NEW JOURNAL OF CHEMISTRY
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2019
·Vol. 43
·期 37
·14763-14771
·
IF 3.069
Yu Bai,Ming-Xia Wu,Qiu-Juan Ma,Chun-Yan Wang,Jing-Guo Sun,Mei-Ju Tian,Jian-Sheng Li
Cysteine, as an important amino acid in the human body, plays a vital role in people's normal life activities. In this paper, a ratiometric fluorescent probe for detecting cysteine was designed and synthesized based on the fluorescence resonance energy transfer (FRET) process. In this FRET system, a coumarin derivative was used as the energy donor, a rhodol fluorophore was chosen as the energy receptor, and an acrylate group was utilized as a cysteine recognition unit. In the absence of cysteine, the rhodol receptor was in the non-fluorescent lactone state and the FRET process was inhibited. Upon addition of cysteine, the closed spirolactone form was converted to a conjugated fluorescent xanthene form to induce the occurrence of FRET which resulted in a fluorescent signal decrease at 470 nm and enhancement at 543 nm. The ratiometric fluorescent probe exhibited excellent selectivity to Cys over Hcy and GSH. In addition, I543nm/I470nm of the probe for cysteine displayed a good linear relationship in the range of 5.0 × 10−7–1.0 × 10−4 mol L−1, and the detection limit was 2.0 × 10−7 mol L−1. Furthermore, the probe showed low cell toxicity and was successfully applied to the confocal imaging of cysteine in HepG2 cells using dual emission channels.
研究领域:
生物化学
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分析化学
·
医学影像
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Weiliang Shen,Hanhan Xu,Jing Feng,Wei Sun,Guoxing Hu,Yonghong Hu,Wenge Yang
Based on the principle of FRET, we have developed a ratiometric and colorimetric fluorescent probe TFBN, which can specifically recognize SO 2 derivatives (SO 3 2− /HSO 3 − ), and exhibit a transition from red to green fluorescence under 405 nm excitation. The probe TFBN owns the advantages of short response time (<3 min), quantitative detection SO 2 derivatives in two linear ranges, extremely low detection limit (39 nM), large Stokes shift (239 nm) and wide emission window gap (140 nm). In addition, the NBC structure was used as a fluorescent donor for FRET probes for the first time, which expanded the diversity of donors. Importantly, with low toxicity and good biocompatibility, the probe TFBN successfully detects exogenous and endogenous sulfites in living cells. These characteristics endow the probe TFBN can be successfully used in living cells and mouse imaging.
研究领域:
生物化学
·
分析化学
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医学影像学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 34
·7133-7139
·
IF 4.543
Yike Fu,Tong Chen,Gang Wang,Tongxu Gu,Congkun Xie,Jie Huang,Xiang Li,Serena Best,Gaorong Han
MicroRNAs (miRNAs) play a key role in regulating gene expression but can be associated with abnormalities linked to carcinogenesis and tumor progression. Hence there is increasing interest in developing methods to detect these non-coding RNA molecules in the human circulation system. Here, a novel FRET miRNA-195 targeting biosensor, based on silica nanofibers incorporated with rare earth-doped calcium fluoride particles (CaF2:Yb,Ho@SiO2) and gold nanoparticles (AuNPs), is reported. The formation of a sandwich structure, as a result of co-hybridization of the target miRNA which is captured by oligonucleotides conjugated at the surface of CaF2:Yb,Ho@SiO2 fibers and AuNPs, brings the nanofibers and AuNPs in close proximity and triggers the FRET effect. The intensity ratio of green to red emission, I541/I650, was found to decrease linearly upon increasing the concentration of the target miRNA and this can be utilized as a standard curve for quantitative determination of miRNA concentration. This assay offers a simple and convenient method for miRNA quantification, with the potential for rapid and early clinical diagnosis of diseases such as breast cancer.
研究领域:
生物传感器
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纳米技术
·
分子生物学
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癌症研究
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Zongwen Tang,Xing Liu,Benchao Su,Qi Chen,Hongmei Cao,Yonghuan Yun,Yang Xu,Bruce D. Hammock
Ochratoxin A (OTA) is a major concern for public health and the rapid detection of trace OTA in food is always a challenge. To minimize OTA exposure to consumers, a nanobody (Nb)-mediated förster resonance energy transfer (FRET)-based immunosensor using quantum dots (Nb-FRET immunosensor) was proposed for ultrasensitive, single-step and competitive detection of OTA in agro-products at present work. QDs of two sizes were covalently labeled with OTA and Nb, acting as the energy donor and acceptor, respectively. The free OTA competed with the donor to bind to acceptor, thus the FRET efficiency increased with the decrease of OTA concentration. The single-step assay could be finished in 5 min with a limit of detection of 5 pg/mL, which was attributed to the small size of Nb for shortening the effective FRET distance and improving the FRET efficiency. The Nb-FRET immunosensor exhibited high selectivity for OTA. Moreover, acceptable accuracy and precision were obtained in the analysis of cereals and confirmed by the liquid chromatography-tandem mass spectrometry. Thus the developed Nb-FRET immunosensor was demonstrated to be an efficient tool for ultrasensitive and rapid detection of OTA in cereals and provides a detection model for other toxic small molecules in food and environment.
