INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2024
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IF 3.8
Dongbo Wang,Xuan Zhou,Yanbo Liu,Mingyang Chen,Dandan Han,Junbo Gong
With the rise of research on functional sugar, we found that preparing crystalline powders into spherical crystals will significantly improve quality, stability, appearance, and texture. In this work, d-ribose (D-RIB) spherical crystal aggregates were prepared for the first time. And D-RIB spherical crystal aggregates with superior filterability, flowability, and tap density can be prepared in ethanol, 1-propanol, and isopropanol. Specifically, we used ethanol as an example to reveal the formation mechanism of D-RIB spherical crystal aggregates: solvent-induced crystal defects, which in turn trigger noncrystallographic branching and further spherulite growth. Finally, a comparison of the properties of the D-RIB spherical crystal aggregates with those of commercially available products is given, demonstrating the fabrication of D-RIB spherulites with better tap density and flowability by solvent-induced noncrystallographic branching. This will provide a new insight into the preparation of high-quality D-RIB crystals.
研究领域:
化学
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材料科学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CATALYSIS
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2014
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IF 1.552
Xingcui Guo,Xicheng Wang,Jing Guan,Xiufang Chen,Zhangfeng Qin,Xindong Mu,Mo Xian
Ru particles were introduced into the zeolite ZSM-5 (MFI) by either a conventional impregnation method or a one-step template-free process. The resultant materials were characterized by X-ray diffraction, N 2 adsorption-desorption, scanning electron microscopy, transmission electron microscopy, NH 3 and CO 2 temperature-programmed desorption. The results indicated that the Ru species in ZSM-5 catalysts prepared via the latter approach (designated as Ru/ZSM-5-TF) were highly dispersed in the ZSM-5 framework structure and this material exhibited high catalytic performance during the hydrogenation of D-glucose to D-sorbitol. The conversion of D-glucose was as high as 99.6% with D-sorbitol selectivity reaching 99.2%, exceeding the performance of Ru/ZSM-5 catalysts prepared by the conventional impregnation method with microporous or desilicated ZSM-5 supports. More importantly, this catalyst showed high stability against leaching and poisoning and could be reused several times. The extensive dispersion of the Ru species, strong interaction between the Ru species and the ZSM-5, and the suitable surface acidity-basicity balance of the Ru/ZSM-5-TF were all critical factors leading to excellent catalytic behavior and stability.
研究领域:
化学工程
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催化科学
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DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
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2021
·
IF 3.493
Dong-Xu Jia,Chen-Yi Sun,Yi-Ting Jin,Zhi-Qiang Liu,Yu-Guo Zheng,Mian Li,Hong-Yan Wang,De-Shui Chen
Allulose is an epimer of d -fructose with multiple nutritional functions, which can be biologically synthesized from d -fructose employing d -allulose 3-epimerase (DAE). In this study, NCBI database mining approach was applied to screen novel DAEs, and Novibacillus thermophilus DAE (NtDAE) exhibiting a high activity of 273.9 U/(g⋅wet cell weight) was selected for investigation. NtDAE showed optimum reaction temperature and pH at 70 °C and 7.0, respectively. In the presence of Co 2+ , its activity was improved by 100.1 %, and the half-life ( t 1/2 ) values reached up to 846.3, 539.2 and 47.8 min at 40, 50 and 60 °C, respectively. Higher k cat / K m values toward d -allulose indicated that the preferred physiological substrate of NtDAE was d -allulose. The conversion yield of 500 g/L d -fructose to d -allulose at 60 °C achieved 29.7 %, and further enhanced to 51.6 % with adding 1.1 M borate. This study provided a useful guidance of further engineering and application of NtDAE for realization of industrial production of d -allulose.
