PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2026
·
IF 4.8
Jiawei Lei,Yushan Li,Mingxin Bai,Haoyue Du,Xueqin Shen,Hu Fu,Zhengli Yan,Yongfei Zhu
Xenobiotics such as pesticides can easily damage the reproductive organs during adolescence. Dinotefuran, the latest neonicotinoid insecticide, is considered to have low toxicity in vertebrates. However, research on its reproductive toxicity, especially damage to the female reproductive system in mammals is scarce. In this study, in vivo uterine toxicity was induced by oral administration of dinotefuran to mice at a dose ≥1/2 ADI (0.1 mg/kg/day), while in vitro toxicity was resulted from exposing cultured uteri to dinotefuran at levels equivalent to human blood levels (30–6000 ng/L). Hematoxylin-eosin staining revealed that dinotefuran caused uterine damage both in vivo and in vitro , while transmission electron microscopy detected mitophagy in the uterine cells. Furthermore, this study confirmed that dinotefuran triggered oxidative stress (decreased SOD and GSH, and elevated MDA), reduced mtDNA, and disrupted the BAX-to-BCL ratio in the uterus. Double immunofluorescence, qPCR, and western blot analyses found that dinotefuran induced excessive PRKN-independent mitophagy regulated by BNIP/NIX in the uterus, along with activation of NF-κB/NLRP3 signaling and the necroptosis pathway. It also revealed that dinotefuran increased uterine Caspase-1 /Cleaved CASPASE-1 levels by qPCR and western blot analysis. Collectively, these findings indicate that dinotefuran at concentrations ≥1/2 ADI value or equivalent to human blood levels induces mouse uterine toxicity in vivo and in vitro , respectively, and they all trigger oxidative stress, BNIP3/NIX-mediated mitophagy, NF-κB/NLRP3 signaling, and necroptosis in the uterus.
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高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Qisi Xu,Minmin Li,Ouli Xiao,Jieyin Chen,Xiaofeng Dai,Zhiqiang Kong,Jianxin Tan
Yellow rice wine (Huangjiu) is a traditional Chinese alcoholic beverage. However, there is a risk of pesticide residues in Huangjiu due to pesticide indiscriminate use. In this study, the residues of dinotefuran and its metabolites during Huangjiu fermentation and their effects on flavor substances were studied. The initial concentrations of dinotefuran ranged from 856.3 to 1874.9 μg/L, and its half-life was no more than 3.65 d. At 24 d of Huangjiu fermentation, the terminal residues of dinotefuran, 1-methyl-3-(tetrahydro-3-furylmethyl)urea (UF) and 1-methyl-3-(tetrahydro-3-furylmethyl)guanidine (DN) were 195.1–535.3 μg/L, 38.33–48.70 μg/L and 37.8–74.1 μg/L, respectively. Twenty potential degradation compounds were identified by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS), and their toxicity was evaluated. Finally, the effect of dinotefuran on physicochemical properties and total phenol content of Huangjiu were analyzed. The risk of rancidity was significantly increased and bitter amino acids were formed. These findings provide a guidance and the safe production of Huangjiu.
研究领域:
食品科学
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化学工程
·
毒理学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
·
IF 6.2
Lingyun Fan,Jia Wang,Chen Wang,Xujia Zhang,Qi Li,Hanxi Wang,Yi Liu,Yuan Hui Zhao,Shuying Zang
Neonicotinoids are widely used pesticides around the world, but the photolysis of neonicotinoids in cold agricultural region are still in blank. This paper aimed to study the influence of cold temperature over photolysis of neonicotinoids. To this end, the photolysis rates and photoproducts of dinotefuran and nitenpyram in water, ice and freeze-thawing condition were determined. Coupled with quantum chemistry calculation, the influence mechanisms of temperature and medium were investigated. The results showed the photolysis rates of neonicotinoids in water condition slightly declined with the lowered temperature due to the photolysis reactions were endothermic reactions. However, the photolysis rates increased by 89.8 %, 59.2 %, 49.4 % and 9.5 % for dinotefuran and nitenpyram in ice and thawing condition, respectively. This phenomenon was posed by the concentration-enhancing effect and change of photo-chemical properties of neonicotinoids in ice condition, which included lowered bond cleavage energy, lowered first excited singlet state energy and expanded light absorption range. The photolysis pathways of the two neonicotinoids did not change in different medium, but the concentration of carboxyl products was relatively higher than that of water condition due to the more amounts of reactive oxygen species in ice medium, which might increase the secondary pollution risk after ice-off in spring due to the higher ecotoxicity to nontarget organism of these photoproducts. The influence of cold temperature and medium change should be considered for the environmental fate and risk assessment of neonicotinoids in cold agricultural region.
