Forests
·
2024
·Vol. 15
·期 2
·236
·
IF 2.4
Liping Yu,Wang Cheng,Meifen Tian,Zhigang Wu,Xiaoli Wei,Xing Cheng,Mingwei Yang,Xuan Ma
The medicinal value ofP. zhennanhas been documented in traditional Chinese medicine books. The aim of this paper was to study the antioxidant activity of alcoholic extracts ofP. zhennanleavesusing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-phenyl-4,4,5,5-tetramethylimidazolineoxyl-1-oxyl-3-oxide (PTIO) radical scavenging and ferric ion reducing antioxidant power (FRAP) assays. The active components of the leaves were identified via headspace solid-phase microextraction and gas chromatography–mass spectrometry (HS-SPME-GC-MS). The results showed that the scavenging rate of DPPH was 94.67%with an EC50 value of 0.674 mg/mL at a concentration of 2 mg/mL. The maximum scavenging rate was 47.40% at a Trolox equivalent of 0.33 mg TE/mL for PTIO radicals. The FRAP reached 84.80% at 0.20 mg/mL concentration. The results confirmed the strong antioxidant activity of the extracts. Furthermore, 44 compounds, mostly terpenoids, obtained from the alcoholic extracts ofP. zhennanleaves were analyzed using HS-SPME-GC-MS and 15 of these compounds had a relative content exceeding 1%. The strong antioxidant activity of the alcoholic extracts ofP. zhennanleaves could be attributed to the presence of copaene (33.97%), β-caryophyllene (4.42%), δ-cadinene (11.04%), γ-muurolene (4.78%), cis-calamenene (2.02%), linalool (1.04%), α-pinene (1.46%), borneol acetate (1.5%), and γ-terpinene (0.66%). This study demonstrates the potential medicinal value of alcoholic extracts ofP. zhennanleaves.
研究领域:
植物学
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药理学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Evidence-based Complementary and Alternative Medicine
·
2013
·
IF 1.722
Huiqing Lv,Xiaoping Zhang,XueZhi Chen,Zhijun Xie,Changfeng Hu,Chengping Wen,Kezhi Jiang
Background. Ficus pandurata H. (Moraceae) is widely used in traditional Chinese medicine as a healthy food condiment or a medicine for treatment of various diseases including inflammation. Objective. The purpose of the present study is to investigate the phytochemical compositions and antioxidant and anti-inflammatory activities of crude water (FPW) and ethanolic extracts (FPE) from Ficus pandurata H. Methods. Phytochemical compositions were identified by a high-performance liquid chromatography-electrospray ionization-mass spectrometry method (HPLC-ESI-MS). The antioxidant activities were evaluated by diphenylpicrylhydrazyl (DPPH) and hydroxyl radical assays, and the anti-inflammatory activities were evaluated by paw edema and levels of inflammatory mediator TNF-α and PGE2 in monosodium urate (MSU) crystal-induced rats. Results. Six compounds were identified by HPLC-MS method, and abundance of phenolics was found in FPE. The FPE showed concentration-dependent-significant scavenging of DPPH and hydroxyl radicals with IC50 values 118.4 and 192.9 μg/mL, respectively. The FPE treatment significantly inhibited the paw edema and the production of TNF-α and PGE2 in MSU crystal-induced rats. Conclusion. The FPE exerted stronger antioxidant and anti-inflammatory activities which may be attributed to its high phenolic content.
