CANCER LETTERS
·
2018
·
IF 6.491
Yingming Sun,Hongtao Miao,Shijing Ma,Lei Zhang,Chengcheng You,Fang Tang,Cui Yang,Xiaoli Tian,Feng Wang,Yuan Luo,Xiangjie Lin,Hui Wang,Chunyang Li,Zhijun Li,Hongnv Yu,Xuefeng Liu,Yu Xiao,Yan Gong,Junhong Zhang,Hong Quan,Conghua Xie
FePt-Cys nanoparticles (FePt-Cys NPs) have been well used in many fields, despite their poor solubility and stability. We synthetized a cysteine surface modified FePt NPs, which exhibited good solubility, stability and biocompatibility. We explored the insight mechanisms of the antitumor effects of this new nanoparticle system in lung cancer cells. In the in vitro study, FePt-Cys NPs induced a reactive oxygen species (ROS) burst, which suppressed the antioxidant protein expression and induced cell apoptosis. Furthermore, FePt-Cys NPs prevented the migration and invasion of H1975 and A549 cells. These changes were correlated with a dramatic decrease in MMP-2/9 expression and enhanced the cellular attachment. We demonstrated that FePt-Cys NPs promoted the effects of chemo-radiation through activation of the caspase system and impairment of DNA damage repair. In the in vivo study, no severe allergies or drug-related deaths were observed and FePt-Cys NPs showed a synergistic effect with cisplatin and radiation. In conclusion, with good safety and efficacy, FePt-Cys NPs could therefore be potential sensitizers for chemoradiotherapy.
研究领域:
纳米医学
·
肿瘤学
·
药理学
·
放射生物学
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DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2017
·Vol. 46
·期 7
·2204-2213
·
IF 4.029
Lanfang Ren,Xijian Liu,Qian Wang,Lijuan Zhang,Guoying Deng,Feng Zhou,Jie Lu
In this study, a multifunctional nano-drug (PTX-loaded Cys-Fe3O4/CuS@BSA nanocomposite), which performs well in hydrophobic drug delivery, magnetic resonance imaging (MRI) and chemo-photothermal combination therapy, has been successfully synthesized via a facile method with several biocompatible agents. The characterization verified that our as-obtained nanocarrier has a preferable average diameter of about 300 nm for cancer therapy, shows a great MRI ability and magnetism performance, and exhibits a favorable in vitro and in vivo photothermal therapeutic efficacy by introducing CuS, and the sustained release of chemotherapeutics could be controlled by pH value and near-infrared laser irradiation has further enhanced the therapeutic performance. Overall, the excellent synergistic effect to inhibit tumor growth effectively realized via imaging-guided combinational chemo-photothermal therapy in the present work, highlights the promise of conquering the limitations of tumor treatments.
研究领域:
生物医学工程
·
癌症治疗
·
纳米技术
·
分子影像
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Min Liao,Minghao Jiang,Xumiao Wang,Wei Hu,Ning Zhao,Haiqun Cao
Acetyl-CoA carboxylase (ACCase)-inhibiting herbicides are among the most commonly used herbicides to control grassy weeds, especially Leptochloa chinensis , in rice fields across China. Herein, we collected a suspected resistant (R) population of L. chinensis (HFLJ16) from Lujiang county in Anhui Province. Whole plant dose response tests showed that, compared with the susceptible (S) population, the R population showed high resistance to cyhalofop-butyl (22-fold) and displayed cross-resistance to metamifop (9.7-fold), fenoxaprop- P -ethyl (18.7-fold), quizalofop- P -ethyl (7.6-fold), clodinafop-propargyl (12-fold) and clethodim (8.4-fold). We detected an amino acid substitution (Cys-2088-Arg) in the ACCase of resistant L. chinensis . However, ACCase gene expression levels were not significantly different ( P > 0.05) between R plants and S plants, without or with cyhalofop-butyl treatment. Furthermore, pretreatment with piperonyl butoxide (PBO, a cytochrome P450 monooxygenase (CYP450) inhibitor) or 4-chloro-7-nitrobenzoxadiazole (NBD-Cl, a glutathione-S-transferase (GST) inhibitor), inhibited the resistance of the R population to cyhalofop-butyl significantly (by approximately 60% and 26%, respectively). Liquid chromatography tandem mass spectrometry analysis showed that R plants metabolized cyhalofop-butyl and cyhalofop acid (its metabolite) significantly faster than S plants. Three CYP450 genes, one GST gene, and two ABC transporter genes were induced by cyhalofop-butyl and were overexpressed in the R population. Overall, GST-associated detoxification, CYP450 enhancement, and target-site gene mutation are responsible for the resistance of L. chinensis to cyhalofop-butyl.
