Nano Research
·
2023
·
IF 9.9
Yang Limin,Qu Linjiao,Zhang Xiaolong,Li Mingming,Liu Zhen
Enzyme-inorganic hybrid nanoflowers (HNFs) have shown excellent sensing capabilities due to their large specific surface area as well as the simplicity and mildness of the preparation process. However, coupling HNFs to electrodes to fabricate a uniform and controllable enzymatic electrochemical sensing interface remains a challenge. Here, we proposed an aptamer-induced in-situ fabrication strategy for preparing an HNF-based electrochemical sensor with ideal performance. Central to this strategy is the introduction of acetylcholinesterase (AChE)-specific binding aptamer (Apt), which induces the in-situ growth of AChE-copper phosphate (Cu 3 (PO 4 ) 2 ) HNFs on the surface of carbon paper (CP). In addition, a dense gold nanoparticle (AuNP) layer was electrodeposited on the CP for anchoring Apt and further extending the electroactive surface area. The prepared AChE-Cu 3 (PO 4 ) 2 HNF/Apt/AuNP/CP biosensor exhibited a wide detection range from 1 to 10 7 pM for the four organophosphorus inhibitors, including isocarbophos, dichlorvos, methamidophos, and parathion, with detection limits down to 0.016, 0.028, 0.071, and 0.113 pM, respectively. With the reactivation of pralidoxime chloride, the electrode can still recover 98.1% of the response after five times of repeated use. In real sample detection, the biosensor achieved high recoveries from 96.45% to 100.13%. The detection target may be extendable to other AChE inhibitors (e.g., drugs for Alzheimer’s disease). This study demonstrates for the first time the feasibility of using aptamers as an inducer to fabricate an electrochemical enzyme sensing interface in-situ . This strategy can be used to fabricate other enzyme-based biosensors and therefore has broader applications.
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高影响因子
Food Bioscience
·
2024
·
IF 4.8
Hua-Wei Zhang,Ke Cheng,Jing-Jing Tang,Ling-Zhi Liu,Ruo-Xuan Qin,Yi-Nuo Sun,Jiang-Hua Zhang,Da-Yong Zhou
To synthesize structured phospholipids (SPLs) in an efficient and green manner is of great significance. Herein, novel Fe 3 O 4 @SiO 2 - Aspergillus oryzae lipase (AOL)/copper phosphate trihydrate nanoflowers (FLCNs) were prepared by coprecipitation method and then characterized by energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer etc. FLCN activity was further evaluated by promoting functional medium-chain SPL (MCSPL) or short-chain SPL (SCSPL) synthesis via biotransesterification between soybean lecithins and medium-chain fatty acids (MCFAs) or short-chain fatty acids (SCFAs) under optimal reaction conditions. Moreover, FLCNs and free AOLs were comparatively assayed in enzymatic properties. As a result, FLCNs were successfully fabricated with sufficiently large surfaces and numerous enzyme active sites. Owing to these structural features, after reacting at 40 °C for 36 h with FLCN dosage of 6% and substrate mass ratio of 1:3:3, FLCNs exhibited much more excellent activity in MCSPL synthesis with 88.30 ± 0.63% of MCFA incorporation than benchmark enzymes and heterogeneous functional materials. Most significantly, in the first biosynthesis of functional SCSPLs facilitated by FLCNs, much superior SCFA incorporation of 66.55 ± 0.73% was obtained. Notably, FLCNs also possessed convenient magnetic separability together with superior pH and temperature tolerances, thermal and storage stabilities, and recyclability to free AOLs. In brief, the present investigation provides a new biological strategy for effective, economic, convenient, and eco-friendly SPL synthesis in food and pharmaceutical industries, highlighting its merits in functional lipid production.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Jiadong Chen,Xiao Song,Yinna Wu,Junwei Zhu,Wenbin Liu
