MACROMOLECULAR MATERIALS AND ENGINEERING
·
2026
·Vol. 311
·期 9
·e70345
·
IF 5.2
Wei He,He Lv,Jingjing Feng,Wei Yi,Hongxi Wang,Deng-Guang Yu,Tao Yi
The development of food packaging materials with integrated antimicrobial and antioxidant functions is a crucial strategy for advancing the sustainability of the food packaging industry. In this study, we report a novel Janus-structured nanofibrous mat designed as a fish preservation gasket, fabricated using a modified trifluid electrospinning technique. The round side is composed of ethylcellulose and titanium dioxide, while the crescent side consists of sodium alginate, tea polyphenols, polyethylene oxide, and Triton X-100. The outer sheath solvent facilitates a stable and continuous process for the formation of Janus nanofibers. The resulting nanofibers exhibit a uniform linear morphology and a well-defined side-by-side Janus architecture. The incorporation of tea polyphenols significantly enhances the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. Furthermore, the mats demonstrate excellent antibacterial performance, achieving a 99.7% reduction against Bacillus subtilis WB800 and a 99.5% reduction against Escherichia coli DH5α within 0.5 h, which is attributed to the synergistic effect of tea polyphenols and titanium dioxide. Preservation tests demonstrate that the gaskets extended the shelf life of packaged fish to 9 days. Overall, this study establishes a new biomolecule-based nanofibrous platform with synergistic multifunctionality, offering a promising strategy for sustainable food preservation applications.
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DOI
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Jia-Cheng Qin,Xiao-Ping Tan,Ying-Jie Jin,Guang-Shuo Wang,Shen-Ye Qu,Peng-Fei Li,Gao-Xue Wang,Fei Ling
Fish iridoviruses, including the largemouth bass virus (LMBV) and Singapore grouper iridovirus (SGIV), pose severe threats to aquaculture industries, resulting in substantial economic losses. However, no effective treatments are currently available for these viruses, underscoring the critical need to develop high-efficacy therapeutic strategies. This study identified chlorchinaldol as a compound exhibiting significant anti-LMBV activity both in vitro and in vivo in largemouth bass ( Micropterus salmoides ). Results revealed that chlorchinaldol inhibited LMBV replication in Epithelioma papulosum cyprinid (EPC) cells with a half-maximal inhibitory concentration (IC 50 ) of 9.40 μM, while concurrently reducing viral titers and cytopathic effects (CPE). Mechanistic investigations demonstrated that chlorchinaldol does not directly disrupt LMBV virion integrity but interferes with viral adsorption to host cells and suppresses late-stage viral protein synthesis. Furthermore, chlorchinaldol exhibited both prophylactic and therapeutic efficacy, significantly mitigating LMBV-induced cellular damage. In in vivo experiments, chlorchinaldol administration reduced viral loads in infected organs, ameliorated histopathological damage, upregulated immune-related gene expression, and markedly enhanced survival rates in LMBV-infected largemouth bass. Notably, chlorchinaldol also demonstrated potent anti-SGIV activity in EPC cells, with an IC 50 of 10.01 μM and suppression of viral titers and CPE. These findings collectively establish chlorchinaldol, a quinoline derivative, as a broad-spectrum antiviral agent with substantial potential for aquaculture applications.
研究领域:
病毒学
·
水产养殖
·
药理学
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DOI
高影响因子
ACS Applied Energy Materials
·
2024
·
IF 5.4
Fancheng Meng,Peng Chen,Shulin Li,Na Cao,Sheng Cheng,Xiangfeng Wei,Jiangtao Di,Jiehua Liu
Compared with the rapidly developing lithium- and sodium-ion batteries, zinc-air batteries (ZABs) offer the advantages of safety and high energy density. Nevertheless, their applications in underwater conditions are currently limited due to the low oxygen levels in such environments. Drawing inspiration from fish gills, we have successfully constructed a self-breathing zinc-air battery (ZAB) that can extract dissolved oxygen from water and convert it to electrical energy. By utilizing a Co2Fe-NC catalyst and a large-window cathode, the ZAB demonstrates a continuous discharge duration of 68.6 h in the underwater condition, thanks to its well-engineered architecture, optimized catalyst, and gas diffusion layer. Meanwhile, the underwater ZAB outperforms fish gills as it also operates efficiently in air, exhibiting a high capacity of 740 mA h g–1 and a high power density of 320 mW cm–2. Additionally, prolonged cycling tests indicate the ZAB’s capability for stable charging and discharging with minimal potential variation. This study introduces an innovative approach to the development of breathable underwater metal–air batteries.
