Food Analytical Methods
·
2023
·
IF 2.9
Qiao Fanglin,Chen Yin,Fei Yunjie,Jiang Yanjun,Xiong Xiaohui,Liu Yuanjian
Due to the complex characteristics of food matrix, false positives may occur when using traditional methods to detect aflatoxin B1 (AFB1). In this paper, we utilize the advantages of closed bipolar electrode (BPE) that can effectively avoid the impact of complex food matrices on signal collection, and design a biosensor based on immune competition to detect highly toxic AFB1. The biosensor uses cathode of closed BPE as a functional sensing interface and anode as a signal collection interface. On the functional sensing interface, AFB1 connects to the monoclonal antibody at the top of DNA tetrahedron structure. Under the action of horseradish peroxidase (HRP), 4-chloro-1-naphthol (4-CN) is catalyzed by hydrogen peroxide (H 2 O 2 ) to generate precipitations (4-CD). AFB1 competes with HRP-AFB1 to bind monoclonal antibody, resulting in a decrease in the content of HRP involved in catalytic oxidation reactions and a synchronous reduction in precipitations produced, causing an increase in the intensity of [Ru(bpy) 3 ] 2+ /TPA system electrochemiluminescence (ECL) on the BPE anode. Analysis shows that the relative deviation between the detection results and ELISA is between −4.5 and 9.8%, and there is no significant difference in the performance of the two in detecting AFB1. The BPE-ECL sensor exhibits high sensitivity and specificity in detecting AFB1, with a linear range of 0.01–40 ng mL −1 and a detection limit of 3 pg mL −1 . In the future, it can be applied to the detection of other toxins, with broad application prospects.
研究领域:
食品安全检测
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生物传感
·
分析化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Xiaoyue Zhang,Yue Jia,Nuo Zhang,Dan Wu,Hongmin Ma,Xiang Ren,Huangxian Ju,Qin Wei
The unique optoelectronic and tunable luminescent characteristics of copper nanoclusters (Cu NCs) make them extremely promising as luminophores. However, the limited luminescence intensity and stability of Cu NCs have restricted their application in the field of electrochemiluminescence (ECL). Herein, a self-assembly-induced enhancement strategy was successfully employed to enhance the cathodic ECL performance of flexible ligand-stabilized Cu NCs. Specifically, Cu NCs form ordered sheetlike structures through intermolecular force. The restriction of ligand torsion in this self-assembled structure leads to a significant improvement in the ECL properties of the Cu NCs. Experimental results demonstrate that the assembled nanoscale Cu NC sheets exhibit an approximately three-fold increase in cathodic ECL emission compared to the dispersed state of Cu NCs. Furthermore, assembled nanoscale Cu NCs sheets were utilized as signal probes in conjunction with a specific short peptide derived from the catalytic structural domain of matrix metalloproteinase 14 (MMP 14) as the identification probe, thereby establishing a split-type ECL sensing platform for the quantification of NMP 14. The investigation has revealed the exceptional performance of assembled nanoscale Cu NCs sheets in ECL analysis, thus positioning them as novel and promising signal probes with significant potential in the field of sensing.
