JOURNAL OF MOLECULAR LIQUIDS
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2023
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IF 6
Binbin Zhang,Jiao Ma,Kaide Ou,Xin Cong,Hao Zou,Yonggui Liao,Yajiang Yang,Hong Wang
By loading 3-nitrophenylboronic acid (3-NPBA) which can react with saccharide, the fluorescence lanthanide metallogel can emit fluorescence signals at 398, 490 and 546 nm. Thus, a three-dimensional responsive fluorescence sensor array (H 6 L/Tb 3+ /3-NPBA) was prepared to recognize saccharides. Due to the typical pH responsiveness of the fluorescence behavior of the sensor array, it is suitable for H + generation systems, such as the reaction of 3-NPBA with cis -diol of saccharides. The response of fluorescence signal intensity to 15 different saccharides was measured, and the qualitative discrimination of those saccharides was investigated by principal component analysis (PCA). The results showed that PCA elliptic clusters did not overlap, even though the concentration of the saccharides was as low as 3 mmol/L, and the discrimination rate of each saccharide reached 100 %. The “double blind” experiment indicated that the sensor system has good ability to discriminate unknown component samples. Furthermore, the discrimination rate of fructose/glucose mixtures with varied mole ratios also reached 100 %. The method of Euclidean distance confirmed that there was a good linear correlation between PCA elliptic clusters of fructose and its concentration, which could realize the quantitative analysis of fructose. The results of PCA indicated that the elliptic clusters of tea drinks with various sugars and commercially available sugar-containing drinks were loosely distributed, which further confirmed that the sensor system can effectively discriminate the saccharides under the interference of complex components.
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Journal of Functional Foods
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2022
·
IF 5.223
Hemei Li,Jinling Zhou,Sitong Liu,Xiuting Chen,Tiantian Qin,Guodong Huang,Peizhen Luo,Ying Hu,Xing Xia
Cinnamomum cassia Presl (CCP) has a beneficial impact on diabetes and dementia. In this research, the underlying mechanism of flavonoids of Cinnamomum cassia Presl (CFS) on diabetes-related cognitive impairment (DRCI) were inferred by a network pharmacology approach. The conjectures of network study were verified through the advanced glycation end products (AGEs) systems in vitro and the long-term diabetic rats. The experimental results showed CFS inhibited AGEs generated from nonenzymatic glycosylation reaction in vitro as well as in the brain of long-term diabetic rats in a dose-dependently manner. In morris water maze study, CFS restored the cognitive ability of diabetic rats. Moreover, the hippocampal morphology damage and oxidative stress were ameliorated by CFS, while the hippocampal level of AGEs, RAGE and pJNK/JNK were decreased significantly. These results indicated CFS may suppress AGEs accumulation via inhibiting glycosylation, and alleviating oxidative stress caused by AGEs, thereby prevents the neuronal injury and ameliorates DRCI.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2022
·Vol. 57
·期 6
·3385-3399
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IF 3.612
Yu Zhang,Kexin Li,Maobin Chen,Shangling Fang,Da Zhen,Jinghua Cao,Zhengqi Wu,Ke Zhang
Summary In this study, a novel chrysanthemum polysaccharide (CP) was obtained and investigated from Chrysanthemum morifolium tea in Hubei province, China. Molecular characterisation and emulsifying properties of the CP were elucidated. The effects of different factors, including storage time, metal ions, pH value and heat treatment, were studied on the stability of emulsion. Results showed that the mean particle diameter (MPD) ( d 32 ) varied from 5.54 to 0.49 μm with the polysaccharide concentration ranging from 0.5wt% to 3.0wt%. The MPD ( d 32 ) of emulsions stabilised by chrysanthemum tea polysaccharide (ECP) was smaller than that of GA at 2wt%, and the MPD ( d 32 ) of ECP remained stable with no stratification occurred at 25 °C for 10 days. ECP also showed good stability under acidic conditions (3.0–7.0). With increase of Na + and Ca 2+ concentration, MPD( d 32 ) and zeta potential values raised significantly, from 2.24 to 7.24 μm and from −40.52 to −35.35 mV respectively. After high-temperature heat treatment, MPD ( d 32 ) and zeta potential showed no significant change, indicating ECP presented good storage stability. In conclusion, CP helped stabilised emulsions that could be used as a natural emulsifier for future application.