研究领域:
环境科学
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农药化学
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DOI
PubMed
高影响因子
ELECTROANALYSIS
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2016
·Vol. 28
·期 7
·1495-1503
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IF 2.471
M. Zhang,X. C. Zhai,X. Yang,H. T. Zhao,A. J. Dong,H. Zhang,J. Wang,G. Y. Liu
A rapid method for sensitive voltammetric determination of dinotefuran residue was reported. The proposed method was based on the electrocatalytic reduction of dinotefuran on β-cyclodextrin-graphene composite modified glassy carbon electrode (β-CD-rGO/GCE), giving rise to a higher reduction signal to dinotefuran relative to the bare (GCE) and graphene modified electrode (rGO/GCE). Moreover, a further signal enhancement was observed when the modified electrode incubated in solution at low temperature (0 °C) for a short time. The reduction mechanism and binding affinity were also discussed. The external standard calibration curve was obtained from linear sweep voltammetry in the range of 0.5 to 16.0 μM with a detection limit of 0.10 μM. In addition to optimization of pretreatment, this electrochemical method has been applied to the dinotefuran residue determination in millet samples with the detection limit of 0.01 mg kg −1 and compared with an high performance liquid chromatography method. The proposed electrode and analysis methods were proven to be sensitive, accurate and rapid under the used conditions.
研究领域:
分析化学
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环境科学
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农业化学
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DOI
高影响因子
Journal of Environmental Sciences
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2026
·
IF 6.5
Zhaoxia Fan,Xinyue Liu,Jiawen Huang,Jianjian Wu,Fei Ge,Na Liu
Cd accumulation in rice exhibited enantioselectivity under Cd-DIN co-exposure. • S -DIN effectively reduced Cd bioavailability and root-to-grain translocation. • OsABCG14 mediated enantioselective Cd-DIN transport via differential binding. • OsABCG14 exhibited preferential binding to Cd- R -DIN over Cd- S -DIN. The interactions between heavy metals and chiral pesticides within agricultural ecosystems remain largely neglected, obscuring environmental behaviors. This study investigated the accumulation and translocation of cadmium (Cd) and chiral dinotefuran (DIN) in rice ( Oryza sativa L . ) under varying co-exposure conditions (hydroponics versus soil; foliar versus root exposure). Surprisingly, co-exposure exhibited a significant enantioselective effect on Cd accumulation. During the tillering stage, Cd- R -DIN caused slight differences in Cd accumulation within plant tissues compared to single Cd, whereas S -DIN markedly reduced Cd accumulation. At the grain-filling and maturity stages, co-exposure decreased the accumulation of both Cd (18.8 %–40.2 %) and DIN (22.3 %–63.3 %). Notably, Cd accumulation in grain under Cd- R -DIN (4.5 × 10 -3 mg/kg) was 3-fold higher than under Cd- S -DIN (1.5 × 10 -3 mg/kg), aligning with a 1.58-fold higher R -DIN accumulation. This indicated that standard doses of DIN (0.06 kg active ingredient/hectare), particularly S -DIN, mitigated Cd bioavailability and root-to-grain translocation. Spectroscopic analyses excluded the enantioselective possibility of direct chemical complexation between DIN and Cd. Proteomics identified ABC transporters as key regulators, with OsABCG14 being upregulated (17.6 %) by Cd- R -DIN but downregulated (18.4 %) by Cd- S -DIN. Molecular dynamics simulations revealed that OsABCG14 exhibited a more favorable binding ability with Cd- R -DIN (−60.1 kJ/mol) than with Cd- S -DIN (−43.5 kJ/mol). Heterologous expression in yeast confirmed that strains overexpressing OsABCG14 exhibited increased sensitivity to Cd- R -DIN, validating enantioselective transport. These findings highlight that the targeted application of specific pesticide enantiomers (e.g., S -DIN) could serve as a dual-action strategy for pest management and heavy metal mitigation in co-contaminated agricultural environments. Download: Download high-res image (274KB) Download: Download full-size image
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高影响因子
ACS Agricultural Science & Technology
·
2025
·
IF 2.9
Bin Sheng,Ying Zhang,Kexin Yang,Xiaobin Wen,Zhihao Lin,Zhijian Wu,Yaowei Zhang,Jie Zhou,Xueling Ye,Miao Wang,Ge Chen,Guangyang Liu,Xiaomin Xu,Xin Yang,Bining Jiao,Donghui Xu
Neonicotinoid insecticides face a number of challenges in improving the efficacy and mitigating resistance in Bemisia tabaci. The zeolitic imidazolate framework (ZIF) in metal–organic frameworks (MOFs) has attracted much attention in the field of nanopesticide preparation and controlled release due to its simple preparation. However, monometallic ZIFs have a simple structure and low loading capacity. To solve this problem, we doped Fe into ZIF-L and successfully synthesized a cross-stacked bimetallic ZIF nanocarrier, which can effectively load and release dinotefuran (DNF). Subsequent preparation of Zn-Fe-ZIF@DNF@MPN after encapsulation using metal–phenolic networks (MPNs) increased the DNF loading to 23.74% and achieved >99% release within 24 h. Zn-Fe-ZIF@DNF@MPN has greater resistance to UV light, retention on vegetable leaves, and resistance to rainwater washout. Dynamic residue analysis confirmed its effectiveness and persistence. In addition, it achieved 86.95% mortality against Bemisia tabaci without inhibiting cabbage seed germination. This work is of strategic importance for the development of a functional and environmentally friendly smart nanopesticide.