研究领域:
材料科学
·
能源技术
·
仿生学
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DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Nataliya A. Gavrilenko,Tatyana N. Volgina,Evgeniy V. Pugachev,Mikhail A. Gavrilenko
Polymethylmethacrylate matrix has been developed as an analytical device for visual and spectrophotometric determination of malachite green. Based on the studied effects of different pH levels, temperature, contact time and diverse ions, optimum analysis conditions have been established. For malachite green, the calibration graph was linear in the range of 0.5–30 mg·L −1 in the samples with the detection limit of 1 mg·kg −1 . The proposed simple method makes it possible to determine malachite green visually in the concentration range 8–30 mg·L −1 using a colour scale. The method has been implemented for determination of malachite green in various sea fish samples.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2023
·Vol. 42
·期 8
·867-872
·
IF 5.4
Yurong Zhang,Lijun Li,Ruteng Wang,Junwei Liu,Zhen Lin,Gang Li,Hongtao Liu,Yifeng Lei,Yunhe Xiong,Yan Zhao,Longjian Xue
Comprehensive Summary In nature, fishes have evolved functional skins with effective hydrodynamic performance and anti-fouling, facilitating predation and escaping from predators. Although a large number of fish scale-inspired structured surfaces have been explored, the incorporation of mucus on the structured surfaces has been largely ignored. Inspired by the skin of Osteichthyes fishes, a Janus hydrogel coating (JHC) is successfully prepared by a two-step UV light irradiation at room temperature. The bottom side of JHC (STH) achieves a shear adhesive strength of 103.3 ± 17.5 kPa and can strongly adhere to a large variety of surfaces, including metals, ceramic and polymers. The top surface of JHC (SLH) replicates the structure of cycloid scales, while the nature of hydrogel mimics the mucus on fish skin. SLH possesses prominent mechanical, anti-swelling, anti-fouling and drag reduction properties. The design strategy for JHC has potential applications in numerous fields, like, pipeline transportation, bioengineering, and shipping industry.
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高影响因子
ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Meng Yang,Wen-Xiong Wang
Although nanoplastics are being increasingly scrutinized, little is known about their kinetic behavior in living organisms, especially in cellular systems. Herein, nonspecific interactions of three polystyrene nanoplastics (pristine-PS, NH 2 -PS, and COOH-PS, with size range of 90–100 nm and at concentrations of 0–100 μg mL −1 ) with zebrafish cells were quantified for their cellular uptake and exocytosis. Cell uptake of nanoplastics reached a peak within 2 h and then decreased. The overall nanoplastics uptake was dominated by PS-particle internalization. The estimated uptake rate was comparable among the different types of PS (pristine-PS, NH 2 -PS, and COOH-PS), but the uptake capacity was related to their functionality. The clathrin-mediated and caveolae-mediated pathways were mainly involved in the uptake of the three nanoplastics. The internalized PS-particles were initially delivered to the cytoplasm but then transported to lysosomes using energy. Meanwhile, these PS particles were released by the cells via energy-free penetration and energy-dependent lysosomal exocytosis. PS-particles were removed by the cells at a relatively slow rate, and the estimated retention half-lives of these PS-particles were 10.1 h, 12.0 h and 15.1 h for pristine-PS, NH 2 -PS and COOH-PS particles, respectively, in fish cells based on our kinetic measurements. Intracellular trajectory modeling of nanoplastics movement is critical for the environmental and human health risk assessment.