研究领域:
生物化学
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食品科学
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工业微生物学
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DOI
PubMed
高影响因子
Gels
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2024
·Vol. 10
·期 12
·808
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IF 5
Feng Li,Ruoxi Yang,Jian Xu,Guohai Xu,Ye Wu
The concentration of small molecules reflects the normality of physiological processes in the human body, making the development of simple and efficient detection equipment essential. In this work, inspired by a facile strategy in point-of-care detection, two devices were fabricated to detect small molecules via photocurrent measurement. A linear response of the photocurrent against the concentration of the small molecules was found. In the first device, metal ions were introduced into gel substrates made by xanthan gum to enhance photocurrent response. N-phenyl-2-naphthylamine was measured when iron or manganese ions were used. L-Phenylalanine was measured when the gel was modified by samarium, iron, cerium, or ytterbium ions. L-(+)-Arabinose was detected via the gels modified by iron or holmium ions. D-(+)-Mannose was detected when the gel was modified by ytterbium, manganese, chromium, or sodium ions. L-Methionine was detected in the gels modified by samarium, zinc, or chromium ions. The second device was based on a paper sheet. A sugar-like molecule was first synthesized, which was then used to modify the paper. The detection was possible since the photocurrent showed a linear trend against the concentration of D-Trehalose. A linear fit was conducted to derive the sensitivity, whose value was found to be 5542.4. This work offers a novel, simple, and environmentally sustainable platform that is potentially useful in remote areas lacking medical devices.
研究领域:
生物医学工程
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分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
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2021
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IF 4.889
Yanan Gao,Liwei Dou,Hong Gao,Jie Song,Shibin Shang,Zhanqian Song
D-π-D hole-transporting materials have the potential to be excellent dopant-free hole-transporting materials (HTMs) for perovskite solar cells (PSCs) because of their tunable energy levels, high charge mobility, good solubility, and excellent film-forming properties. In this paper, two series of D-π-D type compounds, Series 1 (2TPA-CZ1~2TPA-CZ3) and Series 2 (2DTPA-CZ1~2DTPA-CZ3), were synthesized by linking a carbazole derivative (as a π-bridge) to triaryamine or dehydroabietic acid triarylamine groups (the donor groups) via C–C coupling reactions. The optical, electrochemical, thermal, and photovoltaic properties in PSCs of these compounds were fully investigated. The two series of compounds exhibit good ultraviolet absorption, fluorescence emission , and electrochemical and thermal properties. In addition, their highest occupied molecular orbital levels and energy gaps make them suitable for use as hole-transporting materials in PSCs. Among the PSCs developed, Device 1, based on 2TPA-CZ1, showed the best performance, with a power conversion efficiency (PCE) of 12.27%, a open circuit voltage (V OC ) of 1.13 V, a short-circuit photocurrent density (J SC ) of 15.10 mA cm −2 , and a fill factor (FF) of 71.61%, which is comparable to that of a device based on the conventional hole-transporting material Spiro-OMeTAD. While the PCE of Devices 4–6 was lower than the PCE of Devices 1–3, Devices 4–6 exhibit better stability. This may be due to the higher T g and better film-forming properties of compounds formed using the dehydroabietic acid skeleton. In additional, the introduction of alkyl groups on the carbazole moiety decreased the efficiency of the PSC devices. These results demonstrate that the 2TPA-CZ1 compounds are promising HTMs for PSC applications.
研究领域:
材料科学
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能源科学
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有机化学
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产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 3.475
Hong-Yan Wang,Yu-Li Xie,Xin Shi,Hong-Ling Shi,Jian-He Xu,Cun-Duo Tang,Lun-Guang Yao,Yun-Chao Kan
Halogenated mandelate and its derivatives are important scaffolds in chemical industry. However, the natural D -mandelate dehydrogenase (DMDH) showed relatively low catalytic activity toward D -o-chloromandelic acid, which largely limits the efficiency of dehydrogenation processes. To improve catalytic activity of Lactobacillus brevis D -mandelate dehydrogenase ( Lb DMDH) toward halogenated substrate, a D -mandelate dehydrogenase mutant Lb DMDH N253S with higher catalytic activity toward D -o-chloromandelic acid was successfully obtained by directed evolution, which was about 29 times of Lb DMDH. In addition, the temperature optimum of Lb DMDH N253S is 50 °C, and closer to the optimal growth temperature for Escherichia coli , indicating that it may be more advantageous in whole cell catalysis using living cells as catalysts. Meanwhile, the K cat value toward D -o-chloromandelic acid of Lb DMDH N253S is 2.08 s −1 , which is significantly higher than the one (0.09 s −1 ) of Lb DMDH, indicating that Lb DMDH N253S displayed higher catalytic efficiency toward D -o-chloromandelic acid. Then, we preliminarily explained the molecular basis for its catalytic performance by in silico design methods. All of these results established a solid foundation for molecular modification of DMDHs and the other industrial enzymes.