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Marine Drugs
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2022
·Vol. 20
·期 3
·177
·
IF 6.085
Ying Wang,Xiao-Ming Li,Sui-Qun Yang,Fan-Zhong Zhang,Bin-Gui Wang,Hong-Lei Li,Ling-Hong Meng
An unusual sesquiterpene glycoside trichoacorside A (1) and two novel sorbicillinoid glycosides sorbicillisides A (2) and B (3), together with a known compound sorbicillin (4), were isolated and identified from the culture extract of an endophytic fungusTrichoderma longibrachiatumEN-586, obtained from the marine red algaLaurencia obtusa. Trichoacorside A (1) is the first representative of a glucosamine-coupled acorane-type sesquiterpenoid. Their structures were elucidated based on detailed interpretation of NMR and mass spectroscopic data. The absolute configurations were determined by X-ray crystallographic analysis, chemical derivatization, and DP4+ probability analysis. The antimicrobial activities of compounds1–4against several human, aquatic, and plant pathogens were evaluated.Keywords:Trichoderma longibrachiatum;sesquiterpene glycoside;secondary metabolites;antimicrobial activity
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JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Lei Li,Yun Fang,Yongmei Xia,Chunling Bo,Ye Fan
Acted as the second component of fatty acid vesicles (FAV), almost all reported chemicals except for bio-incompatible cationic amphiphiles caused a one-side pH window extension of FAV whilst the other-side shrinkage accordingly. We recently found that dodecanol could partially compensate the alkaline-side pH window shrinkage of FAV when the acidic-side was extended by sodium dodecylsulfate (SDS). Therefore, we guess that if one single small molecule played two − in − one function as both SDS and dodecanol done on the different-side pH window, it might cause a two-side pH window extension of FAV entering into near-neutral pH. Taking conjugated linoleic acid (CLA) as the model fatty acid, ribose, glucose, glucosamine and acetyl glucosamine as the models of the second component, the influence on the pH window extension for the FAV of CLA by each of the monosaccharides and the involved noncovalent bonding interactions are investigated by laser Tyndall effect, pH titrimetry, transmission electron microscopy, dynamic light scattering, ultraviolet absorbency, isothermal titration calorimetry measurement and binding energy calculation. The two-side pH window extension for the FAV of CLA is realized by aid of the two − in − one function of monosaccharides that create H-bonding interaction with the FAV surface and thus enhancing hydrophobic self-assembly of CLA. It is crucial for self-assembling FAV as primitive cell membranes and applying it as modern nutrient carriers or drug delivery systems in the physiological pH range. We propose a practical microenvironmental polarity criterion based on the experimental results to predict how the second component to extend the pH window of FAV: If the second component made the FAV surface more hydrophobic on the alkaline-side pH window while more hydrophilic on the acidic-side via molecular interactions, it could drive the corresponding-side pH window extension, otherwise the corresponding-side pH window shrinkage.
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Food Chemistry-X
·
2022
·
IF 6.443
Hualing Xie,Jingyu Fang,Mohamed A. Farag,Zhenhao Li,Peilong Sun,Ping Shao
In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining. The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs. Cytokines levels as marker of inflammation were determined using ELISA in serum and colon. Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF- α , TGF- β 1, IL-6, IL-1 β ) and promoted the expression of anti-inflammatory cytokines (IL-10). Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing. DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides , Lactobacillus and Lachnospiraceae. Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
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Nanomaterials
·
2022
·Vol. 12
·期 9
·1389
·
IF 5.719
Jiahang Yu,Jun Qi,Zhen Li,Huixin Tian,Xinglian Xu
Monitoring food quality throughout the food supply chain is critical to ensuring global food safety and minimizing food losses. Here we find that simply by mixing an aqueous solution of sugar-stabilized Ag+and amines in an open vessel leads to the generation of Ag NPs and an intelligent evaluation system based on a colorimetric Ag+probe is developed for real-time visual monitoring of food freshness. The self-assembly reaction between methylamine (MA) generated during meat storage and the colorimetric Ag+probe produces different color changes that indicate changes in the quality of the meat. The colorimetric Ag+probe was integrated into food packaging systems for real-time monitoring of chilled broiler meat freshness. The proposed evaluation system provides a versatile approach for detecting biogenic amines and monitoring chilled broiler meat freshness and it has the advantages of high selectivity, real-time and on-site measurements, sensitivity, economy, and safety and holds great public health significance.Keywords:colorimetric Ag+probe;self-assembly;Ag NPs;amines;food detection
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Frontiers in Nutrition
·
2022
·
IF 6.59
Hong Tao,Zhao Jiaying,Yin Junyi,Nie Shaoping,Xie Mingyong
Tea is a popular beverage with a long history of safe and healthy use. Tea polysaccharide is a bioactive component extracted from tea, which has attracted more and more attention in recent decades. In this article, an acidic polysaccharide Gougunao tea polysaccharide (GPS) was isolated from Gougunao green tea by hot water extraction and ethanol precipitation. After purification by a diethylaminoethyl (DEAE) Sepharose Fast Flow column and a Sephacryl S-400 column, several homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) fractions were obtained. Fraction GPS2b with the highest yield was selected for structural characterization by methylation and nuclear magnetic resonance (NMR) analysis. GPS2b was found to be an HG-type pectic polysaccharide (degree of methyl esterification [DE], 51.6%) with low molecular weight (Mw, 36.8 kDa). It was mainly composed of →4)-α-GalpA- (1→ and →4)-α-GalpA-6-OMe-(1→. In addition, a minor highly branched RG-I domain was identified in this fraction. The investigation of structural features of tea polysaccharides can provide insights to understand their structure-bioactivity relationship.
