ChemElectroChem
·
2022
·Vol. 9
·期 10
·e202200237
·
IF 4.782
Xiaoxiao Chen,Pu Li,Chen Luo,Chaobiao Huang
Graphical Detection mechanism of PEC sensor for detecting xanthine based on photocurrent signal changes caused by hydrogen peroxide reduction catalyzed by copper nanoclusters A photoelectrochemical (PEC) sensor combining chitosan-coated and glutathione-protected copper nanocluster (CS−GSH−CuNCs) with peroxidase-like activity and xanthine oxidase (XAO) was developed for rapid detection of xanthine. Firstly, CS−GSH−CuNCs was prepared by glutathione (GSH) reduction using chitosan (CS) as protective agent, and then was modified on titanium dioxide nanoparticles (TiO 2 NPs)/ITO electrode which was prepared by sintering titanium dioxide (TiO 2 ) on the ITO electrode surface by high temperature calcination. After excited by light at 350 nm, the photoinduced electrons from the lowest unoccupied molecular orbital (LUMO) of CS−GSH−CuNCs were transferred to the conduction band of TiO 2 , which resulted in spatial separation of electron-hole and enhancement of photocurrent signal. XAO oxidizes xanthine to produce hydrogen peroxide (H 2 O 2 ). Part of the photoinduced electrons from CS−GSH−CuNCs transferred and catalyzed the reduction of H 2 O 2 , resulting in the reduction of the photocurrent. Under optimal conditions, the PEC sensor exhibits good sensitivity and reproducibility with the limit of detection (LOD) of 6.6 nmol ⋅ L −1 and a relative standard deviation of 3.5 % for 10 replicate detections of 1.00 μmol ⋅ L −1 xanthine and the linear range of 0.04–90.0 μmol ⋅ L −1 for xanthine. Furthermore, the PEC sensor presents nice selectivity owing to its enzyme-like activity and was successfully applied to human urine, indicating its great potential for real sample analysis.
研究领域:
生物传感
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分析化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Shengmei Tai,Zhijuan Qian,Hongxin Ren,Alberta Osei Barimah,Chifang Peng,Xinlin Wei
A colorimetric assay based on an enzyme-inhibition strategy is promising for the on-site detection of pesticide residues . However, very few works of pesticide detection were reported based on the inhibition toward nanozymes although nanozymes have demonstrated many advantages in sensing various targets. Herein, a facile colorimetric detection for Glyp was developed based on β [email protected] enzyme mimics. The β [email protected] with high peroxidase-like activity was synthesized using random DNA double strands as template and β -CD as surface ligand. β [email protected] could catalyze the H 2 O 2 -3,3′,5,5′-tetramethylbenzidine (TMB) system. The oxidation product OxTMB with a blue color and presented a large absorption signal at 652 nm. However, Glyp could destroy the synergic effect between redox doublet (Cu 2+ /Cu + ) on the β [email protected] A-CuNCs surface, resulting in the inhibition of the peroxidase-like activity. Colorimetric detection for Glyp could be established by detecting the changes of absorption signal at 652 nm. The linear range was 0.02–2 μ g/mL and the detection limit was 0.85 ng/mL (3δ/s). The method was applied in measuring Glyp spiked in lake water and various food samples. This method had rapidness, high sensitivity, and selectivity advantages, indicating the high application potential in monitoring Glyp residue in food.
