MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·49
·
IF 5.3
Wang Chuang,Peng Yue,Miao Yuanyuan,Sun Xinyuan,Cheng Wenting,Li Jinlong
DNA methyltransferases (MTases) play a critical role in epigenetic regulation and are closely associated with the occurrence and development of various diseases. To address the limitations of current detection methods, which often suffer from complicated operation and limited sensitivity we have developed a label-free fluorescent biosensing platform based on DNA-templated silver nanoclusters (DNA-AgNCs) for the detection of DNA adenine methyltransferase (Dam MTase) activity and the screening of its inhibitors. In this system, DNA-AgNCs signal probes are synthesized via a simple reduction reaction. Dumbbell-shaped DNA substrates are designed to be specifically methylated by Dam MTase in the presence of S-adenosylmethionine (SAM). The methylated substrates are subsequently recognized and cleaved by DpnI, yielding DNA fragments with 3′-OH termini. These termini are extended by terminal deoxynucleotidyl transferase (TdT) in the presence of dGTP, generating G-rich sequences, which hybridize with the DNA-AgNCs and significantly enhance the fluorescence intensity. This cascade reaction enables the sensitive detection of Dam MTase activity. Compared with the other approaches, the proposed method exhibits a wider linear range (0.5–10 U/mL) and a lower detection limit (0.165 U/mL). Furthermore, inhibition studies demonstrate that 5-fluorouracil and gentamicin effectively suppress Dam MTase activity. The method also shows excellent performance in complex biological samples. Overall, this fluorescence-based strategy offers high sensitivity and excellent specificity, demonstrating broad application prospects in epigenetic research and drug discovery. Graphical
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ELECTROCHIMICA ACTA
·
2013
·
IF 3.777
Zhenning Xu,Mo Wang,Huanshun Yin,Shiyun Ai,Liang Wang,Junling Pang
A sensitive electrochemical method for detection of DNA methylation and assay of DNA methyltransferase (Dam) was investigated based on methylation-sensitive restriction endonuclease, DNA-Au bio bar code and enzymatic signal amplification. After the DNA probe was hybridized with complementary DNA sequence, and the obtained double-strand DNA (dsDNA) was further methylated by Dam MTase, it could be successively digested by Dpn I at the methylated sites. As a result, the bio bar code and streptavidin-horseradish peroxidase (S-HRP) could not be conjugated on the electrode surface through the specific interaction between biotin and S-HRP, where biotin was labeled at the DNA-Au bio bar code. Thus, only a low electrochemical reduction signal was obtained. However, the hybridized probe could hybrid with DNA-Au bio bar code, and then S-HRP could be conjugated on the electrode surface in the absence of Dam MTase and Dpn I, so a larger reduction current of enzymatic product was obtained. The change of the reduction current of enzymatic product was used to detect DNA methylation and Dam MTase activity. The detection limit of Dam MTase was 0.02 unit/mL (S/N = 3). Moreover, the inhibitions of mitomycin C, paclitaxel, chlorogenic acid and epicatechin were also investigated. Therefore, this method provided a sensitive platform for monitoring DNA methylation and DNA MTase activity.
研究领域:
生物化学
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分子生物学
·
医学检测
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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2024
·Vol. 121
·期 8
·e2317796121
·
IF 9.4
Xuelong Liao,Shan Chen,Jialei Chen,Youzeng Li,Wei Wang,Tiantian Lu,Zhuo Chen,Lixin Cao,Yaxin Wang,Rong Huang,Xiaoting Sun,Runyu Lv,Huan Wang
Tremendous attention has been paid to the water-associated side reactions and zinc (Zn) dendrite growth on the electrode–electrolyte interface. However, the Zn pulverization that can cause continuous depletion of active Zn metal and exacerbate hydrogen evolution is severely neglected. Here, we disclose that the excessive Zn feeding that causes incomplete crystallization is responsible for Zn pulverization formation through analyzing the thermodynamic and kinetics process of Zn deposition. On the basis, we introduce 1-ethyl-3-methylimidazolium cations (EMIm+) into the electrolyte to form a Galton-board-like three-dimensional inert-cation (3DIC) region. Modeling test shows that the 3DIC EMIm+ can induce the Zn2+ flux to follow in a Gauss distribution, thus acting as elastic sites to buffer the perpendicular diffusion of Zn2+ and direct the lateral diffusion, thus effectively avoiding the local Zn2+ accumulation and irreversible crystal formation. Consequently, anti-pulverized Zn metal deposition behavior is achieved with an average Coulombic efficiency of 99.6% at 5 mA cm−2 over 2,000 cycles and superb stability in symmetric cell over 1,200 h at −30 °C. Furthermore, the Zn||KVOH pouch cell can stably cycle over 1,200 cycles at 2 A g−1 and maintain a capacity of up to 12 mAh.
