BIODEGRADATION
·
2018
·Vol. 29
·期 6
·593-603
·
IF 2.41
Shi Zhoukun,Dong Weiliang,Xin Fengxue,Liu Jiawei,Zhou Xinhai,Xu Fanli,Lv Ziyao,Ma Jiangfeng,Zhang Wenming,Fang Yan,Jiang Min
Abstract An acetamiprid-degrading fungus was isolated from contaminated soil and identified as Fusarium sp. strain CS-3 based on physiological, biochemical, and molecular analyses. Strain CS-3 exploited 50 mg/L as the sole carbon source in liquid culture, removing 98% in 96 h. Strain CS-3 retained its acetamiprid degradation abilities over a wide range of pH (5.0–8.0) and temperature (20–42 °C). HPLC–MS analysis showed that N ′-[(6-chloropyridin-3-yl)methyl]- N -methylacetamide, 2-chloro-5-hydroxymethylpyridine, and 6-chloronicotinic acid were identified as the most predominant metabolites, forming the basis for a newly described acetamiprid degradation pathway. Strain CS-3 efficiently degraded 99.6% of 50 mg/kg acetamiprid in soil, indicating potential for soil remediation. Graphical abstract
研究领域:
环境科学
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微生物学
·
生物修复
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产品关联
DOI
PubMed
高影响因子
BRAZILIAN JOURNAL OF MICROBIOLOGY
·
2022
·Vol. 54
·期 1
·199-211
·
IF 2.214
Chen Guoqiang,Xu Zhenxiang,Wang Fan,Liu Lijuan,Wei Yuxi,Li Jinglong,Zhang Liang,Zheng Kai,Wu Lijun,Men Xiao,Zhang Haibo
The exploration of polysaccharides from microorganisms is of great importance. In this study, a new type of exopolysaccharide excreted by Fusarium merismoides A6 (FM-EPS) was isolated, and the extraction conditions were optimized using a response surface methodology (RSM). The extraction temperature at 0 °C, a precipitation time of 7.83 h, and an ethanol precipitation concentration of 77.64% were predicted and proved to be the best extraction conditions with the maximum extraction yield of 0.74 g/mL. Then, two fractions of F . merismoides A6 exopolysaccharides (FM-EPS1 and FM-EPS2) were obtained through DEAE Sepharose fast flow column chromatography. As indicated by monosaccharide composition analysis, both fractions mainly consisted of mannose, glucose, galactose, and ribose, with an average molecular weight of 5.14 × 10 4 and 6.50 × 10 4 g/mol, respectively. FT-IR and NMR spectroscopy indicated the FM-EPSs had both α- and β-glycosidic bonds. Moreover, the determination of antioxidant and antiproliferative activities in vitro proved that FM-EPSs had good antioxidant activities and antiproliferation activities. FM-EPS1 showed stronger antioxidant activities than FM-EPS2. FM-EPS2 showed antiproliferation activities on HeLa and HepG2 cells, while FM-EPS1 had no obvious antiproliferative activity. Therefore, FM-EPSs could be explored as potential antioxidant and anticancer agent applied in food, feed, nutraceutical, pharmaceutical, cosmetics, and chemical industries.
研究领域:
微生物学
·
生物化学
·
药学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2016
·
IF 4.165
Li Taotao,Jian Qijie,Chen Feng,Wang Yong,Gong Liang,Duan Xuewu,Yang Bao,Jiang Yueming
Fusarium proliferatum as a common fungus pathogen in foods can produce toxic fumonisins, which can cause animal diseases and increase risks of human cancers. On contrary, butylated hydroxyanisole (BHA) as a synthetic antioxidant offers a clue for preventing growth of fungal species and inhibiting production of mycotoxins. Unfortunately, information of the inhibitory mechanism of BHA on Fusarium species is still limited. In this study, influence of BHA treatment on growth and inhibition of fumonisin production in relation to the expression of the fumonisin biosynthesis-related genes of the F. proliferatum ZYF was investigated, which revealed that BHA had a negative influence on growth and fumonisin production of F. proliferatum. To further elucidate the mechanism of BHA on the growth of F. proliferatum, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine the F. proliferatum hyphae. The BHA treatment induced the loss of cytoplasm and cellular constituents, as well as distortion of mycelia, but it did not directly degrade the fumonisin. Furthermore, the BHA treatment markedly inhibited the expressions of FUM1 (a polyketide synthase encoding gene) and FUM8 (an aminotransferase encoding gene) genes, which resulted in the depression of metabolic pathway of F. proliferatum. The transcriptional analyses of the FUM1 and FUM8 genes confirmed a correlation between the fumonisin production and its gene expression. This study provided some insights into mechanisms of production of fumonisin and feasible prevention to reduce fumonisin contamination in favor of human and animal health.