研究领域:
农药学
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材料科学
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昆虫毒理学
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环境科学
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2023
·
IF 6.8
Yirong Zhang,Zhenchao Tan,Kaikai Qin,Chenglan Liu
Pesticides and heavy metals commonly coexist in soil. In this study, the influence of Cd and Cu on the toxicity of rac -dinotefuran and the enantioselective behavior of dinotefuran enantiomers in soil-earthworm microcosms were investigated. The acute toxic tests showed that S -dinotefuran has higher toxic than that of R -dinotefuran. The rac -dinotefuran and Cd has an antagonistic effect on earthworms, and the Cu and rac -dinotefuran has a synergistic effect. Earthworms maybe promoted the enantioselective behavior of dinotefuran in soil. Co-exposure to Cd or Cu inhibited the dissipation of dinotefuran enantiomers ( S -dinotefuran and R -enantiomers), and slightly reduced the enantioselectivity in soil. The earthworms were found to be preferentially enriched with S -dinotefuran. However, Cd or Cu attenuated the accumulation of dinotefuran enantiomers in earthworms and decreased the enantioselectivity. The effect of Cd and Cu on the environmental behaviors of dinotefuran enantiomers were correlated positively with the dose of Cd/Cu. These results showed that Cd and Cu alter the environmental behaviors and the toxicity of dinotefuran enantiomers in soil-earthworm microcosms. Thus, the influence of coexistent heavy metals on the ecological risk assessment of chiral pesticides should be considered.
研究领域:
环境科学
·
毒理学
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yue Yang,Qunyan Wei,Tong Zou,Yulin Kong,Linfeng Su,Dian Ma,Yude Wang
Developing dinotefuran (DNF) sensing probe is crucial for the monitoring of DNF residues. A dual-emission ratiometric fluorescent probe of sulfur-doped carbon quantum dots (S-CQDs) and copper nanoclusters (CuNCs) hybrid (S-CQDs/CuNCs) were developed for sensitive and selective detection of DNF. The fluorescent signals of CuNCs were quenched in the presence of DNF, while the fluorescent signals of S-CQDs enhanced after the addition of DNF. The ratiometric fluorescent performance of as-developed probe was mainly based on the inner filter effect (IFE) between S-CQDs and CuNCs. Interestingly, the aggregation of CuNCs occurred after the addition of DNF, reducing the IFE effect between S-CQDs and CuNCs, leading to the fluorescent intensity of S-CQDs recovered and CuNCs fluorescence decayed. The ratiometric fluorescent performance of S-CQDs/CuNCs illustrated a good sensitivity and selectivity ranging from 10 μM and 500 μM, with a low detection limit of 7.04 μM. Importantly, real samples investigations implied the good application potential of S-CQDs/CuNCs in DNF monitoring.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ao Liang,Jie Hu,Zuhan Zhao,Xiangsong Li,Jian Pu,Jizhi Yang,Liping Yu,Xuegui Wang
Sogatella furcifera ( S. furcifera ) is a serious pest of rice in China. Excessive use of conventional insecticides has led to environmental pollution and other associated issues. The rapid advancement of nanotechnology offers new perspectives for developing innovative pest management strategies. Therefore, we designed a nanopesticide system in which dinotefuran (DNF) was encapsulated within chitosan (CS) and further integrated with ZnO quantum dots (ZnO QDs). The resulting formulation (DNF@CS-P-ZnO) displayed an average particle size of 33.3 nm and a loading efficiency of 17.74 %. Under acidic conditions (pH 5), about 74.93 % of DNF was released within 96 h, showing a controlled release profile. Bioassays confirmed high insecticidal activity, with mortality reaching 93.67 % after 4 d. Mechanistic analyses indicated that the nanoformulation inhibited cytochrome P450 activity and downregulated resistance-related genes ( CYP417A2 , CYP439A3 , CYP4CE3 ), thereby enhancing susceptibility to insecticides. Additionally, the formulation exhibited effective translocation within both pests and rice plants, improved photostability, enhanced wettability, and minimal impact on non-target organisms. Overall, DNF@CS-P-ZnO represents a promising nanopesticide platform that improves pesticide efficacy while reducing environmental risks, offering potential for sustainable rice pest control.
