ANALYTICAL LETTERS
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2023
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IF 2
Xiaoyu Zhang,Peiran Meng,Wei Wang,Xiwen Li,Zuan Yang,Shaokai Du,Yue Sun
Simultaneous determination of erythromycin (Ery) and hemoglobin (Hb) is of great significance for clinical diagnosis of many diseases. ZnO nanorods modified with Au nanoparticles were fabricated as an interface on the flexible carbon cloth (CC) and utilized to simultaneously determine Ery and Hb by a simple electrochemical method. The results demonstrated that Au/ZnO/CC displayed excellent analytical performance for Ery with a low detection limit of 3.38 × 10−7 mg/L and a linear range from 1 × 10−6 to 1 × 10−1 mg/L. At the same time, the sensor also showed a sensitive response to Hb with a low detection limit of 2.07 × 10−7 mg/L and a linear range from 1 × 10−6 to 1 × 10−1 mg/L. This work provides a viable approach for designing a flexible electrode and sensitive simultaneous determination of Ery and Hb.
研究领域:
分析化学
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纳米技术
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电化学
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生物传感器
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高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2021
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IF 1.134
Yan-Bing TAO,Jing HUANG,Yu-Nong ZHAO,Yan CHEN,Qing HUANG,Bo-Xiang SONG,Jian-Jun CHEN,Huan LIU
The electrochemical sensor fused with the principle of immunoassay can recognize the specific binding reaction of antigen and antibody, and transduce it directly into an electrical signal, realizing the rapid detection of relevant disease markers in the liquid sample. In this study, an electrochemical sensor for detecting eosinophil cationic protein (ECP), a marker of allergic rhinitis, was constructed based on a chemically modified electrode , and the surface of the carbon electrode was modified with semiconductor colloidal quantum dots (CQDs). Owing to the remarkable surface effect, size effect and unique quantum effect of CQDs, which can improve the enrichment of biomolecules and signal transduction on the surface of the carbon electrode, the lead sulfide (PbS) CQDs have good adhesion to the carbon electrode. On the basis of the ECP antibody coating and sealing treatment on the surface of the CQDs modified working electrode , the differential pulse voltammetry (DPV) was combined to significantly reduce the background current for electrochemical performance test, and then the quantitative detection of ECP antigen of different concentrations can be achieved. The detection time of the electrochemical sensor for ECP antigen was about 30 seconds, and the detection limit was 0.508 pg mL −1 . The electrochemical sensor has the characteristics of high sensitivity, convenience and non-invasiveness, showing the potential for early screening of diseases such as allergic rhinitis.
研究领域:
分析化学
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生物化学
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生物医学工程
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电化学
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高影响因子
MICROCHIMICA ACTA
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2018
·Vol. 185
·期 6
·1-8
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IF 5.705
Li Shuhuai,Li Jianping,Luo Jinhui,Xu Zhi,Ma Xionghui
An electrochemical microfluidic chip is described for the determination of the insecticide carbofuran. It is making use of a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. The analyte (carbofuran) is transported to the MIP and captured at the identification site in the channel. Then, carbofuran is eluted with carbinol-acetic acid and transported to the DNA aptamer on the testing position of the chip. It is captured again, this time by the aptamer, and detected by differential pulse voltammetry (DPV). The dual recognition (by aptamer and MIP) results in outstanding selectivity. Additionally, graphene oxide-supported gold nanoparticles (GO-AuNPs) were used to improve the sensitivity of electrochemical detector. DPV response is linear in the 0.2 to 50 nM carbofuran concentration range at a potential of −1.2 V, with a 67 pM detection limit. The method has attractive features such as its potential for high throughput, high degree of automation, and high integration. Conceivably, the method may be extended to other analytes for which appropriate MIPs and aptamers are available.
Schematic of an electrochemical microfluidic chip for carbofuran detection based on a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. In the chip, targets were recognized by MIP and aptamer, respectively. It shows promising potential for the design of electrochemical devices with high throughput, high automation, and high integration.