研究领域:
微生物学
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真菌毒素研究
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食品安全
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Bin Bin Huang,Ming Wei Gao,Guo Li,Ming-An Ouyang,Qi-Jian Chen
In research related to fungicides, the development of compounds from natural products with high antifungal activity has attracted considerable attention. Fusaric acid (FA), an alkaloid isolated from the metabolites of Fusarium oxysporum, is an important precursor for developing pharmacologically active herbicides. In our previous work, we reported that FA has a wide range of inhibitory activities against 14 plant pathogenic fungi. In particular, it exhibited excellent antifugal effects on Colletotrichum higginsianum (EC50 = 31.7 μg/mL). Herein, to explore the practical application in the agricultural field, the design and synthesis of three series of FA derivatives and their inhibitory activities against plant pathogenic fungi were examined. Results demonstrated that the optimized FA derivatives had excellent inhibitory activities against C. higginsianum, Helminthosporium (Harpophora maydis), and Pyricularia grisea. In particular, the inhibitory activities were considerably improved when the 5-butyl groups of FA were substituted. The EC50 of C. higginsianum and P. grisea was only 1.2 and 12.0 μg/mL when 5-butylalkyl groups were substituted with 5-([1,1′-biphenyl]-4-yl) and 5-phenyl, respectively. Moreover, the safety index of target compounds, which was obtained from the treatment index of medicines, on rice seeds was evaluated. Finally, 16 leading compounds (H4, H22–H24, H27, H29, H30–H34, H37, H45, H50, H52, and H53) were obtained; they had considerable potential for additional modification and optimization as agricultural fungicides. Moreover, three-dimensional quantitative structure–activity relationship models were developed for obtaining a systematic structure–activity relationship profile to explore the possibility of more potent FA derivatives as novel fungicides.
研究领域:
农药学
·
有机化学
·
植物病理学
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DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2024
·
IF 4.1
Yang Wenjuan,Tang Sidi,Xu Rubing,Zhang Lu,Zhou Zihao,Yang Yong,Li Yanyan,Xiang Haibo
Fusarium is a soil-borne pathogen that poses a serious threat to the quality and yield of hundreds of crops worldwide, particularly tobacco production. Using metabolomics technology, we investigated natural metabolites from disease-conducting soil (DCS) and disease-suppressing soil (DSS) of tobacco rhizosphere as fungicides to control tobacco Fusarium wilt (TFW), which is mainly caused by Fusarium oxysporum. Furthermore, the antifungal mechanisms of these natural metabolites were preliminarily elucidated through various assessments, including antifungal activity determination, chemotaxis effect tests, PI staining experiments, and measurements of extracellular conductivity and protein content. Metabolomics results showed that the DCS with three different disease grades (G1, G5 and G9 groups) had significantly higher levels of 15, 14 and 233 differential rhizosphere metabolites (DRMs) and significantly lower levels of 72, 152 and 170 DRMs compared to the DSS (G0 group). According to KEGG pathway analysis, these DRMs were found to be enriched in the caffeine metabolism, biosynthesis of phenylpropanoids, galactose metabolism and tyrosine metabolism, etc. Linustatin, scopoletin and phenylpropiolic acid were picked out from these DRMs and found to have suppressive activity against F. oxysporum through correlation analysis and antifungal experiments. The three DRMs showed strong inhibitory effects on the growth and spore germination of F. oxysporum at concentrations of 0.5 mM or higher in each test period. Furthermore, F. oxysporum showed a phobotaxis effect against these three DRMs at concentrations as low as 0.25 mM. Finally, we found that the three DRMs had an inhibitory effect on F. oxysporum by destroying the integrity of the cell membrane and increasing the membrane permeability of F. oxysporum. This study firstly reports the inhibition activity of phenylpropiolic acid and linustatin on F. oxysporum, providing a practical and environmentally friendly method for biocontrol of TFW by using natural fungicides.
