MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Kangzhe Cao,Jinjin Fu,Fan Zhang,Jiakun Zhang,Fei Sun,Yanhao Qin,Jun-Tao Cao,Yong Lei,Huiqiao Liu
Aberrant microRNA (miRNA) expression is a critical biomarker for various diseases, yet its low abundance in biofluids poses significant detection challenges, limiting clinical utility. To overcome the sensitivity limitations of conventional methods, this paper developed a novel liposome-assisted electrochemical surface-enhanced Raman scattering (EC-SERS) sensor for ultrasensitive miRNA detection. This platform uniquely integrates three synergistic amplification strategies: (1) catalytic hairpin assembly (CHA) for exponential target miRNA recycling and capture, (2) high-capacity liposomal nanocarriers densely loaded with cationic Rhodamine 6G (R6G) SERS reporters, and (3) a low-cost silver nanowire-coated screen-printed electrode (AgNW-SPE) generating intense electromagnetic fields under electrochemical modulation. Target miRNA initiates CHA cycling, immobilizing numerous DNA-tagged liposome probes on the SPE. Subsequent electrostatic attraction between the anionic AgNW-SPE and cationic R6G released from lysed liposomes concentrates the reporters at electromagnetic hotspots on the nanowires. This synergistic cascade enables ultrasensitive and specific detection. The EC-SERS sensor achieves a remarkably low detection limit of 28 fM and a wide linear range (1.0 × 10 −13 to 1.0 × 10 −9 M) for target miRNA, with high specificity against mismatched sequences. Demonstrated in complex biological serum samples, this robust and cost-effective platform provides a powerful tool for highly sensitive miRNA profiling. It holds significant potential for advancing biomarker discovery, enabling early diagnosis of disease, and facilitating personalized therapeutic strategies within precision medicine.
研究领域:
生物分析化学
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纳米技术
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分子诊断学
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生物传感器
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RNA生物学
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生物医学工程
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产品关联
DOI
高影响因子
Water
·
2020
·Vol. 12
·期 2
·595
·
IF 2.544
Gaogui Jing,Shuai Ren,Yuesheng Gao,Wei Sun,Zhiyong Gao
Mineral processing wastewater contains large amounts of reagents which can lead to severe environmental problems, such as high chemical oxygen demand (COD). Inspired by the wastewater treatment in such industries as those of textiles, food, and petrochemistry, in the present work, electrocoagulation (EC) is applied for the first time to explore its feasibility in the treatment of wastewater with an initial COD of 424.29 mg/L from a Pb/Zn sulfide mineral flotation plant and its effect on water reuse. Typical parameters, such as anode materials, current density, initial pH, and additives, were characterized to evaluate the performance of the EC method. The results showed that, under optimal conditions, i.e., iron anode, pH 7.1, electrolysis time 70 min, 19.23 mA/cm2current density, and 4.1 g/L activated carbon, the initial COD can be reduced to 72.9 mg/L, corresponding to a removal rate of 82.8%. In addition, compared with the untreated wastewater, EC-treated wastewater was found to benefit the recovery of galena and sphalerite, with galena recovery increasing from 25.01% to 36.06% and sphalerite recovery increasing from 59.99% to 65.33%. This study confirmed that EC is a promising method for the treatment and reuse of high-COD-containing wastewater in the mining industry, and it possesses great potential for wide industrial applications.Keywords:mineral processing;wastewater treatment;flotation;electrocoagulation (EC);chemical oxygen demand (COD)
研究领域:
环境科学
·
矿业工程
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产品关联
DOI
高影响因子
Endocrine Connections
·
2019
·Vol. 8
·期 1
·8-19
·
IF 2.474
Shan Wu,Jianjun Zhou,Jing Guo,Zhan Hua,Jianchen Li,Zai Wang