研究领域:
植物科学
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分子生物学
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杂草科学
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除草剂抗性
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Sijia Wu,Ping Jiang,Xiaoliang Zhang,Chen Mao,Yaxi Dai,Hong Zhuang,Yong Pang
Understanding the transport mechanism of the peptide Asn-Cys-Trp (NCW) is crucial to improving its intestinal absorption and bioavailability. This study investigated the absorption of NCW through Caco-2 cell monolayers and its interaction with the DPPC bilayers. Results revealed that after a 3 h incubation, the Papp (AP-BL) and Papp (BL-AP) values of NCW at a concentration of 5 mmol/L were (22.24 ± 4.52) × 10–7 and (6.63 ± 2.31) × 10–7 cm/s, respectively, with the transport rates of 1.59 ± 0.32 and 0.62 ± 0.20%, indicating its moderate absorption. NCW was found to be transported via PepT1 and paracellular transport pathways, as evidenced by the significant impact of Gly-Pro and cytochalasin D on the Papp values. Moreover, NCW upregulated ZO-1 mRNA expression. Further investigation of the ZO-1-mediated interaction between NCW and tight junction proteins will contribute to a better understanding of the paracellular transport mechanism of NCW. The interaction between NCW and the DPPC bilayers was predominantly driven by entropy. NCW permeated the bilayers through electrostatic, hydrogen bonding, and hydrophobic interactions, resulting in increased fluidity, flexibility, and disorder as well as phase transition and phase separation of the bilayers.
研究领域:
药理学
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生物物理学
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细胞生物学
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膜科学
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2023
·
IF 10
Xianbao Sun,Lingling Xu,Hai-Dong Xu,Limin Xie,Rui Wang,Zhimou Yang,Wenjun Zhan,Shurong Shen,Gaolin Liang
Current molecular photoacoustic (PA) probes are designed with either stimulus-turned “on” or assembly-enhanced signals to trace biological analytes/events. PA probes based on the nature-derived click reaction between 2-cyano-6-aminobenzothiazole (CBT) and cysteine (Cys) (i.e., CBT-Cys click reaction) possess both “turn-on” and “enhanced” PA signals; and thus, should have higher sensitivity. Nevertheless, such PA probes, particularly those for sensitive imaging of tumor hypoxia, remain scarce. Herein, a PA probe NI-Cys(StBu)-Dap(IR780)-CBT (NI-C-CBT) is rationally designed, which after being internalized by hypoxic tumor cells, is cleaved by nitroreductase under the reduction condition to yield cyclic dimer C-CBT-Dimer to turn the PA signal “ON” and subsequently assembled into nanoparticles C-CBT-NPs with additionally enhanced PA signal (“Enhanced”). NI-C-CBT exhibits 1.7-fold “ON” and 3.2-fold overall “Enhanced” PA signals in vitro. Moreover, it provides 1.9-fold and 2.8-fold overall enhanced PA signals for tumor hypoxia imaging in HeLa cells and HeLa tumor-bearing mice, respectively. This strategy is expected to be widely applied to design more “smart” PA probes for sensitive imaging of important biological events in vivo in near future.
