IMMUNOLOGIC RESEARCH
·
2025
·Vol. 73
·期 1
·1-20
·
IF 3.1
Xu Cong,Lu Chenqi,Wang Wu,Wang Zhimin,Qiu Yang,Han Min,Yang Jun,Li Shanglin
Autoimmune hepatitis (AIH) is an immune-mediated liver disease that currently lacks viable drug treatment methods. This study is to explore the role of piceatannol (PIC) in ConA-induced AIH and the related mechanisms. A mouse model of AIH was established by injecting ConA (i.v.), and PIC was administered as an intervention. The protective effect of PIC was evaluated by the liver function, liver pathology, and serum levels of inflammatory factors. Subsequently, network pharmacology was used to predict the pathways and targets of PIC in the treatment of AIH, and the predicted results were validated using flow cytometry, molecular docking, surface plasmon resonance (SPR) and so on. Finally, the immunosuppressive effect of PIC was further validated in a mouse heart transplantation model. PIC can improve liver function decline and reduce pathological liver damage, as well as inhibit the increase of serum inflammatory factor levels in mice with AIH induced by ConA. The protective effect is achieved by suppressing the immune activity of T cells and macrophages through binding to c-Jun. PIC can also extend the survival of cardiac allografts and inhibit acute rejection reactions. These results indicated that PIC can significantly improve ConA-induced AIH in mice by inhibiting the immune activity of T cells and macrophages through binding to c-Jun. PIC can also extend the survival of cardiac allografts in mice and inhibit acute rejection responses. The above results indicated that PIC may serve as a promising immunosuppressant and be effective for AIH.
研究领域:
免疫学
·
肝病学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xionghui Ma,Jianping Li,Yunbin Liu,Yali Yuan,Guobao Xu
Herein, a concanavalin A (ConA) electrochemical sensor was constructed via a sandwich mode. Firstly chitosan functionalized graphene oxide (CS/GO) composite was introduced on the of glassy carbon electrode (GCE) as the substrate. D–mannose (D-man) was immobilized on the surface of CS/GO via Schiff base reaction. Due to the specific affinity between mannose and ConA, the prepared D–man/CS/GO sensing interface could specifically capture ConA which could further react with mannose residues of HRP and form a sandwich configuration. Based on the catalytic effect of horseradish peroxidase (HRP) to H 2 O 2 , a ConA electrochemical sensor with enzyme catalytic amplification was constructed by taking hydroquinone as electrical mediator. The morphology of CS/GO was investigated by transmission electron microscope and Fourier transform infrared spectroscopy. Electrochemical techniques including electrochemical impedance spectroscopy and differential pulse voltammogram (DPV) were used for reveal the characteristics of the sensing platform. Benefit from the high surface area of GO and enzyme catalytic amplification of HRP, the peak current from DPV is linearly dependent on the ConA concentration ranging from 5 × 10 −9 to 5 × 10 −7 mol/L with a detection limit of 1.24 × 10 −9 mol/L. The results demonstrated that the proposed sensor might exploit a new direction for determination of ConA.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Nano Energy
·
2022
·
IF 19.069
Zhou Zhou,Peng Wang,Jiawei Li,Congyu Wang,Junhuan Chen,Liyang Zhu,Haitao Zhu,Dun Zhang
Gram-negative bacteria (Gnb) such as sulfate-reducing bacteria (SRB), as the main culprits of microbiological induced corrosion (MIC) in marine environment, cause severe problems for marine construction. Thus, the rapid and sensitive detection of Gnb is vital for environmental monitoring and metal corrosion protection . Herein, we developed a microbiosensor system based on triboelectric nanogenerator (TENG), by which Gnb such as SRB in the solution can be detected instantly. In the system, concanavalin A (ConA)-modified indium tin oxide glass (ITO-ConA) captures Gnb by specific recognition between ConA and Gnb; ConA-functionalized carboxyl multi-walled carbon nanotubes with high-conductivity serve as signal amplification material; and TENG provides a stable power supply to the microbiosensor system. Variation of Gnb concentration leads to changes in the adsorption capacity of CNT-ConA, which further results in the variation of resistance. Thus, the microbiosensor system could effectively detect the bacteria concentration based on the monitoring of output voltage. In addition, by integrating alarm circuit with microbiosensor, the detection results can be can be directly indicated by light emitting diode . Desulforibrio sp. Huiquan2017 was chosen as typical SRB for validating the performance of the as-obtained self-powered microbiosensor. In the detection range of 3 × 10 3 to 3 × 10 7 CFU mL −1 , the correlation between peak voltage and bacteria concentration exhibits linear relation. Meanwhile, the detection mechanisms were further elucidated by adopting molecular simulations . This work not only offers an effective strategy for detecting Gnb, but also provides useful insights into the development of portability, specificity, and stability of TENG-based sensor systems .
