FOOD HYDROCOLLOIDS
·
2026
·
IF 13.8
Rui Hu,Da-Wen Sun,Liang Xu,Tingting Gao,You Tian
Preserving biological functionality under cryogenic conditions remains essential across biotechnology, food, and healthcare sectors, yet conventional cryoprotectants such as dimethyl sulfoxide (DMSO) and glycerol pose significant cytotoxic and environmental concerns. Herein, a sustainable cryopreservation strategy based on hydrocolloid-like micro eutecto-vesicles (MEVs), fabricated via a facile centrifugal process using glucose/fructose (GF), proline/glucose (PG), and proline/sorbitol (PS) based natural deep eutectic solvents (NADESs), and sodium alginate (SA), was developed, which was dual bioinspired by cold-tolerant organisms and frog eggs. The results showed that the introduction of NADESs can improve the colloidal stability and uniformity of the MEVs preparation process, and the diameter of the MEVs obtained at 1000 rpm centrifugal speed was about 300 μm. The toxicity test for yeast showed that the components used were less toxic than glycerol and DMSO in terms of proliferation activity and ROS accumulation. For the evaluation of post-thaw cell viability, the cryoprotection of SA/PG based MEVs can achieve a survival rate of yeast cells higher than 92%, while ensuring the integrity of cell structure and excellent proliferation activity. The subsequent mechanism exploration showed that the colloidal vesicle structure of MEVs can reduce the formation of intercellular ice crystals during freezing and inhibit the recrystallization of ice crystals. This study presents a biocompatible, scalable, environmentally responsible, and food grade hydrocolloid-based platform for the long-term stabilization of biological systems, with direct implications for sustainable probiotic production, cold-chain logistics, and bioresource preservation.
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TALANTA
·
2026
·
IF 6.7
Xiao Ma,Junjie Wang,Xin Chen,Jingcheng Li,Jiaqi Xue,Yufan Hou,Shihao Zhao,Jingxiang Zhao,Xiaomi Meng,Huajun Chen,Fang Wang,Fangya Guo,Jian Sun,Dan Zhao
Oxidase-like nanozymes use dissolved O 2 as the terminal electron acceptor and do not require exogenous H 2 O 2 , offering methodological advantages; however, conventional “one-pot carbonization” often causes concurrent carbonization and active-site formation, making the coordination environment difficult to control. Here, a sequential “pre-coordinate-polymerize-pyrolyze” site-engineering route was developed. On carbon black, OPD-Fe 3+ pre-coordination and in situ oxidative polymerization were carried out, followed by thermal treatment and purification, by which Fe–N moieties were immobilized within the shell region of the material to afford highly dispersed Fe–N x moieties predominantly featuring Fe–N 4 -like coordination (Fe–N 4 @CB d ). Multiscale characterization (XRD/XPS/XANES/EXAFS) consistently showed that iron resided predominantly in a first coordination sphere dominated by Fe–N (coordination number CN ≈ 3.7; R ≈ 2.01 Å), with no resolvable Fe–Fe scattering and only minor, non-dominant Fe-containing phases. ROS-trapping and time-dependent EPR measurements further confirmed continuous O 2 activation with the generation of O 2 • - , •OH, and 1 O 2 in the Fe–N 4 @CB d /O 2 system. DFT calculations additionally supported Fe–N 4 -like Fe–N x sites as the most plausible dominant active structures for O 2 activation. Under a unified TMB/O 2 system, steady-state enzyme kinetics exhibited outstanding performance, with V max = 351 nmol L −1 s −1 and K m = 0.56 mM, which are competitive among reports using the same substrate/readout. An H 2 O 2 -free colorimetric platform for alkaline phosphatase (ALP) was constructed on this material, achieving a low limit of detection and good linearity; acceptable accuracy and precision were verified by standard additions in diluted serum. This sequential strategy predefines the Fe–N neighborhood and topology at the precursor stage, reduces uncertainty in site formation, and avoids metal-organic framework (MOF)/hard-template/ionic-liquid multi-step procedures, thereby providing a strong basis for reproducible Fe–N 4 -like/Fe–N x oxidase-like nanozyme preparation and streamlined, robust bioanalysis.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Xi Peng,Xing Hu,Kai Liu,Deming Gong,Guowen Zhang
Inhibition of advanced glycation end products (AGEs) formed in protein glycosylation is crucial for minimizing diabetic complications. Herein, the anti-glycation potential of hesperetin-Cu (II) complex was investigated. Hesperetin-Cu (II) complex strongly inhibited three stages glycosylation products in bovine serum albumin (BSA)-fructose model, especially for the inhibition of AGEs (88.45%), which was stronger than hesperetin (51.76%) and aminoguanidine (22.89%). Meanwhile, hesperetin-Cu (II) complex decreased the levels of BSA carbonylation and oxidation products. 182.50 µg/mL of hesperetin-Cu (II) complex inhibited 66.71% β-crosslinking structures of BSA, and scavenged 59.80% superoxide anions and 79.76% hydroxyl radicals. Moreover, after incubating with methylglyoxal for 24 h, hesperetin-Cu (II) complex removed 85.70% methylglyoxal. The mechanisms of protein antiglycation by hesperetin-Cu (II) complex may be through protecting structure, trapping methylglyoxal, scavenging free radicals and interacting with BSA. This study may contribute to the development of hesperetin-Cu (II) complex as a functional food additive against protein glycation.
