RSC Advances
·
2021
·Vol. 11
·期 43
·26594-26606
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IF 3.361
Yang Yu,Shifei Wu,Jianqing Zhang,Jiayuan Li,Changliang Yao,Wenyong Wu,Yingying Wang,Hongjian Ji,Wenlong Wei,Min Gao,Yun Li,Shuai Yao,Yong Huang,Qirui Bi,Hua Qu,De-an Guo
Thirty-seven diterpenoid alkaloids (DAs) with diverse structures were isolated and identified from the lateral roots of Aconitum carmichaelii Debx., comprising eight C20-DAs and twenty-nine C19-DAs. Besides the 31 known DAs identified by comparing the 1H NMR and 13C NMR data with those reported in the literature, the structures of four new compounds (1, 14, 17, and 25), and two other compounds (26 and 37) which were reported to be synthesized previously, were also elucidated based on the comprehensive analysis of their HR-ESI-MS, 1D and 2D NMR spectra, including 1H–1H COSY, HSQC and HMBC and NOESY/ROESY. Among them, compound 1 represents the first example of a C20-DA glucoside. Besides, the anti-tumor activities of all the isolated compounds against human non-small-cell lung cancer A549 and H460 cells were systematically evaluated by MTT methods. The results revealed that all of the C19-DAs possessed moderate activities against both of the two cell lines with IC50 values ranging from 7.97 to 28.42 μM, and their structure–activity relationships indicated the active sites of C-8, C-10, and C-14 positions and the nitrogen atom in the C19-DA skeleton. In addition, all of the isolated DAs, with chemical structures confirmed, were further applied for network pharmacology analysis, in order to give an insight into the possible mechanisms of their anti-tumor activities. As a result, 173 potential targets and three most important pathways related to non-small-cell lung carcinoma were finally unearthed.
研究领域:
药理学
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化学
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肿瘤学
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植物科学
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DOI
PubMed
高影响因子
Scientific Reports
·
2021
·Vol. 11
·期 1
·1-13
·
IF 4.38
Jiao Jianhang,Peng Chuangang,Li Chen,Qi Zhiping,Zhan Jing,Pan Su
Electrospun fibrous scaffolds combined with bioactive factors can display impressive performance as an ideal wound dressing, since they can mimic the composition and physicochemical properties of the extracellular matrix (ECM). The aim of this study was to fabricate a new composite biomaterial (IGF1-DA and Os-DA-modified PLGA electrospun fibrous scaffold) for wound healing, using a rat model for experimental evaluation. A small pentapeptide tag composed of DA–Lys–DA–Lys–DA residues was introduced into insulin-like growth factor 1 (IGF1) and the antimicrobial peptide Os to prepare IGF1 and Os modified with 3,4-dihydroxyphenylalanine (DA) (IGF1-DA and Os-DA). The designed chimeric growth factor and antimicrobial peptide could successfully anchor to PLGA electrospun fibrous scaffolds, and the growth factor and antimicrobial peptide could be controllably released from the electrospun fibrous scaffolds. The results showed that the IGF1-DA and Os-DA-modified PLGA electrospun fibrous scaffolds (PLGA/Os-DA/IGF1-DA) exhibited high hydrophilicity and antimicrobial activity; moreover, the porous network of the scaffolds was similar to that of the natural ECM, which can provide a favourable environment for BALB/C 3T3 cells growth. The in vivo application of PLGA/Os-DA/IGF1-DA electrospun fibrous scaffolds in rat skin wounds resulted in improved wound recovery and tissue regeneration rate. The experimental results indicated that the IGF1-DA and Os-DA could effectively bind to PLGA electrospun fibrous scaffolds, promote wound healing and prevent infection in rats, thereby suggesting that PLGA/Os-DA/IGF1-DA electrospun fibrous scaffolds have a wide application value in the field of skin wound repair.
研究领域:
生物医学工程
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组织工程
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材料科学
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伤口愈合研究
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产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2012
·
IF 2.739
Qingye Liu,Xiaojuan Xu,Lina Zhang,Junping Yu
The interaction between polydeoxyadenylic acid (poly(dA)) and single chains of Lentinan (s-LNT) was investigated by circular dichroism spectra (CD), UV–Vis spectra, nano-differential scanning calorimetry (nano-DSC), dynamic light scattering (DLS), and atomic force microscopy (AFM). All the experimental results indicated that poly(dA) really interacted with s-LNT having molecular weight of 5.3 × 10 5 to form a novel composite with stiff conformation through hydrogen bonding , whereas the triple helical Lentinan (t-LNT) could not, implying that it was the single chain but not the triple helical chain interacted with poly(dA). Meanwhile, the interaction strongly depended on the concentration of s-LNT and pH. When the poly(dA) concentration was fixed at 5.3 μg/ml, the interaction between poly(dA) and s-LNT increased with an increase in s-LNT concentration, then reached the maximum at ∼60 μg/ml, finally decreased with further increase of s-LNT concentration. This was due to self-renaturation of s-LNT into triple helix or incomplete species confirmed by nano-DSC. The poly(dA)/s-LNT complex could exist in solutions with pH 5.5–11.5, and pH 7–10 was the optimal condition for the complex, showing higher stability against pH than the positive control of poly(dA)/s-SPG. The dynamic behaviors of the complex demonstrated that the interaction between s-LNT and poly(dA) occurred rapidly, and reached stable within 3–7 h. Moreover, the thermal stability of poly(dA) was enhanced by complexation with s-LNT. The topograph of the poly(dA)/s-LNT complex was shown as rod-like stiff architecture. This work enlarges the application of LNT in the biomedical field.