BSA@Cu 3 (PO 4 ) 2 hybrid nanoflower as nanozyme with tyrosinase mimic activity. • Salt hydrate Na 2 CO 3 ·10H 2 O as essential cofactor for catalysis in organic solvent. • High catalytic performance for oxidation diphenol to o-quinone. • N Cu coordination as nucleation sites for HNF and active sites tyrosinase mimetics. • Production of melanin-like amino acid surfactants from ethyl caffeate. Amino acid surfactants are widely used in daily life and food industry. Melanin-like amino acid surfactant (MLAAS) is a novel type surfactant. Herein, a robust nanozyme with tyrosinase mimetic activity was first developed by fabricating BSA@Cu 3 (PO 4 ) 2 organic-inorganic hybrid nanoflower (HNF) using bovine serum albumin and copper ions as an organic and inorganic components, respectively. HNF nanozyme biomimetically oxidized ethyl caffeate to o-quinone, achieving yield of 59.57% in organic solvents with Na 2 CO 3 ·10H 2 O as essential cofactor. Michaelis constant was 15.05 mM and turnover number was 6.32 s −1 . It exhibited broad substrate scope toward diphenols and outstanding stability in various organic solvents. o-Quinone was conjugated with leucine to produce MLAAS, which possessed excellent emulsifying and foaming capacities with a critical micelle concentration of 8.12 mM. It presents a novel strategy by combination of HNF and salt hydrate for production of amino acid surfactant with promising emulsification and foaming potential in food processing. Download: Download high-res image (144KB) Download: Download full-size image
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 10
·1-10
·
IF 5.7
Wang Na,Yang Juan,Luo Zhi,Qin Dongmiao,Wu Yusheng,Deng Biyang
Copper phosphate hybrid nanoflowers (Cu 3 (PO 4 ) 2 HNFs) were demonstrated to produce cathodic ECL emission in the presence of potassium persulfate (K 2 S 2 O 8 ) and then used as a carrier due to their large specific surface area. AgNPs modified on Cu 3 (PO 4 ) 2 HNFs provided more binding sites for immobilizing secondary antibodies and accelerating the electron transfer rate to enhance the ECL signal. In addition, FONDs-Au was used to capture primary antibodies due to its good biocompatibility and large specific surface area. A sandwich electrochemiluminescence (ECL) immunosensor based on copper phosphate hybrid nanoflower/Ag nanoparticle (Cu 3 (PO 4 ) 2 HNFs@Ag) composite and Au NPs-functionalized Fe 2 O 3 nanodendrites (FONDs-Au) was constructed to detect prostate-specific antigen (PSA) in real samples. Under optimal conditions, the constructed sandwich ECL immunosensor was sensitive to PSA with a detection limit of 0.037 pg/mL ( S / N = 3), a linear detection concentration range of 0.0001–50 ng/mL, and a recovery range of 97.33–102.5%. This immunosensor is expected to provide a method to detect PSA or other biomarkers. Graphical Abstract
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ACS Sustainable Chemistry & Engineering
·
2017
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IF 5.951
Jian Rong,Tao Zhang,Fengxian Qiu,Yao Zhu
Issues with separation and recycling of suspension powder samples in wastewater hinder the practical application of hybrid enzyme materials for environmental pollution control. In this work, for the first time, laccase–Cu3(PO4)2 hybrid microspheres with hierarchical structure were successfully prepared and loaded on a treated copper foil surface. First, Cu8(PO3OH)2(PO4)4·7H2O nanoflowers (CPN) were synthesized on the copper foil surface by a solution-growth method. Then the laccase–Cu3(PO4)2 hybrid microspheres were loaded on the CPN surface via an immersion reaction method using a laccase-containing phosphate buffer solution (PBS) solution. The formation mechanisms of CPN and La–CPN (laccase–Cu3(PO4)2 hybrid microspheres on the CPN surface) are discussed in detail and mainly contain the following processes: crystal growth, coordination effect, in situ growth, and self-assembly. Compared with free laccase, the as-obtained La–CPN has a higher decoloration efficiency (more than 95%) and decoloration rate (nearly 3.6 times higher than that of free laccase) on Congo red dye (CR) solution in the short time of 3 h. Cyclic voltammetry results demonstrated that the oxidizability of immobilized laccase could be enhanced as a result of the presence of Cu2+. Meanwhile, the utilization of the CPN carrier and the unique nanostructure gave the laccase–Cu3(PO4)2 hybrid microspheres high and stable decoloration efficiency and improved the tolerance toward pH and temperature changes. La–CPN still maintained about 85% relative activity after storage for 10 days. The concept presented herein can be further expanded to the preparation of other hybrid enzyme materials for environmental control, medical treatment, and more applications.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Jingling Gong,Bin Xiang,Ruirui Jin,Zhaoyang Li,Weimin Liu,Jian Li