研究领域:
分析化学
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环境科学
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食品安全
·
农业化学
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DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2023
·Vol. 17
·期 3
·2850-2857
·
IF 3.4
Yang Xiao-Shuai,Wang Lu-Liang,Zhao Jie,Ji Shi-Lei,Liu Quan-Wen,Peng Xin-Yan,Hu Zhen-Hua,Liu Yu-Shen,Wang Ping
Facile fabrication of copper nanoclusters (CuNCs) with excellent fluorescence is desirable, but remains challenging and scarce for sensing applications in food. In this work, albumin egg (AE) stabilized CuNCs were prepared by a self-assembly templated method. The obtained fluorescent AE-CuNCs exhibited a strong emission at 412 nm upon excitation at 325 nm. Based on the absorption competition quenching mechanism, the AE-CuNCs fluorescent probe for Fe 3+ was developed. The probe had good linearity (0.10–1000 µM) with R 2 (0.986), low limits of detection (30 nM) and good selectivity. Furthermore, the iron content in noodles, biscuits, light soy sauce and bean sprouts was successfully determined by the fluorescent probe. Graphical Abstract
研究领域:
分析化学
·
纳米技术
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食品科学
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DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Bo Yin,Rui Rui Liu,Hong Lin Zhai,Shao Hua Lu,Jia Ying Mi
A simple and facile one-pot approach for the synthesis of copper nanoclusters decorated reduced graphene oxide (CuNCs/RGO) nanocomposite was proposed, in which the CuNCs attached to the surface of the reduced glutathione (GSH) functionalized RGO through ligand exchange via their thiol functionalities. The synthesized nanocomposite was verified by structural characterizations, and the further investigation of density functional theory (DFT) indicated that Cu 3 R 2 cluster (R = C 10 H 16 O 6 N 3 S) with the lowest energy was the most stable structure in GSH-capped CuNCs. Although the CuNCs/RGO nanocomposite exhibited rather weak fluorescence, with the addition of heparin (Hep), the significant enhancement of fluorescence at 595 nm was achieved, which was developed to detect Hep in human serum samples with high selectivity and sensitivity. The mechanisms of fluorescence quenching of CuNCs/RGO nanocomposite and the sensing of Hep were discussed. The linear range was 0.1–10 μM with the detection limit of 26 nM in buffer solution containing 2% human serum sample, and satisfactory recovery in the range of 96.6%–104% was obtained, suggesting that the proposed method could applied to the detection of Hep in human serum samples.
研究领域:
材料科学
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纳米技术
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分析化学
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生物医学工程
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Dan Li,Xiangshan Lei,Qingdong Liu,Yajun Chen,Jiao Wang,Bingyan Han,Gaohong He
Copper nanoclusters (CuNCs), as novel energy level tunable photocatalysts, have been utilized to combine with various conventional inorganic catalysts to improve catalytic performance. However, conventional photocatalysts generally have fixed energy levels, which are not conducive to bi-directional adjustment of the composite catalyst energy level structure. In this work, CuNCs protected by 3-mercaptopropionic acid (CuNCs@3-MPA) and energy-level controllable carbon dots (CDs) are combined to prepare intelligent photocatalytic materials through electrostatic self-assembly strategy, i.e., CuNCs@3-MPA/CDs. The self-assembled CuNCs@3-MPA have a large specific surface area, and the Z-type heterostructure can be constructed by introducing energy-matched CDs, which can achieve the effective separation of photogenerated electron-hole pairs and directional generation of ·O 2 − at the same time, enabling the degradation of 98.5 % Methyl Orange (MO, 20 mg/L) in 7 min. The catalytic rate constant is increased to 0.501 min −1 , which is equivalent to 3.6 times that of CuNCs@3-MPA alone. Since CuNCs@3-MPA/CDs with rich Cu(I) can perform Fenton-like reaction to catalyze the production of ·OH from H 2 O 2 , we use TMB and H 2 O 2 as substrates to probe its peroxidase-like activity. The K m is 0.18 mM when the substrate is TMB, which is lower than that of horseradish peroxidase (HRP) ( K m = 0.434 mM), indicating that the affinity between CuNCs@3-MPA/CDs and TMB is higher than that of HRP, which has a high peroxidase-like activity.