研究领域:
药理学
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植物化学
·
传统医学
✓ 已验证
DOI
PubMed
高影响因子
FITOTERAPIA
·
2024
·
IF 2.5
Jiaqi Huang,Mengyao Wang,Yu Wang,He Sun,Yadan Zou,Xiaojin Tian,Dianxin Cui,Peng Zhang,Xue Li,Wenzhi Yang
Prunella vulgaris is a medicinal and edible homologous plant, commonly used as a folk medicine to treat diseases. The Prunella vulgaris polysaccharides (PVPs) are reported with the antioxidant activity. This work was designed to isolate, characterize, and test the antioxidant activity of purified PVPs from P. vulgaris . A new homogeneous polysaccharide (PVP-1) was prepared by the DEAE column from PVPs, and diverse chromatography/spectroscopy and chemical methods were simultaneously employed to characterize the fine structure of PVP-1. The results showed PVP-1 had a triple helix structure, and the repeating structural unit of PVP-1 was composed of →6)- β -D-Gal p -(1→6)- β -D-Gal p -(3,1→6)- β -D-Gal p -(1→6)- β -D - Gal p -(1→ as the main chain, together with →6)- β -D-Gal p -(1,3→1)- α -D-Ara f -(5→1)- β -D-Gal p -(4→1)- α -D-Gal p -(2→ and →6)- β -D-Gal p -(1,3→1)- α -D-GlcA p -(4→1)- α -D-Glc p -(4→1)- α -D-Gal p as the branch chains. The main monosaccharides of PVP-1 were galactose (Gal, 41.25 %), galactose-OMe (Gal-OMe, 27.73 %), arabinose (Ara, 10.63 %), mannose (Man, 9.86 %), glucose (Glc, 3.88 %), glucuronic acid (GlcA, 2.86 %), ribose (Rib, 1.79 %), and xylose (Xyl, 1.76 %). In addition, the scanning electron microscopy (SEM) displayed that the surface of PVP-1 was rough and porous. PVP-1 gave the scavenging rates of the DPPH, ABTS, and hydroxyl radical lower than vitamin C at the same concentration, with the highest scavenging rate of DPPH radical at 82.71 % ± 4.19 % (5 mg/mL).
研究领域:
植物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2022
·
IF 3.388
Duo Liu,Zenghui Shi,Siqi Wang,Liyuan Zhai,Dongxia Gou,Jun Zhao,Yanbo Hu
Black soybean hull, as main by-production in black bean processing, has significant biological activity. In this study, the polysaccharide from black soybean hull (BSPS-H) was obtained by enzymatic hydrolysis, the yield was 5.3%, and its chemical composition and structural composition were further studied. The results shown that BSPS-H was mainly composed of Mannose, Galactose, and Glucose (molar ratio = 48.1: 29.7: 15.8), and might be existed in the form of pyran ring in α/β-linkage type. The anti-oxidant activity shown that BSPS-H had a certain capacity of anti-oxidation in a concentration-dependent manner. Among them, it has the best scavenging effect on DPPH free radicals. When the polysaccharide concentration was 10 mg/ml, the scavenging rate reached 94.37%. The results of anti-fatigue effect indicated that BSPS-H could prolong the duration of exhaustive swimming in mice, increased the contents of liver glycogen, reduced the content of blood urea nitrogen (BUN), enhanced the activity of lactate dehydrogenase (LDH) and reduced the accumulation of lactic acid (LA). This study provides a fundamental basis for the development and use of BSPS-H in medicine and functional foods.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 9
·3928
·
IF 4.6
Jiao Huang,Donglin Chen,Mengying Liu,Yarui Yu,Yi Zhang,Jing Huang
Seven new phenylhexanoids, (S)-(+)-3,4-dihydroxy-11-methoxyphenylhex-9-one (1), (E) 3,4-dihydroxy-phenylhex-10-en-9-one (2), (E)-4-hydroxyphenylhex-10-en-9-one (3), (R)-(−)-3,4,11-trihydroxyphenylhex-9-one 11-O-β-d-glucopyranoside (4), (R)-(−)-4,11-dihydroxyphenylhex-9-one 11-O-β-d-glucopyranoside (5), phenylhex-4,9,11-triol 11-O-β-d-glucopyranoside (6), and 9-O-acetyl-phenylhex-4,9,11-triol 11-O-β-d-glucopyranoside (7), were isolated and identified from Tibetan medicineSaxifraga umbellulatavar.pectinate. The antioxidant activities of these compounds were evaluated using the DPPH and ABTS radical scavenging experiments. In the ABTS experiment, compounds1(IC5013.99 ± 2.53 μM) and2(IC5013.11 ± 0.94 μM) exhibited significantly better antioxidant activity than L-ascorbic acid (IC5023.51 ± 0.44 μM).Keywords:Saxifraga umbellulatavar.pectinate;phenylhexanoid;antioxidant activity
研究领域:
药用植物学
·
天然产物化学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Natural Product Communications
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2014
·
IF 0.924
Zihao Zhou,Jun Tang,Xueping Song
The chemical composition of the essential oil from roots and rhizomes of Ligularia hodgsonii was analyzed by gas chromatography (GC) and GC-mass spectrometry (MS). Forty-seven components were identified, representing 77.0% of the total oil. The main constituents were l-(+)-ascorbic acid 2,6-dihexadecanoate (15.7%), selina-6-en-4-ol (8.4%) and 9,10-dimethyl-1,2,3,4,5,6,7,8-octahydroanthracene (6.6%). The free radical scavenging activities of the essential oil, aqueous extract, ethanolic extract, and crude polysaccharides of L. hodgsonii, as well as some of their major components, were investigated using DPPH and ABTS assays. The essential oil displayed a lower capacity to quench free radicals than the extracts; the ethanolic and aqueous extracts showed considerably higher antioxidant potential that deserves further study.