研究领域:
分析化学
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电化学
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环境科学
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微流控技术
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2020
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IF 3.594
Zhimei Wang,Di Zhang,Xilin Xiao,Changlin Su,Zhiyang Li,Jinhua Xue,Nan Hu,Pengcheng Peng,Lifu Liao,Hongqing Wang
Uranium is a kind of natural radionuclide, when the human body is exposed to higher uranium radioactive conditions, it will cause adverse effects on their health. Therefore, the analysis and detection of uranyl ions in the environment is a vital prerequisite for preventing uranium pollution. A bipolar bidentate ligand isophthalaldehyde-tetrapyrrole (IPTP) was first synthesized and a study was characterized by its structure and spectral properties. In the present work, an innovative electrochemical sensor by using IPTP as a ligand for uranyl (VI) ion (UO 2 2+ ), α-methacrylic acid as functional monomer and uranyl (VI) ion as a template, a relatively stable structure was constructed by stirring at 35 °C. Then, the polymer sol-gel was acquired by combining sol-gel processing under alkaline conditions, which was added dropwise to the surface of a carbon paste electrode (CPE) which had been modified with graphene (GR). The introduction of graphene allowed the reduction peak current of uranyl ions to be increased significantly. Under the optimum experimental conditions (pH=5 and the adsorption time=20 min), the ion-imprinted sensor was designed by adopting differential pulse voltammetry (DPV) for the sensing of uranyl (VI) ions. The detection technique successfully achieved the determination of uranyl ion in the concentration range 1.0 × 10 −10 mol L − 1 to 1.0 × 10 −7 mol L − 1 , in practical soil samples with excellent repeatability and recovery (97.3%-102.9%).
研究领域:
分析化学
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环境科学
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材料科学
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电化学
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DOI
高影响因子
FOOD CHEMISTRY
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2026
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IF 10.4
Xuran Zhang,Rongxian Ma,Zhili Li,Jing Cao,Haoqi Ma,Yeyu Wu,Xuecai Tan,Kejing Huang
The excessive use of sulfadiazine (SDZ) poses significant health risks due to toxic residues in food and ecosystems. This work introduces a novel molecularly imprinted polymer (MIP) sensor featuring a carbon dot-based covalent organic framework (CD-COF) core as a high-performance electrochemiluminescence (ECL) emitter. Upon SDZ rebinding to imprinted sites, the sensor leverages SDZ's inherent redox activity to enable complementary differential pulse voltammetry (DPV) detection alongside ECL quenching. This dual-signal output establishes an intrinsic cross-validation mechanism that inherently corrects environmental interferences. The sensor achieves ultrasensitive detection of SDZ, with impressively low detection limits of 0.28 nM (ECL) and 1.6 nM (DPV). Validation in food matrices demonstrates exceptional accuracy and reliability, yielding recoveries of 91–110%. This study not only provides a robust tool for trace SDZ monitoring but also establishes a new paradigm for CD-COF-powered MIP sensors integrating dual-mode signals for highly reliable contaminant analysis in real-world samples.
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PubMed
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Chunya Yu,Na Li,Ruiying Zhang,Dezhen Xie,Fei Li,Qiue Cao
Kanamycin (KAN) is a widely used aminoglycoside antibiotic that has attracted attention due to its presence in food and the environment. In order to obtain a simple, rapid, and sensitive method for kanamycin, an electrochemical sensor was fabricated by successively modifying a glassy carbon electrode (GCE) with reduced graphene oxide (rGO), poly(2-aminopyridine) (pAP) film, and a KAN molecularly imprinted membrane (KAN-MIM) that was prepared by electroreduction of graphene oxide and the electropolymerization of 2-aminopyridine and dopamine. The morphology and electrochemical parameters were characterized for the sensor designated by KAN-MIM/pAP/rGO/GCE. As a result of the amplification of the modified electrode, excellent analytical performance was obtained on the bases of sensitivity, selectivity, repeatability, and stability. The linear range and limit of detection for kanamycin were from 0.02 to 250.0 μmol·L−1 and 7.0 nmol·L−1, respectively. The sensor was employed to determine KAN in pork and milk with recoveries from 89.2% to 109.0% and a relative standard deviation less than 6.0%.