研究领域:
纳米技术
·
分析化学
·
生物传感
·
材料科学
·
电化学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 15
·3702-3707
·
IF 3.864
Jun-Tao Cao,Fu-Rao Liu,Fang Hou,Juan Peng,Shu-Wei Ren,Yan-Ming Liu
The cathodic electrochemiluminescence (ECL) behaviour of nontoxic MoS2 quantum dots (QDs) was studied for the first time using potassium peroxydisulfate as the co-reactant. Ag-PAMAM NCs, serving as difunctional tags for quenching and enhancing ECL of MoS2-reduced graphene oxide composites, were introduced into the ECL detection system for signal amplification. By modulating the interparticle distance between MoS2 QDs and Ag-PAMAM NCs, the ECL quenching from resonance energy transfer and the ECL enhancement from surface plasma resonance were realized. Coupling the good ECL performance of MoS2 QDs with the excellent ECL quenching and enhancement effects of Ag-PAMAM NCs, a novel MoS2 QDs-based ECL biosensing platform for sensitive detection of microRNA-21 was achieved with a detection limit of 0.20 fmol L−1 (S/N = 3). This method was successfully applied to the determination of microRNA-21 in human serum samples with recoveries of 90.0–110.0%, suggesting great potential for its applications in biological and chemical analysis.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2012
·
IF 5.602
Jing Wang,Wei-Wei Zhao,Hong Zhou,Jing-Juan Xu,Hong-Yuan Chen
In this work, we firstly enunciated the presence of synergy effect between the electron and energy transfer in an electrochemiluminescence (ECL) system involving the CdS NCs and Au NPs, based on which an amplified ECL biosensor was constructed for sensitive detection of DNA–protein interactions. Specifically, Au NPs modified on electrode, following by assembled probe DNA . Then the modified GCE was hybridized with SiO 2 @CdS/DNA conjugates. With DNA duplex as a rigid spacer, Au NPs could not only accelerate the electron transfer but also produce the surface plasma resonance upon excited ECL of CdS NCs. However, the enhanced ECL could be greatly suppressed by the binding of target protein. The prepared biosensor possesses excellent analytical performance with the linear range of target protein from 0.015 to 150 nM.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物传感器
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 13
·1560-1564
·
IF 2.073
Yang Li,Liu Yang,Zhewei Peng,Chengzhi Huang,Yuanfang Li
In this contribution, an ultra-sensitive electrochemiluminescence (ECL) sensor was proposed for dopamine (DA) detection based on ruthenium(II) complex (tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate, Ru(bpy)32+) functionalized Zn(II) metal–organic frameworks (Ru-MOFs). The Ru-MOFs, with large internal surface areas and abundant Ru(bpy)32+ encapsulated effectively, could act as a suitable ECL emitter in a DA detection system. The leakage of Ru molecules in the encapsulation system could be effectively prevented, which resulted in stable ECL signals and significantly improved the ECL performance. On the basis of the novel Ru-MOFs as efficient ECL tags, the as-prepared simple and label-free ECL sensor was employed to quantify dopamine, which showed an excellent linear response from 0.1 pM to 10 nM and a detection limit of 0.024 pM. The simple and sensitive strategy might be expanded to other applications in analysis and detection fields based on functional metal–organic frameworks.
研究领域:
分析化学
·
材料科学
·
电化学
·
传感器
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Lei Yu,Qiao Zhang,Qi Kang,Bin Zhang,Dazhong Shen,Guizheng Zou
The design and development of novel electrochemiluminescence (ECL) systems with near-infrared (NIR) emission beyond 800 nm are promising for ECL evolution, especially for improving the throughput of the spectrum-resolved multiplexing ECL assay and biological imaging. Herein a biocompatible and environmentally friendly luminophore, that is, the methionine-stabilized Au nanoclusters (Met-Au NCs), are proposed to achieve efficient aqueous ECL of ∼835 nm with triethanolamine as coreactant. The Met-Au NCs not only demonstrate a 75 times enhanced ECL compared with the traditional Au NCs with bovine serum albumin as a capping agent but also can be employed as ECL tags to label proteins with a methionine linker and enable a highly sensitive NIR ECL bioassay. A sandwich-type NIR ECL immunosensor is constructed with the Met-Au NCs as tags and α-fetoprotein (AFP) as a model analyte and exhibits a wide linearity range from 3 fg·mL–1 to 0.1 ng·mL–1 with a limit of detection of 1 fg mL–1 (S/N = 3) as well as desired selectivity.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Xueling Shan,Yuting Pan,Fanzhuo Dai,Xiaohui Chen,Wenchang Wang,Zhidong Chen
