ACS Sensors
·
2021
·
IF 7.711
Zongbao Sun,Yunlong Gao,Zeng Niu,Haodong Pan,Xuechao Xu,Wen Zhang,Xiaobo Zou
Paper-based fluorescence devices, with smartphone aids, bring considerable operation convenience for tetracycline (TC) sensing. Nevertheless, they must meet the challenge in real determination against complicated backgrounds. Considering that, we present a programmable-printing paper-based device and then apply it to TC determination for various natural samples. MoS2 NPs and Gmp/Eu-Cit are synthetized as composite probes. A static quenching process is found with MoS2 NP fluorescence at 430 nm, while significant magnification of Gmp/Eu-Cit emission is obtained at 617 nm, establishing a valuable ratiometric indicator. Remarkably, two-stage programmable printing maximizes the proposed sensing capability. A transitive device, containing a gradually changing amount of a certain probe, is prepared to sense TC. With a homemade smartphone application and 3D-printed measurement chamber, the corresponding signals are examined to explore optimal setups. These setups are automatically processed to prepare the final-version device, not requiring manual operations. Benefitting from this interesting feature, the proposed device gains many rewards in performances. It effectively senses TC in a wide range from 12.7 nM to 80 μM and simultaneously provides naked eye-legible signals and smartphone-based readouts with confident selectivity and stability. This device is consequently applied for various samples of soil, river water, milk, and serum and meets well with HPLC–MS and recovery tests.
研究领域:
分析化学
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环境科学
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生物医学工程
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DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Hui Huang,Meini Li,Mingwei Hao,Liangli (Lucy) Yu,Yongxin Li
A selective and sensitive colorimetric strategy for sulfide analysis was developed using GMP-Cu nanozymes with a laccase-like activity. This research discovered for the first time that sulfide could significantly enhance the catalytic activity of the GMP-Cu nanozymes by about 3.5 folds. The enhanced laccase activities duo to two reasons. First, Cu 2+ in GMP-Cu nanozymes was reduced to Cu + . The other reason was the formation of Cu–S bond which was beneficial to accelerate the electron transfer rate to improve catalytic activity. Therefore, this method showed an excellent selectivity for sulfide. And it had a linear relationship in the sulfide concentration range of 0–220 μmol/L with a detection limit of 0.67 μmol/L. Furthermore, the proposed method was successfully applied to examine sulfide in the food systems. This new method may be used in sulfide detection to improve food quality and safety.
研究领域:
分析化学
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食品科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY LETTERS
·
2021
·Vol. 43
·期 3
·677-690
·
IF 2.461
Zhang Chaolei,Wang Chao,Zhao Shan,Xiu Zhilong
Objectives Probiotics ( Bacillus subtilis 04178) were entrapped in alginate-chitosan microcapsules by high-voltage electrostatic process. The encapsulation pattern was established as entrapped low density cells with culture (ELDC wc ). The performance of ELDC wc cells was investigated against stress environments of simulated digestive fluids. Results After incubation in simulated gastric (pH 2.5) and intestinal fluids (4% bile salt) for 2 h, the survival rate of ELDC wc cells (18.19% and 27.54%) was significantly higher than that of the free cells (0.0000009% and 0.0005%). The reason why B. subtilis embedded in microcapsules can resist the stress environments was that the mass production of extracellular proteins and polysaccharides prompted B. subtilis to form cell aggregates. The production of extracellular proteins and polysaccharides were regulated by the concentration of c-di-GMP and the expression of ydaJKLMN operon, abbA , sinI , slrA , slrB , abrR and sinR . Conclusions c-di-GMP is important for the production of extracellular polymer substance to enhance probiotic viability in stress environments.
