JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2018
·
IF 3.235
Hong Zhang,Zhaopeng Song,Feilong Pan,Fan He
A novel and enzyme-free electrochemical sensor was designed for multiplexed DNA detection based on a surface-confined DNA assembly enabled target recycling amplification. In this designed system, two hairpin DNA probes (hairpin 1 and hairpin 2) were immobilized on the surface of gold nanoparticles/graphene/nafion modified glassy carbon electrode (AuNPs/Gr/Nf/GCE). In the presence of two DNA targets, the hairpin structures of hairpin 1 and hairpin 2 opened through the complementary hybridization, resulting in the formation of hairpin 1: target DNA 1 and hairpin 2: target DNA 2. When they met methylene blue (MB)-labeled hairpin 3 (hairpin 3-MB) and ferrocene (Fc)-labeled hairpin 4 (hairpin 4-Fc), DNA cyclic amplification was activated, and the DNA targets were released and could be reused for the next recycling. Meanwhile, coupled with the utilization of positively charged gold nanoparticles ((+)AuNPs), multiplexed DNA detection was realized in the concentration range from 10 fM to 1.5 pM with the detection limits of 3.6 fM and 2.5 fM for target DNA 1 and target DNA 2, respectively. Moreover, satisfactory results were obtained when detecting DNA targets in human serum samples, suggesting that this proposed sensing platform is promising for the applications in complex biological samples.
研究领域:
生物化学
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分析化学
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纳米技术
·
分子生物学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Ting Wang,Rong-Fu Huang,Zeqin Chen
Development of simple, sensitive and rapid sensing strategy for DNA damage induced by chemical reagents is urgent demand. In this assay, a Tb 3+ /G-quadruplex fluorescence system was proposed for the sensitive detection of DNA damage induced by Fenton reaction. The specific guanine-rich oligonucleotides can form G-quadruplex induced by Tb 3+ , leading to an enhancement of Tb 3+ emission due to energy transfer from guanine to Tb 3+ . After a DNA damage reaction with Fenton reagents, an obvious fluorescence decrease was observed due to the structure change of Tb 3+ /G-quadruplex. which was also confirmed by polyacrylamide gel electrophoresis and circular dichroism. The Fe 2+ -mediated Fenton reaction with as low as 0.01 μM Fe 2+ and 0.04 μM H 2 O 2 induced oxidative DNA damage can be detected. Additionally, the human 8-oxoguanine DNA glycosylase (hOGG 1) was introduced to amplify DNA oxidation damage, by converting DNA oxidative nucleobases to strand break. Over 30-fold lower detection limit of 0.3 nM Fe 2+ and 1.2 nM H 2 O 2 was achieved. Alternative specific DNA damage or other DNA repair enzymes produced much less fluorescence decrease, demonstrating its good selectivity. It shows the potential to become a universal screening tool for the rapid and sensitive assessment of the genotoxicity of the overwhelming number of chemicals in environment.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
高影响因子
Materials Horizons
·
2018
·Vol. 6
·期 2
·375-384
·
IF 13.183
Daoqing Fan,Erkang Wang,Shaojun Dong
DNA computing has shown impressive developments and exhibited amazing power over the past few decades. However, most current DNA logic devices can only produce single-modal output that is monochromic or even invisible, which greatly limits their reliability and practicality, integration capability and computing complexity. Emission-tunable upconversion nanoparticles (UCNPs) are ideal multicolor outputs for DNA computing, however UC luminescent (UCL) DNA logic systems have rarely been reported. Here, for the first time, we report “DNA-Unlocked Inner-Filter-Effect” (DU-IFE) between oxidized 3,3′,5,5′-tetramethylbenzidine (OxTMB) and upconversion nanoparticles (UCNPs) that subtly matches a “lock–key” strategy. Based on the DU-IFE, we construct the first ever UCL DNA logic library. Superior to previous DNA logic systems, this logic library can generate dual-modal (visual and UCL) multicolor label-free outputs, in which RGB (red, green, blue) colorful UCL ones are also visual under harmless NIR-laser. By selectively unlocking the IFE “lock” with different DNA “keys”, diverse basic, advanced and concatenated logic circuits, a logic-controlled molecular RGB-signal-light, triple-parallel-arrays are successfully constructed. All of the above logic operations demonstrate the strengthened reliability and practicality, powerful integration capability and improved computing complexity of this UCL logic library. Moreover, we fabricate an inspiring and useful biosensor with RGB colorful results based on the DU-IFE (a DNA caliper), providing a vivid prototype for potential biological applications.