Angiogenesis has a pivotal role in the growth and metastasis of pancreatic neuroendocrine tumors (PNETs). Apatinib inhibits angiogenesis as a highly selective KDR inhibitor and has been used to treat advanced gastric cancer and malignancies in clinical settings. However, the efficacy of apatinib in PNETs remains unclear. The aim of this study was to compare the antitumor efficacy of apatinib with that of the standard PNET drug sunitinib in our subcutaneous and liver metastasis models of insulinoma and non-functional PNET. Our results revealed that apatinib had a generally comparable or even superior antitumor effect to that of sunitinib on primary PNET, and it inhibited angiogenesis without directly causing tumor cell cytotoxicity. Apatinib inhibited the tumor in a dose-dependent manner, and the high dose was well tolerated in mice. We also found that the apatinib efficacy in liver metastasis models was cell-type (disease) selective. Although apatinib efficiently inhibited INR1G9-represented non-functional PNET liver metastasis, it led to the emergence of a hypoxic area in the INS-1-represented insulinoma and promoted liver metastasis. Our study demonstrated that apatinib has promise for clinical applications in certain malignant PNETs, and the application of anti-angiogenesis drugs to benign insulinomas may require careful consideration. Angiogenesis has a pivotal role in the growth and metastasis of pancreatic neuroendocrine tumors (PNETs). Apatinib inhibits angiogenesis as a highly selective KDR inhibitor and has been used to treat advanced gastric cancer and malignancies in clinical settings. However, the efficacy of apatinib in PNETs remains unclear. The aim of this study was to compare the antitumor efficacy of apatinib with that of the standard PNET drug sunitinib in our subcutaneous and liver metastasis models of insulinoma and non-functional PNET. Our results revealed that apatinib had a generally comparable or even superior antitumor effect to that of sunitinib on primary PNET, and it inhibited angiogenesis without directly causing tumor cell cytotoxicity. Apatinib inhibited the tumor in a dose-dependent manner, and the high dose was well tolerated in mice. We also found that the apatinib efficacy in liver metastasis models was cell-type (disease) selective. Although apatinib efficiently inhibited INR1G9-represented non-functional PNET liver metastasis, it led to the emergence of a hypoxic area in the INS-1-represented insulinoma and promoted liver metastasis. Our study demonstrated that apatinib has promise for clinical applications in certain malignant PNETs, and the application of anti-angiogenesis drugs to benign insulinomas may require careful consideration.
研究领域:
肿瘤学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
EUKARYOTIC CELL
·
2015
·Vol. 14
·期 4
·
IF 2.82
Yanfang Shang,Peilin Chen,Yixiong Chen,Yuzhen Lu,Chengshu Wang
Two-component signaling pathways generally include sensor histidine kinases and response regulators. We identified an ortholog of the response regulator protein Skn7 in the insect-pathogenic fungus Metarhizium robertsii, which we named MrSkn7. Gene deletion assays and functional characterizations indicated that MrSkn7 functions as a transcription factor. The MrSkn7 null mutant of M. robertsii lost the ability to sporulate and had defects in cell wall biosynthesis but was not sensitive to oxidative and osmotic stresses compared to the wild type. However, the mutant was able to produce spores under salt stress. Insect bioassays using these spores showed that the virulence of the mutant was significantly impaired compared to that of the wild type due to the failures to form the infection structure appressorium and evade host immunity. In particular, deletion of MrSkn7 triggered cell autolysis with typical features such as cell vacuolization, downregulation of repressor genes, and upregulation of autolysis-related genes such as extracellular chitinases and proteases. Promoter binding assays confirmed that MrSkn7 could directly or indirectly control different putative target genes. Taken together, the results of this study help us understand the functional divergence of Skn7 orthologs as well as the mechanisms underlying the development and control of virulence in insect-pathogenic fungi.