研究领域:
微生物学
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生物技术
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食品科学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2015
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IF 5.33
Xiaolei Zhang,Jingjing Deng,Yumeng Xue,Guoyue Shi,Tianshu Zhou
In this study, we demonstrate a colorimetric and fluorescent dual-mode method for alkaline phosphatase activity (APA) sensing in freshwater lake with stimuli-responsive gold nanoparticles@terbium-guanosine monophosphate (Au-NPs@GMP-Tb) core–shell nanoparticles. Initially, the core–shell nanoparticles were fabricated based on Au-NPs decorated with a fluorescent GMP-Tb shell. Upon being excited at 290 nm, the as-formed Au-NPs@GMP-Tb core–shell nanoparticles emit green fluorescence, and the decorated GMP-Tb shell causes the aggregation of Au-NPs. However, the addition of ALP destroys GMP-Tb shell, resulting in the release of Au-NPs from the shell into the solvent. As a consequence, the aggregated Au-NPs solubilizes with the changes in the UV–vis spectrum of the dispersion, and in the meantime, the fluorescence of GMP-Tb shell turns off, which constitutes a new mechanism for colorimetric and fluorescent dual-mode sensing of APA. With the method developed here, we could monitor the dynamic change of APA during an algal bloom of a freshwater lake, both by the naked eye and further confirmed by fluorometric determination. This study not only offers a new method for on-site visible detection of APA but also provides a strategy for dual-mode sensing mechanisms by the rational design of the excellent optical properties of Au-NPs and the adaptive inclusion properties of the luminescent infinite coordination polymers.
研究领域:
环境科学
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分析化学
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纳米技术
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DOI
PubMed
高影响因子
Frontiers in Immunology
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2023
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IF 7.3
Xi Zhang,Tiaoxia Wang,Xiaona Zhu,Yong Lu,Mingpeng Li,Zhihong Huang,Deping Han,Longzhen Zhang,Yang Wu,Liantao Li,Frank Klawonn,Renata Stripecke
Introduction Epstein-Barr virus (EBV) is a widely spread pathogen associated with lymphoproliferative diseases, B/ T/ NK cell lymphomas, nasopharyngeal carcinoma (NPC) and gastric carcinoma (GC). EBV lytic reactivations contribute to the genomic instability, inflammation and tumorigenesis of NPC, promoting cancer progression. Patients with NPC refractory to standard therapies show dismal survival. EBV gp350 is an envelope protein detectable in NPC specimens intracellularly and on the cell membrane of malignant cells, and is a potential viral antigen for T cell-directed immunotherapies. The potency of T cells engineered with a chimeric antigen receptor (CAR) targeting gp350 against EBV + lymphoproliferative disease was previously shown. Methods Here, we advanced towards preclinical and non-clinical developments of this virus-specific CAR-T cell immunotherapy against NPC. Different gp350CAR designs were inserted into a lentiviral vector (LV) backbone. Results A construct expressing the scFv 7A1-anti−gp350 incorporating the CD8 transmembrane and CD28.CD3ζ signaling domain (ZT002) was selected. High titer ZT002 (~1x10 8 TU/ml) was manufactured in HEK 293T/17 suspension cells in serum free media as large-scale production under good manufacturing practices (GMP). A LV multiplicity of infection (MOI) of 1 resulted in high frequencies of functional gp350CAR + T cells (>70%) at a low (<2) vector copy numbers in the genome. ZT002 was therefore used to establish gp350CAR-T batch run production methods. GMP upscaling and validation of T cell transduction and expansion in several runs resulted in average 3x10 9 gp350CAR-T cells per batch. >80% CD3 + gp350CAR-T cells bound to purified gp350 protein. In vitro cytotoxicity and cytokine secretion assays (IFN-γ and TNF-α) confirmed the specificity of gp350CAR-T cells against gp350 + NPC, GC and lymphoma cell targets. Immunocompromised B-NDG mice (NOD.CB17- PrkdcscidIl2rgtm1 /Bcgen) were challenged s.c. with a EBV + NPC C666.1 cell line expressing gp350 and then treated with escalating doses of gp350CAR-T cells or with non-transduced T cells. gp350CAR-T cells promoted antitumor responses, bio-distributed in several tissues, infiltrated in tumors and rejected gp350 + tumor cells. Discussion These results support the use of gp350CAR-T cells generated with ZT002 as an Innovative New Drug to treat patients with solid and liquid EBV-associated malignancies.
