact on the color, surface hydrophobicity, intermolecular disulfide bonds, intrinsic fluorescence, and thermal stability of CPI. Fourier transform infrared spectroscopy (FTIR) analyses showed that among three CPI samples, VD-CPI had the highest content of β-sheet but the lowest contents of α-helix and β-turn. At different pH values, the solubility, emulsification, and foaming properties of VD-CPI were inferior to those of FD-CPI and SD-CPI. These results provide useful information on the changes in the physicochemical and functional properties of CPI subjected to different drying methods, and offer theoretical guidance for the production and use of CPI in the food industry.
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高影响因子
MOLECULES
·
2024
·Vol. 29
·期 4
·796
·
IF 4.2
Zaizhi Liu,Haibin Liao,Yanting Dai,Yanlong Qi,Zhengrong Zou
The ultrasonic-assisted extraction (UAE) method was employed to separateCinnamomum camphoraproanthocyanidin-rich extracts (PCEs). This extraction process was optimized by the Box–Behnken design, and the optimal conditions, on a laboratory scale, were as follows: an ethanol concentration of 75%, a liquid-to-solid ratio of 24 mL/g, an ultrasonic time of 39 min, and an ultrasonic power of 540 W. Under the obtained conditions, the PCE yield extracted by UAE was higher than that from heat reflux extraction and soaking extraction. An ultra-performance liquid chromatography–tandem mass spectrometry analysis was employed to characterize the phloroglucinolysis products of theC. camphoraPCEs, by which epigallocatechin, catechin, epicatechin, and (−)-epigallocatechin-3-O-gallate were identified as the terminal units; epigallocatechin, epicatechin, and (−)-epigallocatechin-3-O-gallate were recognized as extension units. TheC. camphoraPCEs possessed higher anti-ultraviolet activity in vitro compared with the commercially available sunscreen additive of benzophenone with respect to their ethanol solutions (sun protection factor of 27.01 ± 0.68 versus 1.96 ± 0.07 at a concentration of 0.09 mg/mL) and sunscreens (sun protection factor of 17.36 ± 0.62 versus 14.55 ± 0.47 at a concentration of 20%). These results demonstrate thatC. camphoraPCEs possess an excellent ultraviolet-protection ability and are promising green sunscreen additives that can replace commercial additives.Keywords:proanthocyanidin-rich extracts;ultrasonic-assisted extraction;characterization;anti-ultraviolet radiation activity
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高影响因子
Future Foods
·
2025
·
IF 8.2
Xianghui Yan,Miao Luo,Zheling Zeng,Yujing Yang,Xiansi Gao,Yifang Gao,Ding Cheng,Guohua Zhang,Deming Gong,Ping Yu
Cinnamomum camphora seed kernel (CCSK) is a rich source of plant-based food ingredients, with high levels of medium-chain oil, protein and carbohydrates. Despite its nutritional value, approximately 12% of carbohydrates go to waste during the extraction process of CCSK oil. In this study, a novel crude CCSK polysaccharide (CCSKP) was extracted from the aqueous phase of the aqueous-extraction process for CCSK oil. The chemical compositions, physicochemical properties, prebiotic activities, and potential application in yogurt of CCSKP were evaluated. The results showed that CCSKP contained 64.70 ± 1.27% sugar, 11.33 ± 1.12% protein and 9.45 ± 0.33% uronic acid, and the glucose content of CCSKP was 91.10%. The hydrolysis rates of CCSKP by simulated gastric fluid and α-amylase were 4.45 ± 0.18% and 5.90 ± 0.13%, respectively. The assessment of prebiotic activities demonstrated that CCSKP effectively promoted the growth and acidifying activity of probiotic strains ( L. acidophilus, B. longum, B. infantis and B. adolescentis ) while enhancing the production of short-chain fatty acids. Furthermore, the addition of CCSKP was found to improve the pH and titratable acidity, water-holding capacity and texture properties (hardness, gumminess and chewiness) of yogurt. These findings suggest that CCSKP may function as an effective prebiotic and offer a natural, plant-based solution to meet the growing demand for functional foods that support digestive health.