研究领域:
材料科学
·
电化学
·
能源存储
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Advanced Healthcare Materials
·
2022
·Vol. 12
·期 1
·2201661
·
IF 11.092
Zonghan Xu,Liang Wu,Yu Tang,Kun Xi,Jincheng Tang,Yichang Xu,Jingzhi Xu,Jian Lu,Kaijin Guo,Yong Gu,Liang Chen
The bone immune microenvironment (BIM) regulates bone regeneration and affects the prognosis of fractures. However, there is currently no effective strategy that can precisely modulate macrophage polarization to improve BIM for bone regeneration. Herein, a hybridized biphasic bionic periosteum, inspired by the BIM and functional structure of the natural periosteum, is presented. The gel phase is composed of genipin-crosslinked carboxymethyl chitosan and collagen self-assembled hybrid hydrogels, which act as the “dam” to intercept IL-4 released during the initial burst from the bionic periosteum fiber phase, thus maintaining the moderate inflammatory response of M1 macrophages for mesenchymal stem cell recruitment and vascular sprouting at the acute fracture. With the degradation of the gel phase, released IL-4 cooperates with collagen to promote the polarization towards M2 macrophages, which reconfigure the local microenvironment by secreting PDGF-BB and BMP-2 to improve vascular maturation and osteogenesis twofold. In rat cranial defect models, the controlled regulation of the BIM is validated with the temporal transition of the inflammatory/anti-inflammatory process to achieve faster and better bone defect repair. This strategy provides a drug delivery system that constructs a coordinated BIM, so as to break through the predicament of the contradiction between immune response and bone tissue regeneration.
研究领域:
组织工程
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再生医学
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免疫学
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生物医学工程
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骨生物学
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高影响因子
ELECTROPHORESIS
·
2018
·Vol. 40
·期 3
·425-430
·
IF 2.569
Yating Zhang,Yan Zhang,Luqi Zhu,Pingang He,Qingjiang Wang
DNA methylation is a significant epigenetic modification and the methods for the detection of DNA methyltransferase (MTase) activity are important due to aberrant methylation closely related to the occurrence of cancer. In this study, a simple and rapid microchip electrophoresis (ME) coupled with LED-induced fluorescence (LEDIF) method was presented for the detection of Dam MTase activity. This strategy was based on methylation-sensitive endonuclease DpnⅡ which could recognize the same specific site 5′-GATC-3′ with Dam MTase in double-stranded DNA (dsDNA). The adenines in the specific site could be methylated by Dam MTase, then the special site could not be digested by DpnⅡ. Both methylated dsDNA and unmethylated dsDNA could be analyzed by ME-LEDIF after stained by SYBR gold. The results showed the fluorescence intensities of methylated dsDNA were directly proportional to Dam MTase activities in the range of 0.5–20 U/mL with a detection limit of 0.12 U/mL. Furthermore, the method could successfully be applied to evaluation experiments of Dam MTase inhibitors. The results confirmed the ME-LEDIF method is a promising approach for inhibitors screening of DNA MTase and development of anticancer drugs
研究领域:
分子生物学
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分析化学
·
医学
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Guozhen Liu,Renhao Li,Xi Chen,Long Cheng,Yu Liu,Gongping Liu,Wanqin Jin
Carbon molecular sieve (CMS) membranes with exceptional separation performance and scalable processing are promising for precise gas separation. However, their broad applicability is hampered owing to stability issues, mainly resulting from physical aging. Herein, we manipulate the pyrolysis temperature (550 °C, 650 °C and 750 °C) to regulate the gas transport properties and control the physical aging of CMS membranes derived from 6FDA-DAM polyimide. The morphology, chemical composition and pore size of the membranes were characterized using SEM, IR, XPS and XRD. Results demonstrated that the pore structure of CMS membranes shows densification with increasing the pyrolysis temperature, affording enhanced separation efficiency for gas pairs: H 2 /N 2 , H 2 /CH 4 , and CO 2 /CH 4 pairs based on the size-sieving effect. Moreover, the effect of aging is more considerable for membranes pyrolyzed at a higher temperature. In addition, the membrane subjected to pre-aging treatment via vacuum storage exhibit better, stable gas separation performance beyond the upper-bond for polymeric membranes. The separation mechanism of the CMS membranes reveals that gas permeation behavior is dominated by diffusion. Tailoring gas permeation and physical aging can provide an alternative approach to tune the gas transport properties of CMS membranes.