研究领域:
食品安全
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生物技术
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分析化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Haixia Zhang,Cuiyan Tong,Jie Sha,Bingxin Liu,Changli Lü
This paper reported a facile method for fabricating a functionalized fluorescent graphene oxide (GO) by covalently grafting the amine-modified mesoporous silica nanoparticles (MSNs) containing poly(p-phenylenevinylene) (PPV) and lactam of Rhodamine 6G (SRh6G) inside the channel of MSNs. The resulted GO-PPV@MSN-NH 2 @SRh6G fluorescent hybrid nanoparticles had a good dispersion and strong green fluorescence in aqueous solution. This hybrid material can be used as a fluorescent ratiometric probe to detect Hg 2+ in the water through fluorescence resonance energy transfer (FRET). Upon the addition of only Hg 2+ ions, the mission corresponding to the ring-opened form of rhodamine appeared at 550 nm with a simultaneous emission quenching of PPV at 494 nm. This ratiometric fluorescence change indicated that an FRET from the PPV to rhodamine was triggered by Hg 2+ ions. The hybrid nanoparticles of GO-PPV@MSN-NH 2 @SRh6G could be used as a novel fluorescent probe to carry out the quantitative detection of Hg 2+ with a detection limit of 7.1 × 10 −8 M.
研究领域:
化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2022
·Vol. 32
·期 3
·1099-1107
·
IF 2.525
Wang Kun,Dong Erfei,Fang Min,Zhu Weiju,Li Cun
A new hybrid fluorescent nanosensor (Flu@Mea-CdS) for the Cu 2+ detection in aqueous solution was constructed through fluorescence resonance energy transfer (FRET). The Flu@Mea-CdS was fabricated by amide linkage between CdS quantum dots capped with cysteamine (Mea-CdS) and fluorescein. With the formation of FRET process from Mea-CdS quantum dots to fluorescein, the fluorescence intensity of fluorescein at 520 nm was significantly enhanced. In addition, the sensor based on FRET has high selectivity for Cu 2+ ions detection. With the presence of Cu 2+ ions, Cu 2+ ions were transferred to Cu 2 S by the reaction with Flu@Mea-CdS, which caused the inhibition of FRET process and quenched the fluorescence signal of 520 nm. Compared with Mea-CdS quantum dots, the Flu@Mea-CdS sensor has a lower detection limit for Cu 2+ . The linear range is 4–14 μM, and the detection limit is 0.17 μM. The sensor has been successfully applied to the detection of Cu 2+ ions in practical samples, which shows its potential application value in environmental monitoring.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-5
·
IF 5.228
Wu Wen-Li,Wang Zhao-Yang,Dai Xi,Miao Jun-Ying,Zhao Bao-Xiang
Bisulfite plays crucial roles in diverse physiological processes. Therefore, the efficient detection of bisulfite is very important. In this study, we report a colorimetric and ratiometric fluorescent probe (CPT) with a large Stokes shift (162 nm) for bisulfite (HSO 3 − ) based FRET mechanism. The probe can quantitatively detect HSO 3 − with low detection limit (45 nM) and high specificity over other common anions and biothiols. A nucleophilic addition reaction was proposed for the sensing mechanism, which was confirmed by HRMS spectra. The test strips of the probe were made and used easily. Moreover, probe CPT was used to ratiometric fluorescent imaging of exogenous and endogenous HSO 3 − in living cells.
研究领域:
生物化学
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分析化学
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医学检测
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Ruiqing Feng,Lifang Guo,Jinglong Fang,Yue Jia,Xueying Wang,Qin Wei,Xiaoqiang Yu
Mitochondria membrane potential (MMP) play significant roles during metabolism, signaling, and other important bioevents. Visualization of MMP levels is essential for many biological researches. However, fluorescent probes for monitoring MMP levels in dual emission colors are still deficient, which greatly limited the development of relative research areas. In this work, a pair of fluorescent probes have been designed and synthesized to monitor the MMP levels in dual emission colors based on Forster resonance energy transfer (FRET) mechanism. The FRET donor (FixD) is constructed by linking a benzyl chloride group to a fluorophore with bright-green emission. The FixD could target mitochondria and be immobilized in mitochondria by linking to the thiol group of mitochondrial proteins. The FRET acceptor (LA) is designed with green absorption and deep-red emission. In live cells with high MMP levels, FixD and LA both target mitochondria, and deep-red (DR) emission could be detected with the excitation of 405 nm. Particularly, the spectral shift of fluorescence upon the decrease of MMP is up to 110 nm, which is greatly favorable for the clear observation of MMP levels. With the decrease of MMP, LA would be released from mitochondria while FixD would still be immobilized in mitochondria, and decreased DR emission and increased green fluorescence could be detected due to the absence of FRET. In this manner, the MMP levels could be monitored in dual emission colors.
