JOURNAL OF ALLOYS AND COMPOUNDS
·
2021
·
IF 5.316
Hong Chen,Yan Lei,Xiaogang Yang,Chaoliang Zhao,Zhi Zheng
The recombination of photoinduced charge carriers is detrimental to the photovoltaic performance of solar cells. In this work, we used CdS as an under-layer to reduce the recombination of photoinduced charge carriers at the Ag 2 S/FTO (F-doped SnO 2 ) interface. The CdS under-layer was deposited onto an FTO substrate using a facile chemical bath deposition (CBD) method. By controlling the thickness of the CdS film between Ag 2 S and FTO, the recombination of photoinduced charge carriers was successfully suppressed. The effect of the CdS under-layer on the charge carrier recombination was carefully evaluated by transient surface photovoltage (TSPV) measurements, electrochemical impedance spectroscopy (EIS) and a photoelectrochemical method. The power conversion efficiency (PCE) of solar cell devices with a 40 nm CdS under-layer is 1.16%, which is ~52% enhancement compared to those of FTO/Ag 2 S-based solar cell devices. Our work may provide a useful strategy to suppress photoinduced charge carrier recombination at the electrode and absorber semiconductor interface.
研究领域:
材料科学
·
光伏技术
✓ 已验证
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Limei Sun,Ming Gao,Qiuhui Qian,Ziyi Guo,Peng Zhu,Xuedong Wang,Huili Wang
Chronic exposure of triclosan (TCS) to zebrafish triggers high incidence of fatty liver and hepatitis; however, the underlying molecular mechanisms remain unclear. Herein, we identified miR-30b as a sensitive biomarker to TCS stress, reflecting in that its decreased expression caused metabolic toxicity, abnormal development and behavior, and lipid-metabolism disorder. By microinjecting the inhibitor and mimic experiments, miR-30b was proved to regulate lipid metabolism by its main target gene fto . Over-expression of FTO resulted in fat accumulation, elevation of the TG and TC levels and up-regulation of the PPARγ and CEBPα, as well as decrease of the global m 6 A level in larvae. On the contrary, the knock-down of FTO using MO caused the anti-lipogenic effect, decrease of the TG and T-CHO levels, and abnormal changes of cebpɑ , acsl 5, fasn , ppap 2c and ppar γ etc. Further fortification tests of cycloleucine and betaine evidenced that the toxic effect was strongly dependent on regulation of the m 6 A level. The toxicity effects in the treatments of methylated donors and receptors were consistent with the changes in physiological functions of FTO knockdown and overexpression. The effects of cycloleucine on m 6 A level and lipid metabolism generally consisted with those of FTO, but this was not the case for betaine, reflecting in increased m 6 A level and lipid accumulation in larval liver. Consequently, we posit that TCS exposure caused zebrafish lipid-metabolism disorder by decreasing miR-30b expression to regulate fto-mediated m 6 A methylation level. These findings contribute to our deep understanding of the underlying molecular mechanisms regarding contaminant-originating fatty liver and hepatocellular carcinoma, and also have practical significance in pollution warning and target therapy for related diseases.
研究领域:
毒理学
·
分子生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Huahui Gao,Xinmei Song,Qirong Chen,Ruo Yuan,Yun Xiang
The demethylase of fat mass and obesity related protein (FTO) is critical to regulate the dynamic N 6 -methyladenosine (m6A) modification of eukaryotic mRNAs, and its overexpression has found to be closely related to the initiation of several cancers. On the basis of a target-promoted specific activation of DNAzyme strategy coupled with self-primer exponential amplification reaction (SPEXPAR) cycles and DNA supersandwich assemblies, the highly sensitive and label-free electrochemical FTO assay approach is established. The modification of the catalytic core nucleobase of the DNAzyme probe by m6A can inhibit its cleavage activity. The presence of target FTO catalyzes the elimination of the methyl group to restore the DNAzyme activity, which cleaves the hairpin substrates to trigger the SPEXPAR for yielding many ssDNAs. The capture of these DNAs on the sensor electrode leads to the initiation of supersandwich assembly formation of long dsDNAs . Tremendous electrochemical signal probe of [Ru(NH 3 ) 6 ]Cl 3 are then absorbed on these dsDNAs to produce highly amplified catalytic currents with the assistance of K 3 [Fe(CN) 6 ] for detecting trace FTO with 63.1 fM detection limit. Furthermore, the sensor can be employed for selective assay of FTO in cell lysates, revealing the great potential of this sensing strategy for biomedical and biological study applications.