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Analytical Methods
·
2023
·Vol. 15
·期 12
·1569-1575
·
IF 3.1
Qianji Li,Xiwen Wu,Xiaohuan Zhang,Wenen Su,Yan Tan,Pengfei Fan,Jinquan Liu,Shengyuan Yang
Eco-friendly biomass carbon dots (CDs) with blue fluorescence emission were rapidly synthesized by a microwave method. Based on the inner filter effect (IFE) between oxytetracycline (OTC) and CDs, the fluorescence of CDs could be selectively quenched by OTC. Therefore, a simple and time-saving fluorescence sensing system for the detection of OTC was established. Under optimal experimental conditions, the concentration of OTC showed a good linear relationship with fluorescence quenching values (ΔF) in the range of 4.0–100.0 μmol L−1, a corresponding correlation coefficient (r) of 0.9975, and a detection limit of 0.12 μmol L−1. The method has the advantages of low cost, time-saving, and green synthesis that could be used for the determination of OTC. Moreover, possessing high sensitivity and specificity, this fluorescence sensing method was successfully applied for detecting OTC in milk, indicating its potential applications in food safety.
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CHEMISTRY-A EUROPEAN JOURNAL
·
2023
·Vol. 29
·期 34
·e202300454
·
IF 4.3
Henglong Xiang,Sijun Huang,Dongwei Zhu,Liqiang Yu,Rui Liu,Yi Guo,Li Xu
Graphical [Ag(NH 3 ) 2 ] + can be directly used as an electron acceptor for the oxidation of glucose by AuNPs to accelerate Tollens′ reaction, The formed Ag nanoparticles continued to act as a cascading catalyst to accelerate the reaction and indicator, which provides a possibility for the rapid colorimetric glucose detection. Au nanoparticles (NPs) have been proven to be excellent glucose oxidase (GOx) mimics, which can catalyze the electrons transform pathway from glucose to oxygen. This study confirmed AuNPs can accelerate the reaction between [Ag(NH 3 ) 2 ] + and glucose under alkaline conditions, which is also known as the Tollens′ reaction, and the possible mechanism was proposed. Here, [Ag(NH 3 ) 2 ] + instead of O 2 acted directedly as an electron acceptor during glucose oxidation catalyzed by AuNPs, accompanied by hydrogen transfer. The as-synthesized Ag nanoparticles can also catalyze this process, similar to AuNPs, via a unique cascading catalysis mechanism in the Tollens′ reaction. A simple and heatless glucose colorimetric assay can be established based on the plasmonic band of AgNPs with a liner range of 0.6–22.2 μM, and the limit of detection is 0.32 μM.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Xiaojuan Yi,Yonghua Yuan,Min Qing,Lulu Wang,Huizhen Li,Lijuan Bai
Glucose management is an important part of disease control for diabetes patients, thus the development of a rapid and real-time point of care testing (POCT) device for monitoring blood glucose is of great significance. In this work, a paper-based analytical device (PAD) is constructed by combining acetylene black (AB)-hemin complex modified filter paper as sensing platform with a smartphone as signal detector. Large specific surface area of AB decreases the self-associate and aggregate of hemin in aqueous solution, resulting in improved peroxidase-like activity of hemin. Compared with graphene oxide supported hemin, AB-hemin exhibits superior signal response on paper. Glucose oxidase (GOx) catalyzes the conversion of blood glucose to hydrogen peroxide, and then AB-hemin complex catalyzes the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue TMB oxidized products (TMB + ) in the presence of hydrogen peroxide, thus achieving the visual detection of blood glucose. In optimal conditions, PAD provides an applicable linear range from 0.2 mM to 30 mM and a low limit of detection (LOD) (0.06 mM). Notably, the detection accuracy of the developed paper-based sensor is in good agreement with that of the commercially available blood glucose meter (p > 0.05). Moreover, the proposed PAD presents high recoveries from 95.4% to 112% (RSD ≤ 3.2%), and therefore holds great potential for glucose monitoring and diabetes diagnosis.