研究领域:
生物医学工程
·
分子影像学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
LUMINESCENCE
·
2026
·Vol. 41
·期 3
·e70462
·
IF 2.8
Rui Tian,Yuhui Yang,Duo Li,Jiamin Chai,Rui Zhang,Hua Yang,Haoyu Li,Xuehua Sun
Polyvinylpyrrolidone (PVP) and cysteine (Cys) double-ligand–modified copper nanoclusters (PVP-Cys CuNCs) were synthesized by a one-pot method. The addition of Cys can protect copper nanoclusters (CuNCs) more effectively and enhance its fluorescence intensity, compared with the single-ligand-modified PVP CuNCs; the double-ligand-modified PVP-Cys CuNCs showed higher fluorescence intensity and sensing ability. Based on the internal filtration and dynamic quenching effect of berberine (BBR) on PVP-Cys CuNCs, a novel, simple, and sensitive method for BBR detection has been established. This method exhibits a good linear response to BBR within the linear range of 1.09 × 10 −7 –4.26 × 10 −6 mol · L −1 , and with a limit of detection (LOD) of 3.67 × 10 −8 mol · L −1 . It has been successfully applied to the determination of BBR in practical pharmaceutical samples.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2021
·Vol. 56
·期 22
·12803-12813
·
IF 4.22
Ali M. R.,Bacchu M. S.,Al-Mamun M. R.,Rahman M. M.,Ahommed M. S.,Aly M. Aly Saad,Khan M. Z. H.
An electrochemical sensor based on molecular imprinted polymer (MIP) to detect ceftizoxime (CFX) with high sensitivity and selectivity is demonstrated. MIP was synthesized by electropolymerization of poly-cysteine (P-Cys) on a multi-walled carbon nanotube (MWCNT) modified glassy carbon electrode (GCE). A targeted drug was used as a template molecule during the polymerization process. The bare GCE was coated with a layer of MWCNT before the synthesis of MIP to improve the sensor sensitivity. Experimental parameters such as polymerization conditions, the influence of pH, molar ratio of the template molecules and the monomer molecules were all optimized. The peak potential exhibited linearity with CFX concentration in the ranges of 1 \(\times 10^{ - 9}\) –1× \(10^{ - 7}\) molL −1 ( R 2 = 0.9904) and 2× \(10^{ - 7}\) –1× \(10^{ - 4}\) molL −1 ( R 2 = 0.9949). The LOD of the MIP sensor was found to be \(1 \times 10^{ - 10}\) molL −1 under optimal conditions using a differential pulse voltammetry (DPV). The proposed sensor was tested on real samples, and good recovery results were obtained.
研究领域:
分析化学
·
药物分析
·
生物传感技术
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产品关联
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2021
·
IF 4.291
Mengyang Lu,Qi Zhang,Zhicheng Zhang,Xueqi Wang,Bing Yan,Yongqian Wang
There are a variety of biological ion channels in organisms, which change under the influence of the environment and regulate the material transport at both ends of the biofilm. Inspired by this, a large number of studies on biomimetic nanochannels have been carried out. The lack of selective specificity and detection sensitivity are considered as main drawbacks in biomimetic nanochannels, and the combination of nanomaterials and biomimetic nanochannels is one of solutions. In this research, a confined self-assembly system composed of anodic aluminum oxide (AAO) templates and ZnS nanoparticles was fabricated by a negative pressure filtration method. Besides, L- Cysteine ( L- Cys) and D- Cysteine ( D- Cys) were modified into the confined self-assembly system by ultraviolet irradiation to enhance the sensitivity for biological molecules response. The confined self-assembly system showed specific selection of L- Cys and high sensitivity to biological molecules response. This work not only synthesized ZnS nanoparticles in biomimetic nanochannels controllably but also provided a new way of studying how to utilize nanomaterials to improve the response sensitivity of biomolecules in biomimetic nanochannels.