研究领域:
生物化学
·
分子生物学
·
细胞生物学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Wenhui Hao,Gengli Huang,Guoyong Jiang,Sa-adu Abiola Dauda,Fuwei Pi
To control and on-site evaluate the contamination of organophosphorus pesticides, one direct sensing approach based on spontaneous fluorescence resonance energy transfer (FRET) was developed in this study. The luminescent metal-organic frameworks (LMOFs), a novel achievement of MOFs, could provide adjustable adsorption capacity and fluorescence index to scientific analysis was first employed to evaluate phoxim molecules in vegetables and fruits. Through the π-π recognition and FRET sensing between phoxim molecules and skeleton of Nu-1000 LMOFs probes, an ultra-low detection limit of 1.65 pg/L in the range of 5×10 −7 to 5×10 −3 mg/mL ( R 2 =0.998) was achieved in the presence of phoxim. Such simple and accurate LMOFs- FRET-based sensing strategy could offer valuable reference for developing sensitive FRET sensors and/or on-site evaluation approaches in the areas of food quality control and food safety.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Tianzi Li,Zhenguang Wang,Dafeng Jiang,Henggang Wang,Wing-Fu Lai,Yunkai Lv,Yongqing Zhai
A Förster resonance energy transfer (FRET)-based biosensor using Cu nanoclusters (NCs) as energy donors and signal magnifiers and MnO 2 nanospheres as both energy acceptors and target recognizers for selective detection of glutathione (GSH) was developed. MnO 2 nanospheres, synthesized through an ultrasonication assisted top down method, can quench the photoluminescence (PL) of Cu NCs through FRET, after forming complex through electrostatic interactions. GSH can recover PL of Cu NCs by triggering the decomposition of complex through digesting MnO 2 nanospheres into Mn 2+ . Additionally, the PL can be further magnified through the host-guest interactions between GSH and Cu NCs, with around 25 times higher than the original intensity and 3 times higher than the initial PL intensity of Cu NCs. Under the optimum conditions, the FRET based sensor can detect GSH in the concentration range from 0 to 140 μM, with a detection limit of 17 μM. Moreover, the proposed method shows a strong anti-interference ability, achieving the sensitive directly detection of GSH in human whole blood samples. Our results provide insights in designing FRET based sensors. Moreover, all the components are nontoxic and biodegradable, demonstrating their potential applications in other fields such as nanomedicine and environmental science.
研究领域:
生物传感
·
分析化学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
Materials Chemistry Frontiers
·
2021
·Vol. 5
·期 23
·8229-8237
·
IF 6.482
Junjun Wang,Yize Zhang,Zhi-Peng Yu,Houshi Huang,Lei Chen,Xiaojiao Zhu,Jie Zhang,Lianke Wang,Jianhua Yu,Hongping Zhou
Phototheranostics, the integration of phototherapy and multimodal imaging into one nanoagent, is highly significant for precise cancer treatment, during which how the photo-energy is converted plays a critical role. The Förster resonance energy transfer (FRET) mechanism has opened up a new avenue for diversifying energy conversion, demonstrating great promise for enhanced phototheranostics. Herein, a theranostic nanoagent (HCY–TPA), based on single-molecule FRET, was designed, where the harvested photo-energy was not only transformed into near-infrared fluorescence but also acoustic energy for deep-seated tumor imaging, as well as enhanced thermal and type-I ROS generation for efficient treatment, realizing optimized phototheranostics. Furthermore, the dual cation endowed it with superior mitochondrial targeting ability for precise treatment. In vivo mouse model experiments demonstrated that the proliferation of tumors was effectively inhibited, compared to that by single HCY or TPA, which ultimately demonstrated a synergistic effect in multimodal imaging guiding synergistic cancer treatment.
研究领域:
生物医学工程
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纳米技术
·
癌症研究
·
分子成像
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2020
·
IF 1.467
Xinyue Zhao,Xiaomeng Zhang,Mingwei Qin,Yuzhu Song,Jinyang Zhang,Xueshan Xia,Xiuming Cui,Kai Gao,Qinqin Han
Because of diverse adverse effects of carbendazim (CBZ) upon the human body and environment, a simple method for the determination of this molecule is urgently needed. Therefore, a method based on an aptamer was developed. An aptamer of carbendazim, CZ12, labeled with a fluorescent molecule (FAM), was adsorbed on graphene oxide (GO) by π-π stacking. The fluorescence resonance energy transfer (FRET) between the molecule and GO was used to rapidly quench the fluorescence signal. In the presence of carbendazim, the fluorescent molecule selectively combines with carbendazim, thereby greatly reducing the binding efficiency of the former with GO. Thus, by mixing a FAM-labeled carbendazim aptamer, GO, and food samples, the carbendazim content was determined from the fluorescence measurements. The limit of quantification was 10.0 ng/mL for strawberry, banana, pitaya, and Panax notoginseng samples. The linear range was from 0 to 125 ng/mL with a linear correlation coefficient equal to 0.9918.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）