研究领域:
生物化学
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酶工程
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分子生物学
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产品关联
DOI
高影响因子
Materials Today Communications
·
2022
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IF 3.662
Yongliang Liu,Shengbo Zhu,Yilin Su,Ran Chen,Wenzhi Zhang,Xiaoling Niu,Weixing Chen,Xinbing Chen,Zhongwei An
Three new D-π-D thiophene derivatives ( TPAThTPA , TPAThThTPA , and TPATTTPA ) are synthesized and employed to fabricate the solution-typed electrochromic devices . Density functional theory (DFT) calculation, electrochemical and photophysical characterization is carried out to study their electrochromic performances. When the applied voltage is conducted, the UV–vis absorption wavelength does not shift, but the absorbance increases significantly, accompanied by electrochromic behaviors. After removing the applied voltage, the self-bleaching phenomenon occurs. In addition, electrochromic devices based on these three D-π-D thiophene derivative solutions are fabricated by a simple process. In a word, this research provides a feasible method for developing new electrochromic materials and devices.
研究领域:
材料科学
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电化学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
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IF 4.098
Lingyuan Liu,Qianyi Zhang,Feng Li,Mei Wang,Jing Sun,Shuyun Zhu
Herein, fluorescent DNA-templated silver nanoclusters (DNA-AgNCs) with red emission were synthesized and utilized as novel probe to detect D-penicillamine (D-Pen) for the first time. D-Pen molecules contain a thiol which can combine with Ag to form a non-fluorescent ground state complex, inducing the aggregation of DNA-AgNCs followed by the fluorescence quenching. The quenching mechanism is well-studied and found to be a static quenching process. This method can detect D-Pen in the range of 0.025–0.7 μM with the detection limit as low as 8 nM, which is 1–3 orders of magnitude more sensitive than those based on other fluorescent nanoprobes. More importantly, the preparation procedure for DNA-AgNCs is fast and without the requirement of heavy metal ions. Thus, this detection strategy is time-saving and eco-friendly. Satisfactory recoveries have been acquired for monitoring D-Pen in human serum samples and pharmaceutical samples owing to the high sensitivity.
研究领域:
生物化学
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
npj Biofilms and Microbiomes
·
2023
·Vol. 9
·期 1
·1-14
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IF 9.2
Hu Jie,Wu Yifan,Kang Luyuan,Liu Yisi,Ye Hao,Wang Ran,Zhao Jinbiao,Zhang Guolong,Li Xilong,Wang Junjun,Han Dandan
Elimination of specific enteropathogenic microorganisms is critical to gut health. However, the complexity of the gut community makes it challenging to target specific bacterial organisms. Accumulating evidence suggests that various foods can change the abundance of intestinal bacteria by modulating prophage induction. By using pathogenic Escherichia coli ( E. coli ) ATCC 25922 as a model in this research, we explored the potential of dietary modulation of prophage induction and subsequent bacterial survival. Among a panel of sugars tested in vitro, D -xylose was shown to efficiently induce prophages in E. coli ATCC 25922, which depends, in part, upon the production of D -lactic acid. In an enteric mouse model, prophage induction was found to be further enhanced in response to propionic acid. Dietary D -xylose increased the proportion of Clostridia which converted D -lactic acid to propionic acid. Intestinal propionic acid levels were diminished, following either oral gavage with the dehydrogenase gene ( ldhA )-deficient E. coli ATCC 25922 or depletion of intestinal Clostridia by administration of streptomycin. D -Xylose metabolism and exposure to propionic acid triggered E. coli ATCC 25922 SOS response that promoted prophage induction. E. coli ATCC 25922 mutant of RecA, a key component of SOS system, exhibited decreased phage production. These findings suggest the potential of using dietary components that can induce prophages as antimicrobial alternatives for disease control and prevention by targeted elimination of harmful gut bacteria.