研究领域:
植物病理学
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代谢组学
·
农业科学
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真菌学
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产品关联
DOI
PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2024
·
IF 6
Linlin Su,Wenyun Li,Xiaohua Chen,Pengcheng Wang,Diqiu Liu
The root rot mainly caused by Fusarium solani is a bottleneck in the cultivation of Panax notoginseng . In this study, we reported a gene encoding a plant cell wall structural protein, P. notoginseng proline-rich protein (PnPRPL1), whose transcription was upregulated by F. solani and induced by some hormone signals. The PnPRPL1 recombinant protein significantly inhibited the growth and conidial germination of the root rot pathogens. Downregulation of PnPRPL1 by RNA interference (RNAi) in P. notoginseng leaves increased the susceptibility to F. solani , whereas overexpression of PnPRPL1 in tobacco ( Nicotiana tabacum ) enhanced the resistance to F. solani . Compared with wild-type tobacco, the PnPRPL1 -overexpressing transgenic tobacco had higher reactive oxygen species (ROS)-scavenging enzyme activities, lower ROS levels, and more lignin and callose deposition. The opposite results were obtained for the P. notoginseng expressing PnPRPL1 RNAi fragments. Furthermore, the PnPRPL1 promoter transcription activity was induced by several plant hormones and multiple stress stimuli. In addition, the transcription factor PnWRKY27 activated the expression of PnPRPL1 by directly binding to the promoter region. Thus, PnPRPL1 , which is positively regulated by a WRKY transcription factor, encodes an antimicrobial protein that also mediates ROS homoeostasis and callose/lignin deposition during the response to F. solani infection.
研究领域:
植物病理学
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分子生物学
·
植物遗传学
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DOI
PubMed
高影响因子
BIOLOGICAL CONTROL
·
2014
·
IF 1.873
Waseem Raza,Jun Yuan,Ning Ling,Qiwei Huang,Qirong Shen
The volatile organic compounds (VOCs) produced by antagonistic microbes have great antifungal potential against soil-borne fungal pathogens. The VOCs produced by Paenibacillus polymyxa strain WR-2 in the presence of root exudates and organic fertilizer were identified and their effects on the growth and spore germination of Fusarium oxysporum f. sp. niveum were evaluated. The VOCs produced by WR-2 inhibited the growth of F. oxysporum by 38%, 36% and 40% in agar medium, sterilized soil and natural soil, respectively. This inhibitory effect was increased to 60%, 58% and 64% with the addition of organic fertilizer in agar medium, sterilized soil and natural soil, respectively. The addition of root exudates did not affect the production of antifungal VOCs by WR-2. The VOCs produced by WR-2 completely inhibited the germination of F. oxysporum spores. Out of 42 identified VOCs, seven VOCs; benzothiazole, benzaldehyde, undecanal, dodecanal, hexadecanal, 2-tridecanone and phenol were found to inhibit the growth of F. oxysporum . The results of these experiments suggest another significance of using organic fertilizer as a carrier material with the biocontrol agents to control soil-borne fungal pathogens.