研究领域:
生物化学
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分子生物学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Siyuan Liu,Jingxing Liu,Zefen Wang,Zhiqi Wu,Yiliang Wei,Pengru Liu,Xiongdiao Lan,Yexin Liao,Ping Lan
Glucose oxidase (GOx) has a great application potential in the determination of glucose concentration. However, its sensitivity to the environment and poor recyclability limited its broader application. Herein, with the assistance of DA-PEG-DA, a novel immobilized GOx based on amorphous Zn-MOFs (DA-PEG-DA/ [email protected] /PDA) was developed to impart excellent properties to the enzyme. SEM, TEM, XRD, and BET analyses confirmed that GOx was embedded in amorphous ZIF-7 with ∼5 wt% loading. Compared with free GOx, DA-PEG-DA/ [email protected] /PDA exhibited enhanced stability, excellent reusability, and promising potential for glucose detection. After 10 repetitions, the catalytic activity of DA-PEG-DA/ [email protected] /PDA can maintain 95.53 % ± 3.16 %. In understanding the in situ embedding of GOx in ZIF-7, the interaction of zinc ion and benzimidazole with GOx was studied by using molecular docking and multi-spectral methods. Results showed that zinc ions and benzimidazole had multiple binding sites on the enzyme, which induced the accelerated synthesis of ZIF-7 around the enzyme. During binding, the structure of the enzyme changes, but such changes hardly affect the activity of the enzyme. This study provides not only a preparation strategy of immobilized enzyme with high activity, high stability, and low enzyme leakage rate for glucose detection, but also a more comprehensive understanding of the formation of immobilized enzymes using the in situ embedding strategy.
研究领域:
生物化学
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材料科学
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催化
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酶工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 23
·14903
·
IF 6.208
Henan Shi,Zhuqing Wang,Fujie Xu,Jialin Li,Jing Li,Manyuan Wang
In 1972, Nobel laureate Youyou Tu’s research team conducted clinical trials on the dried material ofArtemisia annuaL. from Beijing extracted by ether and then treated with alkali (called “ether neutral dry”), which showed that artemisinin was not the only antimalarial component contained. The biosynthesis of sesquiterpenoids inA. annuahas increased exponentially after unremitting cultivation efforts, and the plant resources are now quite different from those in the 1970s. In consideration of emerging artemisinin resistance, it is of great theoretical and practical value to further study the antimalarial activity ofA. annuaand explore its causes. The purpose of this study is to clarify scientific questions, such as “What ingredients are synergistic with artemisinin inA. annua?”, and “Are there non-artemisinin antimalarial ingredients inA. annua?”. In this paper, Beijing wildA. annuawas used as a control and two representative cultivation species ofA. annuawere selected to evaluate the antimalarial activity of the herbal medicine. The antimalarial activity of different extracts on mice was studied using the Peters’ four-day suppressive test. UPLC-Q-TOF-MS was used to obtain mass spectrum data for all samples, and a UNIFI platform was used for identification. A multivariate statistical method was used to screen the different compounds with positive correlations. The antimalarial activity of different components from the ether extract and alkali treatments was determined and antimalarial components other than artemisinin were obtained. A total of 24 flavonoids, 68 sesquiterpenoids and 21 other compounds were identified. Compounds associated with differential antimalarial activity were identified. The material basis for the antimalarial activity ofA. annuawas clarified. The antimalarial components ofA. annuainclude two categories: first, artemisinin and non-artemisinin antimalarial active components, of which the non-artemisinin antimalarial active components may include 5α-hydroperoxy-eudesma-4(15),11-diene; second, several antimalarial synergistic ingredients inA. annua, including arteanniun B, arteanniun B analogues and polymethoxy flavonoids.Keywords:Artemisia annuaL.;synergistic effect;antimalarial;UPLC-Q-TOF-MS;OPLS-DA
研究领域:
药理学
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代谢组学
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药物化学
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植物科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2018
·
IF 4.946
Chong Shen,Bin Peng,Ying Wang,Qin Meng
Milk is often polluted by heavy metal ions due to the growing environment pollution, but few methods have been developed to remove the heavy metal ions. Here a non-toxic sorbent, namely di-acrylated Pluronic P123 (P123-DA) hydrogel, was fabricated for removal of Hg 2+ and Pb 2+ from milk without impairing their nutritive contents. This hydrogel possessed high mechanical stress and maximum adsorption capacity of 35.2 and 53.9 mg/g for Pb 2+ and Hg 2+ . The removal ratio of Pb 2+ and Hg 2+ by P123-DA hydrogel respectively reached 85.3% and 81.9% for the polluted whole milk while was individually 86.3% and 83.8% for the skim milk. Interestingly, the treatment by P123-DA hydrogel didn’t significantly reduce the main nutritive contents in milk. Such hydrogel will be a recyclable, safe and effective tool for reuse of milk that polluted by heavy metal ions.