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FOOD CHEMISTRY
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2022
·
IF 9.231
Lu-Shuang Li,Yun-Ling Gao,Jia-Li Sun,Long Chen,Jing Li
A novel preparation scheme of thioglycerol-modified silica through thiol-epoxy click reaction was proposed aiming at introducing additional quantities of hydroxyl groups into the structure. When applied as the stationary phase of hydrophilic interaction liquid chromatography (HILIC) for separation of oligosaccharide compounds, the material revealed higher polar separation capability than which synthesized through traditional thiol-ene click reaction. Hydrogen-bond interactions were speculated to be the predominant retention mechanism, while partitioning also participated in the retention of disaccharides and trisaccharides. The column also showed good stability and inter-batch reproducibility. Finally, the column was employed for determination of oligosaccharide compounds in commercial beverages, and good linearities, high accuracy, favorable precision, satisfactory reproducibility and resistance to matrix interference were achieved. In the detection of real samples, the determined content was consistent with the labeled content. This work provided an efficient and practicable method for quantity monitoring of commercial diet drinks in routine laboratory.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-14
·
IF 17.694
Arabi Maryam,Ostovan Abbas,Wang Yunqing,Mei Rongchao,Fu Longwen,Li Jinhua,Wang Xiaoyan,Chen Lingxin
Chiral discrimination is critical in environmental and life sciences. However, an ideal chiral discrimination strategy has not yet been developed because of the inevitable nonspecific binding entity of wrong enantiomers or insufficient intrinsic optical activities of chiral molecules. Here, we propose an “inspector” recognition mechanism (IRM), which is implemented on a chiral imprinted polydopamine (PDA) layer coated on surface-enhanced Raman scattering (SERS) tag layer. The IRM works based on the permeability change of the imprinted PDA after the chiral recognition and scrutiny of the permeability by an inspector molecule. Good enantiomer can specifically recognize and fully fill the chiral imprinted cavities, whereas the wrong cannot. Then a linear shape aminothiol molecule, as an inspector of the recognition status is introduced, which can only percolate through the vacant and nonspecifically occupied cavities, inducing the SERS signal to decrease. Accordingly, chirality information exclusively stems from good enantiomer specific binding, while nonspecific recognition of wrong enantiomer is curbed. The IRM benefits from sensitivity and versatility, enabling absolute discrimination of a wide variety of chiral molecules regardless of size, functional groups, polarities, optical activities, Raman scattering, and the number of chiral centers.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Qingxia Yuan,Hong Li,Qian Wang,Shujing Sun,Ziyu Fang,Hao Tang,Xiaohuo Shi,Jing Wen,Lianghua Huang,Meng Bai,Shengping Zhong,Yonghong Liu,Chenghai Gao,Longyan Zhao
Stichopus monotuberculatus is a tropical sea cucumber species and used as a folk medicine and tonic food . In this study, a fucosylated glycosaminoglycan (SmFG), the depolymerized SmFG (dSmFG) and its oligosaccharide fractions were prepared. The SmFG and its depolymerized products were comprised of a chondroitin-sulfate-E backbone, and various sulfated fucose side chains, including an unusual disaccharide side chain connected to the C-3 position of D-glucuronic acid (GlcA) or GlcA-ol. A peeling reaction occurred during the deaminative depolymerization process. The dSmFG and its fractions showed strong anticoagulant activity by selectively inhibiting intrinsic tenase complex, and had no anti-factor IIa, Xa and VIIa activity. The anticoagulant activity reduced with the decrease of molecular weight , and the unusual branch and novel reducing end may enhance the anticoagulant activity. These findings can provide significant information for development and utilization of depolymerized products from SmFG in food and pharmaceutical industries .
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PROCESS BIOCHEMISTRY
·
2022
·
IF 4.885
Zhengyu Hu,Ruiying Yu,Jinfeng Sun,Yuanqi Duan,Hongli Zhou,Wei Zhou,Gao Li
In this study, polysaccharides from the leaves of Rhododendron dauricum L. (RDPs) were decolorized using the static macroporous resin adsorption method. The RDPs were characterized and their antioxidant activities were investigated. An analytical hierarchy process (AHP) and a response surface method (RSM) were used to determine the optimum decolorization conditions as: a polysaccharide concentration of 8 mg/mL, decolorization time of 70 min, liquid–solid ratio of 6 mL/g, and decolorization temperature of 52 °C, with D101 as the best resin. Furthermore, two novel polysaccharides (RDP-1 and RDP-2) were isolated from R. dauricum leaves and characterized, which demonstrated that RDP-1 and RDP-2 were both composed of mannose , glucose, galactose , and arabinose in different molar ratios. These RDPs also exhibited a triple helix conformation, and primarily contained →6)-Glc p -(1→, →5)-Ara f -(1→, →2,4)-Man p -(1→, →6)-Gal p -(1→ and Glc p -(1→ residues with different connection sequences. Biological activity tests showed that the two polysaccharides possessed scavenging capacity against 1,1-Diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and 2,2-Azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radicals. The results indicated that the antioxidant activities of the RDPs obtained by the static decolorization method improved, which lays a theoretical foundation for developing techniques for utilising R. dauricum .
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