研究领域:
植物化学
·
药理学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Zaheer Abbas,Jing Zhang,Yucui Tong,Baseer Ahmad,Junyong Wang,Haosen Zhang,Zhenzhen Li,Xubiao Wei,Rijun Zhang,Dayong Si
The objective of this study was to isolate and characterize antioxidant peptides from mulberry leaves fermented with Bacillus subtilis H4 and Bacillus amyloliquefaciens LFB112. The results indicated that fraction F4 (<1 kDa) exhibited superior DPPH activity compared to the F1 (>10 kDa), F2 (3–10 kDa), and F3 (1–3 kDa) fractions, and cytoprotective effect against lipopolysaccharides (LPS)-induced oxidative stress in RAW264.7 cells. Three novel peptides, FRFDP, RFGG, and GPPLAFGGGP, were identified in the F4 fraction of the mulberry leaf ferment, and docking results showed that these peptides could form stable carbon (covalent) and hydrogen bonds to the active sites of Keap1, thus regulating the Keap1-Nrf2 pathway by blocking the Nrf2 binding sites on Keap1. These peptides significantly upregulated the mRNA expression of Nrf2 , HO-1 , and NQO1 , providing indirect evidence of their potential to enhance cellular antioxidant defense via the possible activation of Keap1-Nrf2 pathway. Furthermore, these peptides showed good DPPH and ABTS scavenging activities, indicating their potential as antioxidant peptides. This study offers valuable insights into the integration of these novel peptides in developing mulberry leaf ferment as a functional food and their potential use as a feed additive.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMISTRY OF NATURAL COMPOUNDS
·
2020
·Vol. 56
·期 5
·821-826
·
IF 0.653
Shao Jin,Zhao Tong,Ma Hui-Ping,Jia Zheng-Ping,Jing Lin-Lin
A convergent synthesis route of 6-hydroxygenistein (6-OHG) was reported, starting from cheap and readily available biochanin A, via methylation, bromination, methoxylation, and demethylation. The structure of the products was confirmed by MS, IR, 1 H NMR, and 13 C NMR analysis. The antiradical activity of 6-OHG was determined using six different methods, namely, DPPH assay, ABTS assay, nitric oxide assay, superoxide assay, reducing power assay, and phosphomolybdenum assay using ascorbic acid (V C ) as positive control. The results show that 6-OHG possesses similar or greater antiradical activity than V C . In conclusion, 6-OHG can be useful as an antioxidant agent.
研究领域:
有机化学
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药物化学
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天然产物化学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 8
·1733
·
IF 4.2
Shuo Zhang,Ze Liu,Xinyu Xu,Ruihua Zhao,Shujiang Zhang,Rong Luo
In traditional Chinese medicine, Aurantii Fructus Immatures (AFIs) have been utilized for more than 2000 years. The proportions of different fruit parts are crucial for evaluating AFI quality in China. However, the basis for this statement’s substance is unclear. Differences in quality are intimately correlated with a plant’s metabolite composition. On the basis of a widely targeted metabolome, this study intended to investigate the metabolite composition and evaluate the antioxidant capacity of the peel and pulp of an AFI. Metabolites were identified and quantified by UHPLC-QqQ-MS. To assess their antioxidant ability, DPPH and ABTS assays were carried out. There were 1327 chemical compounds identified by UHPLC-QqQ-MS. After screening the differential metabolites using a multivariate statistical analysis, it was found that there were 695 significant differences in the metabolites between the peel and the pulp. Among them, it was discovered that the content of active ingredients in the peel group was higher than that in the pulp group. Furthermore, the aqueous extracts from the peel showed stronger antioxidant capacities than those from the pulp. The metabolites and antioxidant capacities were significantly different between the peel and the pulp. This study of different fruit parts might provide a guide for AFI quality assessments.
