PEST MANAGEMENT SCIENCE
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2025
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IF 3.8
Jiayao Fan,Huimin Pan,Chenyang Yuan,Tianyuan Liu,Feng Shang,Yuxi Zhou,Yifan Gao,Long Yi,Jinjun Wang,Wei Dou
BACKGROUND Phenotypic plasticity is generally regarded as a crucial adaptation that enables organisms to survive in the face of environmental changes. Diaphorina citri , a vector of Candidatus Liberibacter asiaticus ( C Las) that causes citrus Huanglongbing, displays three distinct body color morphotypes: blue/green, gray/brown, and orange/yellow morphs. Here, dynamics monitoring of D. citri population in the field was established to identify the occurrence peak. The orange/yellow is a shared phenotype, and hence we focused on the two most prevalent color morphs blue/green and gray/brown. We evaluated the life-history characteristics and susceptibility to insecticides of the two color morphs. For the association between the body color polyphenism and C Las, we compared the C Las acquisition efficiency of field and laboratory populations, and investigated immunological competence of the two color morphs. RESULTS Diaphorina citri adults were present in all seasons in year one, with higher incidences recorded in July, August, and September. During these periods, the blue/green morph predominated in the field, while during the overwintering period, the proportion of gray/brown morph increased. Population parameters showed no significant differences between the two color morphs. Additionally, gray/brown morphs were more susceptible to insecticides and C Las acquisition. Furthermore, the two color morphs displayed different immune responses to pathogen infection. CONCLUSION The C Las infection rate was closely associated with color morphology in D. citri . Different field distribution and trait combinations of the two color morphs represent their responses to variable environments. This study not only broadens the understanding of color polyphenism, but it may also provide potential implication for controlling strategies in D. citri . © 2025 Society of Chemical Industry.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Wenjie Wang,Muhammad Adeel Ghafar,Lu Liuyang,Inzamam Ul Haq,Li Cui,Huizhu Yuan,Liande Wang
RNA pesticides have emerged as a promising alternative to conventional chemical pesticides due to their high specificity and minimal environmental impact. However, the instability of RNA molecules in the environment and the challenges associated with their effective delivery to target pests limit their broader application. This study addresses these challenges by developing a dual delivery system using chitosan (CS) and Metal–Organic Frameworks (MOFs) to enhance the delivery and efficacy of double-stranded RNA (dsRNA) and cycloxaprid against Diaphorina citri, a vector of citrus greening disease. The CS-MOF nanoparticles were synthesized and characterized using scanning electron microscopy (SEM) and dynamic light scattering (DLS). Insect bioassays demonstrated that the codelivery system significantly improved insecticidal activity, achieving over 80% mortality in D. citri within 2 days. The results indicate that the encapsulation of dsRNA within MOFs enhances its stability, while the controlled release properties of the nanoparticles improve the efficacy of cycloxaprid. This novel approach shows great potential in overcoming the limitations of RNA pesticides and offers a sustainable solution for pest management in agriculture. Future research should optimize the delivery system, conduct field trials, and explore its applicability to other agricultural pests.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
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IF 5.7
Luyang Liu,Xuanyue Yu,Yuting Huang,Cuiting Liu,Xinyi Xie,Zhongzhen Wu,Jintian Lin,Benshui Shu
Diaphorina citri is a serious citrus pest. Dinotefuran is highly insecticidal against D. citri. To analyze the sublethal effects of dinotefuran on D. citri adults, an indoor toxicity test was performed, which revealed that the lethal concentration 50 (LC50) values were 4.23 and 0.50 μg/mL for 24 and 48 h treatments, respectively. RNA-Seq led to the identification of 71 and 231 differentially expressed genes (DEGs) after dinotefuran treatments with LC20 and LC50 doses, respectively. Many of the DEGs are significantly enriched in the apoptosis pathway. Dinotefuran-induced apoptosis in the gut cells was confirmed through independent assays of 4′,6-diamidino-2-phenylindole (DAPI) and TdT-mediated dUTP nick end labeling (TUNEL) staining. Increased levels of reactive oxygen species (ROS) and a loss of mitochondrial membrane potential were observed. Four caspase genes were identified, and dinotefuran treatments resulted in increased mRNA levels of DcCasp1 and DcCasp3a. These findings shed light on the sublethal effects of dinotefuran on D. citri.
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高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2023
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IF 4.7
Cong Zhang,Bin Wan,Meng-Ru Jin,Jing Wang,Tian-Rong Xin,Zhi-Wen Zou,Bin Xia
The citrus industry has suffered severe losses as a result of Huanglongbing spread by Diaphorina citri . Controlling the population of D. citri is the key to preventing and controlling the spread of Huanglongbing. Ecdysteroids are key hormones that regulate insect development and reproduction. Therefore, the Halloween gene family involved in the ecdysone synthesis of D. citri is an ideal target for controlling the population growth of this insect. In this study, we successfully cloned four Halloween genes expressed during D. citri development. Silencing of one of the four genes resulted in a significant decrease in 20E titers in nymphs and significant decreases in the developmental, survival and emergence rates. Inhibiting Halloween gene expression in adults impeded the growth of the female ovary , diminished yolk formation , lowered vitellogenin transcription levels, and hence impaired female fecundity. This showed that Halloween genes were required for D. citri development and reproduction. DcCYP315A1 and DcCYP314A1 were highly expressed when D. citri was exposed to thiamethoxam and cypermethrin, and silencing these two genes made D. citri more sensitive to these two pesticides . Inhibition of DcCYP315A1 and DcCYP314A1 expression not only significantly delayed the development and reproduction of D. citri but also increased its susceptibility to pesticides. Therefore, these two genes are more suitable as potential target genes for controlling D. citri .
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高影响因子
危险品化学品经营许可证（带存储）