研究领域:
分析化学
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食品科学
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环境科学
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DOI
高影响因子
ANALYTICAL LETTERS
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2023
·
IF 2
Wenyan Tao,Mingguo Ye,Ying Liu,Zhenjia Lin,Chenchen Wang,Yujun Huang,Dawei Pan
An electrochemical sensor based upon a poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT-PSS) co-polymer film with gold nanoparticles (AuNPs) modified microelectrode is reported for the determination of Fe(III). Scanning electron microscopy images showed that the AuNPs were immobilized on gold (Au) microelectrode by formation of a nanofilm with PEDOT-PSS. The individual particles and their cluster-like aggregates provide a larger active electrochemical area and higher conductivity, which facilitates the determination of Fe(III). Electrochemical impedance spectroscopy (EIS) shows the AuNPs/PEDOT-PSS/Au microelectrode possesses high conductivity. A low detection limit of 0.01 µM Fe(III) was obtained using the modified microelectrode by differential pulse voltammetry (DPV) due to its large specific surface area, high conductivity, and the presence of more active sites. The optimized pH of the supporting acetate buffer was 2.0. The AuNPs/PEDOT-PSS/Au provided a linear range from 0.0119 μM to 7.14 μM. The modified electrode was successfully employed for the the determination of total dissolved iron in river water from Three Gorges with satisfactory results.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2023
·
IF 2
Qian Gao,Yang Li,Xinyi Li,Huiqing Wang,Lin Tian,Hao Gong,Cuiping Ma,Chao Shi
A highly ultrafast and sensitive electrochemical sensor based on electrical potential-assisted hybridization was developed for the determination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The electrode was modified with multi-walled carbon nanotubes (MWCNTs) and Au nanoparticles (AuNPs) for excellent electrochemical performance. The constant electric field was applied to rapidly and efficiently enrich the capture probes to the electrode surface in 60 s. Meanwhile, pyrene as the backfill reagent resulted in a significant reduction of nonspecific adsorption. The electrochemical properties of the modified electrode were investigated via electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The analytical response was measured by differential pulse voltammetry (DPV). The sensor achieved the ultrafast hybridization of nucleic acids at the electrode interface within 90 s, substantially reducing the reaction time from hours to minutes. Under optimal conditions, the sensor exhibited a superior detection limit of 60 fM and showed satisfactory specificity toward single-base mismatch. Additionally, the proposed sensor realized sensitive determination of SARS-CoV-2 in throat swabs. The enzyme-free and non-amplification sensing strategy showed promising potential for application in point-of-care testing (POCT).
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高影响因子
ANALYTICAL LETTERS
·
2023
·
IF 2
Jian Huang,Guolin Yuan,Dongliang Cheng,Fei Xie,Xianru Xia,Xiandong Li,He An,Chunyan Peng
Atherosclerotic coronary heart disease (CHD) is ischemic and a worldwide health issue. The soluble suppression of tumorigenicity 2 (sST2) is a novel biomarker for atherosclerotic coronary heart disease. This work develops an immunosensor with a nanocomposite consisting of polyaniline (PANI) and MXene-Nafion for the specific and sensitive determination of sST2. PANI was first electropolymerized on a glassy carbon electrode (GCE) to enhance electron transfer. Subsequently, MXene-Nafion dispersion was drop cast on the PANI/GCE substrate to further increase the electrical conductivity and immobilize anti-sST2 which captures sST2 for detection. The current response as a function of sST2 concentration was measured by differential pulse voltammetry (DPV). Under the optimal conditions, this immunosensor demonstrates a linear range from 1 pg mL−1 to 1 μg mL−1 and a detection limit of 0.149 pg mL−1. The device offers high sensitivity, excellent specificity, satisfactory reproducibility, good stability, and has significant potential for detecting clinical biomarkers. In addition, the immunosensor is able differentiate between serum of healthy people and CHD patients.
研究领域:
医学诊断
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生物传感技术
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DOI
高影响因子
ANALYTICAL LETTERS
·
2023
·
IF 2
Yuanjie Teng,Xin Li,Weihao Huang
Reliable quantitative analysis using electrochemical sensors remains a challenge due to the differences in the effective area after surface modification of the electrodes, resulting in poor precision of the signals between electrodes. Herein, a novel quantitative approach called standard curved surface for the electrochemical detection is reported which does not require identical electrodes, high modification, or the use of internal standards that may cause calibration interferences. The technique is based upon the principle that the current is linearly correlated with the electroactive area of the electrodes. By introducing the electroactive area as a new variable into the standard curve, a three-dimensional standard curved surface was obtained to correct for differences among electrodes, thereby improving the accuracy of electrochemical detection. Furthermore, it was applied to the determination of tert-butylhydroquinone (TBHQ) in edible oil. Compared with the traditional standard curve, the reported method reduced the relative standard deviation values from 13.22% to 4.01%. The current response was linear with the concentration of TBHQ between 5 × 10−6 and 1.25 × 10−3 mol/L. The limit of detection was 1.07 × 10−6 mol/L and the spike recovery was from 90.44% to 112.09%. Therefore, a universal calibration method has been reported with expected additional applications for electrochemical sensors.