研究领域:
免疫治疗
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肿瘤学
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分子生物学
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病毒学
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临床前研究
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Yingjie Yuan,Xiangzhen Chen,Lei Jia,Yongxin Li,Dan Zhao,Lina Zhang,Yuanyuan Huang,Jun Xu,Tongqian Zhao
Long-term discharge of tetracycline (TC) in the environment exerts selective pressure on the bacteria, increasing resistance in sensitive bacteria and posing a serious threat to public health. Therefore, it is crucial to develop real-time detection of tetracycline. In this study, we reported a novel rare-earth fluorescent nanosensor FITC-GMP-Eu, based on the formation of guanosine-5′-monophosphate disodium salt (GMP) and fluorescein isothiocyanate (FITC), which enabled the real-time identification of tetracycline in the environment. When there was no TC in the environmental sample, the FITC-GMP-Eu nanosensor exhibited the green fluorescence of FITC due to the fluorescence quenching effect of water molecules on Eu 3+ . When the environmental samples contained TC, the red fluorescence of Eu 3+ could be sensitized due to the diketone structure in the tetracycline molecule, which enabled the naked-eye visualized identification of TC. The nanosensor had high sensitivity (detection limit of 19 nM) and a wide detection range (0–30 μM) for TC. At the same time, the sensor was characterized by high visualization and anti-interference ability due to the strong fluorescence of both FITC and Eu complexes. In order to improve the practicality of the detection, this study also prepared a paper-based and sodium alginate gelated fluorescence detection platform with low background fluorescence. Combined with the common color analysis and RGB extraction procedures in mobile phones, real-time and on-site identification of TC on the surface of various fruit samples can be achieved.
研究领域:
环境科学
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生物化学
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分析化学
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产品关联
DOI
高影响因子
CURRENT BIOLOGY
·
2026
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IF 7.5
Fugui Xu,Zeyu Zhang,Fengzhi Yuan,Limin Wang,Qing Yan,Mingming Yang,Xiaolong Shao,Yongxing He,Fengquan Liu,Mengcen Wang,Bingxin Wang,Guoliang Qian
Microbial competition serves as a fundamental driver for the evolution of offensive and defensive mechanisms among microorganisms. While it is well established that bacteria utilize specialized secretion systems to deliver both toxic and non-toxic effector proteins into competing cells, thereby directly killing them or modulating cellular events, it remains largely unclear whether bacteria can hijack effector proteins derived from competitors to resolve interspecies conflicts. Here, we demonstrate that Pseudomonas protegens employs a sophisticated defense strategy, hijacking LtaE, a non-cytotoxic effector delivered via the type IV secretion system (T4SS) of non-flagellated Lysobacter enzymogenes , to resolve interbacterial conflict through transcriptional reprogramming. Translocated LtaE neutralizes an uncharacterized antibacterial toxin in P. protegens . Surprisingly, as a countermeasure, P. protegens hijacks LtaE to rewire its host signaling hierarchy, converting it into a motility activation switch. Mechanistically, LtaE directly binds to FleQ, the σ54-dependent master regulator of flagellar biosynthesis, shielding it from inhibitory c-di-guanosine monophosphate (GMP) binding—a universal second messenger whose elevated concentration typically inhibits bacterial motility and promotes biofilm formation. Remarkably, the LtaE-FleQ complex remains stable under high c-di-GMP conditions, overriding sessility signals to derepress flagellar gene expression and trigger escape motility. Biochemical analyses reveal that LtaE broadly targets FleQ homologs across pseudomonads through competitive inhibition of c-di-GMP binding, linking competitor detection to motility activation. Our findings establish a novel bacterial conflict-resolution paradigm, demonstrating how non-cytotoxic effectors act as molecular switches to dynamically reprogram transcriptional networks and enhance phenotypic plasticity.