研究领域:
微生物学
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胃肠病学
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营养学
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产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2024
·
IF 3.3
Zhi-Qiang Wang,Wanjun Guo,Shengchao Jia,Yaxuan Jing,Shiting Li,Yong Guo,Xiaohui Liu,Xue-Qing Gong,Yanqin Wang
The selective production of primary amines through reductive amination has received increasing attention due to their extensive applications, while efficient reductive amination is still challenging. Herein, a novel strategy was developed to realize the efficient reductive amination of carbonyl compounds with NH3 via d–d orbital coupling strategies in RuCo alloys. The designed series of RuCo alloy catalysts not only exhibited excellent performance in conversion of various substrates but also demonstrated universal applicability across different supports. The key role of RuCo alloy is its unique ability to effectively catalyze the ammonolysis of a Schiff base, the rate-determining step in reductive amination. Theoretical studies reveal that the d–d orbital coupling strategies break the five d orbital equivalent contributions of metal (Co or Ru) atoms, and the d orbitals primarily contribute through the dz2 and dx2-y2 orbitals of Ru and the dz2 orbital of Co on the RuCo(001) surface, which significantly driving the homolytic NH3 dissociation during the ammonolysis of the Schiff base. Furthermore, stable Co–H covalent bond species and RuCo-NH2 half-ionic and half-covalent bond species on the RuCo(001) surface were generated with lower energy levels. This work not only achieves efficient reductive amination but also provides an atomic orbital-level understanding of the d–d orbital coupling strategies for heterogeneous catalysis.
研究领域:
催化化学
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材料科学
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理论化学
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产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2024
·
IF 3.4
Dingyu Guo,Zhengchao Wang,Wanqing Wei,Wei Song,Jing Wu,Jian Wen,Guipeng Hu,Xiaomin Li,Cong Gao,Xiulai Chen,Liming Liu
D-allulose is a naturally occurring rare sugar and beneficial to human health. However, the efficient biosynthesis of D-allulose remains a challenge. Here, we mined a new D-tagatose 3-epimerase from Kroppenstedtia eburnean (KeDt3e) with high catalytic efficiency . Initially, crucial factors contributing to the low conversion of KeDt3e were identified through crystal structure analysis, density functional theory calculations (DFT), and molecular dynamics (MD) simulations. Subsequently, based on the mechanism, combining restructuring the flexible region, proline substitution based onconsensus sequence analysis, introducing disulfide bonds , and grafting properties, and reshaping the active center, the optimal mutant M5 of KeDt3e was obtained with enhanced thermostability and activity. The optimal mutant M5 exhibited an enzyme activity of 130.8 U/mg, representing a 1.2-fold increase; T m value increased from 52.7 °C to 71.2 °C; and half-life at 55 °C extended to 273.7 min, representing a 58.2-fold improvement, and the detailed mechanism of performance improvement was analyzed. Finally, by screening the ribosome-binding site (RBS) of the optimal mutant M5 recombinant bacterium (G01), the engineered strain G08 with higher expression levels was obtained. The engineered strain G08 catalyzed 500 g/L D-fructose to produce 172.4 g/L D-allulose, with a conversion of 34.4% in 0.5 h and productivity of 344.8 g/L/h on a 1 L scale. This study presents a promising approach for industrial-scale production of D-allulose.
研究领域:
生物化学
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食品科学
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酶工程
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Jing-Jing Liu,Zhan-Yi Wang,Bin-Bin Jiang,Shu-Qin Gao,Ying-Wu Lin
Nonenzymatic glycation and the subsequent accumulation of advanced glycation end-products (AGEs) in proteins are factors underlying long-term pathogenesis in diabetes. The study of protein glycation is crucial for elucidating their relationship with diabetes mellitus and related disorders. This study explores the interaction between d -ribose and human myoglobin (HMb), as well as the protective effect of thymoquinone (TQ) on glycation. A time-dependent in-vitro glycation study was performed to investigate the mechanism of d -ribose-induced structural interference of HMb in the absence and presence of TQ. Spectroscopic and proteomic analysis indicated that the presence of TQ significantly reduced the total amount of AGEs while maintaining structural characteristics of HMb. 14 glycated sites on HMb were further identified via liquid chromatography-tandem mass spectrometry (LC-MS/MS) after incubation with d -ribose for 12 h, predominantly interacting with lysine residues. TQ was found to disrupt this interaction, reducing the glycated sites from 14 to 12 sites and the percentage of glycated peptides from 26.50 % to 12.97 %. Additionally, there was a significant decrease in the degree of glycation at the same sites. In summary, our findings suggest that TQ has the potential to act as an anti-glycation agent and provide a comprehensive understanding underlying the inhibition mechanism of glycation.
研究领域:
生物化学
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医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）