研究领域:
材料科学
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环境科学
·
生物化学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Xiaoye Wen,Chenjie Pang,Chenxi Dai,Zhefeng,Fan
As novel energy level-tunable photocatalysts, copper nanoclusters (CuNCs) have been used for combining with conventional catalysts to synergize enhanced the catalytic capabilities. However, conventional photocatalysts have their own drawbacks. In particular, their fixed energy levels are unfavorable for regulating the energy level structure of composite catalysts. In this study, a novel nanoplatform (CuNCs@CS/MOF) was constructed for the detection and photocatalytic degradation of MBT. The chitosan-modified CuNCs were encapsulated within the MOF matrix. The CuNCs maintained an aggregated state and the rotation of their ligands was restricted, thereby further enhancing the photoluminescence intensity and photostability of CuNCs. When exposed to light irradiation, CuNCs@CS/MOF showed remarkable photocatalytic activity, and the underlying mechanism governing the photocatalytic degradation process was elaborated in detail. Specifically, CuNCs@CS/MOF generated photogenerated electrons and holes, the photogenerated electrons reacted with H 2 O 2 to produce ·OH, while the remaining holes accepted electrons donated by MBT. The generated ROS oxidized TMB to oxidized TMB (oxTMB) under light irradiation, which was thereafter diminished by MBT. On this basis, the quantitative detection of MBT was established. Furthermore, ROS could oxidize and decompose MBT into small-molecule fragments, thus achieving the efficient elimination of MBT. This work provides an attractive strategy for designing multifunctional novel nanoplatform, which can be applied for the accurate detection and further harmless treatment of pollutants.
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PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.6
Lian-Lian Duan,Wen-Jun Liu,Rui Zhai,Zhen-Guang Wang,Hong-Yuan Yan,Yun-Kai Lv
This study employed a pore-confined synthesis strategy to achieve the in situ growth of bovine serum albumin-capped copper nanoclusters (BSA-CuNCs) within the UiO-66 framework (BSA-CuNCs@UiO-66). This nanocomposite enables highly sensitive and specific detection of creatinine (CR). Results demonstrated that the spatial confinement imposed by UiO-66 induced aggregation of the BSA-CuNCs and suppressed non-radiative transitions, leading to an approximately 10-fold enhancement in fluorescence intensity and a 11-fold increase in quantum yield. Leveraging the specific adsorption and enrichment capability of the UiO-66 framework toward CR, the BSA-CuNCs@UiO-66 fluorescence probe exhibited significant fluorescence quenching upon exposure to CR, achieving a detection range of 50–1000 nM and a detection limit of 30.81 nM. This work presented a novel confinement engineering strategy utilizing metal-organic frameworks (MOFs), establishing a new design paradigm for high-performance fluorescence probes with significant potential in bioanalytical applications.
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PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yujiao Cui,Xiaofei Li,Qingyan Gu,Zhixiang Xu,Longhua Xu
The residual presence of ciprofloxacin (CIP) in aquatic products raises, increasing concerns regarding bioaccumulation and antimicrobial resistance, necessitating the development of highly sensitive and selective detection methods. Here, we present a novel ratiometric fluorescence sensor based on bovine serum albumin-stabilized copper nanoclusters (BSA-CuNCs) and Al 3+ for CIP detection. CIP complexes with Al 3+ to simultaneously quench the orange-red emission of BSA-CuNCs at 600 nm and enhance its intrinsic blue emission at 490 nm, thereby producing a self-calibrated ratiometric signal (F 490 /F 600 ) with a clear concentration-dependent response and an observable color transitions from orange-red to blue. Benefiting from macromolecular surface modification of CuNCs and the ratiometric sensing strategy, the sensor displays good stability, high selectivity, and strong anti-interference capacity. It achieves a wide linear dynamic range of 0.025–50.0 mg L −1 (R 2 = 0.9935), a low limit of detection (0.01 mg L −1 ), and practical potential in complex matrices. Validation in diverse aquatic food samples has yielded satisfactory recoveries of 87.0%–108.8% with RSD < 2.4%. To further expand its practical applicability, a smartphone-assisted sensing platform has been developed, enabling accurate RGB-based quantification (R 2 = 0.9929). Statistical comparison with HPLC using paired t -test showed no significant difference between the two methods ( p > 0.05), indicating high analytical concordance. This work establishes a reliable and versatile approach for accurate quantification and intuitive visual screening of CIP residues, and also suggests the potential for future extension toward simultaneous detection of Al 3+ and CIP in real-world applications.