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NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 17
·7957-7960
·
IF 3.3
Zhicheng Luo,Zhiguo Zhu,Lupeng Han,Rui Xiao,Dawang Chu
WOx/ZrO2 is effective in the hydrogenolysis of cellulose to C3 products but the active sites on the W–Zr interfaces are hardly controlled. Herein, we prepared WOx/ZrO2 catalysts with active WOx species by simply varying the reduction temperatures, which selectively achieved C3 alcohol formation. A series of characterizations showed that increased reduction temperatures (400–600 °C) led to the formation of more dispersed WOx, while higher reduction temperatures (>600 °C) contributed to producing aggregated WO3 and W2C. At 600 °C, the dispersity of WOx peaked with a W5+/∑Wn+ ratio of 19%, providing abundant Lewis acid sites and oxygen vacancies (Vo), which were the active sites for glucose isomerization to fructose and C–C cleavage of fructose. A selectivity of 48% for C3 alcohols was obtained over the optimized WOx/ZrO2(600 °C) + Ru/AC catalyst.
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Nanomaterials
·
2023
·Vol. 13
·期 6
·1073
·
IF 5.3
Shuangyin Zeng,Shaojie Han,Xiaotian Sun,Li Wang,Yanfeng Gao,Zhang Chen,Haitao Feng
Carbon materials derived from natural biomaterials have received increasing attention because of their low cost, accessibility, and renewability. In this work, porous carbon (DPC) material prepared from D-fructose was used to make a DPC/Co3O4composite microwave absorbing material. Their electromagnetic wave absorption properties were thoroughly investigated. The results show that the composition of Co3O4nanoparticles with DPC had enhanced microwave absorption (−60 dB to −63.7 dB), reduced the frequency of the maximum reflection loss (RL) (16.9 GHz to 9.2 GHz), and had high reflection loss over a wide range of coating thicknesses (2.78–4.84 mm, highest reflection loss <−30 dB). This work provided a way for further research on the development of biomass-derived carbon as a sustainable, lightweight, high-performance microwave absorber for practical applications.Keywords:biomass porous carbon;Co3O4;microwave absorbing;impedance matching;dielectric loss
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ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 33
·2301727
·
IF 19
Amjad Farid,Chen Zhonghua,Abdul Sammed Khan,Muhammad Javid,Ijaz Ahmad Khan,Aqib Ali Khan,Zeng Fan,Lujun Pan
High fabrication cost, chemical instability, and complex immobilization of enzyme molecules are critical issues of enzyme-based glucose sensors. Designing state-of-the-art, binder-free, and non-enzymatic glucose sensing probes plays an imperative role to cope with the aforementioned issues. 3D carbonaceous nanomaterials coated with transition metal vanadates (TMVs) are a favorable biomimetic platform for glucose quantification. Peculiar hierarchical structure, enhanced conductivity, synergistic interaction, multiple oxidation states, and high catalytic activity would make such composite a potential contender for non-enzymatic glucose sensing. Herein, 3D helical-shaped carbon nanocoils (CNCs) are grown on nickel foam (NF) via chemical vapor deposition method to prepare a robust CNCs/NF scaffold. Then, a hydrothermal route is followed to grow interconnected free-standing Ni 3 V 2 O 8 nanosheets (NSs) on CNCs/NF scaffold. This novel and binder-free Ni 3 V 2 O 8 NSs/CNCs/NF hierarchical composite possesses superior electrochemical active area (ECSA) and exceptional electrochemical efficacy. Amperometric analysis exhibits extremely prompt detection time (0.1 s), elevated sensitivity (5214 µA mM −1 cm −2 ), and low detection limit (0.04 µM). Developed sensor demonstrates appreciable recoveries (93.3 to 103.3%) regarding glucose concentration in human serum. The appealing analytical results show that deployment of a 3D helical-shaped hierarchical smart scaffold can be an effective strategy for developing efficient and advanced non-enzymatic glucose sensors.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Ji-hua Mao,Kai Zhang,Ying-fei He,Jun Liu,Yan-hong Shao,Zong-cai Tu
