INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2018
·Vol. 19
·期 3
·728
·
IF 3.687
Chunyan Jiao,Cheng Song,Siyan Zheng,Yingpeng Zhu,Qing Jin,Yongping Cai,Yi Lin
Alkaloids are the main active ingredients in the medicinal plantDendrobium officinale. Based on the published genomic and transcriptomic data, a proposed terpenoid indole alkaloid (TIA) biosynthesis pathway may be present inD. officinale. In this study, protocorm-like bodies (PLBs) with a high-yielding production of alkaloids were obtained by the optimization of tryptophan, secologanin and methyl jasmonate (MeJA) treatment. The results showed that the total alkaloid content was 2.05 times greater than that of the control group when the PLBs were fed with 9 µM tryptophan, 6 µM secologanin and 100 µM MeJA after 36 days. HPLC analysis showed that strictosidine synthase (STR) activity also increased in the treated plants. A total of 78 metabolites were identified using gas chromatography-mass spectrometry (GC-MS) in combination with liquid chromatography-mass spectrometry (LC-MS) methods; 29 differential metabolites were identified according to the multivariate statistical analysis. Among them, carapanaubine, a kind of TIA, exhibiteddramaticallyincreased levels. In addition, a possible underlying process of the metabolic flux from related metabolism to the TIA biosynthetic pathway was enhanced. These results provide a comprehensive view of the metabolic changes related to alkaloid biosynthesis, especially TIA biosynthesis, in response to tryptophan, secologanin and MeJA treatment.Keywords:Dendrobium officinale;metabolomics;alkaloids;tryptophan;secologanin;methyl jasmonate
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高影响因子
Heliyon
·
2019
·
IF 0
Yingpeng Zhu,Jiangliu Yu,Chunyan Jiao,Jinfeng Tong,Lei Zhang,Yan Chang,Weina Sun,Qing Jin,Yongping Cai
Ultrasonic-assisted extraction of quercetin from Dendrobium officinale was optimized by response surface methodology (RSM) using high-performance liquid chromatography as a separative method. Based on single-factor experiments and two-level factorial analysis, the ethanol concentration, solid-to-liquid ratio and ultrasonic power were selected as significant response factors. The amount of quercetin that we extracted from Dendrobium officinale was 2.506–2.594 μg/g under the extraction conditions, which showed that optimization could improve the extration rate of quercetin from Dendrobium officinale . Quercetin was extracted and detected within 12 consecutive months after the germination of Dendrobium officinale by optimizing the extraction process to analyze the accumulation of quercetin. The UV-B exposure experiments showed that the Dendrobium officinale leaves have different responses to low- and high-dose UV light. The results showed that the quercetin content in Dendrobium officinale could be changed by UV-B radiation, and the response of distinct tissue parts to varying intensities of UV-B radiation was different.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2020
·Vol. 21
·期 17
·6250
·
IF 4.556
Can Si,Jaime A. Teixeira da Silva,Chunmei He,Zhenming Yu,Conghui Zhao,Haobin Wang,Mingze Zhang,Jun Duan
The acetylation or deacetylation of polysaccharides can influence their physical properties and biological activities. One main constituent of the edible medicinal orchid,Dendrobium officinale, is water-soluble polysaccharides (WSPs) with substitutedO-acetyl groups. BothO-acetyl groups and WSPs show a similar trend in different organs, but the genes coding for enzymes that transfer acetyl groups to WSPs have not been identified. In this study, we report that REDUCED WALL ACETYLATION (RWA) proteins may act as acetyltransferases. ThreeDoRWAgenes were identified, cloned, and sequenced. They were sensitive to abscisic acid (ABA), but there were no differences in germination rate and root length between wild type and35S::DoRWA3transgenic lines under ABA stress. Three DoRWA proteins were localized in the endoplasmic reticulum.DoRWA3had relatively stronger transcript levels in organs where acetyl groups accumulated thanDoRWA1andDoRWA2, was co-expressed with polysaccharides synthetic genes, so it was considered as a candidate acetyltransferase gene. The level of acetylation of polysaccharides increased significantly in the seeds, leaves and stems of three35S::DoRWA3transgenic lines compared to wild type plants. These results indicate that DoRWA3 can transfer acetyl groups to polysaccharides and is a candidate protein to improve the biological activity of other edible and medicinal plants.Keywords:acetyl groups;Dendrobium officinale;REDUCED WALL ACETYLATION;endoplasmic reticulum
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yuan Zhang,Lu Yu,Wuxia Ge,Wentao Bi,David Da Yong Chen