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DOI
PubMed
高影响因子
ANIMAL FEED SCIENCE AND TECHNOLOGY
·
2022
·
IF 3.313
Shiwei Xie,Dan Wei,Yongjian Liu,Lixia Tian,Jin Niu
Dietary fish meal (FM) and fish oil (FO) replacement are crucial for the sustainable development of aquaculture. An eight weeks feeding trial was conducted to evaluate the potential replacing FM with soy protein concentrate , and the suitable FO level for a low FM diet of Penaeus monodon . A diet containing 25% FM was used as the control (C diet), and three other diets were formulated to contain 15% FM, and supplemented with 3%, 2%, 1% FO [high FO (HF), medium FO (MF) and low FO (LF), respectively]. The experiment was conducted in quadruplicates (30 shrimp per replicate, average weight 1.00 ± 0.01 g). The results indicated that besides the final body weight, there were no differences in weight gain, feed efficiency and survival rate observed among the four groups. The results of the acute salinity stress showed that shrimp fed the C and MF diets showed a similar survival rate, which was significantly higher than those fed a LF diet. Shrimp that were fed HF, MF and LF diets showed a lower level of fatty acid transport-related mRNA expression in the hepatopancreas , compared with those fed the C diet. Shrimp fed HF, MF and LF diets showed lower expression levels of superoxide dismutase , endoplasmic reticulum protein 57 and immune deficiency (IMD) in the hepatopancreas than those fed the C diet. Shrimp that were fed a LF diet showed the lowest expression levels of inhibitor of apoptosis proteins, Toll and tumor necrosis factor receptor associated factor 6 (TRAF6) in the hepatopancreas, as well as the highest expression levels of Toll, extracellular signal-regulated kinase, Relish and TRAF6 in the intestine among four groups. Intestinal microstructure damage was observed in shrimp that were fed a LF diet, and intestinal endoplasmic reticulum (ER) swelling was observed in shrimp fed MF and LF diets. In conclusion, these results indicated that the growth performance of shrimp was not affected when the FM and FO contents decreased, whereas a low content of FM negatively influenced the immune response in shrimp by modulating IMD and ER stress related gene expression. Under low FM conditions, a suitable dietary FO level would benefit to the lipid transport, hepatopancreas and intestine health, as well as the immune response of shrimp.
研究领域:
水产养殖
·
营养学
·
分子生物学
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DOI
高影响因子
Materials
·
2020
·Vol. 13
·期 19
·4370
·
IF 3.057
Ling Pan,Peng Li,Yubo Tao
As a natural macromolecule-based biomaterial, fish gelatin is used in medical materials for its low pathogen infection risk. However, because of poor mechanical properties, its application has been limited. In this study, microcrystalline cellulose-reinforced fish gelatin (FG/MCC) composite films were prepared with a biological cross-linking agent (genipin) under ultrasonic treatment. SEM micrographs showed that the smooth microstructure of FG film became increasingly disordered with the addition of MCC. The infrared spectrum analysis (FTIR) demonstrated the existence of hydrogen bond interaction between MCC and FG. Compared with the pure FG film, the tensile strength (TS) and modulus of elasticity (MOE) of composite films with MCC were improved, and the elongation at break (EAB) and swelling ratios (SR) were decreased. Ultrasonic treatment could further improve TS, MOE, and SR. When the composite film was prepared with 15% MCC and treated with ultrasound, the TS and MOE increased by 115% and 227%, respectively, while the EAB decreased by 35% and the SR decreased by 4% in comparison with pure FG films. Thermo-gravimetric analysis (TGA) showed that the FG/MCC composite films were stable below 100 °C. The above results indicate that the FG/MCC films have optimistic application prospects in the biomedical field.
研究领域:
材料科学
·
生物医学
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DOI
高影响因子
Cell Reports Methods
·
2026
·
IF 5.8
Yi-Jun Zhu,Ya-Qian Wang,Hua-Tai Xu
Mapping neuronal connectivity is essential for understanding the structure and function of neural circuits. While high-throughput, cost-effective methods using barcoded rabies viruses provide valuable cellular-level insights, they are limited by the spatial resolution of barcode sequencing. To provide a fluorescent in situ hybridization (FISH)-compatible alternative for spatial barcode readout, we developed the CASS (combination of artificial short sequences) barcode, an error-robust and in situ -hybridization-detectable tool. By combining CASS barcoding with rabies virus monosynaptic tracing, we enable in situ connectome mapping through FISH-based barcode decoding. Using this approach, we identified 1,532 synaptic pairs connected to neurons in the primary visual cortex with spatial precision across three mice, demonstrating its efficiency and scalability. This method provides a FISH-based approach for identifying neuronal connectivity with spatial context and may facilitate future integration of connectivity mapping with molecular profiling, thereby advancing our understanding of neural circuits.