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Baoxia Liu,Haoshuang Shen,Daosheng Liu,Yuanqiang Hao,Xu Zhu,Qi Shen,Peng Qu,Maotian Xu
Metal-organic coordination polymers have emerged as attractive functional materials due to their tunable structures and unique properties. In this work, by the self-assembly of citrate (Cit) and lanthanide ions, we developed lanthanide coordination polymer nanoparticles (LCPNPs), Cit/Tb, for fluorescence sensing of guanosine-5-monophosphate (GMP). The LCPNP-based Cit/Tb sensor alone displayed weak luminescence, however, the addition of GMP to Cit/Tb led to the formation of a ternary LCPNP, Cit/Tb-GMP, with a significantly enhanced luminescence (15-fold) owing to the intramolecular energy transfer from GMP to Cit/Tb and the hydrophobic environment in the polymer interior. This luminescence turn-on assay exhibited excellent selectivity and high sensitivity for GMP with detection limit of 100 nmol L −1 . Furthermore, the Cit/Tb LCPNP possesses a long luminescence lifetime of up to a millisecond, which is important for detecting GMP in complicated samples of infant formula and ham by time-resolved fluorimetry. We believe that the presented Cit-based nanosensor would inspire the development of various new LCPNP-based fluorescent assays by utilizing Cit or other water-soluble, non-toxic organic ligands for a variety of applications in fluorescence sensing, bio-imaging, drug delivery and so on.
研究领域:
化学
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生物分析
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材料科学
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DOI
高影响因子
Food and Bioprocess Technology
·
2024
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IF 5.3
Shi Yingkun,Niu Zijun,Chen Sichun,Wang Songlei,Yang Lingfan,Wang Yihua
Abstract Electrochemical analysis is a powerful analytical technique with extensive applications in the food industry, offers high advantages due to its high sensitivity, low operating cost, and high-speed analysis. In this study, a novel nanoenzymes high-catalytic platform based on a conductive polymer (MXene) was established for the detection of Guanosine 5′-monophosphate (GMP). The MXene/Fe 3 O 4 /β-CD nanocomposites were synthesized using a self-assembly method, exhibiting significant catalytic activity and a large specific surface area. The surface morphology, functional groups and chemical bonds of MXene/Fe 3 O 4 /β-CD were analyzed via scanning electron microscopy (SEM), Fourier Transform Infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS). Electrochemical experiments indicated a significant amplification of GMP electrical signals on the nanoenzymatic platform. The oxidation of GMP to 8-oxo-GMP involved the transfer of two electrons (−2e − ) and two protons (-2H + ) at the MXene/Fe 3 O 4 /β-CD modified glassy carbon electrode. Under optimized conditions, this electrochemical nanoenzymatic platform achieved a low detection limit of 0.0126 μM for GMP, with linear ranges from 0.05 μM to 600 μM. The sensor demonstrated excellent selectivity and stability and was successfully applied for GMP determination in chicken, fish, pork and beef samples. This study provided a new pathway for the development of sensors for GMP detection. Graphical Abstract
研究领域:
食品科学
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生物传感
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材料科学
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
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2020
·
IF 0
Chun-Xia Zhang,Yan-Cai Gao,Hong-Wei Li,Yuqing Wu
Guanosine monophosphate (GMP)-protected bimetallic nanoclusters of gold and platinum, Au–PtNCs-GMP, were prepared through an improved hydrothermal method under basic conditions, which show high catalytic activity for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) under ambient conditions. Further systematic optimization of synthesis provides the best catalyst by feeding the materials 0.8 mM HAuCl4, 0.2 mM H2PtCl6, 4.0 mM NaOH, and 3.0 mM GMP, which reacted at 120 °C for 30 min, which show an initial maximum velocity (Vmax) of 253.8 × 10–8 M s–1 for TMB as the substrate. Such a value is at the top among the oxidase mimetics reported, which is essentially attributed to the synergistic effect between two metals in the small nanocluster. Moreover, the achieved Au–PtNCs-GMP show a short reaction time of 60 s for the oxidation of substrates, which is much faster than the previously reported ones. The in-depth mechanistic studies by 1H and 31P NMR spectroscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy of the optimized Au–PtNCs-GMP reveal them to be of ultrasmall size with a unique ligand structure, particularly the synergistic effect between the two metals endow the product with a high catalytic performance. Therefore, the present study not only provides a novel bimetallic nanocluster as a good catalyst with highly efficient oxidase mimetics but also opens a way for the development of other oxidase mimetics.