研究领域:
生物化学
·
分子生物学
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分析化学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2014
·
IF 4.086
Huanmei Ma,Jianhua Tian,Shuming Bai,Xiaodong Liu,Zhongqiang Shan
In this paper, Pt-Ru/Ni-P/FTO has been designed and fabricated as the counter electrode for dye-sensitized solar cells. The Pt-Ru catalytic layer and Ni-P alloy sublayer are prepared by traditional electrodeposition method and a simple electroless plating method, respectively, and the preparation conditions have been optimized. The scanning electron microscopy (SEM) images show that the Pt-Ru particles are evenly distributed on FTO and Ni-P/FTO substrate. By X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), it is confirmed that the Ni-P amorphous alloy has been formed, and no other compounds involved Ni and P have been formed. The electrochemical measurement results reveal that the Pt-Ru electrode has higher catalytic activity and stability towards tri-iodine reduction reaction than Pt electrode in the organic medium. The Ni-P sublayer deposited on FTO glasses increases the conductivity and light-reflection ability of the counter electrode, and this contributes to lowering the inner resistance of the cell and improving the light utilization efficiency. Through the photovoltaic test, it is confirmed that the energy conversion efficiency of a single DSSC with the optimized Pt-Ru/Ni-P/FTO counter electrode is increased by 29% compared with that of the cell based on the Pt/FTO counter electrode under the same conditions.
研究领域:
材料科学
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Lixiao Zhou,Renjie Li,Fu Wang,Ruiqi Zhou,Yinyin Xia,Xuejun Jiang,Shuqun Cheng,Fanghong Wang,Danyang Li,Jun Zhang,Lejiao Mao,Xuemei Cai,Hongyang Zhang,Jingfu Qiu,Xin Tian,Zhen Zou,Chengzhi Chen
Excessive exposure to metals in daily life has been proposed as an environmental risk factor for neurological disorders. Oxidative stress is an inevitable stage involved in the neurotoxic effects induced by metals, nevertheless, the underlying mechanisms are still unclear. In this study, we used arsenic as a representative environmental heavy metal to induce neuronal oxidative stress and demonstrated that both in vitro and in vivo exposure to arsenic significantly increased the level of N6-methyladenosine (m6A) by down-regulating its demethylase FTO. Importantly, the results obtained from FTO transgenic mice and FTO overexpressed/knockout cells indicated that FTO likely regulated neuronal oxidative stress by modulating activating transcription factor 3 (ATF3) in a m 6 A-dependent manner. We also identified the specific m6A reader protein, YTHDC1, which interacted with ATF3 and thereby affecting its regulatory effects on oxidative stress. To further explore potential intervention strategies, cerebral metabolomics was conducted and we newly identified myo-inositol as a metabolite that exhibited potential in protecting against arsenic-induced oxidative stress and cognitive dysfunction. Overall, these findings provide new insights into the importance of the FTO-ATF3 signaling axis in neuronal oxidative stress from an m 6 A perspective, and highlight a beneficial metabolite that can counteract the oxidative stress induced by arsenic.
