MICROCHIMICA ACTA
·
2014
·Vol. 181
·期 15
·1981-1989
·
IF 3.719
He Linghao,Zhang Yuanchang,Liu Shunli,Fang Shaoming,Zhang Zhihong
We report on a novel graphene-based nanoarchitecture modified with plasma-polymerized propargylamine (G-PpPG) and its application in electrochemical sensors for DNA. Films of G-PpPG were characterized by X-ray photoelectron spectroscopy and electrochemical impedance spectroscopy. The presence of graphene enhances the electrochemical activity of the films, and the high density of amino groups (deposited at a low plasma input power) on their surface assists in the immobilization of probe DNA on the water-swollen polymeric network. By contrast, the degree of hybridization of the total complementary target DNA to the probe DNA remains unchanged when G-PpPG nanofilms prepared at higher input power. No substantial non-specific adsorption of totally mismatched target DNA on the polymer films is observed because of the complete coverage of the probe DNA. The detection limit for total complementary target DNA is approximately 1.84 nmol · L −1 . The dynamic range extends from 0.1 to 1,000 nmol · L −1 . The new nanocomposite may also be used to immobilize other probe DNA sequences, and this makes the approach potentially applicable to the detection of other oligomers.
Preparing the DNA sensor made from the graphene-based nanoarchitecture modified by using PpPG (G-PpPG) includes the following processes: (a) Modifying the Au electrode with the graphene nanosheet, (b) depositing the PpPG film onto the Au electrode coated with graphene, (c) immobilizing the probe DNA onto the G-PpPG film, and (d) hybridizing the MM0 target with the G-PpPG film immobilized with P1
研究领域:
生物传感
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材料科学
·
分子生物学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Dongli Chen,Meng Zhang,Mingyi Ma,Hong Hai,Jianping Li,Yang Shan
A novel electrochemical DNA biosensor was developed and MON89788 of soybean transgenic gene sequence was detected based on a strategy of rolling circle amplification (RCA) and gold nanoparticle cube (AuNPC)-labeled multiple probes. First, the mercapto-modified capture DNA was immobilized on the surface of the Fe 3 O 4 @Au magnetic nanoparticles via an Au–S bond, and the capture DNA was opened and complementarily hybridized with the target DNA to form a double-stranded DNA. In the 10 × reaction buffer, Exonuclease III (ExoIII) specifically recognized and sheared the double-stranded DNA to release the target DNA, which led to the next round of reaction. Afterward, AuNP cube-loaded ssDNA (AuNPC/DNA) was added with the rolling circle reaction with the help of Phi29 DNA polymerase and T4 ligase . Finally, [Ru(NH 3 ) 6 ] 3+ was attracted directly by the anionic phosphate of ssDNA via electrostatic interaction . The determination was carried out by using chronocoulometry (CC), and the CC signal was recorded. The mass amount of DNA strands extended infinitely on the AuNPs cube and numerous [Ru(NH 3 ) 6 ] 3+ were absorbed, thus the detected signal was highly amplified. The corresponding CC signal showed a good linear relationship with the logarithm of the target DNA concentration in the range of 1 × 10 −16 to 1 × 10 −7 mol L −1 , with a detection limit of 4.5 × 10 −17 mol L −1 . Specific gene sequence of MON89788 in soybean samples was determined, and the recoveries ranged from 97.3% to 102.0%. This sensor is one of the most sensitive sensors for genetic sequence assessment at present. Moreover, it demonstrates good selectivity , stability, and reproducibility.