A carbon-rich material (DESysChar) was prepared from polysaccharide within a deep eutectic system (DESys) containing oxalic acid, and systematically characterized using various analytical techniques. The investigation of reaction mechanism revealed concurrent dehydration and etherification processes. This study commenced with the extraction of plant polysaccharide using the DESys-based mechanochemical extraction method from Dendrobium officinale . Subsequently, the DESys method was used to carbonize the extracted Dendrobium officinale polysaccharide and produce DESysChar. DESysChar was then used for the adsorption and determination of pollutants in water. This study represents a significant advancement in eco-friendly material synthesis, enabling the low-temperature (120 °C) carbonization of plant-derived polysaccharides, thereby reducing energy consumption and environmental impact. The effective adsorption of methylene blue by DESysChar underscores its potential in environmental remediation. This study presents a more responsible and efficient approach to polysaccharide extraction and carbonization, addressing environmental concerns. Embracing the 4S workflow (involving Sustainable raw materials converted into Sustainable degradable products, by using Sustainable technology throughout the process to create a Sustainable environment) promotes sustainability in material development, laying the foundation for future eco-friendly practices in various industries. In summary, this study propels sustainable polysaccharide development for widespread use.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Cunchao Zhao,Weiqian Wang,Fanrui Li,Jun Sheng,Rui He,Lijun You,Yang Tian
This study compared six extraction techniques, hot water, acid-assised, ultrasound-assisted, microwave-assisted, UV/H₂O₂-assisted, and enzyme-assisted on the structural, antioxidant, and emulsifying properties of Dendrobium officinale polysaccharides (DOPs). Molecular weights ranged from 1155.239 kDa (DOPs-hwe) to 3.139 kDa (DOPs-ee). DOPs-hwe showed the highest uronic acid content (4.52 %) and excellent emulsifying ability (89.2 % activity, 81.5 % stability). In contrast, DOPs-ae and DOPs-ee exhibited stronger antioxidant activity, with DPPH scavenging rates of 52.36 % and 58.05 % (15 mg/mL), linked to lower molecular weights and higher carboxyl group contents (0.58–0.66 mmol/g). FTIR and Congo red analyses confirmed differences in triple-helix conformations. SEM imaging revealed morphological diversity, from dense (DOPs-hwe) to porous (DOPs-ee). Monosaccharide composition varied across extraction methods. Emulsifying performance was mainly influenced by hydrophobic and steric interactions. These findings provide a useful reference for selecting suitable extraction approaches to tailor DOP functionality for specific applications in food, cosmetic, and pharmaceutical industries.
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IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
·
2026
·
IF 2.2
He Xiaotong,Dou Qiumei,Huang Xi,Zhang Xinyu,Zheng Ziqi,Wang Yingzi,Yang Quan,Gao Hongyang
Dendrobium officinale regenerated plants were divided into a normal control group (Normal), a hyperhydricity group (HH), and a silicon-treated hyperhydricity group (HH and Si); and the study investigated how silicon mitigates hyperhydricity by regulating water metabolism, photosynthetic pigments, antioxidant system, ethylene-polyamine metabolism, stomatal morphology, and molecular mechanisms. The results revealed significant differences between the HH group and the Normal group. The HH group exhibited a substantial increase in free water content, as well as reduced photosynthetic pigment content, diminished antioxidant enzyme activity, elevated ethylene levels, lower polyamine concentrations, and impaired stomatal regulation. In contrast, the HH and Si group exhibited recovered free water content to Normal group levels, markedly increased photosynthetic pigments, enhanced antioxidant capacity, reduced ethylene content, elevated polyamine levels, and restored stomatal function. Furthermore, silicon supplementation suppressed the expression of ethylene synthesis genes and increased that of polyamine synthesis genes. These findings indicated that silicon alleviates hyperhydricity in D. officinale regenerated plants through multifaceted mechanisms. Specifically, silicon mitigated the adverse effects of hyperhydricity by: (1) restoring cellular water balance and minimizing structural damage from free water accumulation; (2) recovering photosynthetic pigment content and boosting antioxidant enzyme activity; and (3) rebalancing ethylene-polyamine antagonism to improve stomatal function. This study provided a theoretical foundation and technical framework for managing hyperhydricity in D. officinale regenerated plants.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Ruichen Zhao,Like Wang,Jianhua Zhu,Shouwei Yin