研究领域:
神经科学
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毒理学
·
分子生物学
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代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS LETTERS
·
2017
·
IF 2.572
Yang Ren,Xiaoge Zhou,Gaoyang Zhao
The selection of Low-e coated glass in the mid-latitude region has been a difficult subject. In this paper, a simple sol–gel method can be used to control the grain size of fluorine-doped tin oxide (FTO) nanocrystalline films by introducing complex chelating agents into the sols and controlling their contents. The film is a polycrystalline film with rutile structure. Through the electrochemical doping technology, the localized surface plasmonic electrochromism of FTO nanocrystals in the near infrared region (NIR) can be achieved, and the optical contrast at 1750 nm can reach 60%. The Low-e glass coated with this film can adjust dynamically the transmittance of NIR light according to people’s feelings, realize the effective control of the solar radiation heat through windows without affecting the daylight use at work time, and is suitable for using in mid-latitude zone.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS
·
2024
·Vol. 24
·期 12
·3809
·
IF 3.4
Feng Chen,Mingfu Zhao,Bin Zhang,Minggang Zhao,Ye Ma
Copper ion (Cu2+) pollution poses a serious threat to marine ecology and fisheries. However, the complexity of seawater and its interference factors make the online detection of Cu2+quite challenging. To address this issue, we introduce the concept of the photo-assisted adjustment barrier effect into electrochemical detection, using it as a driving force to generate electrochemical responses. The Schottky barrier demonstrates a remarkable regulatory influence on the electrochemical response under photoexcitation, facilitating the response through Cu2+adsorption. We developed a 4-MBA-AuNPs/CdS/FTO composite that serves as a sensitive platform for Cu2+detection, achieving a detection limit of 70 nM. Notably, the photo-assisted adjustment of the barrier effect effectively counters the interference posed by ions in seawater, ensuring accurate detection. Furthermore, the sensor exhibits a promising recovery rate (99.62–104.9%) in real seawater samples, highlighting its practical applications. This innovative approach utilizing the photo-assisted adjustment barrier effect offers a promising path for developing electrochemical sensors that can withstand interference.
研究领域:
环境科学
·
分析化学
·
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Gut Microbes
·
2025
·
IF 11
Ruonan Chen,Xiaoqin Chai,Yunxiao Zhang,Tianxiu Zhou,Yinyin Xia,Xuejun Jiang,Bo Lv,Jun Zhang,Lixiao Zhou,Xin Tian,Ruonan Wang,Lejiao Mao,Feng Zhao,Hongyang Zhang,Jun Hu,Jingfu Qiu,Zhen Zou,Chengzhi Chen
Fat mass and obesity-associated protein (FTO) is the key demethylase that reverses the abnormally altered N6-methyladenosine (m6A) modification in eukaryotic cells under environmental pollutants exposure. Arsenic is an environmental metalloid and can cause severe symptoms in human mainly through drinking water. However, there is no specific treatment for its toxic effects due to the uncovered mechanisms. We previously revealed that exposure to arsenic increased the level of m6A via down-regulation of FTO, which might serve as a potential target for intervention against arsenic-related disorders. In this study, our results demonstrated that chronic exposure to arsenic significantly disrupted the intestinal barrier and microenvironment. Also, this administration resulted in the enhancement of m6A modification and the reduction of FTO expression in the intestine. By using both CRISPR/Cas9-based FTO knock-in strategy and adeno-associated virus (AAV)-mediated overexpression of FTO in the intestine, we established for the first time that up-regulation of FTO remarkably ameliorated arsenic-induced disruption of intestinal barriers and altered microenvironment of mice. We also firstly identified a dominant gut microbial species, Desulfovibrio fairfieldensis, which was sharply reduced in arsenic-exposed mice, was able to proceed arsenic-induced neurobehavioral impairments by declining the levels of its major metabolite hydrogen sulfide. Administration of Desulfovibrio fairfieldensis could significantly alleviate the neurotoxicity of arsenic. Intriguingly, the beneficial effects of FTO against arsenic neurotoxicity possibly occurred through a novel gut–brain communication via Desulfovibrio fairfieldensis and its produced hydrogen sulfide. Collectively, these findings will provide new ideas for understanding the mechanisms of arsenic-induced toxic effects from a gut–brain communication perspective, and will assist the development of explicit intervention strategy via regulation of a new potential target FTO for prevention and treatment against arsenic-related both intestinal and neurological disorders.
研究领域:
毒理学
·
微生物学
·
神经科学
·
分子生物学
·
胃肠病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2018
·
IF 3.296
Xinxin Han,Ning Wang,Junya Li,Ying Wang,Ruiyong Wang,Junbiao Chang
Nafamostat mesilate is a serine protease inhibitor and used for the treatment of pancreatitis and cancers. The fat mass and obesity-associated protein (FTO) was identified to be a demethylase and played an important role in physiological processes. The aim of this study was to examine the inhibition of Nafamostat mesilate on FTO demethylase activity. A combination of thermodynamic and enzymatic activity studies provides an insight into the recognition of Nafamostat mesilate by FTO. The binding of Nafamostat mesilate to FTO was driven by higher positive entropy changes and smaller negative enthalpy changes. The structural and thermodynamic information provides the basis for the design of more effective inhibitors for FTO. Our results might provide a novel target protein for Nafamostat mesilate.
