ELECTROPHORESIS
·
2021
·Vol. 42
·期 11
·1196-1201
·
IF 3.535
Wei Wang,Huimin Zhang,Xiufeng Yu,Shaoyan Zhang
In this work, high-speed micellar electrokinetic chromatography with LIF detection was applied to study the antagonism between three intestinal bacteria, Escherichia coli ( E. coli ), Bacillus licheniformis ( B. licheniformis ) and Bacillus subtilis ( B. subtilis ). The fluorescent derivatization for the bacteria was performed by labeling the bacteria with FITC. In a high-speed capillary electrophoresis (HSCE) device, the three bacteria could be completely separated within 4 min under the separation mode MEKC. The BGE was 1 × TBE containing 30 mM SDS and 1.5 × 10 –5 g/mL polyethylene oxide. The limits of detection for E. coli , B. licheniformis and B. subtilis were 2.80 × 10 6 CFU/mL, 1.60 × 10 6 CFU/mL and 1.90 × 10 6 CFU/mL respectively. Lastly, the method was applied to investigate the antagonism between the three bacteria. The bacteria were mixed and cultured for 7 days. The samples were separated and determined every day to study the interaction between bacteria. The results showed that B. licheniformis and B. subtilis could not inhibit each other, but they could effectively inhibit the reproduction of E. coli . The method developed in this work was quick, sensitive and convenient, and it had great potential in the application of antagonism study for bacteria.
研究领域:
微生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale Advances
·
2018
·Vol. 1
·期 1
·162-167
·
IF 0
Liping Song,Lei Zhang,Kai Xu,Youju Huang,Pan Gao,Haiyan Fang,Jiawei Zhang,Zhihong Nie,Tao Chen
Current technologies still face a big challenge to achieve simultaneous rapid qualitative and quantitative detection of trypsin. In our present study, we developed a simple and effective strategy to sensitively, qualitatively and quantitatively analyze the activity of trypsin using a fluorescent polystyrene (PS) microsphere probe. PS spheres were first functionalized by the surface coating of polyethylene glycol (PEG), which could significantly decrease the possibility of nonspecific physical adsorption of the fluorescein isothiocyanate isomer-modified peptide (peptide-FITC). Then, the obtained PS-PEG spheres were chemically interacted with peptide-FITC, which were then employed to monitor the real-time activity of trypsin. The peptide used in our work contained rich lysine and arginine residues, which were the recognition sites of trypsin. When trypsin interacted with the PS-FITC-peptide microspheres, the peptide-FITC rapidly decomposed into free small fragments in solution, resulting in a gradual decrease in the fluorescence of the PS spheres. By taking advantage of the fluorescence changes using confocal microscopy imaging and fluorescence spectrum intensity, it is easy to achieve the qualitative and quantitative detection of trypsin, with a highly sensitive detection limit as low as 0.5 ng mL−1 and high selectivity. Thus, the designed fluorescent PS microsphere probe would be very promising in various applications such as food safety inspection, personal healthcare and on-site environmental monitoring.
研究领域:
生物化学
·
分析化学
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
Foods
·
2024
·Vol. 13
·期 18
·3019
·
IF 4.7
Yutong Li,Shangkun Li,Di Li,Yuan Gao,Shuhua Kong,Jingyi Liu,Shu Liu,Yichao Ma,Hui Zhou,Dandan Ren,Qiukuan Wang,Yunhai He
In this study, a quantitative method based on fluorescein isothiocyanate (FITC)-labelledHizikia fusiformepolyphenol–polysaccharide complex (HPC) and its purified fractions (PC1, PC4) was used, and its pharmacokinetics and tissue distribution were investigated in mice. The results showed that the FITC-labelled method had good linearity (R2> 0.99), intra-day and inter-day precision (RSD, %) consistently lower than 15%, recovery (93.19–106.54%), and stability (RSD < 15%), which met the basic criteria for pharmacokinetic studies. The pharmacokinetic and tissue distribution results in mice after administration showed that all three sample groups could enter the blood circulation. and HPC-FITC had a longer half-life (T1/2: 26.92 ± 0.76 h) and mean retention time (MRT0–∞: 36.48 h) due to its larger molecular weight. The three groups of samples could be absorbed by the organism in a short time (0.5 h) mainly in the stomach and intestine; the samples could be detected in the urine after 2 h of administration indicating strong renal uptake, and faecal excretion reached its maximum at 12 h. The samples were also detected in the urine after 2 h of administration. This study provides some theoretical basis for the tissue distribution pattern of polyphenol–polysaccharide complex.