研究领域:
材料科学
·
化学工程
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Cuiyuan Jin,Caiyun Wang,Chen Zhang,Qi Ye,Yuanxiang Jin,Liyun Shi
As a fungicide with the characteristics of high effectiveness, internal absorption and broad spectrum, imazalil is widely used to prevent and treat in fruits and vegetables. Here, pregnant C57BL/6 mice were exposed to imazalil at dietary levels of 0, 0.025‰, and 0.25‰ through drinking water during pregnancy and lactation. We then analyzed the phenotype, metabolome, and expression of related genes and proteins in the livers of mice. There was a marked decrease in the body and liver weights of male offspring mice after maternal imazalil exposure, while this effect on the dam and female offspring was slight. Metabolomics analyses revealed that imazalil significantly altered the metabolite composition of liver samples from both dams and offspring. The preliminary results of the analysis indicated that glucolipid metabolism was the pathway most significantly affected by imazalil. We performed a coabundance association analysis of metabolites with significant changes in the pathway of glycolipid metabolism, and IMZ altered the networks of both dams and offspring compared with the network in control mice, especially in male offspring. The hepatic triglyceride, non-esterified fatty acid and glucose levels were increased significantly in the dams but decreased significantly in male offspring after maternal imazalil exposure. Furthermore, the expression levels of genes associated with glycolipid metabolism and m 6 A RNA methylation were significantly affected by maternal intake of imazalil. Imazalil-induced glucolipid metabolism disturbance was highly correlated with m 6 A RNA methylation. In conclusion, maternal imazalil exposure resulted in glucolipid metabolism disturbance and abnormal m 6 A RNA methylation in the livers of dams and offspring mice. We expected that the information acquired in this study will provide novel evidence for understanding the effect of maternal imazalil exposure on potential health risks.
研究领域:
毒理学
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代谢组学
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分子生物学
·
发育生物学
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高影响因子
ACS Applied Engineering Materials
·
2026
·
IF 3.5
Zehui Yao,Xinhai Zhang,Yi Wang,Xiaozu Wang,Zhaohui Wang,Zhaoliang Cui
Polyimide (PI) membranes face a trade-off between permeability and selectivity, limiting their gas separation applications. To address this, we synthesized 6FDA-DAM/DABA(DAM:DABA = 3:2) PI and two-dimensional COF-1 nanofillers, fabricating hollow fiber mixed matrix membranes (MMMs) via dry-jet wet-spinning. Under optimized conditions (25 cm air gap, 50 °C spinneret temperature, 30 °C NMP coagulation bath), the 10 wt % COF-1 incorporated membranes achieved optimal performance: tensile strength of 15.34 MPa; H2 and CO2 permeances of 79.3 GPU and 54.3 GPU, and ideal selectivities of 187.9 for H2/CH4 and 128.7 for CO2/CH4, respectively. The results demonstrate that the incorporation of COF-1 modifies solvent exchange during spinning, necessitating targeted optimization of spinning parameters to coordinate membrane structure and performance.
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IMMUNITY
·
2024
·
IF 25.5
Yangning Lan,Xiaoxuan Zhang,Shaorui Liu,Chen Guo,Yuxiao Jin,Hui Li,Linyixiao Wang,Jinghong Zhao,Yilin Hao,Zhicheng Li,Zhaoyuan Liu,Florent Ginhoux,Qi Xie,Heping Xu,Jie-Min Jia,Danyang He
Microglial reactivity to injury and disease is emerging as a heterogeneous, dynamic, and crucial determinant in neurological disorders. However, the plasticity and fate of disease-associated microglia (DAM) remain largely unknown. We established a lineage tracing system, leveraging the expression dynamics of secreted phosphoprotein 1（Spp1） to label and track DAM-like microglia during brain injury and recovery. Fate mapping of Spp1 + microglia during stroke in juvenile mice revealed an irreversible state of DAM-like microglia that were ultimately eliminated from the injured brain. By contrast, DAM-like microglia in the neonatal stroke models exhibited high plasticity, regaining a homeostatic signature and integrating into the microglial network after recovery. Furthermore, neonatal injury had a lasting impact on microglia, rendering them intrinsically sensitized to subsequent immune challenges. Therefore, our findings highlight the plasticity and innate immune memory of neonatal microglia, shedding light on the fate of DAM-like microglia in various neuropathological conditions.