研究领域:
真菌学
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分子生物学
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真菌遗传学
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昆虫病理学
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微生物致病机制
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Weixia Lv,Caijian Zhu,Jun Chen,Caixia Ou,Qian Zhang,Shengwen Zhong
In this work, the solvent and lithium salt are modified simultaneously to improve the low temperature performance of the electrolyte. The butyl acrylate (BA) with low melting point and high dielectric constant is combined with the ethylene carbonate (EC) with high dielectric constant and excellent film-forming property, which used as mixed additives. On the other hand, the LiBF 4 with small ion size and large ion mobility is chose to mixed with LiPF 6 with best comprehensive performance, which are used as mixed lithium salts. The BA + EC + LBF mixed additives improves the solubility, conductivity and melting point of the electrolyte, and effectively improves the composition, thickness and uniformity of the CEI film, which together increased the capacity and voltage platform of the batteries at low temperatures. The discharge capacities of BA16%+EC10%+0.3 M LBF battery at −10, −20, −30 and −40 °C are 173.1, 156.0, 147.0, and 119.3 mAh/g respectively, which are much higher than the batteries with the electrolyte without adding additives (122.0, 107.8, 95.7, and 74.3 mAh/g respectively). Furthermore, BA + EC + LBF can form a porous and thin LiF CEI film on the cathode surface, which is very favorable for transport of Li + , further improve the behaviors of the batteries at different low temperatures. Even in the case of charging and discharging at −40 °C, its discharge capacity can be stably maintained at 85 mAh/g. The BA + EC + LBF mixed additives used in this work as an effective strategy to improve the comprehensive behaviors of the battery at low temperatures, which would provide a new insight to design high performance of low temperature electrolyte.
研究领域:
材料科学
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电化学
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产品关联
DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 54
·34921-34930
·
IF 4.036
Zhiqian Liu,Linli Qin,Sijia Liu,Jing Zhang,Junhua Wu,Xinquan Liang
Electrospun fiber membranes have great potential in the field of air filtration because of their high porosity and small pore size. Conventional air filtration membranes are hydrophilic, leading to weak moisture-barrier properties, which hinders their application in high-humidity environments. In this study, eugenol was added to polyvinyl alcohol and ethyl cellulose (EC) for electrospinning and electrospraying, respectively, of superhydrophobic bilayer composite fiber membranes to efficiently filter particulate matter (PM) in air. Owing to its surface microstructure, electrosprayed EC increased the water contact angle of the PVA membrane from 142.8 to 151.1°. More importantly, the composite air-filter membrane showed a low filtration pressure drop (168.1 Pa) and exhibited high filtration efficiencies of 99.74 and 99.77% for PM1.0 and PM2.5, respectively, and their respective quality factors were 0.0351 and 0.0358 Pa−1. At the same time, the filtration performance of the air filtration membrane remained above 99% at high air humidity. This work reports composite membranes that can effectively capture PM of various sizes and thus may provide a reference for the manufacturing of green air filters for high-humidity environments.
研究领域:
材料科学
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环境科学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL TECHNOLOGY
·
2023
·
IF 2.8
Na Sun,Xianglian Wang,Zhanmeng Liu
In this study, electrochemically generated free chlorine (EC/Cl2) was activated by UV irradiation with a light emitting diode (LED) lamp at 275 nm to degrade acetaminophen (AAP, 2 μM) in aqueous solution. The potential at a RuO2-IrO2/Ti plate anode was set at 1.5 V vs. the Ag/AgCl electrode. Chlorine was in situ generated in the presence of Cl杴 at the anode and then it was transformed into various active species such as 晑OH and reactive chlorine species (RCS) under UV-LED irradiation. The degradation of AAP was investigated using batch tests, evaluating the influence of different experimental conditions such as NaCl concentration, phosphate buffer saline concentration, irradiation time and solution pH, keeping constant the UV-LED power and temperature. Results show that AAP could be completely degraded by the hybrid process with a high mineralization ratio (73%), and the degradation process followed a pseudo-first-order kinetics. The value of the Electric Energy per Order (EEO) = 1.272 kWh m杴3 order?, which is lower than the energy consumption of some other UV-based processes for AAP degradation. Adding 1 mM HCO3杴 ions slightly decreased the rate of AAP degradation. Luminescent bacteria experiment revealed that the acute toxicity of the reacted solution could be greatly reduced and the ecological risk was effectively abated. The scavenging assay shows that RCS plays a key role in the AAP degradation. The intermediate products were identified, and possible degradation routes were proposed. The system can advantageously replace conventional UV mercury lamp based ones in the degradation of microorganic pollutants.