研究领域:
食品科学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2025
·Vol. 150
·期 4
·750-759
·
IF 3.3
QiKe Wang,JieMiao Yu,ZhaoXiang Zhong,Cai Hui,Yi Zhang,Huizhou liu,LiangRong Yang
Sensitive and rapid detection methods for rare earth elements (REEs), including lanthanides (Lns), will facilitate the mining and recovery of these elements. Here, we innovated a rapid, highly selective and sensitive fluorescence detection method for Lns, based on Hans-Lanmodulin, a newly discovered protein with high selectivity and binding affinity for rare earth elements. By labelling the fluorescein moiety FITC onto Hans-Lanmodulin, named as FITC-Hans-LanM. When rare earth ions are present in solution, FITC-Hans-LanM will specifically bind rare earth ions undergoing a conformational change from a disordered state to a dimer, in which the FITC molecules come close to each other, resulting in decreasing fluorescence intensity or even quenching. The assay was responsive to light, medium and heavy rare earth ions. The fluorescence signal has a good linear relationship with Nd3+ concentration in the range of 1–20 nM. The detection limit of the method was 0.512 nM, within 1 min. This method could become a useful technique for the detection and quantification of rare earth elements in environmental and industrial samples.
研究领域:
生物化学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 11
·3180
·
IF 4.412
Yina Cai,Binxue Ren,Chifang Peng,Cunzheng Zhang,Xinlin Wei
New nanocomposites, Fe3O4@Au–FITC, were prepared and explored to develop a fluorescent detection of Pb2+. The Fe3O4@AuNPs–FITC nanocomposites could be etched by Pb2+in the presence of Na2S2O3, leading to fluorescence recovery of FITC quenched by Fe3O4@Au nanocomposites. With the increase of Pb2+concentration, the fluorescence recovery of Fe3O4@AuNPs–FITC increased gradually. Under optimized conditions, a detection limit of 5.2 nmol/L of Pb2+with a linear range of 0.02–2.0 µmol/L were obtained. The assay demonstrated negligible response to common metal ions. Recoveries of 98.2–106.4% were obtained when this fluorescent method was applied in detecting Pb2+spiked in a lake-water sample. The above results demonstrated the high potential of ion-induced nanomaterial etching in developing robust fluorescent assays.Keywords:gold nanoparticles;magnetic nanoparticles;FRET;nanomaterial etching;lead ions
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
He Chen,Tianyang You,Lingfeng Zong,Liliya I. Mukhametova,Dmitry O. Zherdev,Sergei A. Eremin,Yuan Ding,Minghua Wang,Xiude Hua
Peptides obtained from phage display libraries are valuable reagents for small-molecule immunoassays. However, their application in fluorescence polarization immunoassays (FPIAs) is limited by phage particles. Here, monomer, dendrimer-like dimer, tetramer peptidomimetic and anti-immunocomplex tracers were designed and synthesized using lysine as special scaffolds and spacers to develop competitive and noncompetitive FPIAs for benzothiostrobin. The affinity between tracers and monoclonal antibodies or immunocomplexes increased with the tracer valence. A higher signal-to-noise ratio and sensitivity could be generated in the FPIAs based on tetramer tracers. The sensitivities of competitive (50% inhibitory concentration) and noncompetitive (50% saturation concentration) FPIAs were 19.71 ± 4.65 and 40.43 ± 2.73 ng mL −1 , respectively. The spiked recoveries were 78.3%−105.2% with relative standard deviations (RSDs) of 0.7%−15.4% for the competitive FPIA, while 78.7%−115.3% with RSDs of 0.7%−12.5% for the noncompetitive FPIA. The amounts of benzothiostrobin in rice detected by the FPIAs were consistent with those detected by high performance liquid chromatography.