研究领域:
神经科学
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免疫学
·
细胞生物学
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高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jialin Zhang,Biyao Mao,Shuoshuo Cheng,Kangqiang Lu,Herui Wen,Jiali Ren
Assessing the activity of DNA methyltransferases (MTases) and screening for methyltransferase inhibitors not only allow for a deep exploration of the role of methylation regulation in disease initiation and progression but also provide an important experimental and clinical basis for the diagnosis and treatment of diseases. Herein, a new COFs functionalized electrochemical biosensor has been developed to detect DNA adenine methylation (Dam) MTase activity with high sensitivity and rapidity by taking advantage of the DNA walker and rolled circular strand displacement amplification (RC-SDA) reaction. Specifically, hairpin probe H1 was methylated by Dam MTase, followed by methylation site-specific cleavage of DpnI enzyme to generate the S5 probe. The padlock probes with two nucleic acid endonuclease sites were introduced to trigger the RC-SDA reaction under the action of the primer, releasing a large number of single-stranded S6 probes. The released probe worked synergistically with the Exo III enzyme to trigger DNA walking, which exposed the binding sites to enable the Au-COF-MB signal probes to bind effectively to the electrode surface, and the electrochemical signals were thus generated. The result showed that the designed electrochemical biosensor demonstrated excellent sensitivity and specificity in detecting the activity of Dam MTase, with the detection limit (LOD) of 6.85 × 10–5 U/mL. This method provides support for the development of new treatment strategies for methylation related diseases.
研究领域:
分子生物学
·
分析化学
·
医学诊断
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高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2022
·
IF 10.319
Huali Li,Liuqin Zhang,Xiaohu Zhang,Shuwen Luo,Baoshan Yang,Min Wu,Wei-Hua Li,Fa-Qian Liu
The antifouling of marine facilities placed below the waterline is a key constraint to the development of the marine economy. Currently, commercial Cu 2 O-based antifouling coatings still have defects such as agglomeration, oxidation, short duration and high dosage. For this reason, we developed for the first time a triple antifouling system consisting of Cu 2 O@poly(NIPAM-co-DAm) (Cu 2 O@PND) dynamic redox microgels triggered by copper ions and polydimethylsiloxane (PDMS). The catechol groups of PND nanogels can chelate Cu 2+ and reduce it in situ to obtain Cu 2 O@PND microgels, which guarantees the stable release of Cu + . Furthermore, Cu 2 O remains stable and free from oxidation because of the electron transfer between PND and Cu 2+ . Finally, superior antifouling performance was achieved by combining the stable Cu + released from Cu 2 O, in situ conversion of PND to hydrogel, and its oxidation to produce active substances and H 2 O 2 , and PDMS coating with low surface energy.
研究领域:
材料科学
·
化学
·
海洋工程
·
生物技术
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高影响因子
CERAMICS INTERNATIONAL
·
2021
·
IF 4.527
Cong Wu,Xinghui Wei,Kang Zhao,Junyi Jiang,Mengzhen Jiao,Junhua Cheng,Zhen Tang,Zheng Guo,Yufei Tang
Nanotube coatings on titanium surfaces have favorable biocompatibility and structures and are widely used in local drug delivery. However, when a drug freely diffuses out of a nanotube, it leads to an explosive release, making the duration of action extremely short. In this study, a high concentration of vancomycin hydrochloride region similar to a dam was formed on a nanotube coating surface by exploiting the attraction of the electric charge generated by the energy conversion of the piezoelectric effect to vancomycin hydrochloride. The “dam” not only reduces the diffusion rate of vancomycin hydrochloride inside the nanotube into the dam and the diffusion rate of the dam to the outside but also forms a high concentration of vancomycin hydrochloride action region. The free diffusion rate of vancomycin hydrochloride loaded on the TiO 2 @ BaTiO 3 coaxial nanotubes was reduced by the piezoelectric effect, and the cumulative release of vancomycin hydrochloride for 7 days is reduced by 54.8%. The polarized vancomycin-containing coaxial nanotube coating has a long-lasting antibacterial effect on Staphylococcus aureus . The TiO 2 @ BaTiO 3 coaxial nanotubes loaded with vancomycin hydrochloride coating with piezoelectric properties has potential application value in controlled drug delivery.
研究领域:
材料科学
·
生物医学工程
·
药学
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高影响因子
危险品化学品经营许可证（带存储）