研究领域:
生物技术
·
分子生物学
·
分析化学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Zhao Zhang,Liqiang Luo,Guifang Chen,Yaping Ding,Dongmei Deng,Chunhai Fan
A novel simple protocol of synthesizing tryptamine-functionalized reduced graphene oxide (TRA–rGO) was proposed to fabricate label-free electrochemical impedance DNA biosensor. TRA was anchored to rGO with its indole ring via π-stacking interaction. The morphology, conductivity and interaction of TRA–rGO were characterized by atomic force microscopy , high resolution transmission electron microscopy , cyclic voltammetry , electrochemical impedance spectroscopy (EIS) as well as Raman and fluorescence spectroscopy. The amino-substituted oligonucleotide probe was conjugated to TRA by cross-linker glutaraldehyde for preparing an electrochemical biosensing platform. The DNA hybridization reaction of oligonucleotide probe with target DNA was monitored by EIS. Under optimum conditions, the proposed biosensor exhibited high sensitivity and low detection limit for detecting complementary oligonucleotide. The target oligonucleotide could be quantified in a wide range of 1.0×10 −12 –1.0×10 −7 M with low detection limit of 5.2×10 −13 M ( S/N =3).
研究领域:
生物传感技术
·
材料科学
·
分子生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Minghua Wang,Shunli Liu,Yuanchang Zhang,Yanqin Yang,Yu Shi,Linghao He,Shaoming Fang,Zhihong Zhang
Despite the high toxicity of mercury (Hg), it has broad-ranging applications such as in thermometers and batteries. Consequently, serious environmental pollution is inevitable. In this study, we prepared graphene nanostructures used as highly sensitive and selective DNA sensors for the determination of Hg 2+ ions. The sensors consisted of multiple layers of nanostructured plasma-polymerized allylamine (PPAA) and nanosheets of conductive graphene. After immobilizing the probe DNA onto the sensitive layer and upon introducing the target analyte, Hg 2+ ions became intercalated into the DNA poly-ion complex membrane based on T-Hg 2+ -T coordination chemistry. Experimental results revealed that the frequency variation of the quartz chip of our system increased with increased Hg 2+ level in the sample and had limits of detection of 0.031 and 0.017 nM determined by QCM and electrochemical measurements, respectively. The strategy afforded high selectivity of Hg 2+ against other interfering metal ions. In addition, the developed DNA sensor for the determination of Hg 2+ ions could be reproduced up to 10 cycles at approximately 88% recovery.
研究领域:
生物传感技术
·
环境科学
·
材料科学
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DOI
高影响因子
TALANTA
·
2016
·
IF 4.035
Yuhong Liu,Linlin Zhao,Jin Zhang,Jinzha Zhang,Wenbo Zhao,Chun Mao
The work investigates a new fluorescence resonance energy transfer (FRET) system using NaEuF 4 nanoparticles (NPs) and [email protected] 2 S NPs as the energy donor–acceptor pair for the first time. The NaEuF 4 / [email protected] 2 S NPs-based FRET DNA sensor was constructed with NaEuF 4 NPs as the fluorescence (FL) donor and [email protected] 2 S core−shell NPs as FL acceptor. In order to find the matching energy acceptor, the amount of AgNO 3 and Na 2 S were controlled in the synthesis process to overlap the absorption spectrum of energy acceptor with the emission spectrum of energy donors. The sensitivity of FRET-based DNA sensor can be enhanced and the self-absorption of ligand as well as the background of signals can be decreased because of Eu 3+ which owns large Stokes shifts and narrow emission bands due to f–f electronic transitions of 4f shell. We obtained the efficient FRET system by studying suitable distance between the donor and acceptor. Then the FRET-based DNA sensor was used for the design of specific and sensitive detection of target DNA and the quenching efficiency (ΔFL/F 0 , ΔFL=F−F 0 ) of FL was logarithmically related to the concentration of the target DNA, ranging from 100 aM to 100 pM. We can realize an ultrasensitive detection of target DNA with a detection limit of 32 aM. This proposed method was feasible to analyse target DNA in real samples with satisfactory results.