研究领域:
微生物学
·
植物病理学
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DOI
高影响因子
JOURNAL OF PHYTOPATHOLOGY
·
2024
·Vol. 172
·期 2
·e13301
·
IF 1.1
Guoyu Wei,Wei Zhao,Huayang Xu,Guangming Wang,Hualei Wang,Anlong Hu
Polygonatum cyrtonema (giant Chinese Solomon's seal) is a perennial herb belonging to the family Liliaceae, with significant medicinal and dietary value. However, a severe rhizome rot has led to a decline in the yield and quality of this species. In this study, a fungus was isolated from symptomatic rhizome samples and identified as Fusarium commune through molecular and morphological analyses. In pathogenicity tests, the identification of F. commune as the causative agent of rhizome rot was confirmed by fulfilling Koch's postulates. Biological characteristics indicated that the optimal growth conditions for F. commune were a temperature of 25°C, pH of 8, carbon source of soluble starch, nitrogen source of peptone and a photoperiod of 24 h light. In order to reduce the use of chemical fungicides and explore natural substances to control the disease, the sensitivity of F. commune to seven phytochemicals was studied. Four phytochemicals (honokiol, 2-allylphenol, magnolol and carvacrol) showed inhibitory activity, with honokiol having a 50% of the maximal effect concentration (EC 50 ) of 9.99 ± 0.14 mg/L. The results of pot experiment showed that the four phytochemicals had significant control efficacy on the rhizome rot of P. cyrtonema , and the highest control efficacy was observed with honokiol. These findings provide a scientific basis for the control of rhizome rot in P. cyrtonema .
研究领域:
植物病理学
·
植物化学
·
药用植物
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产品关联
DOI
高影响因子
JOURNAL OF PLANT PATHOLOGY
·
2021
·Vol. 103
·期 2
·549-561
·
IF 1.729
Win Theint Theint,Bo Bo,Malec Przemyslaw,Khan Sikandar,Fu Pengcheng
Trichoderma sp. was isolated from the rhizosphere soil of a healthy banana plant grown in a Panama disease-contaminated field (suppressive soil). The selected strain, identified as Trichoderma asperellum , exhibited antifungal activity against four species of phytopathogenic fungi from the Fusarium genus ( F. oxysporum, F. fujikuroi, F. tricinctum, F. cantenulatum ). Antagonism related mechanisms associated with the T. asperellum isolate against four fungal phytopathogens were studied in vitro both indirectly by the observation of growth competition and directly by antibiosis and mycoparasitism . It was shown that T. asperellum could suppress growth of phytopathogens in the range of 65–74%, as well as inhibits spore germination by 30- 75%. Moreover, T. asperellum was demonstrated to secrete mycolytic enzymes (chitinase and β-1,3, glucanase), which are potentially able to degrade cell walls of the phytopathogens. The observed accumulation of respective transcripts and enzymatic activities of both chitinase and β-1,3, glucanase were remarkably higher in pathogen-induced cultures. The reduction of disease severity by 70% was observed in pot cultures of banana plantlets infected previously with F. oxysporum after 9 weeks of treatment with T. asperellum. Our findings demonstrate that T. asperellum as originated from banana wilt-contaminated plantations express anti-pathogenic effects based on various modes of action, including competitiveness, antibiosis, and mycoparasitism and might be potentially used as a biological control agent to cope with diseases caused by a broad spectrum of pathogenic fungi of Fusarium genus.
研究领域:
植物病理学
·
微生物学
·
生物防治
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Tian-Qiong Shi,Yi-hang Shen,Ya-Wen Li,Zi-Yi Huang,Zhi-Kui Nie,Chao Ye,Yue-Tong Wang,Qi Guo
Gibberellic acid (GA3), produced industrially by Fusarium fujikuroi , stands as a crucial plant growth regulator extensively employed in the agriculture filed while limited understanding of the global metabolic network hinders researchers from conducting rapid targeted modifications. In this study, a small-molecule compounds-based targeting technology was developed to increase GA3 production. Firstly, various small molecules were used to target key nodes of different pathways and the result displayed that supplement of terbinafine improved significantly GA3 accumulation, which reached to 1.08 g/L. Subsequently, lipid and squalene biosynthesis pathway were identified as the key pathways influencing GA3 biosynthesis by transcriptomic analysis. Thus, the strategies including in vivo metabolic engineering modification and in vitro supplementation of lipid substrates were adopted, both contributed to an enhanced GA3 yield. Finally, the engineered strain demonstrated the ability to achieve a GA3 yield of 3.24 g/L in 5 L bioreactor when utilizing WCO as carbon source and feed.