研究领域:
材料科学
·
环境监测
·
生物传感器
·
海洋工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Qiong-Lin Wang,Hui-Fang Cui,Xiaojie Song,Shuang-Fei Fan,Li-Li Chen,Meng-Meng Li,Zong-Yi Li
Carcinoembryonic antigen (CEA), a glycoprotein, is a wide-spectrum tumor marker for cancer diagnosis. Developing simple, stable, and low-cost methods for CEA detection is of great importance. In this work, a novel, label-free, and antibody-free electrochemical sandwich CEA biosensor was developed based on concanavalin A (ConA) and a DNA aptamer against CEA. Horse radish peroxidase (HRP) was labeled on the sandwich structure for signal production and amplification. Both the CEA and the HRP bind to ConA through sugar-lectin interactions. Under optimum conditions, the detection linear range was from 5 to 40 ng mL −1 CEA, with a detection limit of 3.4 ng mL −1 , lower than the threshold level in human serum for cancer patients (∼10 ng mL −1 ). The biosensor is reproducible, accurate, specific, and stable, with RSD value of 3% for inter-biosensors, and relative error of <13% compared with those obtained by a commercial diagnostic kit. No obvious responses were observed toward interfering proteins. Storage did not cause obvious signal diminishment for the biosensor. The simple and low-cost aptasensor is promisingly applicable for CEA detection in cancer diagnosis, and could be able to serve as a general platform for detection of other interested glycoproteins.
研究领域:
生物传感器
·
电化学
·
癌症诊断
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xueqin Huang,Zhubao Zhang,Lingzhi Chen,Yongjian Lin,Runmin Zeng,Jun Xu,Shanze Chen,Jianglin Zhang,Huaihong Cai,Haibo Zhou,Pinghua Sun
Ultra-sensitive detection of pathogenic bacteria is of great significance in the early stage of bacterial infections and treatment. In this work, we report a novel strategy using multifunctional Au nano-bridged nanogap nanoparticles (Au NNPs)-based sandwich nanocomposites , that made of Concanavalin A-conjugated Fe 3 O 4 @SiO 2 NPs (ConA-Fe 3 O 4 @SiO 2 NPs)/bacteria/aptamer-modified Au NNPs (apt-Au NNPs), for bacteria discrimination and quantitative detection by surface-enhanced Raman scattering (SERS) and inductively coupled plasma mass spectrometry (ICP-MS), and subsequently photothermal antibacterial assay. The sandwich nanocomposite consists of ConA-Fe 3 O 4 @SiO 2 NPs to magnetically enrich and photothermal killing bacteria , and dual-signal tags of apt-Au NNPs for both SERS sensing and ICP-MS quantification. This strategy can specifically distinguish different kinds of pathogenic bacteria, and provided a good linear relationship of Staphylococcus aureus ( S. aureus ) in the range from 50 to 10 4 CFU/mL with a detection limit of 11 CFU/mL, as well as realized ultralow amounts of bacterial detection in serum sample with high accuracy. Based on the quantitative detection, high antibacterial efficiency was monitored by ICP-MS. Overall, the established method combines bacteria discrimination, quantitative detection, and photothermal elimination with a simple and rapid process, which provides a novel way for the early diagnosis and treatment of bacterial infection.
研究领域:
纳米技术
·
分析化学
·
生物医学工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）