研究领域:
生物化学
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分子生物学
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纳米技术
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DOI
PubMed
高影响因子
Journal of the American Chemical Society
·
2009
·
IF 8.091
Yinghua Peng,Xiaohui Wang,Yi Xiao,Lingyan Feng,Chao Zhao,Jinsong Ren,Xiaogang Qu
The increasing worldwide demand for carbon nanotubes (CNTs) and increasing concern regarding how to safely develop and use CNTs are requiring a low-cost, simple, and highly sensitive CNT detection assay for toxicological evaluation and environmental monitoring. However, this goal is still far from being achieved. All the current CNT detection techniques are not applicable for automation and field analysis because they are dependent on highly expensive special instruments and complicated sample preparation. On the basis of the capability of single-walled carbon nanotubes (SWNTs) to specifically induce human telomeric i-motif formation, we design an electrochemical DNA (E-DNA) sensor that can distinguish single- and multiwalled carbon nanotubes both in buffer and in cell extracts. The E-DNA sensor can selectively detect SWNTs with a direct detection limit of 0.2 ppm and has been demonstrated in cancer cell extracts. To the best of our knowledge, this is the first demonstration of a biosensing technique that can distinguish different types of nanotubes. Our work will provide new insights into how to design a biosensor for detection of carbon nanotubes.
研究领域:
生物传感
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环境监测
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毒理学
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DOI
PubMed
高影响因子
ANALYST
·
2015
·Vol. 140
·期 19
·6660-6670
·
IF 4.107
Qing Zheng,Hao Wu,Zongxu Shen,Wenyu Gao,Yu Yu,Yuehui Ma,Weijun Guang,Quangui Guo,Rui Yan,Junzhong Wang,Kejian Ding
A label-free electrochemical DNA sensor was fabricated by deposition of polyaniline and pristine graphene nanosheet (P/Gratios) composites in different mass ratios, DNA probe and bovine serum albumin (BSA) layer by layer on the surface of a glassy carbon electrode (GCE). Electrochemical impedance spectroscopy (EIS) was employed to monitor every step of fabrication of P/Gratio-based DNA sensors and to evaluate the detection results in terms of the hybridization of complementary DNA, mutant DNA and non-complementary DNA. The results illustrate that the P/Gratio-based DNA sensor could highly efficiently detect complementary DNA from 0.01 pm to 1 μm and discriminate single-nucleotide polymorphisms (SNPs). In the process of detection, double-stranded DNA (dsDNA), resulting from hybridization of a DNA probe, escaping from or remaining on the sensor surface, was monitored by changing the ratio of polyaniline (PANI) to graphene, which was decided by the competition between the electrostatic interaction and Brownian motion.
研究领域:
生物化学
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纳米材料科学
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分子诊断学
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 49
·43501-43508
·
IF 3.289
Hongzhi Wang,Yu Wang,Su Liu,Jinghua Yu,Yuna Guo,Ying Xu,Jiadong Huang
In this work, a novel electrochemical DNA sensor based on exonuclease III (Exo III)-assisted autocatalytic DNA biosensing platform for ultrasensitive detection of antibiotics has been reported. When the arched probe was challenged with target, the released primer caused by the specific recognition of the aptamer and target antibiotics hybridized with the 3′-protruding terminus in the HP1. Then, Exo III could catalyze the stepwise removal of mononucleotides from this flat terminus, resulting in the release of the primer and trigger. As a secondary target, trigger could replace Helper from the 5′-MB labeled HP2, and HP2 formed a “close” probe structure, confining MB close to the electrode surface for efficient electron transfer. To our best knowledge, such work is the first report about Exo III-assisted autocatalytic DNA biosensing platform combing with signal-on sensing strategy, which has been utilized for quantitative determination of antibiotics. It would be further used as a general strategy associated with more analytical techniques toward the detection of a wide spectrum of analytes. Thus, it holds great potential for the development of ultrasensitive biosensing platform for the applications in bioanalysis, disease diagnostics, and clinical biomedicine.