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高影响因子
Foods
·
2022
·Vol. 11
·期 19
·3066
·
IF 5.561
Xianghui Yan,Xiaofeng Gong,Zheling Zeng,Maomao Ma,Junxin Zhao,Jiaheng Xia,Meina Li,Yujing Yang,Ping Yu,Deming Gong,Dongman Wan
TheCinnamomum camphoraseed kernel (CCSK), with high contents of medium-chain oil (~59%) and protein (~19%), is an excellent source for a plant-based food ingredient. To broaden the application of the protein isolate (PI) from CCSK in the food industry, the Maillard reaction products (MRPs) were prepared by PI and dextran (DX) under mild wet-heating conditions (60 °C, 5 h), and the structural and functional properties of the PI-DX conjugates were investigated. The covalent bond between PI and DX was confirmed by the degree of grafting and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Compared with the heated PI, the PI-DX conjugates had more ordered structure, with the decreased random coil content. The changes in tertiary structure of PI-DX conjugates were reflected by the results of intrinsic fluorescence and surface hydrophobicity. Moreover, PI-DX conjugates showed better solubility, emulsifying properties, thermal stability and antioxidant activities. These results provided a theoretical basis for the development of PI-based MRPs with desirable characteristics.Keywords:plant protein;Maillard reaction;conjugate;structural property;functional property
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高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2021
·Vol. 16
·期 2
·510-522
·
IF 4.102
Qingyun Luo,Zhengfeng Tian,Tiefeng Zheng,Sun Xu,Yuandan Ma,Shuzhen Zou,Zhaojiang Zuo
Cinnamomum camphora (L.) J. Presl can produce an abundance of terpenoids, and has four main chemotypes: linalool, eucalyptol, camphor, and borneol. Antioxidant activity and the chemical composition of extracts from the four chemotypes were investigated during spring (May), summer (July), and autumn (November) with the aim of developing natural antioxidants using suitable chemotypes of C. camphora in suitable seasons, together with their main terpenoids. The main antioxidant terpenoids were identified. The four chemotype extracts showed strong scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radicals as well as O 2 – · and ·OH in July, due to the fact that their terpenoid content was at its highest during this month. Among the four chemotypes, the strongest scavenging activity was mainly detected in the linalool chemotype extracts, which had the highest terpenoid content. Among the ten main terpenoids in C. camphora extracts, ocimene, linalool, β -pinene, longifolene and eucalyptol showed stronger scavenging activity against DPPH free radicals, and ocimene exhibited the strongest scavenging activity. Extracts from the four chemotypes of C. camphora and their main terpenoids have the potential for development as natural antioxidants, with summer as the best season, linalool chemotypes as the optimum choice, and ocimene as the optimum compound. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd
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高影响因子
PHARMACEUTICAL CHEMISTRY JOURNAL
·
2020
·Vol. 54
·期 2
·154-161
·
IF 0.538
Ying-Jiao Liu,Li-Juan Xie,Lan Xiao,Li-Min Gong,Ta-Si Liu,Nai-Hong Chen
Cinnamomum camphora Chvar. Borneol (CCCB) and Cinnamomum camphora Chvar. Cineol (CCCC) are two different chemical types of C. camphora . The clinical application of these chemical types of CC shows big differences, but sometimes they are confused because of the similarity of morphological features. To identify the two types of CC and to analyze the differences of compositions, their morphological characteristics were investigated by microscopic techniques and the chemical components were determined by GC-MS analysis and GC internal standard method. Distinguishing features of morphological characteristics were the shape, petiol, gland, and smell. Microscopic study of the transverse sections of leaves showed 13 – 15 columns xylem vessel in the leaves of CCCB, 11 – 13 columns xylem vessel, and more obvious and thicker cell walls of fiber cells in the leaves of CCCC. From this research, GC-MS analysis of essential oil of the leaves could provide mainly differential characteristics to distinguish between CCCB and CCCC. The total of 33 compounds were identified in the essential oil of CCCB leaves and the main component was d -borneol (55.11%), while d -borneol in CCCC leaves was not the main component and the amount of d -borneol might be related to the microstructure of gland. L(-)-camphor and d-borneol were quantitatively analyzed by GC with FID detector, using methyl salicylate as the internal standard. Each gram of the total essential oil contains 0.68 g of d -borneol in CCCB, and each gram of the total essential oil contains 0.150 and 0.015 g of L(-)-camphor in CCCB and CCCC, respectively, from Hunan province. Other habitats showed the same trend. This research was aimed to establish a method based on the microscopic, GC-MS, and GC techniques to identify and quantify CCCB and CCCC compositions, for providing scientific data to ensure with accuracy the origin of CCCB and CCCC plant material.