研究领域:
生物化学
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分子生物学
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纳米技术
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生物医学工程
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
·
IF 7.7
Ting Li,Shaojiao Song,Wei Lu,Yu Cao,Xin Zheng,Qiuyue Zhao,Hailong Wang
Owing to the high selectivity and binding affinity of coralyne (COR) with deoxyribonucleic acid (DNA)/ribonucleic acid, COR has been confirmed as an effective anticancer drug. However, a direct and sensitive detection assay for a DNA–COR–DNA structure with a portable instrument has not been reported because of the lack of a high-resolution sensor that can identify DNA–COR interaction at a single-molecule level. Here, specific Types I and II signals generated by a DNA–COR–DNA duplex were observed using a versatile α-hemolysin nanopore sensor. The Type I signal featuring a unique current pattern was preferentially selected via DNA sequence design and voltage control. Subsequently, the rapid and sensitive detection of COR was established with a detection limit of 0.1 nM. The pH environment, observed to influence the binding of the DNA–COR–DNA duplex, was significant for drug release controlling during targeted delivery process. Similar alkaloids were tested, and the nanopore sensor exhibited a high selectivity for COR. The results demonstrate a potential platform for investigating DNA-COR interaction and could be applied in the drug therapy and pharmacodynamic studies of COR.
研究领域:
生物物理学
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分子生物学
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药理学
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DOI
高影响因子
VIROLOGY
·
2023
·
IF 3.7
Xiaoping Huang,Xiao Wang,Yaqi Sun,Ling Li,Anna Li,Wentao Xu,Xiaolan Xie,Yong Diao
Because it is safe and has a simple genome, recombinant adeno-associated virus (rAAV) is an extremely appealing vector for delivery in in vivo gene therapy. However, its low transduction efficiency for some cells, limits its further application in the field of gene therapy. Bleomycin is a chemotherapeutic agent approved by the FDA whose effect on rAAV transduction has not been studied. In this study, we systematically investigated the effect of Bleomycin on the second-strand synthesis and used CRISPR/CAS9 and RNAi methods to understand the effects of Bleomycin on rAAV vector transduction, particularly the effect of DNA repair enzymes. The results showed that Bleomycin could promote rAAV2 transduction both in vivo and in vitro. Increased transduction was discovered to be a direct result of decreased cytoplasmic rAAV particle degradation and increased second-strand synthesis. TDP1 , PNKP , and SETMAR are required to repair the DNA damage gap caused by Bleomycin, TDP1, PNKP, and SETMAR promote rAAV second-strand synthesis. Bleomycin induced DNA-PKcs phosphorylation and phosphorylated DNA-PKcs and Artemis promoted second-strand synthesis. The current study identifies an effective method for increasing the capability and scope of in-vivo and in-vitro rAAV applications, which can amplify cell transduction at Bleomycin concentrations. It also supplies information on combining tumor gene therapy with chemotherapy.
