Foods
·
2024
·Vol. 13
·期 6
·827
·
IF 4.7
Jiayin Liang,Yuqing Xi,Jiaming Li,Shugui Xu,Yongqin Zheng,Meirong Xu,Zheng Zheng,Xiaoling Deng
Citri ReticulataePericarpium, especially the pericarp ofCitrus reticulataBlanco cv. Chachiensis (PCRC), is an important edible and medicinal ingredient for health and pharmacological properties. Citrus Huanglongbing, a devastating disease that currently threatens the citrus industry worldwide, is caused by a phloem-limited alpha-proteobacterium, “CandidatusLiberibacter asiaticus” (CLas). The industry of cultivar Chachiensis has been suffering from HLB. Although HLB affected the quality of citrus fruit, whether the quality of PCRC was affected by HLB remains unclear. In this study, we compared the metabolite profiles between HLB-affected and healthy PCRC from three sources: fresh, 6-month-old, and 9-year-old PCRC, through the untargeted LC–MS method. Compared to healthy controls, various types of bioactive compounds, mainly flavonoids, terpenoids, alkaloids, coumarins, polysaccharides, and phenolic acids, accumulated in HLB-affected PCRC, especially in the HLB-affected 9-year PCRC. In particular, isorhamnetin, isoliquiritigenin, luteolin 7-O-beta-D-glucoside, limonin, geniposide, pyrimidodiazepine, scoparone, chitobiose, m-coumaric acid, malonate, and pantothenic acid, which contributed to the pharmacological activity and health care effects of PCRC, were highly accumulated in HLB-affected 9-year-old PCRC compared to the healthy control. Multibioassay analyses revealed that HLB-affected 9-year-old PCRC had a higher content of total flavonoids and total polyphenols and exhibited similar antioxidant capacity as compared to healthy controls. The results of this study provided detailed information on the quality of HLB-affected PCRC.
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Yu-shi Huang,Ya-ling An,Wen-jie Zhao,Chun-qian Song,Zi-jun Tang,Lin Feng,Zhen-wei Li,Xiao-kang Liu,De-an Guo
The global consumption of Citri reticulatae pericarpium (Chenpi, CP) and Citrus reticulata 'chachi' (Guangchenpi, GCP) has been experiencing a steady increase, driven by its extensive applications in healthcare, flavoring, and therapeutic fields. While the two varieties exhibit analogous macroscopic characteristics and therapeutic efficacies, rigorous market differentiation and identification are imperative owing to their distinct botanical origins, qualitative specifications, and especially significant price discrepancy. A comprehensive strategy integrating HPTLC and UHPLC analyses with machine learning was implemented in this research to discriminate between CP and GCP and to conduct a comparative content analysis of three characteristic flavonoids in the two materials. The discrimination results of eight models upon HPTLC data reveal that all models except Tree achieved 100% discrimination accuracy. (2S)-Narirutin, Nobiletin, and Tangeretin, three compounds contributed to the differentiation were quantified and exhibited distinct content differences in CP and GCP samples. This study could provide simple and scientific guidance for the authentication of the two varieties.
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产品关联
DOI
PubMed
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2021
·Vol. 32
·期 6
·1152-1161
·
IF 3.373
Xiaofang He,Sisi Huang,Mengmei Wu,Menghua Wu,Ying Zhang,Zhiguo Ma,Li Liu,Hui Cao
Introduction Citri Reticulatae Pericarpium Viride (Qing Pi in Chinese) is a clinically effective Chinese herb, which contains biologically valuable flavonoids. Qing Pi is divided into two commodity specifications, Si Hua Qing Pi (SHQP) and Ge Qing Pi (GQP), based on the harvesting time. The flavonoid contents in Qing Pi from different origins and commodity specifications may vary significantly, which will affect their therapeutic functions. Thus, it is crucial to set up a reliable and comprehensive quality evaluation method for flavonoid analysis in Qing Pi. Objectives We aimed to establish a rapid and sensitive ultrahigh-performance liquid chromatography method coupled with diode-array detection and high-resolution mass spectrometry (UPLC-DAD-HRMS) for identification and quantification of ten flavonoids in Qing Pi. Chemometric methods were further applied to distinguish Qing Pi of different origins and specifications. Methodology An UPLC-DAD-HRMS method was developed for the simultaneous separation and quantification of ten flavonoids in 46 batches of Qing Pi samples from different sources in China. Chemometric approaches were applied to discriminate Qing Pi from different origins and commodity specifications. Results The chemometric procedures (i.e., hierarchical clustering analysis and principal component analysis) were employed to identify the differences of Qing Pi samples with different origins and commodity specifications. The results showed that the contents of ten flavonoids in Qing Pi samples of different origins were significantly different, and the same results were found out between SHQP and GQP. Conclusions This study provides a chemical basis for quality control of Qing Pi.
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DOI
高影响因子
危险品化学品经营许可证（带存储）