研究领域:
分析化学
·
免疫学
·
药学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 31
·15263-15274
·
IF 3.553
Wang Jie,Li Zhifeng,Yin Yamin,Liu Haisha,Tang Guohua,Ma Yanxia,Feng Xin,Mei Han,Bi Jinlian,Wang Ke,Chen Zuanguang
Abstract The construction of effective and safe theranostics was very important to realize tumor diagnosis and treatment simultaneously. Photothermal therapy had obtained great attention due to its remarkable capability of both cancer treatment and biosecurity. Molybdenum disulfide (MoS 2 ) nanoflakes have shown excellent photothermal conversion efficiency, which were explored to photothermal therapy. However, MoS 2 nanoflakes would decrease or quench the intensity of fluorescence when the MoS 2 nanoflakes directly incorporated with fluorescence dyes to construct bioimaging theranostic systems. In this work, MoS 2 nanoflakes embedded into MSNs to construct photothermal nanomaterials, which realized the nanoparticle size controllable. Fluorescein isothiocyanate (FITC) conjugated with mesoporous silica nanoparticles via amide bonds for bioimaging. It was more stable than physical absorption. As expected, the as-prepared FITC–MoS 2 MSNs possessed photothermal effect, fluorescence stability and photostability. FITC–MoS 2 MSNs were quickly taken up by HepG2 cells. Meanwhile, the HepG2 cells could be efficiently ablated under the 808 nm near-infrared laser irradiation. These results proved that we successfully constructed multifunctional MSNs for tumor theranostics. It also provided a potentially common nanoplatform for theranostic of biomedical applications. Graphic abstract
研究领域:
纳米技术
·
癌症研究
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Nano Research
·
2022
·Vol. 16
·期 4
·5383-5390
·
IF 10.269
Mao Guobin,Yang Yang,Cao Shijie,Ye Silu,Li Yifang,Zhao Wei,An Hongwei,Liu Yingxia,Dai Junbiao,Ma Yingxin
The global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has necessitated rapid, easy-to-use, and accurate diagnostic methods to monitor the virus infection. Herein, a ratiometric fluorescence enzyme-linked immunosorbent assay (ELISA) was developed using Si-fluorescein isothiocyanate nanoparticles (FITC NPs) for detecting SARS-CoV-2 nucleocapsid (N) protein. Si-FITC NPs were prepared by a one-pot hydrothermal method using 3-aminopropyl triethoxysilane (APTES)-FITC as the Si source. This method did not need post-modification and avoided the reduction in quantum yield and stability. The p-nitrophenyl (pNP) produced by the alkaline phosphatase (ALP)-mediated hydrolysis of p-nitrophenyl phosphate (pNPP) could quench Si fluorescence in Si-FITC NPs via the inner filter effect. In ELISA, an immunocomplex was formed by the recognition of capture antibody/N protein/reporter antibody. ALP-linked secondary antibody bound to the reporter antibody and induced pNPP hydrolysis to specifically quench Si fluorescence in Si-FITC NPs. The change in fluorescence intensity ratio could be used for detecting N protein, with a wide linearity range (0.01–10.0 and 50–300 ng/mL) and low detection limit (0.002 ng/mL). The concentration of spiked SARS-CoV-2 N protein could be determined accurately in human serum. Moreover, this proposed method can accurately distinguish coronavirus disease 2019 (COVID-19) and non-COVID-19 patient samples. Therefore, this simple, sensitive, and accurate method can be applied for the early diagnosis of SARS-CoV-2 virus infection.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
病毒学
·
诊断医学
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2016
·Vol. 141
·期 15
·4766-4771
·
IF 4.033
Jingni Fu,Changqin Ding,Anwei Zhu,Yang Tian
Intracellular pH plays a vital role in cell biology, including signal transduction, ion transport and homeostasis. Herein, a ratiometric fluorescent silica probe was developed to detect intracellular pH values. The pH sensitive dye fluorescein isothiocyanate isomer I (FITC), emitting green fluorescence, was hybridized with reference dye rhodamine B (RB), emitting red fluorescence, as a dual-emission fluorophore, in which RB was embedded in a silica core of ∼40 nm diameter. Moreover, to prevent fluorescence resonance energy transfer between FITC and RB, FITC was grafted onto the surface of core–shell silica colloidal particles with a shell thickness of 10–12 nm. The nanoprobe exhibited dual emission bands centered at 517 and 570 nm, under single wavelength excitation of 488 nm. RB encapsulated in silica was inert to pH change and only served as reference signals for providing built-in correction to avoid environmental effects. Moreover, FITC (λem = 517 nm) showed high selectivity toward H+ against metal ions and amino acids, leading to fluorescence variation upon pH change. Consequently, variations of the two fluorescence intensities (Fgreen/Fred) resulted in a ratiometric pH fluorescent sensor. The specific nanoprobe showed good linearity with pH variation in the range of 6.0–7.8. It can be noted that the fluorescent silica probe demonstrated good water dispersibility, high stability and low cytotoxicity. Accordingly, imaging and biosensing of pH variation was successfully achieved in HeLa cells.