研究领域:
生物分析
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分子诊断
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临床生物医学
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DOI
高影响因子
ELECTROANALYSIS
·
2022
·Vol. 35
·期 4
·e202200330
·
IF 3.077
Ting Huang,Wenxing Zhang,Guobing Wie,Nian Hong,Mei Wang,Qiqi Fan,Qiangqiang Yu,Fusheng Liao,Hao Fan
Herein, a reagentless electrochemical DNA sensor based on a self-powered DNA machine for detecting survivin mRNA in cells is developed. The metal-organic framework (MOFs) loaded with DNAzyme cofactors (Mn 2+ ) is coated on PTFE rods on the Au surface and assembled with the DNA walker, overcoming the complexity of adding metal ions from the external environment. In addition, the orbital chain is modified by a synthetic bisferrocene signal marker, which further enables signal amplification. Under optimal conditions, the sensor exhibits a range from 1×10 −14 mol/L to 1×10 −8 mol/L with a detection limit (S/N=3) of 1.28 fM.
研究领域:
生物传感技术
·
分子生物学
·
纳米材料科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Wenting Wang,Xiaojian Fan,Shenghao Xu,Jason J. Davis,Xiliang Luo
A label-free and low fouling biosensor based on functional polyethylene glycols selective for breast cancer susceptibility gene (BRCA1) is reported. Sensory interfaces were prepared through the modification of a glassy carbon electrode with highly cross-linked polyethylene glycol (PEG) film containing amine groups, followed by the self-assembly of gold nanoparticles and the immobilization of BRCA1 complementary single-strand 19-mer oligonucleotides . In the presence of a specific BRCA1 sequence capture and hybridization results in interfacial change sensitively monitored using electrochemical impedance spectroscopy. The combined utilization of a PEG polymer film and gold nanoparticle mixed interface enables very high levels of sensitivity and a highly effective assaying in patient samples. Assay linear range was from 50.0 fM to 1.0 nM, with a limit of detection of 1.72 fM. Furthermore, this label-free DNA sensor has been used for assaying BRCA1 in serum samples, showing its feasible potential for diagnostic applications in clinical analysis of breast cancer gene BRCA1. Foreseeable, this sensor made on this basis undoubtedly provide the most effective and sensitive detection for BRCA1.
研究领域:
生物传感器
·
癌症诊断
·
纳米技术
·
电化学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Shaoguang Li,Hongxing Li,Xun Li,Man Zhu,Hui Li,Fan Xia
Hybridization chain reaction (HCR) amplification strategy has been extensively explored for the application of electrochemical DNA-based sensors. Despite the enhancement in its sensitivity using the HCR, such sensor platform exhibited significant sensor-to-sensor variations in current due to variations in probe counts and lengths. To circumvent this, we are developing here a calibration-free “O-N” approach to generate a ratiometric, unitless value that is independent of these variations. Specifically, this approach employs two types of redox reporters, denoted as “One reporter” and “N reporters”, with the former attached on the capture DNA and the latter on H1 and H2 strands. By optimizing the attachment sites of these reporters onto DNA strands, we demonstrate a significantly enhanced sensitivity of such sensor platform by four orders of magnitude, achieving accurate, calibration-free measurement of nucleic acids including ctDNA directly in undiluted whole blood without the requirement to calibrate each individual sensor.
研究领域:
生物化学
·
分析化学
·
分子生物学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Guixiang Wang,Rui Han,Xiaoli Su,Yinan Li,Guiyun Xu,Xiliang Luo
Zwitterionic peptides were anchored to a conducting polymer of citrate doped poly(3,4-ethylenedioxythiophene) (PEDOT) via the nickel cation coordination, and the obtained peptide modified PEDOT, with excellent antifouling ability and good conductivity , was further used for the immobilization of a DNA probe to construct an electrochemical biosensor for the breast cancer marker BRCA1 . The DNA biosensor was highly sensitive (with detection limit of 0.03 fM) and selective, and it was able to detect BRCA1 in 5% (v/v) human plasma with satisfying accuracy and low fouling. The marriage of antifouling and biocompatible peptides with conducting polymers opened a new avenue to construct electrochemical biosensors capable of assaying targets in complex biological media with high sensitivity and without biofouling.
研究领域:
生物传感器
·
电化学
·
材料科学
·
癌症研究
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）