研究领域:
食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 7
·1455
·
IF 5.2
Junting Yu,Zhilei Zhou,Xibiao Xu,Huan Ren,Min Gong,Zhongwei Ji,Shuangping Liu,Zhiming Hu,Jian Mao
Huangjiuis one of the oldest alcoholic beverages in the world. It is usually made by fermenting grains, and Qu is used as a saccharifying and fermenting agent. In this study, we identified differential carbonyl compounds inHuangjiuwith varying sugar contents from different regions. First, we developed and validated a detection method for volatile carbonyl compounds inHuangjiu, and for optimal extraction, 5 mL ofHuangjiuand 1.3 g/L of O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) were incubated at 45 °C for 5 min before extracting the volatile carbonyl compounds at 45 °C for 35 min. Second, the targeted quantitative analysis of 50 carbonyl compounds inHuangjiushowed high levels of Strecker aldehydes and furans. Finally, orthogonal projections to latent structures discriminant analysis (OPLS-DA) was used to differentiate betweenHuangjiuwith different sugar contents, raw materials, and region of origin. A total of 19 differential carbonyl compounds (VIP > 1,p< 0.05) were found inHuangjiuwith different sugar contents (semidry and semisweetHuangjiu), and 20 differential carbonyl compounds (VIP > 1,p< 0.05) were found in different raw materials forHuangjiuproduction (rice and nonriceHuangjiu). A total of twenty-two and eight differential carbonyl compounds, with VIP > 1 andp< 0.05, were identified in semidry and semisweetHuangjiufrom different regions (Zhejiang, Jiangsu, Shanghai, and Fujian), respectively.Keywords:Huangjiu;carbonyl compounds;PFBHA;targeted metabolomics;OPLS-DA
研究领域:
食品科学
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分析化学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Peihong Deng,Nana Tang,Shuting Shi,Chuanqin Zhou,Aiting Chen,Quanguo He
In this study, a preparation of hollow nanoscale tubular polysulfide Co 9 S 8 is reported, along with its use in building an electrochemical sensor that can detect dopamine (DA). Utilizing electrochemically reduced graphene oxide (ErGO) as a substrate, the Co 9 S 8 -ErGO/GCE sensor was made by attaching Co 9 S 8 nanotubes to a glassy carbon electrode (GCE) surface. The use of ErGO in the sensor fabrication process not only improves the conductivity but also effectively prevents the aggregation of Co 9 S 8 . A large number of hollow Co 9 S 8 nanotubes are anchored on ErGO, providing more catalytic active sites for electrode reactions. The constructed sensor shows remarkable DA detection ability, with a notable rise in peak current, thanks to the synergistic action of Co 9 S 8 and ErGO. This sensor has a lower detection limit of 4.0 nM and a wide linear range of 0.01–0.1 µM, 0.1–2.0 µM, and 2.0–10 µM. It is also possible to quantify DA in actual samples using Co 9 S 8 -ErGO/GCE, meeting clinical requirements for reproducibility, selectivity and accuracy.