Ovalbumin (OVA) was modified by fructose (Fru) and galactose (Gal) to study the structure, IgG/IgE binding capacity and effects on human intestinal microbiota of the conjugated products. Compared with OVA-Fru, OVA-Gal has a lower IgG/IgE binding capacity. The reduction of OVA is not only associated with the glycation of R84, K92, K206, K263, K322 and R381 in the linear epitopes, but also with conformational epitope changes, manifested as secondary and tertiary structural changes caused by Gal glycation. In addition, OVA-Gal could alter the structure and abundance of gut microbiota at phylum, family, and genus levels and restore the abundance of bacteria associated with allergenicity , such as Barnesiella , Christensenellaceae_R-7_group , and Collinsela , thereby reducing allergic reactions. These results indicate that OVA-Gal glycation can reduce the IgE binding capacity of OVA and change the structure of human intestinal microbiota. Therefore, Gal glycation may be a potential method to reduce protein allergenicity.
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ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Lei Wang,Kecai Chen,Peng Zheng,Xiang Huo,Fei Liao,Liping Zhu,Meirong Hu,Yong Tao
D -Psicose, a new-generation sugar substitute, has been enzymatically synthesized through D -fructose isomerization. However, isomerization often causes low yields due to unfavorable thermodynamic equilibria, which limited its further industrial application. In this study, we present a redox-driven multi-enzyme cascade, two-step biotransformation system to produce D -psicose from D -fructose. Compared to D -fructose isomerization, this method has a maximized theoretical conversion rate of 100%. D -Psicose-3-epimerase from Clostridiales (CBDPE), ribitol 2-dehydrogenase from Providencia alcalifaciens (PRDH), and formate dehydrogenase from Starkeya (SFDH) were co-expressed in Escherichia coli in the first step to produce D -allitol from D -fructose. Afterward, NADH oxidase from Streptococcus pyogenes (SPNOX), and ribitol 2-dehydrogenase from Rubrivivax sp. (RSRDH) were co-expressed in E. coli to oxidize D -allitol into D -psicose in the second step. The two-step biotransformation system was optimized to maximize the D -fructose-to- D -psicose conversion rate (up to 90%), corresponding to a concentration of 450 mM. This study suggests that this redox-driven multi-enzyme cascade strategy through a sugar-to-alcohol-to-sugar pathway has the advantage of great application for enhanced production of D -psicose and other rare sugars.
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CELLULOSE
·
2022
·Vol. 30
·期 2
·801-813
·
IF 6.123
Wei Chunjie,Liu Gang,Xie Yujiao,Sun Zhongyuan,Liu Chang,Song Feng,Cui Hongyou
Molten salt hydrate (MSH) is a good solvent for cellulose dissolution and an effective catalyst for cellulose conversion. In this work, a series of MSH was evaluated in terms of cellulose dissolvability, regeneration performance, and catalytic activity in cellulose conversion into 5-hydroxymethylfurfural (HMF). ZnBr 2 ·3H 2 O–LiCl·3H 2 O showed good performance on cellulose dissolution and conversion. The effects of ZnBr 2 ·3H 2 O concentration, reaction temperature, and time on cellulose conversion into HMF in ZnBr 2 ·3H 2 O–LiCl·3H 2 O were optimized, and 48.7% HMF yield was reached at 60 wt.% ZnBr 2 ·3H 2 O. The species of ZnBr 2 ·3H 2 O–LiCl·3H 2 O were analyzed, and the function of species concentration was correlated with the product yield from the conversion of fructose. It was found that Br − and H + were the active species for fructose dehydration into HMF. ZnBr 3 − and H + catalyzed the conversion of fructose into furfural, which competed with the production of HMF. The reaction mechanism was proposed to understand the cooperate of species in MSH for cellulose conversion.
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