研究领域:
药理学
·
中药学
·
代谢组学
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植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Aiyun Hu,Yijia Zhao,Lun Jiang,Zhiren Gan,Ruoxuan Wang,Cheng Lu,Jian Ding
This study aimed to optimize the extraction process of polysaccharides from seed pumpkins. Following initial single-factor experiments, the extraction process using ultrasonic-assisted enzymatic method was optimized through orthogonal experiments. Further optimization of extraction conditions was conducted using response surface methodology with Box-Behnken design, ultimately identifying the optimal extraction parameters. Additionally, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, monosaccharide component analysis, polysaccharide molecular weight measurement, and NMR analysis were performed. Results indicated that ultrasonic-assisted enzymatic extraction significantly enhanced the yield of polysaccharide, reaching up to 43.65 %. The findings confirm that the combined use of enzymatic hydrolysis and ultrasonication is an effective strategy for polysaccharide extraction.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2014
·
IF 2.468
Ru Song,Rong-bian Wei,Guang-qiang Ruan,Hong-yu Luo
The objective of this study was to identify antioxidative peptides from peptic hydrolysates of half-fin anchovy (HAHp). Furthermore, the possible synergistic effects of identified peptides were evaluated. HAHp were separated using CM FF ion exchange and Sephadex G 25 chromatography. HAHp1-2, one peptide fraction with the highest 1, 1-diphenyl-2-pycrylhydrazyl (DPPH) scavenging activity, was subjected to RP-HPLC coupled with ESI under positive ionization mode. Six peptides, AGDDARPA (771.35 Da), ESAGLHE (741.33 Da), NKVKGELD (901.45 Da), TLWSGAGQTVNT (1233.63 Da), EMSAGLHE (872.37 Da) and WRKKDPLND (1171.60 Da), were identified by ESI-MS/MS analysis. Peptides AGDDARPA (HAHp1-2Ⅰ), ESAGLHE (HAHp1-2Ⅱ), NKVKGELD (HAHp1-2Ⅲ), EMSAGLHE (HAHp1-2Ⅳ) and WRKKDPLND (HAHp1-2Ⅴ) were synthesized to determine in vitro antioxidative activity. Results showed that HAHp1-2Ⅳ and HAHp1-2Ⅴ displayed higher DPPH radical scavenging activities than other three peptides and l -carnosine. Peptide HAHp1-2Ⅲ demonstrated similar reducing power ability as l -carnosine. All synthetic peptides had moderate or negligible metal chelating activities. Additionally, the enhanced DPPH radical scavenging activities or reducing power abilities were observed for the mixture of HAHp1-2Ⅲ, HAHp1-2Ⅳ or HAHp1-2Ⅴ, indicating the possible synergistic effects. These results suggested that the antioxidative peptides derived from HAHp could be used as potential antioxidants in functional foods and nutraceuticals.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 9
·2865
·
IF 4.927
Xiuqiong Huang,Wei Gao,Xuan Yun,Zhixing Qing,Jianguo Zeng
In recent years, synthetic antioxidants that are widely used in foods have been shown to cause detrimental health effects, and there has been growing interest in antioxidants realised from natural plant extracts. In this study, we investigate the potential effects of natural antioxidant components extracted from the forage plant marigold on the oxidative stability of soybean oil. First, HPLC-Q-TOF-MS/MS was used with 1,1-diphenyl-2-picrylhydrazyl (DPPH) to screen and identify potential antioxidant components in marigold. Four main antioxidant components were identified, including quercetagetin-7-O-glucoside (1), quercetagetin (2), quercetin (3) and patuletin (4). Among them, quercetagetin (QG) exhibited the highest content and the strongest DPPH radical scavenging activity and effectively inhibited the production of oxidation products in soybean oil during accelerated oxidation, as indicated by reductions in the peroxide value (PV) and acid value (AV). Then, the fatty acids and volatile compounds of soybean oil were determined with gas chromatography–mass spectrometry (GC-MS) and headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS). A total of 108 volatile components, including 16 alcohols, 23 aldehydes, 25 ketones, 4 acids, 15 esters, 18 hydrocarbons, and 7 other compounds, were identified. QG significantly reduced the content and number of aldehydes and ketones, whereas the formation of acids and hydrocarbons was completely prevented. In addition, the fatty acid analysis demonstrated that QG significantly inhibited oxidation of unsaturated fatty acids. Consequently, QG was identified as a potential, new natural antioxidant that is believed to be safe, effective and economical, and it may have potential for use in plant extracts feed additives.Keywords:Tagetes erectaL.;quercetagetin;DPPH-HPLC-Q-TOF/MS;HS-SPME-GC-MS;oxidative stability
研究领域:
植物学
·
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）