研究领域:
纳米技术
·
仿生学
·
材料科学
·
生物传感
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Yuxiu Fan,Dong Li,Xiaoyi Xie,Yi Zhang,Ling Jiang,Bin Huang,Xiupei Yang
It is vital to create a sensitive and trustworthy sensor for aflatoxin B1 (AFB1) considering the potential hazards this substance poses to the safety of food. Herein, a colorimetric sensor, namely, aptamer-modified flower-like L-cysteine-functionalized FeNi bimetallic nanoparticles (L-Cys-FeNiNPs) with peroxidase-mimicking enzymatic activity, was established for the detection of aflatoxin B1. Interestingly, based on the synergies between FeNi bimetals and the protection and regulation of morphology by L-Cys on FeNiNPs, we successfully synthesized flower-like L-Cys-FeNiNPs, which were employed as outstanding peroxidase-mimic enzymes to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . Kinetic analysis showed that the flower-like L-Cys-FeNiNPs have an ultralow Michaelis constant ( K m ) and an ultrahigh affinity for H 2 O 2 . Furthermore, based on the peroxidase-mimicking activity of flower-like L-Cys-FeNiNPs, a new colorimetric aptamer sensing strategy was employed for the easy and specific detection of AFB1 with a wide response range (0.12–2 μg/mL) and a low detection limit (36.57 ng/mL). Finally, to verify the practicality of this aptamer detection strategy, we successfully applied it to the detection of AFB1 in actual millet and maize samples.
研究领域:
食品安全
·
生物传感
·
纳米材料
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Juan Liu,Zhi-Qing Wang,Guo-Jiang Mao,Wen-Li Jiang,Min Tan,Fen Xu,Chun-Yan Li
Cysteine (Cys), a kind of small molecule biological thiol, not only involves in the regulation of physiological processes , but also is considered a marker of tumor. However, it is challenging to develop suitable probe for detecting Cys in tumors. In this paper, a near-infrared (NIR) fluorescent probe named IX for Cys has been designed and synthesized. The probe shows a NIR emission peak at 770 nm with large Stokes shift (180 nm) upon adding Cys. It displays high sensitivity to Cys with 6-fold increase of fluorescence intensity . Meanwhile, IX has the high selectivity to Cys over other potential interference such as Hcy and GSH, which have similar structure with Cys. In addition, a possible mechanism of fluorescence enhancement is the reaction of IX with Cys to release IX-OH, which is verified by fluorescence spectra , MS and HPLC. Next, IX can selectively image Cys in HCT-116 cells thanks to the low cytotoxicity. Most important of all, the fluorescent probe IX has visualized Cys in HCT116-xenograft tumor mice due to the near-infrared properties with large Stokes shift.
研究领域:
生物化学
·
医学影像
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
ORGANOMETALLICS
·
2013
·
IF 4.145
Huili Chen,Xiaokai Li,Ruichun Bai,Yanbo Wu,Yingfang Fan,Jianbin Chao
A phosphorescent probe, Ir(ppy)2Cl(DMF) (Ir2), for cysteine (Cys) is reported. The recognition is based on the time-dependent emission of Ir2-Cys. Importantly, this probe can discriminate Cys rapidly from homocysteine (Hcy). Probe Ir2 displays a highly selective 72 nm of luminescent red-shift toward Cys within 30 min with an obvious emissive color change from green to orange. After 30 min, the emission band blue shifts gradually with an emissive change from orange to green. TDDFT combined with CV studies indicates that the different emission profile is ascribed to the different binding modes of Ir2 to Cys with time. The probe can be used to determine Cys in semiaqueous solution.
研究领域:
无机化学
·
分析化学
·
生物无机化学
·
发光传感器
·
分子探针
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Shu Chen,Weiwei Yu,Guangnan Xing,Zhiguang Song,Guodong Feng
Cysteine (Cys) is one of the most basic mercaptans in the human body. As an important endogenous small molecule mercaptan, Cys plays a vital role in various physiological processes and can participate in maintaining redox balance to ensure homeostasis. Abnormal Cys levels can lead to a variety of diseases. However, the detection of cysteine may be interfered with by other small molecule biothiols. Therefore, the design of fluorescent probes based on the structural characteristics and reactivity of cysteine has become the focus of current research. In this paper, a fluorescent probe (3-(2H-benzo[d][1,2,3]triazol-2-yl)-2-oxo-2H-benzo[g]chromen-8-yl acrylate, BTAB) for Cys detection was synthesized with acrylic ester as the reaction site. Under the conditions of gradual optimization, BTAB can achieve selectivity and anti-interference ability for Cys detection. The linear range of Cys was 0.3–10 μM, and the detection limit was 0.154 μM. Finally, this probe was applied to detect the Cys content in bovine serum samples with satisfactory results.
研究领域:
生物化学
·
医学检测
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DOI
PubMed
高影响因子
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