研究领域:
分子生物学
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基因治疗
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癌症研究
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药理学
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DOI
PubMed
高影响因子
Nanoscale
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2018
·Vol. 10
·期 12
·5532-5538
·
IF 7.233
Bi-Yun Fang,Cheng Li,Jie An,Sun-Duo Zhao,Zhi-Yong Zhuang,Yuan-Di Zhao,Yue-Xuan Zhang
In this paper, DNA containing six cytosines as the formation site for silver nanoclusters (Ag NCs) was adopted as a template for preparing fluorescent DNA-Ag NCs. For the first time, it was found that the fluorescence of DNA-Ag NCs could be quenched after hybridization with their complementary sequence. On the basis of this new phenomenon, we designed a sequence C1 that was completely complementary to human immunodeficiency virus (HIV) DNA, and probe DNA which was partially complementary to C1 for the synthesis of DNA-Ag NCs. The fluorescence of DNA-Ag NCs was quenched after hybridization with C1 and the DNA-Ag NCs/C1 composite was formed, while C1 could be dissociated away from the DNA-Ag NCs by HIV DNA through a strand exchange reaction due to the stronger affinity between HIV DNA and C1, which could switch on the quenched Ag NCs, thus a new “off–on” fluorescence method for HIV DNA detection was developed. In the experiment, the Ag NCs formation site of DNA, the number of base pairs, and the pH and salt concentration of binding buffer were optimized. Under the optimum conditions, the limit of detection for HIV DNA was obtained to be 3.18 nM (3σ/N, n = 7) with the linear range of 15–150 nM for the 150 nM DNA-Ag NCs/C1 probe. Besides, the probe showed excellent specificity to HIV DNA, and even distinguished one nucleotide mismatched HIV DNA.
研究领域:
生物技术
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纳米技术
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分子生物学
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病毒学
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产品关联
DOI
PubMed
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2018
·Vol. 32
·期 4
·e4231
·
IF 3.581
Xue-Wen Liu,Yang Xiao,Wei-Gen Peng,Li-Jin Zhao,You-Ming Shen,Song-Bai Zhang,Ji-Lin Lu
Two ruthenium complexes containing a new ligand phipz (phipz = 2-(1,10-phenanthroline)-1H-imidazo[4,5-b]phenazine) were designed and synthesized. These complexes were found to inhibit the DNA supercoiled relaxation mediated by topoisomerase I (topo I), cleave DNA under irradiation and bind to calf thymus DNA through intercalative mode. Furthermore, complex 2 shows higher photocleavage activity, topo I inhibition activity and DNA affinity than complex 1 . Additionally, introduction of phenazine unit may be the reason that two complexes exhibit DNA ‘light switch’ behavior. The present work shows that two complexes might be potential as new DNA ‘light switches’, DNA photocleavers and topo I inhibitors.
研究领域:
化学
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生物化学
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分子生物学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2023
·
IF 7.4
Baoting Dou,Hui Zhou,Xiguang Han,Po Wang
The dissociation of the walking strand from the track gives rise to decreased efficiency and long reaction time of DNA walkers. In this work, we constructed a DNA walker combining the introduction of a wedge segment with a bimetallic metal–organic framework (MOF) electrocatalyst to solve this problem. The target methylated DNA acted as a single-legged walker, and the immobilization probe assembled on the track contained a wedge segment that was complementary to the target methylated DNA persistently, inhibiting its dissociation from the track. The fuel strand modified with a bimetallic MOF would drive the target strand to conduct branch migration and move processively along the track. The stepwise movement of the target strand resulted in the loading of numerous bimetallic MOF catalysts to reduce H2O2 at the electrode interface, thereby a significantly increased current response would be obtained for the detection of methylated DNA. This DNA walker achieved a detection limit of 200 aM within 20 min and effectively distinguished DNA with different methylation statuses, which would pave a way for rapid and sensitive monitoring of DNA methylation.
研究领域:
生物化学
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2022
·Vol. 35
·期 4
·e202200330
·
IF 3.077
Ting Huang,Wenxing Zhang,Guobing Wie,Nian Hong,Mei Wang,Qiqi Fan,Qiangqiang Yu,Fusheng Liao,Hao Fan
Herein, a reagentless electrochemical DNA sensor based on a self-powered DNA machine for detecting survivin mRNA in cells is developed. The metal-organic framework (MOFs) loaded with DNAzyme cofactors (Mn 2+ ) is coated on PTFE rods on the Au surface and assembled with the DNA walker, overcoming the complexity of adding metal ions from the external environment. In addition, the orbital chain is modified by a synthetic bisferrocene signal marker, which further enables signal amplification. Under optimal conditions, the sensor exhibits a range from 1×10 −14 mol/L to 1×10 −8 mol/L with a detection limit (S/N=3) of 1.28 fM.