研究领域:
环境科学
·
水处理工程
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光催化降解
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DOI
PubMed
高影响因子
ChemCatChem
·
2024
·
IF 3.8
Li Zhang,Haiyan Xu,Xiaofan Wu,Yitong Li,Yuan Tao,Zedu Huang,Fener Chen
Graphical Diversification of commercially available 3’-amino-3’-deoxythymidine via nucleoside phosphorylase Ec PPNP- and/or nucleoside 2’-deoxyribosyltransferase Ll NDT-catalyzed transglycosylation has been demonstrated as a generally applicable, straightforward means to access a variety of valuable 3’-amino containing nucleosides in good yields. The synthetic utility was further showcased by the successful synthesis of 3’-amino-2’,3’-dideoxy-5-bromouridine in 29% isolated yield at a preparative scale. A systematic study on the enzymatic transglycosylation of 3′-amino nucleosides has been reported. Using the commercially available 3′-amino-3′-deoxythymidine as the sugar donor, 13 pyrimidine and purine nucleobases were shown as the viable sugar acceptors in the nucleoside phosphorylase Ec PPNP- and/or nucleoside 2′-deoxyribosyltransferase Ll NDT-catalyzed reactions, furnishing the corresponding 3′-amino containing nucleosides in 23%–73% NMR yields at an analytical scale. The synthetic utility of this diversification approach was further showcased by the successful synthesis of 3′-amino-2′,3′-dideoxy-5-bromouridine in 29% isolated yield at a preparative scale. The current work demonstrates the transglycosylation activity of Ec PPNP for the first time, expands the substrate scope of Ll NDT, and provides an efficient, direct access to various 3′-amino nucleosides of high value.
研究领域:
生物化学
·
药物化学
·
酶学
✓ 已验证
DOI
高影响因子
Journal of Environmental Sciences
·
2023
·
IF 6.9
Yue Liu,Junjie Liu,Ji Xiao,Jing Ye,Lixu Guo,Caiqing Yan
Carbonaceous aerosol, including organic carbon (OC) and elemental carbon (EC), has significant influence on human health, air quality and climate change. Accurate measurement of carbonaceous aerosol is essential to reduce the uncertainty of radiative forcing estimation and source apportionment. The accurate separation of OC and EC is controversial due to the charring of OC. Therefore, the development of reference materials (RM) for the validation of OC/EC separation is an important basis for further study. Previous RMs were mainly based on ambient air sampling, which could not provide traceability of OC and EC concentration. To develop traceable RMs with known OC/EC contents, our study applied an improved aerosol generation and mixing technique, providing uniform deposition of particles on quartz filters. To generate OC aerosol with similar pyrolytic property of ambient aerosol, both water soluble organic carbon (WSOC) and water insoluble organic carbon (WIOC) were used, and amorphous carbon was selected for EC surrogate. The RMs were analyzed using different protocols. The homogeneity within the filter was validated, reaching below 2 %. The long-term stability of RMs has been validated with RSD ranged from 1.7–3.2 %. Good correlation was observed between nominal concentration of RMs with measured concentration by two protocols, while the difference of EC concentration was within 20 %. The results indicated that the newly developed RMs were acceptable for the calibration of OC and EC, which could improve the accuracy of carbonaceous aerosol measurement. Moreover, the laboratory-generated EC-RMs could be suitable for the calibration of equivalent BC concentration by Aethalometers.
研究领域:
环境科学
·
大气化学
·
分析化学
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Yiping He,Tangli Chen,Yuelin Zhang,Yule Shi,Liang Yu,Yurui Hao,Yuchen Tan,Qinyu Shi,Zihao Cai,Hao Liu,Chengshan Xu,Nao Kobayashi,Yue Qiu,Gaolong Zhu,Shigeaki Yamazaki,Junxian Hou,Xuning Feng,Languang Lu,Terada Junpei,Minggao Ouyang
High-nickel lithium-ion batteries are known for their high energy density, but face severe safety challenges. The initial heat and gas accumulation originate from the highly reductive lithiated anode at the anode-electrolyte interface within the cell, which finally triggers the intensive exothermic reactions. Herein, an ethylene carbonate-less (EC-less) electrolyte endowed with a bianionic coordination solvation structure is designed, which significantly enhances the thermal stability of LiNi0.8Co0.1Mn0.1O2|graphite (NCM811|Gr) pouch cells by mediating anode-electrolyte interfacial reactions. This bianionic structured EC-less electrolyte demonstrates superior reductive stability against the highly reactive lithiated anode, as evidenced by its elevated lowest unoccupied molecular orbital (LUMO) energy level. Material-level thermal analysis confirms that EC-less electrolyte markedly manipulates the electrolyte reduction reactions, achieving a 29.7% reduction in heat release (−225.5 J g −1 ) relative to conventional electrolyte (−320.8 J g −1 ). Further validation using 1 Ah NCM811|Gr pouch cells demonstrates that EC-less electrolyte enables the cell to maintain thermal inertness at 180 °C for 40 min, whereas a conventional cell experiences intensive thermal runaway at 160 °C. Furthermore, EC-less electrolyte facilitates the formation of an inorganic-rich interphase, and the pouch cell exhibits excellent electrochemical performance. This research highlights the potential of interfacial reaction engineering for safer high-nickel lithium batteries.