研究领域:
材料科学
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生物医学工程
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分析化学
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产品关联
DOI
高影响因子
Surfaces and Interfaces
·
2024
·
IF 5.7
Jian Gao,Hao Li,Weixiang Sun,Xiaowen Huang,Kai Zhang
Despite significant advancements in the development of superhydrophobic coatings on metal substrates, producing durable superhydrophobic coatings on a large scale remains a formidable challenge through a simple and low-cost method. In this study, a robust and environmentally friendly superhydrophobic coating (DTSC) was developed and prepared on the surface of X80 Pipeline Steel through a spray method using a multiple bonding between phytic acid and silane coupling agent and the van der Waals force of PDMS. The morphology, structure and composition of the DTSC were analyzed by field emission scanning electron microscope, three-dimensional profilometer, and Fourier transform infrared spectroscopy. The water contact angle of the DTSC is 158°, and the roll-off angle is 2° Regardless of whether the coating is subjected to 50 cycles of abrasive paper or 14 h of water drops, it still retains excellent superhydrophobicity. It is worth mentioning that the oil-water separation capacity of the superhydrophobic steel mesh is as high as 97 %. In a range of polluted environments, superhydrophobic coatings still exhibit exceptional static and dynamic antifouling performance, as well as excellent self-cleaning performance. Furthermore, the coating has a high level of acid and alkali resistance and effectively resists ultraviolet radiation and high temperatures. This work provides an environmentally and economically viable method for the industrial application of superhydrophobic coatings.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2026
·
IF 5.9
Yuhan Zhao,Xinyu Han,Yunjie Li,Zhongliang Wang,Fang Chai
Dopamine (DA) as an important biomarker for assessing mood and neurological disorders, hydrogen peroxide (H 2 O 2 ) serves as a marker of oxidative stress and antimicrobial activity. The levels of DA and H 2 O 2 are associated with the development of serious neurological diseases. Therefore, precise prompt and portable detection for DA and H 2 O 2 is of great importance for clinical diagnosis and treatment, but maintaining a major challenge. In this work, bimetallic nanozyme CuMo nanosheets (CMNS) with peroxidase(POD)-like activity were fabricated by using tryptophan as ligand, and achieved sequential detection of H 2 O 2 and DA via a sequential process with limits of detection at 4.7 μM and 0.11 μM, respectively. A portable smartphone-based swab sensing system was also developed for rapid, on-site, and visual monitoring of DA with limit of detection (LOD) of 0.587 μM. This platform performed portable sensing DA with high accuracy in serum and milk samples, achieving recoveries between 93.02% and 108.2% with a relative standard deviation below 3.02%, indicated the excellent environmental tolerance. A molecular logic gate was also designed to understand and effectively apply the sequential sensing process. Overall, this work established a highly sensitive, selective, and portable colorimetric biosensing platform based on bimetallic nanozymes, demonstrated significant potential for practical application in diagnosis and biomarker monitoring. Such bimetallic nanozyme have great potential for future visible sensing in environmental and medical applications.
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DOI
高影响因子
IEEE TRANSACTIONS ON DIELECTRICS AND ELECTRICAL INSULATION
·
2026
·
IF 3.3
Bo Zhu,Bo Yang,Yu Feng,Wenliang Xu,Zhiqing Liang
Cross-linked polyethylene (XLPE) is widely used in high-voltage cable insulation for its good insulation and mechanical properties. However, long-term use leads to damage, breakdown, and environmental pollution due to non-recyclability and high thermal degradation. This study designs an S-S-DA cross-linker with reversible Diels-Alder (DA) and disulfide bonds using isocyanate reactions. Graft cross-linking forms XLPE-S-S-DA, and doping silane-modified SiO2 creates XLPE-S-S-DA/SiO2 composite for self-repair. Tests show elongation at break increases by 17.89%, tensile yield stress by 17.26%, and breakdown field strength by 34.4% compared to XLPE. It self-repairs mechanical/electrical damages (three mechanical repairs restore strain to 91.26%, yield stress to 82.65%, strength to 92.37%; three breakdowns restore field strength to 76.04%). Three recycling retains 68.89% strain, 71.59% strength (yield stress increases 6.46%), and 79.57% breakdown strength. Lower thermal degradation helps recycling. Dynamic cross-linking and doping create self-repair, recyclable XLPE composites, improving service life and eco-friendliness for high-voltage cable insulation, providing a new design strategy.
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Miao Yu,Yang Lu,Zhenjiang Tan
In this work, a novel and facile ratiometric fluorescence probe was prepared for the visual detection of dopamine (DA). In this detection system, red-emission CdTe@SiO 2 (r-QDs@SiO 2 ) was used as steady core of the probe and inverse microemulsion method was applied to synthesize uniform r-QDs@SiO 2 , this step could protect CdTe from contacting with human skin directly. Polydopamine (PDA) acted as response signal to detect DA, a very handy method which just combined polyethyleneimine (PEI) with DA together to synthesize PDA, this way for synthesis of PDA was much time-saving and non-toxic than any other methods. Differently from traditional analysis processes, the products of this experiment were also the analysis substances in final. Under optimum measurement conditions, the dual-emission ratiometric fluorescence probe was used for detections of DA in a concentration ranged from 10 μM to 80 μM with a detection limit of 0.12 μM, with addition of DA the color of the probe changed from red to green watched by naked eyes. In addition, the developed probe was also used for detections of DA in human serum samples successfully. This study provides a simple, time-saving and non-toxic approach for detections of DA without the requirement of complex equipment.
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）