研究领域:
生物传感技术
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分子生物学
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纳米材料科学
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产品关联
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 36
·3598-3604
·
IF 3.532
Wenxiu Huang,Lizhen Zhao,Ruidi Shen,Gongke Li,Liansheng Ling
A highly sensitive and selective RGB color analysis for the detection of formaldehyde (FA) was developed by using a DNA functionalized gold nanoparticle (AuNPs-DNA) probe. When complementary oligonucleotides (oligo 2 and oligo 3) and a silver ion (Ag+) were added to the AuNPs-DNA solution, triplex DNA was formed, resulting in the aggregation of AuNPs, and accompanied by a solution color change from red to purple. With the addition of formaldehyde, it reacted with Ag+, decreased the stability of triplex DNA between AuNPs–DNA, induced the dispersion of AuNPs, and the color of AuNPs recovered to red. Therefore, the formaldehyde concentration could be estimated with the RGB (red, green, blue) values of the AuNP solution by using a smartphone application (APP). The R value of the system was proportional to the concentration of formaldehyde within the range of 0.23–4.50 mg L−1, with a detection limit of 0.14 mg L−1. The method has been successfully applied to detect the residues of formaldehyde in vegetable samples and has the potential of the on-site determination of formaldehyde.
研究领域:
生物化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2015
·Vol. 162
·期 12
·B291
·
IF 3.266
Feng Gao,Xili Cai,Hidekazu Tanaka,Qionghua Zhu,Fei Gao,Qingxiang Wang
An ultrasensitive DNA biosensor was constructed in this work by using graphene oxide nanosheets (GONs) as the multi-site platform for probe DNA immobilization. First, L-cysteine (L-cys) was self-assembled on a gold electrode surface based on the Au-S chemistry. Then the GONs and amino modified probe DNA was covalently grafted to L-cys/AuE surface in turn, with the aid of 1-(3-Dimethylanminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxy succinimide (NHS). The layer upon layer assembly process was characterized with atomic force microscopy and electrochemical methods. Due to the nano-size effect and multiple site characteristic of GONs, the immobilization density of the probe DNA was determined to be as high as 5.72 × 10−10 mol cm−2 using methylene blue as the redox probe. The hybridization experiments showed that the biosensor can quantitatively detect target DNA in a wide range from 1.0 × 10−15 M to 1.0 × 10−9 M with a detection limit of 5.0 × 10−16 M. The selectivity experiments showed that the sensing system could accurately discriminate the complementary sequence from the base mismatched and non-complementary sequences.
研究领域:
生物技术
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纳米生物学
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分析化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Ding Wang,Yaqin Chai,Yali Yuan,Ruo Yuan
In this work, a rigid DNA tetrahedron (TDN) scaffold was synthesized to precisely control the interenzyme distance by randomly anchoring two pairs of horseradish peroxidase (HRP)/glucose oxidase (GOx) at the vertices of TDN, which could not only avoid the drawbacks of poor controllability and biocompatibility from traditional scleroid skeletons, but also overcome the defect of imprecise regulation for interenzyme distance caused by DNA origami. Impressively, by varying the side length of TDN scaffold, the interenzyme distance was precisely regulated, thus, an optimal TDN scaffold with highest catalytic efficiency was acquired and subsequently applied for constructing an ultrasensitive biosensor for DNA detection with a low detection limit of 3 fM. This strategy paved an avenue for developing new reliable scaffold to precisely regulate the catalytic efficiency of enzyme cascade reaction with ultimate applications in bioanalysis and clinical diagnosis.
研究领域:
生物化学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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