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高影响因子
Foods
·
2026
·Vol. 15
·期 6
·1054
·
IF 6
Rongping Fu,Xianghui Yan,Zheling Zeng,Yujing Yang,Pinpin Zhang,Yuling Lin,Deming Gong,Ping Yu
TheCinnamomum camphoraseed kernel (CCSK) shows great promise as a natural source of bioactive alkaloids. However, there is little data about recovering alkaloids from CCSK by-products after oil extraction using an aqueous method. This study aimed to establish an efficient technology for enriching CCSK alkaloids (including magnoflorine, lindoldhamine and N,N-methyldomesticinium) using macroporous resin technology. The results showed that XR918C resin was the most suitable adsorbent due to its high adsorption/desorption capacity for CCSK alkaloids. The adsorption process was best described by Langmuir isotherm models and pseudo-second-order kinetics; it was spontaneous and physical in nature. The optimum procedure for CCSK alkaloids enrichment using XR918C resin was as follows: for adsorption, the injection flow rate and sample volume were 2.0 BV/h and 7.0 BV, respectively; for desorption, the eluent type, elution flow rate and volume were 80% ethanol, 2.0 BV/h and 6.0 BV, respectively. Furthermore, the scale-up of the CCSK alkaloid enrichment process was performed under optimal conditions. Following the 10-fold scale-up enrichment, the content of CCSK alkaloids was raised 4.41-fold, with a recovery rate of 89.19 ± 0.01%. After nine regeneration cycles, the efficiency of the XR918C resin remained stable, indicating its good reusability. In addition, CCSK alkaloids exhibited strong in vitro antioxidant activity. This study provides a useful reference for the industrial-scale enrichment of CCSK alkaloids.
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Qingwen Zhu,Yujing Yang,Zheling Zeng,Ting Peng,Xianghui Yan,Junxin Zhao,Jiaheng Xia,Ping Yu,Xuefang Wen,Deming Gong
The study aimed to investigate the effects of processing methods on oil yield, chemical composition, physicochemical properties, antioxidant activity and volatile compounds of Cinnamomum camphora seed kernel oil (CCSKO). The processing methods used were hot pressing (HP), cold pressing (CP), solvent extraction (SE), aqueous extraction (AE), sub-critical fluid extraction (SCFE) and supercritical fluid extraction (SFE). The results showed that the extraction efficiencies from different processing methods ranged from 44.48% to 97.93%, with the highest efficiency from SCFE. All CCSKO samples were found to be rich in medium-chain saturated fatty acids (> 96%), and capric acid-capric acid-lauric acid accounting for the largest proportion of triacylglycerols (77.54%−78.74%). However, the total polyphenol content, δ-tocopherol content and antioxidant activity of AE, α-tocopherol and γ-tocopherol content of CP, and squalene content of SCFE were all significantly higher than those of the other samples. Additionally, the thermal behaviors and spectral profiles of all samples were similar. Furthermore, there were significant differences in the volatile compounds of CCSKO with different processing methods ( p < 0.05). Linalool , 2-nonanol, and ethyl caprate were determined to be the key aroma compounds of CCSKO. Overall, the results provide a theoretical reference for further processing of CCSKO.
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Environmental Technology & Innovation
·
2025
·
IF 7.1
Chenyang Zhang,Jihai Zhou,Meng Na,Shangqi Xu,Xiaoping Li,Shoubiao Zhou
This study examined the effects of three exogenous plant growth regulators on the resistance physiology of Cinnamomum camphora ( C. camphora ) seedlings and their cadmium (Cd) absorption and transport under Cd stress. To this end, the efficacies of several plant growth regulators in the remediation of Cd-polluted soil were compared. This paper also provides a theoretical foundation for the ecological restoration of C. camphora and the incorporation of plant growth regulators in mining-abandoned and industrially polluted lands. The findings revealed that the application of 20 mg kg –1 3-indole acetic acid (IAA), gibberellin (GA 3 ), and 6-benzylaminopurine (6-BA) facilitated plant growth and enhanced the photosynthesis of C. camphora seedlings. Furthermore, the antioxidant capacity of C. camphora seedlings was improved: the superoxide dismutase activity decreased by 1.0 %, 11.7 %, and 24.4 %. Similarly, the ascorbic acid content decreased by 39.0 %, 17.9 %, and 22.2 %, respectively. These changes effectively boosted the ability of C. camphora seedlings to restore Cd-contaminated soil, consequently promoting Cd accumulation in plant organs and lowering soil Cd residue by 30.8 %, 12.9 %, and 18.1 %, respectively. The treatments also enabled Cd accumulation in the aboveground and underground parts of the seedlings, effectively increasing their resistance against Cd. These results demonstrate that applying plant growth regulators is a promising approach for strengthening phytoremediation in contaminated soils.
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