A sensitive electrochemiluminescence (ECL) sensor in solid-state for the detection of tetracycline (TET), based on carboxylated carbon nanotubes modified ZnO (CNT-COOHs/ZnO), was developed in this research. The ZnO, which worked as luminophore here, was an environment-friendly material. Resulted from its tremendously specific surface area, the loading amount of CNT-COOHs increased. The CNT-COOHs played a role of catalyzer to enhance the ECL intensity of CNT-COOHs/ZnO modified glass carbon electrode (GCE) in triethanolamine (TEOA) contained electrolyte. After adding TET in the solution, the ECL intensity significantly quenched. The ΔECL intensity showed a good correlation to the concentration of TET in the range of 1.0 × 10 –8 –1.0 × 10 –4 M and the detection limit achieved to 6.7 × 10 –9 M within a response time of 1.0 min. Furthermore, the proposed CNT-COOHs/ZnO based ECL sensor was applied for the detection of TET in fishpond water and exhibited a good accuracy. We believe our system supplied a potential platform for the detection of TET in real world.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Peipei Li,Luo Luo,Dan Cheng,Yan Sun,Youyu Zhang,Meiling Liu,Shouzhuo Yao
An electrochemiluminescence (ECL) sensor provides a sensitive and convenient method for early diagnosis of diseases; however, it is still a challenge to develop simple and sensitive sensing platforms based on efficient ECL signals and luminophore groups. Porphyrin-based metal–organic frameworks (MOFs) show great potential in ECL sensing; however, the mechanism and structure–activity relationship, as well as application, are rarely reported. Herein, hydrothermal reactions obtained porphyrin Zr-MOFs (PCN-222) with different specific surface areas, pore sizes, structures, and surface charge states by tuning the reaction time were developed, which served both as the ECL luminophore, coreaction promoter for S2O82–, and a connection in the ECL immunoassay. By progressively controlling the condition of the hydrothermal reaction, PCN-222 with large surface area-abundant micropores can be obtained, which has good conductivity and positively charged surfaces, obtaining excellent ECL performance. The ECL performance and the enhancement mechanism were investigated in detail. Using PCN-222-6h with the best ECL intensity as the immobilization matrix for the aptamer, a highly sensitive and selective assay for thrombin was developed. The decrease of the ECL signal was logarithmically linear with the concentration of thrombin in the range from 50 fg mL–1 to 100 pg mL–1 with a low detection limit of 2.48 fg/mL. This proposed strategy provides a brand new approach for tuning of the structures of MOFs as effective ECL signal probes, thus providing wider possibilities for effective ECL immunoassays in the detection of other biomarkers in diagnosis of diseases.
研究领域:
分析化学
·
材料科学
·
生物传感器
·
生物医学工程
✓ 已验证
DOI
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Maryam Allahyari,Emre Dokuzparmak,Arzum Erdem,Alper AKKAYA,Bahattin Tanyolac
Pseudomonas aeruginosa is a major pathogen responsible for severe infections, particularly in immunocompromised patients and those with chronic conditions. Its increasing resistance to antibiotics underscores the urgent need for effective detection systems. This study introduces a novel biosensor based on bacteriophage (M-PAP1) in combination with MWCNT-COOH modified SPE and its application for the sensitive identification of Pseudomonas aeruginosa . The phage provides high specificity and strong target binding, while the combined ECL and EIS modes offer complementary signal outputs, enhancing analytical reliability and reducing false responses. The ECL sensor achieved LoD of 0.755 CFU ml −1 and demonstrated a broad linear working range of 2.28 to 10 10 CFU ml −1 (R 2 : 0.9981). In artificial urine samples, the sensor demonstrated a recovery rate of 92% to 97%, indicating its effectiveness in real biological matrices. The dual functionality for both ECL and EIS measurements highlights the system's versatility and potential for real-time clinical applications. The system also exhibited exceptional selectivity and minimal interference from non-specific bacteria such as Escherichia coli and Pseudomonas putida and Pseudomonas fluorescens. Overall, the dual-mode strategy significantly strengthens diagnostic accuracy by enabling cross-validated detection signals, offering a robust platform for rapid monitoring of P. aeruginosa in the context of rising antimicrobial resistance.