研究领域:
储能
·
电化学
·
材料科学
·
电池技术
✓ 已验证
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2021
·
IF 4.598
Shuangping Xu,Wenqiang Ma,Hailiang Zhou,Yushu Zhang,Hongge Jia,Jingyu Xu,Pengfei Jiang,Xintian Wang,Wenwen Zhao
Hybrid membranes containing different loadings of butadiene-bridged polymethylsiloxane (BBPMS) in ethyl cellulose (EC) were prepared by an one-pot blending method from a non-isolated solution of polymethylhydrosiloxane (PMHS) reaction with butadiene, and a solution of EC in THF. The semi-crosslinked BBPMS and the BBPMS/EC hybrid membranes having good compatibility were characterized by Fourier transfer infrared (FTIR) and Scanning electron microscopy (SEM). The BBPMS/EC hybrid membranes exhibited good membrane forming ability, heat resistance and mechanical properties. It was worth mentioning that the BBPMS/EC blended membranes exhibit good selectivity for CO 2 /N 2 and O 2 /N 2 . Specifically, the blended membrane (BBPMS/EC-5:5) showed the highest ideal selectivities for CO 2 /N 2 and O 2 /N 2 were 25.5 (at a CO 2 permeability of 175 Barrer) and 4.85 (at a O 2 permeability of 33.2 Barrer), respectively. Compared to the pure EC membrane, the ideal CO 2 /N 4 selectivity and the ideal O 2 /N 2 selectivity of the blended membrane (BBPMS/EC-5:5) were increased by 41.8% and 53.0%, respectively. The modification strategy using semi-crosslinked polysiloxanes to enhance gas transfer of EC is promising for fabrication of high performance CO 2 and O 2 separation membranes.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Muzammal Hussain,Mulenga Kalulu,Zaheer Ahmad,Onome Ejeromedoghene,Guodong Fu
Ethyl cellulose (EC), polyvinylpyrrolidone (PVP), and phosphomolybdic acid (PMoA) were the components of a new photochromic hybrid film composed of heteropoly acids (abbreviated EC-PVP/PMoA), created by solvent evaporation. The EC-PVP/PMoA mechanism, visible light photochromic behaviors, and microstructure were closely studied by transmission electron microscopy (TEM), atomic force microscopy (AFM), ultraviolet-visible (UV–visible) spectroscopy, X-ray photo electronics (XPS), and Fourier transmission infrared spectroscopy (FTIR). FTIR spectra show that neither the core structures of EC, PVP, nor the Keggin structure of PMoA in the EC-PVP/PMoA composite hybrid film were damaged during fabrication. In the presence of oxygen, the yellowish transparent hybrid film exhibited reversible photochromism and significant photochromic reactivity, becoming blue in the presence of visible light. Upon heating the film at 90 °C for 1 h its photoresponsive properties were enhanced, making the blue color more vibrant and stable due to polyethylene glycol (PEG). The XPS spectra demonstrated a proton transfer procedure during the photochromic process, and the presence of Mo +5 species indicated a photoreduction interaction between the PMoA particles and the EC-PVP matrix. To the best of our knowledge, this is the first EC-PVP combined hybrid film with POMs with promising prospects for solar UV detection and smart glass windows applications.
研究领域:
材料科学
·
光化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）