研究领域:
分析化学
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食品科学
·
电化学传感器
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Hui Lv,Yueyun Li,Xiaobo Zhang,Zengqiang Gao,Jinhui Feng,Ping Wang,Yunhui Dong
In this work, bimetallic core-shell rhodium@palladium nanodendrites (Rh@Pd NDs) loaded on sulfo group functionalized multi-walled carbon nanotubes (MWCNTs-SO 3 H) were combined to form Rh@Pd NDs/MWCNTs-SO 3 H nanocomposites. And the composites were used to construct a simple and label-free electrochemical immunosensor for carcino embryonic antigen (CEA) detection using differential pulse voltammetry (DPV). Rh@Pd NDs with dendritic nanostructure not only provide abundant catalytically active sites, but also increase the loading of antibody, which could improve the analytical performance and result in high sensitivity. In addition, the MWCNTs-SO 3 H could further enhance electrochemical properties due to the excellent conductivity, good solubility and high surface area. Taking advantages of both Rh@Pd NDs and MWCNTs-SO 3 H, the proposed immunosensor showed a broad linear range from 25 fg mL −1 to 100 ng mL −1 for CEA detection and a low detection limit of 8.3 fg mL −1 (signal-to-noise ratio of 3) under optimal experimental conditions. Moreover, the expected immunosensor exhibited good reproducibility and high sensitivity, which could achieve excellent analysis of CEA in human serum with satisfactory results. Therefore, the Rh@Pd NDs/MWCNTs-SO 3 H nanocomposites may be considered as a sensing platform for fabrication of simple, ultrasensitive and label-free electrochemical immunosensor.
研究领域:
纳米技术
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电化学
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生物传感器
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分析化学
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癌症诊断
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Wei Chen,Chao Yan,Lin Cheng,Li Yao,Feng Xue,Jianguo Xu
In this paper, a novel ultrasensitive signal-on electrochemical aptasensor has been proposed for Ochratoxin A (OTA) assay based on DNA controlled layer-by-layer assembly of dual gold nanoparticle (AuNP) conjugates. To construct the aptasensor, the 1 st AuNP conjugate was prepared by simultaneous immobilization of the capture probe 2 (CP2) and bridge probe (BP) onto the AuNPs. Then, OTA aptamer was loaded onto 1 st AuNPs by hybridization with CP2. The 1 st AuNP conjugate can be further immobilized onto the electrode by hybridization between BP and capture probe 1 (CP1), which was pre-immobilized on Au electrode. The 2 nd AuNP conjugate was prepared by immobilization of ferrocene (Fc) tagged SH-signal probe (SSP). Due to the recognition between aptamer on 1 st AuNP conjugate and OTA, CP2 was reformed in the ssDNA state, which can be utilized as the anchor for immobilization of 2 nd AuNP conjugate for electrochemical signal reporting. Because of the high surface-to-volume ratio and good conductivity of AuNPs, this dual AuNPs assembled nanoarchitecture finally lead to greatly improved abilities to load a large number of Fc molecules and significantly amply the electrochemical response even at a low target concentration. Both qualitative and quantitative analysis of OTA were thus realized by differential pulse voltammetry (DPV) signals, resulting in an excellent detection limit of 0.001 ppb and a wide dynamic range from 0.001 to 500 ppb over 6 orders of magnitude. Moreover, the real sample analysis towards OTA spiked wine samples was favorable, implying a great potential for practical applications. We envision that this unique dual AuNP conjugate assembly strategy would pave a new avenue for the development of versatile signal amplified electrochemical aptasensors.
研究领域:
分析化学
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纳米技术
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食品安全
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毒理学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）