研究领域:
纳米生物学
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催化化学
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材料科学
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DOI
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 2
·1051
·
IF 4.9
Jiaqi Zhang,Yingying Liu,Junxing Hu,Guangxian Leng,Xining Liu,Zailin Cui,Wenzhen Wang,Yufang Ma,Shanshan Sha
Biofilm dispersal contributes to bacterial spread and disease transmission. However, its exact mechanism, especially that in the pathogenMycobacterium tuberculosis, is unclear. In this study, the cellulase activity of theM. tuberculosisRv0062 protein was characterized, and its effect on mycobacterial biofilm dispersal was analyzed by observation of the structure and components of Rv0062-treated biofilm in vitro. Meanwhile, the metabolite factors that induced cellulase-related biofilm dispersal were also explored with metabolome analysis and further validations. The results showed that Rv0062 protein had a cellulase activity with a similar optimum pH (6.0) and lower optimum temperature (30 °C) compared to the cellulases from other bacteria. It promoted mycobacterial biofilm dispersal by hydrolyzing cellulose, the main component of extracellular polymeric substrates of mycobacterial biofilm. A metabolome analysis revealed that 107 metabolites were significantly altered at different stages ofM. smegmatisbiofilm development. Among them, a decrease in gamma-aminobutyric acid (GABA) promoted cellulase-related biofilm dispersal, and this effect was realized with the down-regulation of the bacterial signal molecule c-di-GMP. All these findings suggested that cellulase promotes mycobacterial biofilm dispersal and that this process is closely associated with biofilm metabolite alterations.Keywords:biofilm;biofilm dispersal;Mycobacterium tuberculosis;cellulase;GABA;c-di-GMP
研究领域:
微生物学
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生物化学
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传染病
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2026
·
IF 5.2
Xin-Yan Zhang,Xu-Xia Yan,Shao-Yu Sun,Cheng-Zhen Wu,Hui-Dong Qiu,Qian-Yu Zhou
Laccase-like Cu-GMP complex forms directly without tedious synthesis. • The homogeneous system effectively avoids light-scattering interference. • The platform detects 2,4-DCP, BPA and epinephrine with low detection limits. • Smartphone-based colorimetry enables portable, low-cost on-site detection. • Good recoveries are achieved in lake water and human serum samples. The rapid and sensitive screening of phenolic compounds is essential for environmental safety and clinical diagnostics. Although laccase-like Cu-based nanozymes offer cost-effective alternatives to natural enzymes, most existing platforms rely on heterogeneous nanoparticles. These systems not only require tedious pre-treatment, but also suffer from light scattering that compromises colorimetric accuracy. Herein, a homogeneous colorimetric sensing platform based on the laccase-like activity of a copper-guanosine 5′-monophosphate (Cu-GMP) complex was developed. The complex is prepared directly in solution without the need for time-consuming synthesis. Furthermore, this homogeneous system eliminates light-scattering interference due to its intrinsic optical transparency. Benefiting from its catalytic versatility, the platform enabled the quantification of three phenolic targets. 2,4-dichlorophenol (2,4-DCP) and bisphenol A (BPA) were detected via 4-aminoantipyrine (4-AAP)-mediated coupling, while epinephrine was quantified by direct catalytic oxidation, with detection limits of 0.22, 0.59, and 0.21 μM, respectively. Additionally, integrating this system with smartphone technology enabled a portable platform for on-site quantification, demonstrating reliable accuracy in lake water and human serum samples. Overall, this work provides a versatile and low-cost homogeneous strategy for the point-of-need screening of phenolic pollutants and biomarkers. Download: Download high-res image (123KB) Download: Download full-size image
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DOI
高影响因子
危险品化学品经营许可证（带存储）