研究领域:
生物化学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Molecular & Cellular Toxicology
·
2025
·
IF 1.4
He Chaojie,Fan Hongyan,Zhu Chunyan,Huang Yueqing
Background Ischemia–reperfusion (I/R) injury is a severe disorder during revascularization therapy against acute myocardial infarction. Objective Our study aims to investigate the role and regulation mechanism of Annexin A1 (ANXA1) against I/R injury. Results Western blotting analysis showed that ANXA1 expression in rat cardiomyocytes (H9C2 cells) was notably decreased by oxygen–glucose deprivation/reperfusion (OGD/R) treatment. Meanwhile, the NLRP3 inflammasome activation accompanied by pyroptosis and inflammation was observed in H9c2 cells under OGD/R induction. Besides, overexpression or knockdown of ANXA1 dramatically inhibited or aggravated NLRP3-mediated pyroptosis and inflammation. Further investigations found that the m 6 A level of ANXA1 was upregulated by OGD/R, thus leading to decreased mRNA stability in H9C2 cells. FTO, a N6-methyladenosine (m 6 A) “eraser”, was downregulated in H9C2 cells by OGD/R and shown to increase the ANXA1 protein expression by demethylating mRNA in H9c2 cells under OGD/R induction. Moreover, knockdown FTO reversed the protection of ANXA1 against NLRP3-mediated pyroptosis and inflammation in OGD/R-induced H9C2 cells. Conclusion ANXA1 protects cardiomyocytes against I/R injury by alleviating NLRP3-mediated pyroptosis and inflammation via FTO-mediate demethylation. These findings innovatively suggest the therapeutic potential of ANXA1 for myocardial I/R injury.
研究领域:
分子生物学
·
心脏病学
·
表观遗传学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Cuicui Zhou,Shiqi Hong,Xinyu Chen,Quanfeng Li,Zongqi Chen,Songwang Yang
The electron transport layer (ETL) plays a critical role in efficient and stable perovskite solar cells (PSCs). The current effective method for the large-scale preparation of metal oxide ETLs is mainly based on expensive sputtering processes. Here, a screen-assisted self-spreading method is proposed as a novel approach to prepare uniformly thin and conformal TiO2 films on a rough fluorine-doped tin oxide (FTO) substrate as an ETL in planar PSCs. The TiO2 ETL deposited by this method exhibited good coverage and homogeneity on the rough FTO substrate, thereby minimizing interfacial recombination. The photovoltaic performance of the PSCs fabricated by this method is superior to that of the cells fabricated by spin coating, especially in terms of the fill factor. The performance enhancement can be attributed to the complete coverage of the FTO substrate by the conformal TiO2 film, confirming the effectiveness and reliability of the proposed method for the preparation of the TiO2 ETL. The advantages of this method lie in its scalability to prepare oxide films with a large area, eliminating the requirement of complex equipment, such as spinners, sputters, or physical vapor deposition equipment.
研究领域:
材料科学
·
能源技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2017
·
IF 4.798
Rong Wang,Shengxia Zhang,Yun Su,Junxia Liu,Ye Ying,Feng Wang,Xiaowei Cao
A semi-solid state electrochromic device with deep eutectic solvent gel as the electrolyte, Ethyl-viologen as the electrochromic compound, and K 4 Fe(CN) 6 as the electron donor was reported. Four kinds of silica were simply mixed with ethaline for preparing the gel electrolyte, and the hydrophilic ones were found to be capable of being used as the gelling agent . To enhance the electron transfer at the electrode/gel interface and keep the transparency of the electrode, tiny amount of Au was electrochemically deposited on the FTO electrode. The as-prepared electrochromic device shows a coloration time of ca. 60 s at −0.7 V with the coloration efficiency of 91 cm 2 /C, and a bleaching time of ca. 150 s at 0 V with the efficiency of 194 cm 2 /C. The reversible response of the electrochromic device up to 820 cycles with Δ T max > 60% was also demonstrated.
研究领域:
材料科学
·
电化学
·
能源存储与转换
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）