Environmental oxidative stress critically drives skin aging and damage. Although the natural flavonoid naringin (NAR) offers significant antioxidant and anti-aging potential, its cosmeceutical application is limited by poor solubility and stability. To address this, we engineered Dendrobium officinale polysaccharide (DOP)-coated phosphorylated zein (P-Zein) nanoparticles (DOP-PZ-NAR) for NAR encapsulation. Optimized nanoparticles exhibited uniform spherical morphology (92.9–157.0 nm) with exceptional drug-loading performance (encapsulation efficiency: 87.50 %). DOP-PZ encapsulation significantly enhanced NAR photostability, increasing retention from 62.40 % to 84.30 % after 12 h of UV exposure, and significantly improved in vitro antioxidant efficacy (> 86 % ABTS, > 80 % DPPH at 500 μg/mL NAR equivalent). Furthermore, DOP-PZ encapsulation significantly enhanced transdermal delivery, achieving a 3.42-fold increase in cumulative NAR permeation versus free NAR while enabling deeper skin penetration. Notably, in vivo zebrafish assays confirmed DOP-PZ-NAR NPs were non-toxic and elicited a potent melanin-inhibitory effect (67.23 % reduction at 30 μg/mL NAR equivalent). This study demonstrates DOP-PZ-NAR NPs as a multifunctional platform for stabilizing hydrophobic antioxidants and enhancing their cutaneous bioavailability, while offering novel insights for developing next-generation cosmeceutical formulations.
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Food & Function
·
2022
·Vol. 13
·期 13
·7274-7286
·
IF 6.317
Zhijing Wu,Yu Zhang,Gang Nie,Junxi Liu,Hao Mei,Zihao He,Pengfei Dou,Kaiping Wang
Recently, Dendrobium officinale polysaccharide (DOP), a typical acetylated glucomannan, has been widely applied in functional foods owing to its excellent bioactivity. However, the insufficiency of studies on in vivo process severely limits the further utilization of DOP. The aim of this study was to systematically investigate the gastrointestinal digestive behaviour of DOP after oral administration by labelling it with two fluorescein aminopyrene-1,3,6-trisulfonic acids, trisodium salt (APTS) and cyanine 7.5 (Cy7.5). Combining the results of NIR imaging and HPGPC, we found that DOP was poorly absorbed directly in the prototype form; instead, DOP moved with the intestinal contents to the distal part of the intestine, where Bacteroides aggregated for a prolonged time and was metabolized to oligosaccharide-like substances. In contrast, the digestive degradation of DOP in pseudo-sterile mice with a targeted clearance of Bacteroides significantly weakened, which provided the basis and direction for the subsequent search for more specific metabolic pathways of DOP in vivo.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·
IF 5.8
Mu Yi,Che Bangwei,Tang Kaifa,Zhang Wenjun,Xu Shenghan,Li Wei,He Jun,Liu Miao,Chen Peng,Zhong Siwen,Li Guangyu
Polysaccharides from Dendrobium officinale polysaccharides ( DOP s) are the main bioactive components of Dendrobium officinale , which have the functions of antioxidation and immune regulation. However, it is not clear whether DOPs have any effect on the prevention of reproductive disorders induced by oxidative stress. The purpose of this study was to explore the protective effect of DOPs on reproductive oxidative stress injury in male mice and its possible mechanism. In this study, the mouse model of reproductive injury was established by intraperitoneal injection of cyclophosphamide (CTX). The reproductive function was evaluated by relative testicular mass, sperm parameters, and sex hormone levels. The oxidative stress level of male mice with reproductive injury treated with DOPs was analyzed by the levels of 8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA), and nitric oxide (NO) in sperm. The expression of follicle-stimulating hormone receptor ( FSHR ) mRNA, androgen-binding ( ABP ) mRNA, and c-kit mRNA was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) to explore its mechanism. After CTX administration, the sperm density, sperm motility, normal sperm morphology, and sex hormone levels in mice were significantly lower than those in the control group ( P < 0.05). At the same time, the expression of p53 protein was upregulated, and the expression of Bcl-2 protein was downregulated ( P < 0.05). In addition, the expression of FSHR and ABP mRNA on Sertoli cells was also significantly inhibited ( P < 0.05). DOPs can effectively reduce the oxidative stress injury of testicular tissue. After DOP treatment, the sperm quality and sex-related hormone levels of mice were significantly improved and positively correlated with the dose of DOPs ( P < 0.05). Administration of DOPs can reduce the damage caused by oxidative stress by reducing the level of oxidative stress, improving the hormone environment in testes, and regulating the expression of specific genes in Sertoli cells and spermatogenic cells.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Yan Fu,Quansheng Wang,Liang Zhang,Shuping Ling,Huiyan Jia,Yinliang Wu