研究领域:
生物化学
·
细胞生物学
·
纳米材料科学
✓ 已验证
DOI
PubMed
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2025
·
IF 3.7
Wenna Wang,Peng Zhao,Li Cai,Ting Zhao,Yingying Guo,Kexi Sun,Zuju Shu,Lijun Zhang,Zhongbo Li
Developing simple and reliable sensors for food freshness is a key goal in intelligent packaging. In this study, a ratiometric fluorescent nanofibre film was successfully developed for real-time visual monitoring of shrimp freshness. The film was constructed by embedding an amine-responsive fluorophore, fluorescein isothiocyanate (FITC) and a stable reference fluorophore, Eu (DBM) 3 Bpy, into an electrospun polyvinyl alcohol (PVA) matrix. The sensing principle relies on the pH-driven enhancement of FITC's green emission (530 nm) in the presence of biogenic amines, whereas the Eu-complex provides a constant red reference signal (610 nm). This dual-emission system creates a clear fluorescence colour change from orange to yellow-green as shrimp spoils. The sensor exhibited high selectivity and a rapid response, detecting spoilage within 6 h at 25°C. The maximum colour difference (ΔE) reached 64.78, allowing for unambiguous visual detection. This robust and easy-to-fabricate nanofibre film holds great promise for practical applications in food safety and intelligent packaging technology.
研究领域:
食品科学与技术
·
传感器与检测技术
·
智能包装
·
纳米材料
·
荧光传感
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF DRUG TARGETING
·
2024
·
IF 4.3
Yangcheng He,Yuyi Feng,Danxai Qiu,MinHua Lin,Hai Jin,Zhiwen Hu,Xue Huang,Suihong Ma,Yan He,Meiqi Lai,Wenhui Jin,Jianhua Liu
Numerous nanomedicines have been developed recently that can accumulate selectively in tumours due to the enhanced permeability and retention (EPR) effect. However, the high interstitial fluid pressure (IFP) in solid tumours limits the targeted delivery of nanomedicines. We were previously able to relieve intra-tumoural IFP by low-frequency non-focused ultrasound (LFNFU) through ultrasonic targeted microbubble destruction (UTMD), improving the targeted delivery of FITC-dextran. However, the accumulation of nanoparticles of different sizes and the optimal acoustic pressure were not evaluated. In this study, we synthesised Cy5.5-conjugated mesoporous silica nanoparticles (Cy5.5-MSNs) of different sizes using a one-pot method. The Cy5.5-MSNs exhibited excellent stability and biosafety regardless of size. MCF7 tumour-bearing mice were subjected to UTMD over a range of acoustic pressures (0.5, 0.8, 1.5 and 2.0 MPa), and injected intravenously with Cy5.5-MSNs. Blood perfusion, tumour IFP and intra-tumoural accumulation of Cy5.5-MSNs were analysed. Blood perfusion and IFP initially rose, and then declined, as acoustic pressure intensified. Furthermore, UTMD significantly enhanced the accumulation of differentially sized Cy5.5-MSNs in tumour tissues compared to that of the control group, and the increase was sevenfold higher at an acoustic pressure of 1.5 MPa. Taken together, UTMD enhanced the infiltration and accumulation of Cy5.5-MSNs of different sizes in solid tumours by reducing intra-tumour IFP.
研究领域:
肿瘤学
·
纳米医学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials
·
2026
·Vol. 19
·期 12
·2502
·
IF 3.7
Honglin Zhang,Mingkuan Xu,Ye Phone Myint,Ke Zhang,Caixia Chi,Sai Chen,Tong Zhang,Jiupeng Zhao,Yao Li
HighlightsA FRET system (FITC-PtOEP) was integrated into a photonic crystal film for oxygen sensing.Imidazole modification boosted fluorescence intensity by 52.4% under 100% O2atmosphere.Imidazole also provided excellent photostability with a 99.77% fluorescence retention rate.Fluorescence resonance energy transfer (FRET) was implemented in a photonic crystal-structured oxygen sensing film by incorporating fluorescein isothiocyanate (FITC) with platinum octaethylporphyrin (PtOEP). The highest Stern-Volmer quenching constant (KSV= 20.20 ± 0.25) and maximum quenching ratio (21.81 ± 0.21) were achieved at a PtOEP:FITC molar ratio of 1:1. Compared to the control, theKSVand maximum quenching ratio increased by 56.8% and 48.4%, respectively. Additionally, imidazole was introduced into the oxygen-sensing film via a casting method. The results demonstrated that imidazole effectively modulates the energy transfer efficiency between oxygen molecules and PtOEP. Under 20% O2and 100% O2atmospheres, the fluorescence intensity of the imidazole-modified film increased by 18.4% and 52.4%, respectively. Furthermore, imidazole provided excellent protection for PtOEP, yielding a fluorescence retention rate of 99.77% ± 0.18%.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）