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Wenhui Zhu,Wenxuan Wang,Yingnan Liu,Ying Bu,Jianrong Li,Xuepeng Li
BACKGROUND Melanoidins, as a functional component, exhibit antioxidant properties. However, the antioxidant mechanism of melanoidins in fish sauce remains poorly understood. The present study focused on the structural characteristics, microstructure and antioxidant activity of melanoidin substances in fish sauce. RESULTS Ultrafiltration and color difference analysis were utilized to confirm the main molecular weight composition of melanoidins. The ultrafiltration component > 10 kDa in fish sauce exhibited the darkest and reddest color, along with the highest content of melanoidins (699.5 mg g −1 ). The spectral characteristics were consistent with typical melanoidins. Gas chromatography–tandem mass spectrometry and Fourier transform infrared spectroscopy analyses revealed that the melanoidins contained a significant number of oxygen-containing groups, amino compounds, carbohydrates, aromatic compounds and carbonyl compounds (CO), pyrrole (C N), among others. In total, 129 alkanes, 71 esters, nine olefins and two alkynes were identified. The melanoidins (> 10 kDa component) had the strongest antioxidant activity, including a reducing power of 0.8, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity of 67.7% and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of 92.4%. Additionally, the melanoidins (> 10 kDa component) had the highest total phenolic content at 48.2 μg gallic acid equivalents mL −1 . CONCLUSION Melanoidins are important factors affecting the antioxidant activity of fish sauce. There were differences in the structural properties of melanoidin fractions with different molecular weights. © 2024 Society of Chemical Industry.
研究领域:
食品科学
·
化学
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产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Junjie Zeng,Minhui Lu,Yu Wang,Xiaozhi Zhao,Yuanjin Zhao
Microneedles are demonstrated as an effective strategy for chronic wound treatment. Great endeavors are devoted to developing microneedles with natural compositions and potent functions to promote therapeutic effects for wound healing. Herein, a novel graphene oxide-integrated methacrylated fish gelatin (GO-FGelMA) microneedle patch encapsulated with bacitracin and vascular endothelial growth factor (VEGF) is developed for chronic wound management. As the natural components and porous structures of FGelMA, the fabricated microneedle patches display satisfactory biocompatibility and drug-loading ability. Owing to the integration of graphene oxide, the microneedle patches can realize promoted drug release via near-infrared (NIR) irradiation. Besides, the encapsulated bacitracin and VEGF endow the microneedle patches with the ability to inhibit bacterial growth and promote angiogenesis. It is demonstrated that the GO-FGelMA microneedle patches with efficient drug release exert a positive influence on the wound healing process through reduced inflammation, enhanced wound closure, and improved tissue regeneration. Thus, it is believed that the proposed drugs-loaded GO-FGelMA microneedle patches will hold great potential in future chronic wound treatment.
研究领域:
生物材料学
·
伤口愈合研究
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Zhitao Chen,Yue Lin,Xiaoming Ma,Longhua Guo,Bin Qiu,Guonan Chen,Zhenyu Lin
Fish product is one of the main seafood with the disadvantage of easy to perish. Various indexes and diverse methods have been developed to evaluate the fish freshness, but most of them are complex and cannot be applied for on-field detection. In this study, we reported a simple, visual and economical multicolor biosensor based on the etching of gold nanorods (GNRs) to evaluate the fish freshness with the naked eye. Hypoxanthine (Hx) is chosen as the index of fish freshness and it react with the dissolved oxygen to produce H 2 O 2 at the presence of xanthine oxidase (XOD). Then GNRs are etched quickly by the H 2 O 2 in the presence of Fe 2+ . Correspondingly, the GNRs surface plasmon resonance (SPR) is regulated and results in a distinctly color change of the system, which can be easily distinguished with the naked eye. Therefore, the concentration of Hx in the fish samples can be semi-quantitatively analyzed with the naked eye. The proposed multicolor Hx sensor has been successfully applied to the detection of Hx in fish samples.
研究领域:
食品科学
·
生物传感技术
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）