The residual behaviors and dietary risk probability of 12 pesticides in Dendrobium officinale Kimura et Migo cultivated at two representative locations under green house conditions were investigated using liquid chromatography-tandem mass spectrometry. Field trials showed that the half-lives of 12 pesticides ranged from 0.9 to 14.4 days in fresh D. officinale stems. Based on maximum residue levels (MRLs), the ultimate residues of imidacloprid, dimethomorph, metalaxyl, tebuconazole, and cyazofamid at a pre-harvest interval (PHI) of 28 days were within acceptable limits. For abamectin, indoxacarb, and difenoconazole, 35-day PHIs were needed. The PHIs of trifloxystrobin and fluopyram were 42 days, the time required for their residues to be reduced to an MRL of 4 mg/kg. The chronic and acute risk quotients of target pesticides at PHIs of 28–42 days were below 5.929% and 0.532%, respectively, showing that the evaluated D. officinale exhibited an acceptably low dietary risk to the general population.
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Journal of Food Measurement and Characterization
·
2024
·
IF 2.9
Feng Simin,Liang Shuqi,Sui Minghui,Yu Jiahao,Lin Yang,Gu Hong,Sun Peilong,Shao Ping
Flavonoids play a major role in promoting human health, yet their instability in solution greatly limits its industrial utilization. This study aimed to introduce soybean peptides (SP) to improve the stability and solubility of Dendrobium officinale flavonoids (DOFs), and to investigate the effects of their interaction, sterilization and storage. The results revealed that DOFs bind to SP through hydrogen bonding and steric effects, leading to changes in the structure, energy, and activity of DOFs, which resulted in enhanced stability and antioxidant capacity. The sedimentation rate and flavonoid content were measured to be 24.64 ± 0.55%, 3.83 ± 0.14 mg/mL, respectively, at the optimum mass ratio of DOFs and SP of 2:5. After thermal treatment, the flavonoid content and antioxidant activity of the complexes both dramatically enhanced. The optimum sterilization condition was 75 °C for 20 min, while the optimum range of temperatures for storage was 4 ~ 25 °C. These findings provide valuable insights for the processing, preservation and application of flavonoids in the future.
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Food Bioscience
·
2025
·
IF 5.9
Bowen Sui,Yanling Sun,Jingzhi Wang,Yuan Cui,Haibo Xu,Daqing Zhao,Meichen Liu,Zhengqi Dong,Siming Wang,Shichao Liu
Dendrobium officinale Kimura et Migo has been used extensively in functional foods and garnered significant attention because of its therapeutic properties and nutritional value. Anthocyanins are prone to degradation in environmental and biological conditions, leading to color fading and loss of bioactivity. Forming complexes with endogenous polysaccharides can enhance their stability and protect the color of anthocyanin products. In this study, anthocyanins extracted from D. officinale (DOA) were complexed with D. officinale polysaccharide extract (DOP) to investigate the effects of complexation on the physicochemical properties of the anthocyanins. DOA–DOP complexes were characterized by scanning/transmission electron microscopies, differential scanning calorimetry, dynamic light scattering, and multiple spectroscopies. Stability assessments and simulated in vitro gastrointestinal digestion were performed. Bioactivity was evaluated using a human colon epithelial (Caco-2) cell monolayer model. Spectral analyses revealed hydrogen bonding as the primary interaction in the DOA–DOP complexes. Complexation significantly enhanced DOA retention under light exposure, heat treatment, pH variation, and exposure to metal ions. DOP improved the in vitro antioxidant capacity of DOA. Furthermore, the DOA–DOP complexes exhibited prolonged resistance to upper gastrointestinal digestion and restored lipopolysaccharide-induced epithelial hyperpermeability in the Caco-2 cell model. Collectively, DOA–DOP complexation enhanced D. officinale anthocyanin stability, bioactivity, and colonic bioavailability.
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