研究领域:
植物科学
·
分子生物学
·
生物技术
·
代谢工程
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产品关联
DOI
PubMed
高影响因子
Microorganisms
·
2023
·Vol. 11
·期 6
·1431
·
IF 4.5
Guoyu Wei,Wei Zhao,Anlong Hu,Mingjian Ren,Yunxiao Huang,Huayang Xu
Sorghum bicoloris cultivated worldwide. Leaf spot of sorghum, which leads to leaf lesions and yield reduction, is a prevalent and serious disease in Guizhou Province, southwest China. In August 2021, new leaf spot symptoms were observed on sorghum leaves. In this study, traditional methods and modern molecular biology techniques were used to isolate and identify the pathogen. Sorghum inoculated with the isolate GY1021 resulted in reddish brown lesion that similar to symptoms observed in the field: the original isolate inoculated was reisolated and Koch’s postulates were fulfilled. Based on morphological features and phylogenetic analysis of the internal transcribed spacer (ITS) combined sequence with β-tubulin (TUB2) and translation elongation factor 1-α (TEF-1α) genes, the isolate was identified asFusarium thapsinum(Strain accession: GY 1021; GenBank Accession: ITS (ON882046),TEF-1α (OP096445), and β-TUB(OP096446)). Then, we studied the bioactivity of various natural products and microorganisms againstF. thapsinumusing the dual culture experiment. Carvacrol, 2-allylphenol, honokiol, and cinnamaldehyde showed excellent antifungal activity, with EC50values of 24.19, 7.18, 46.18, and 52.81 µg/mL, respectively. The bioactivity of six antagonistic bacteria was measured using a dual culture experiment and the mycelial growth rate method.Paenibacillus polymyxa,Bacillus amyloliquefaciensandBacillus velezensisdisplayed significant antifungal effects againstF. thapsinum. This study provides a theoretical basis for the green control of leaf spot of sorghum.Keywords:antagonistic bacteria;natural product;leaf spot;sorghum;F. thapsinum
研究领域:
植物病理学
·
微生物学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Ruo-Fei Bai,Yi-Wei Wang,Shan-Shan Yang,Yu-Hao Zhang,Bo-Hang Zhou,Cong-Wei Zhou,Le Zhou
As part of our continuing research on propiolic acid derivatives, a series of benzyl propiolate derivatives and analogues were designed, synthesized, and investigated for inhibition activity in vitro and in vivo on phytopathogenic fungi, structure–activity relationship (SAR) and action mechanism. The results showed that most of the compounds had potent and broad-spectrum antifungal activity in vitro at 50 μg/mL. Among the compounds, 2-bromobenzyl propiolate (3h) displayed the highest comprehensive activity with total activity index (TAI) of 4.57 against all the eight test fungi followed by 3-fluorobenzyl propiolate (3c) (TAI = 4.53), superior to positive fungicides thiabendazole (TAI < 4.12) and/or azoxystrobin (TAI < 2.90). Relative to the other fungi, Fusarium solani and Fusarium graminearum revealed higher total susceptibility indexes (TSI) of 11.1 and 8.98, respectively, for all compounds. 3h and 3g (4-chlorobenzyl propiolate) gave the smallest EC50 values of 0.86 and 0.67 μg/mL against F. solani and F. graminearum, respectively, superior or comparable with thiabendazole and azoxystrobin. As a representative, 3h at 200 μg/mL displayed 100% protection on F. solani infections on potatoes over 7 days and high safety for plant growth. In antifungal mechanism, 3h was able to change mycelial morphology, destroy the structures of both hypha and cell membrane, increase the intracellular ROS level, decrease the intracellular mitochondrial membrane potential level, and change the permeability of the cell membrane. The SAR showed that ethynyl is a key group for the activity, and the presence of halogen atoms at the 2-site of the benzene ring can significantly improve the activity in most cases. Thus, benzyl propiolate can be considered a novel antifungal lead compound, while 3h can be considered a promising fungicide candidate to develop new agricultural fungicides.
研究领域:
农业化学
·
植物病理学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
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