Membrane fouling is a key challenge to the sustainable operation of oil–water separation membranes. Constructing a stable hydration layer on the membrane surface as an oil-fouling resistance barrier by modulating the physicochemical structure of the membrane is one of the most effective strategies. Herein, inspired by the petal structure in nature, Cu 3 (PO 4 ) 2 and UiO-66-NH 2 particles were combined by a self-assembly strategy to construct CUP (Cu 3 (PO 4 ) 2 /UiO-66-NH 2 /PVA) composite membranes with anti-fouling capability against viscous crude oil. Benefiting from the petal structure and abundant polar groups within the membrane, the as-prepared membrane could easily combine with water molecules to form a stable hydration layer, which acted as a barrier to hinder the oil contamination of the membrane. Further, the hierarchical flower-like structure was conducive to the improvement of the emulsion breaking rate and permeation flux of oil-in-water emulsions. After 60 min continuous cross-flow separation, the obtained CUP composite membrane still remained prominent separation efficiency (99.6 %) and permeate flux (4685.96 LMH/bar). In addition, the CUP composite membranes showed outstanding resistance to acid and alkali as well as abrasion. This study provides an effective method for the advancement of high-performance membranes with high flux and anti-fouling capability in the flied of oily wastewater treatment.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Chunyun Ma,Yan Zhang,Chuankai Yang,Yuhang Zhang,Miaorong Zhang,Jianguo Tang
Cetyl trimethyl ammonium bromide (CTAB)-activated lipase-mineral hybrid microflowers (CTAB-Lip-MHFs) were fabricated via biomineralization using Cu 3 (PO 4 ) 2 ⋅3H 2 O as the mineral for olive oil hydrolysis. FT-IR spectra indicated that immobilized lipase molecules in CTAB-Lip-MHFs has good structural integrity, and XPS spectra revealed that immobilized lipase molecules were present at the surface of CTAB-Lip-MHFs. Kinetic studies suggested that CTAB-Lip-MHFs had good substrate affinity ascribed to interfacial effect caused by CTAB, meanwhile, the open conformation of lipase activated by CTAB contributed to increased reaction rate with substrate. The generated fatty acids via hydrolysis of olive oil by CTAB-Lip-MHFs were 146% of that by free lipase, moreover, CTAB-Lip-MHFs could retain 76.5% and 93.2% of initial activities after storage at 50 °C for 10 h and 4 °C for 30 days, respectively, indicating the good stability of CTAB-Lip-MHFs. In addition, after 5 recycles, CTAB-Lip-MHFs preserved 72.7% of initial activity, demonstrating the favorable reusability of CTAB-Lip-MHFs for fatty acid production.
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INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Xu Xu,Xiang Li,Guibin Liu,Xixi Wei,Daming Feng,Lei Zhang
In recent years, efficient oil–water separation has gradually become an indispensable part of environmental treatment. Superhydrophobic/superoleophilic materials with excellent self-cleaning performance are urgently required and remain challenging in the investigation of practical, rapid, and efficient separation of oil–water mixture and emulsion, especially those with robust surfaces that can be used in harsh conditions. In this work, a novel superhydrophobic/superoleophilic material was first fabricated by in situ constructing PDMS@ZIF-7/Cu3(PO4)2 hierarchical architectures on a copper mesh, which was adopted as a high flux and efficient separation material for gravity-driven separation of oil–water mixture as well as emulsion. The introduction of crucial Cu3(PO4)2 nanosheet interlayers created the ideal hierarchical structures and serve as partial templates for the subsequent in situ growth of hydrophobic ZIF-7 nanosheets. An improved superhydrophobicity (CA = 155°), permeation flux (102,000 L m–2 h–1), and preferred self-cleaning property were thus achieved by such manipulation of the copper mesh. The PDMS@ZIF-7/Cu3(PO4)2 mesh exhibited exceptional separation efficiency for diverse oil–water mixtures and emulsions attributed to the superhydrophobicity and the demulsification ability and considerable stability to cope with extreme environments including sunlight resistance, low temperature, and corrosion resistance, which prompted its promising applicability in cleaning emulsified wastewater.
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高影响因子
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