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Caiping Hu,Usman Farooq,Baoshuai Li,Dalu Yu,Bo Zhou,Jinxiao Wang,Weifeng Chen,Zhichong Qi
As an effective soil amendment, biochar can reduce the adverse effects and environmental impacts of neonicotinoid pesticides. Rhamnolipid, an active and common biosurfactant in soil systems, may alter the co-transport behaviors of biochar colloids and pesticides. Nevertheless, biosurfactant-mediated changes in pesticide mobility in soils affected by biochar colloids are currently lacking. Herein, the single or combined effects of biochar colloids and rhamnolipid on the transport properties of dinotefuran (DTF, a typical neonicotinoid pesticide) in soil matrix were investigated. When the influents contained biochar colloids, the DTF transport was reduced, primarily via colloid-associated DTF deposition and pesticide bound to the deposited colloids on porous media surfaces. Meanwhile, rhamnolipid also restrained DTF mobility because of its bridging effect (i.e., this biosurfactant mediated DTF-soil binding through molecular bridging). However, rhamnolipid notably diminished the suppressed influences of biochar colloids on DTF transport because of the decrease of colloid-associated DTF retention and the high mobility of free DTF. Moreover, the degree of rhamnolipid's weakened effects was related to the solution pH values (i.e., the influences declined as the pH values increased). Because rhamnolipid exhibited greater inhibition effects on the binding abilities of colloids to pesticide molecules under lower pH conditions (pH 5.0), leading to more free DTF mobility. The results underscore the critical roles of ubiquitous biosurfactants in the fate and risks of pesticides when biochar is used as an amendment for contaminated soil.
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高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Cai Xiao-Yu,Xu Man,Zhu Yu-Xuan,Shi Ying,Wang Hong-Wei
Neonicotinoids (NEOs) are the most widely used insecticides in the world and pose a serious threat to aquatic ecosystems. The combined use of free-floating aquatic plants and associated microorganisms has a tremendous potential for remediating water contaminated by pesticides. The aim of this study was to determine whether plant growth-promoting bacteria (PGPB) could enhance the phytoremediation efficiency of duckweed (Lemna aequinoctialis) in NEO-contaminated water. A total of 18 different bacteria were isolated from pesticide-stressed agricultural soil. One of the isolates, Pseudomonas monteilii FC02, exhibited an excellent ability to promote duckweed growth and was selected for the NEO removal experiment. The influence of strain FC02 inoculation on the accumulation of three typical NEOs (dinotefuran, thiacloprid, and imidaclothiz) in plant tissues, the removal efficiency in water, and plant growth parameters were evaluated during the 14-day experimental period. The results showed that strain FC02 inoculation significantly (p < 0.05) increased plant biomass production and NEO accumulation in plant tissues. The maximum NEO removal efficiencies were observed in the inoculated duckweed treatment after 14 days, with 92.23, 87.75, and 96.42% for dinotefuran, thiacloprid, and imidaclothiz, respectively. This study offers a novel view on the bioremediation of NEOs in aquatic environments by a PGPB–duckweed partnership.
研究领域:
环境科学
·
微生物学
·
植物学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Qinghua Yao,Deseng Su,Minmin Huang,Yunyun Zheng,Meizhen Chen,Qiu Lin,Hui Xu,Shaoxiao Zeng
Sensitive, simple and quick methods for determination of dinotefuran , cyromazine , and the metabolites 1-methyl-3-(tetrahydro-3-furylmethyl) guanidine (DN), 1-methyl-3-(tetrahydro-3-furylmethyl) urea (UF), and melamine in Agrocybe aegerita were established and validated by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The dissipation and residues of these compounds in A. aegerita were investigated. The field results showed that dinotefuran and cyromazine dissipated rapidly with half-lives ranging from 24.9 h to 38.5 h. The terminal residues of total dinotefuran (the sum of dinotefuran and its metabolites), cyromazine, and melamine in fresh mushroom samples collected with sampling intervals of 3 d to 6 d ranged from 0.089 to 0.652 mg/kg, 0.593 to 2.054 mg/kg, and 0.002 to 0.005 mg/kg, respectively. During the drying processes of A. aegerita, the processing factors of dinotefuran, cyromazine, DN, UF, and melamine were 0.028, 0.012, 0.301, 0.002, and 0.001, respectively. Given that cyromazine and dinotefuran are not registered for use on A. aegerita , dietary risk was assessed. Based on a consumption survey, risk quotient (RQ) values significantly below 1 implied that the risk of A. aegerita consumption was acceptable, although the risk for children was relatively higher. The findings could provide valuable guidance for the appropriate use of these insecticides in A. aegerita cultivation and serve as a scientific reference for setting corresponding maximum residue limits (MRL) in China.
研究领域:
食品科学
·
环境毒理学
·
分析化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）