研究领域:
分析化学
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食品安全
·
纳米生物技术
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药物残留检测
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环境监测
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荧光传感
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转化医学
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DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 2
·638-642
·
IF 1.938
Wei Wang,Lei Zhan,Yu Qing Du,Fei Leng,Cheng Zhi Huang
A novel and selective spectrofluorometric method for pyrophosphate (PPi) assay in aqueous solution was developed in this contribution on the basis of the enhanced fluorescence intensity of trypsin-stabilized copper clusters (CuNCs). Mechanism investigation showed that PPi can make the CuNCs dispersed, concomitantly resulting in the improved quantum yield and the enhanced fluorescence emission of the CuNCs. A good linear relationship in the range of 78 μM–20 mM was achieved between the enhanced fluorescence intensity of the CuNCs at 455 nm and the logarithm of PPi concentration with the detection limit (3σ) of 101 nM. By this approach, we can achieve simple, fast and inexpensive detection of PPi.
研究领域:
分析化学
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光谱学
·
纳米材料
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DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Fubin Yang,Songrui Li,Jiutong Ma,Qiong Jia
The excessive use of chlorpyrifos (CPF) has caused serious harm to food and soil safety, so, it is important for the detection of CPF. Herein, a fluorescence sensor (aZIF-8@CuNCs) consisting of copper nanoclusters (CuNCs) wrapped by amorphous zeolite imidazole framework-8 (aZIF-8) was prepared by a facile one-pot strategy to achieve selective detection of CPF. The sensing mechanism is that the fluorescence of aZIF-8@CuNCs can be quenched by 3,5,6-trichloro-2-pyridinol, the hydrolysate of CPF in alkaline condition through the inner filter effect. Therefore, a specific enzyme-free CPF sensing method was constructed by using aZIF-8@CuNCs. The detection range is 0–50 μg/mL and the detection limit reaches 0.43 μg/mL, and the recovery range of CPF in food and soil is 90.6 %-112.9 % with the relative standard deviations less than 6 %. In addition, aZIF-8@CuNCs were successfully combined with a smartphone to realize rapid visualization and quantitative analysis of CPF. This method not only reduces the difficulty of MOF@CuNCs synthesis, but also opens up a new avenue to apply it to pesticide detection in food and soil.
研究领域:
分析化学
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食品安全
·
环境科学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Guansong Hu,Junjian Chen,Yan Fan,Haiyan Zhou,Kunzhong Guo,Zhou Fang,Liangxu Xie,Lin Wang,Yingjun Wang
As an effective strategy to develop novel implants with a biomolecule, the efficiency of a copper-catalysed azide-alkyne cycloaddition (CuAAC) urgently requires improvement. Herein, we constructed a facile CuAAC system with catalytic CuSO 4 /sodium borohydride (CuAAC-Bor). As Cu nanoclusters (CuNCs) formed in situ , they showed stronger efficiency in immobilizing 41 types of peptides on Ti implants than traditional CuAAC with CuSO 4 /sodium ascorbate. Then, we mechanically revealed the significance of peptide size on CuAAC efficiency by all-atom molecular dynamics simulation and machine learning, and found that larger peptides preferred to adhere to Ti and CuNCs to increase their reaction opportunity. With CuAAC-Bor, HHC36-engineered implants strongly inhibited 5 types of clinical bacteria in vitro and prevented infection in vivo , and RGD-, DGEA-, QK- or YGFGG-engineered implants had high biocompatibilities with HUVECs / hBMSCs in vitro . Moreover, we demonstrated that QK-engineered implants prepared with CuAAC-Bor promoted the osteogenic differentiation of hBMSCs via the PI3K/Akt signalling pathway and triggered angiogenesis and osteogenesis in vivo . Our study shows that this facile and highly efficient CuAAC-Bor system holds high promise for the preparation of novel biomaterial surfaces.
研究领域:
生物材料
·
生物医学工程
·
分子生物学
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）