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Fan Feng,Haicheng Gao,Yuan Xue,Qing Dong,Junfeng Zhai,Dan Li,Erkang Wang,Jin Wang
Exosomes, as extracellular vesicles, represent ideal biomarkers for liquid biopsy, enabling non-invasive, sensitive, and dynamic disease monitoring. Detection or profiling of exosomes are essential for advancing exosome-based cancer diagnostics or prognostic prediction. Here, we developed an enhanced electrochemiluminescence (ECL) biosensing platform that combines magnetic enrichment with catalytic hairpin assembly (CHA), an enzyme-free DNA amplification circuit triggered by the complementary sequences of CD63 aptamer (Apt), to achieve sensitive exosome detection. The enhanced ECL signal relies on a nanocomposite of a zirconium-based metal-organic framework (Zr-MOF) and water soluble gold nanoclusters (AuNCs), denoted as Zr-MOF/AuNCs. The platform exhibits a wide linear range (6.12 × 10² - 6.12 × 10⁷ particles/mL) and a low detection limit (187.4 particles/mL) for tumor-derived exosomes. Spike-recovery assays in complex serum matrices and high specificity confirm its robustness and clinical potential. By combining magnetic preconcentration, signal amplification via Zr-MOF and CHA, and Apt-mediated recognition, this work establishes a new paradigm for non-invasive cancer diagnostics.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Xuemei Pi,Rong Wang,Yiyu Guo,Xianwen Kan
l -Cysteine (L-Cys), one kind of aminothiol, ubiquitously exists in proteins and plays important roles in the food industry and biological processes. Herein, an electrochemiluminescence (ECL) sensor was constructed for the sensitive detection of L-Cys. As an ECL emitter, europium-porphyrin coordination polymer (Eu-PCP) was synthesized and subsequently modified with copper benzene-1,4-dicarboxylate (CuBDC) as a coreaction accelerator, which achieved high self-enhanced ECL responses. CuBDC on Eu-PCP was decomposed by the introduced L-Cys due to the coordination between Cu 2+ and L-Cys, resulting in the decrease of ECL signal. Besides, L-Cys could consume the co-reactant of K 2 S 2 O 8 to reduce the production of free radical SO 4 •– , further decrease ECL signal. Thus, this L-Cys coordination-driven decomposition of coreaction accelerator and consumption of co-reactant strategy was employed to doubly suppress the ECL signal for detecting L-Cys without additional recognition and amplification elements. The constructed ECL sensor exhibited a linear range of 1.0 × 10 −10 –5.0 × 10 −5 mol/L with a limit of detection of 2.0 × 10 −11 mol/L. This work has opened up new prospects for developing novel ECL sensing strategies in food safety monitoring and disease diagnosis.
研究领域:
分析化学
·
生物医学
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Yifan Dong,Mengyi Zheng,Bingjie Kong,Zehang You,Shuang Gao,Chaoyue Gu,Yuanhua Shao
Distinct from most electrochemiluminescence sensors that rely on elaborate materials, this study develops an immunosensor using simple materials for low-cost and highly sensitive biological analysis. We elucidated the mechanism of paradoxical amplification of gold nanoparticles (AuNPs), designed strategies of bidirectional catalysis, and expanded the application for multibiomarker detection. First, a new understanding of AuNPs’ catalytic role was established through investigating the inhibitory effects of five phenolic compounds on the luminol–H2O2 ECL system, and an AuNP-induced “paradoxical amplification” of phenolic electrochemiluminescence (ECL) inhibition was first identified. Contrary to the common view that AuNPs always enhance ECL, here, AuNPs amplified the inhibitory effect of phenolics, thereby reducing the ECL signal. Such a mechanism was validated via electrochemistry-mass spectrometry. Second, a methodological innovation is presented: a switch-type immunosensor with bidirectional catalytic amplification via dynamic conversion. Specifically, the same catalyst flexibly switches between enhancement and inhibition: horseradish peroxidase (HRP) and AuNPs first act synergistically to enhance the initial ECL signal of the luminol–H2O2 system; then, alkaline phosphatase serves as a “switch” and introduces phenolic compounds, triggering HRP and AuNPs to automatically convert into signal-inhibiting catalysts. This induces multiple signal attenuation via ECL energy resonance transfer, radical quenching, and mass transfer hindrance, leading to a rapid decrease in ECL response. Finally, a highly sensitive coupled enzyme-linked attenuated ECL immunoassay involving AuNPs was constructed and successfully applied to the specific detection of cardiac troponin I and erythropoietin with a wide linear range (from fg/mL to ng/mL), and a detection limit as low as fg/mL.
研究领域:
免疫学
·
分析化学
·
纳米生物技术
·
分子诊断
·
临床检验医学
·
转化医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）