ACS Applied Energy Materials
·
2026
·
IF 5.5
Wenjie Gu,Lin Chen,Zejian Li,Zhiwen Xu,Yuxin Ge,Yongqi Mao,Xinyuan Song,Jiajin Li,Pengxuan Wu,Junwei Liao,Minglei Lu,Tiejun Wang
Hydrogen, with its zero-carbon nature, represents an ideal sustainable secondary energy alternative. Despite its promise for the hydrogen economy, the aqueous-phase reforming of methanol (APRM) continues to face the primary challenge of developing efficient and stable catalysts. Herein, we present a Cu-Glu/Al2O3–ZnO catalyst and investigate its structure-performance relationship through various characterization techniques. A unique core–shell structured catalyst is constructed, consisting of carbon-encapsulated nanoparticles (8.6 nm) that are evenly dispersed on an Al2O3–ZnO support. The catalyst exhibits outstanding activity at 210 °C, with a hydrogen production rate of 51.8 μmol/gcat/s, H2 selectivity of 99.95%, and CO selectivity of 0.05%. Furthermore, it demonstrates excellent durability, with no significant deactivation observed over six consecutive reaction cycles under hydrothermal conditions. In situ DRIFTS and characterization reveal that Cu species catalyze both methanol dehydrogenation (*CH3OH → *CH3O → *CH2O → *HCOOH) and water dissociation (*H2O → *H + *OH), which collectively promote efficient H2 production in APRM.
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Inorganics
·
2026
·Vol. 14
·期 3
·88
·
IF 3.4
Naifeng Chen,Yi Li,Chenxia Gao,Chao Xue,Shuang Liu,Jinghang Li,Xi Cao,Kuilin Lv,Yuguang Lv
This study systematically investigated the characterization of 2Fe-CDs/12Mn-CeO2composites and the colorimetric sensing properties of H2O2, glucose (Glu), and glutathione (GSH). The morphology, structure, and optical properties of the 2Fe-CDs/12Mn-CeO2composite were analyzed in detail by XRD, FT-IR, SEM, TEM, XPS, and Raman spectroscopy, and its formation was supported by multiple complementary characterization techniques. The catalytic efficiency (kcat/Km) of the nanozyme is 152-fold higher than natural HRP under optimal conditions and remains 59-fold higher even after temperature normalization to 25 °C. In the colorimetric sensing experiments, the detection limits of Fe-CDs/Mn-CeO2were 0.21 μM, 2.7 μM, and 0.63 μM for H2O2, Glu, and GSH, respectively. Rapid and accurate determination of the concentrations of these biomolecules can be achieved by observing the color changes after Fe-CDs/Mn-CeO2reaction with the objects to be measured. The experimental results show that Fe-CDs/Mn-CeO2have high sensitivity and selectivity for H2O2, Glu, and GSH, which provides a solid theoretical and experimental basis for the application of Fe-CDs/Mn-CeO2in the field of biosensing and medical diagnosis.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Kai Si,Tingting Gong,Suyun Ding,Huiping Liu,Shuyuan Shi,Jianqiu Tu,Linxian Zhu,Li Song,Lushan Song,Xiaowei Zhang
Phosvitin has excellent calcium binding capacity, related to its phosphopeptides. The phosphopeptides may be used as functional ingredients for improving calcium bioavailability, but the calcium-binding mechanism is unclear. In this study, a novel phosvitin phosphorylated pentapeptide (Glu-Asp-Asp-pSer-pSer, EDDpSpS) was selected to prepare an EDDpSpS calcium complex (EDDpSpS-Ca), and the calcium-binding mechanism and bioavailability investigated. The calcium-binding capacity of EDDpSpS was up to 468 ± 152.80 mg/g. Calcium ions prompted the folding of the EDDpSpS structure to form spherical nanoparticles. The calcium binding sites of EDDpSpS involved peptide bonds, carboxyl, amino, and phosphate groups. Molecular forces involved in these interactions were electrostatic in nature. Moreover, EDDpSpS-Ca had excellent bioavailability when compared to CaCO 3 , calcium lactate, and d -calcium gluconate. This study revealed the calcium-binding mechanism of phosvitin phosphopeptide, and suggested that EDDpSpS-Ca has the potential to be a novel, efficient, and promising calcium supplement.
研究领域:
营养学
·
生物化学
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PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Junhui Rong,Juan Han,Yang Zhou,Lei Wang,Chunmei Li,Yun Wang
In enzymatic conversion of biomass, how to degrade cellulose into fermentable glucose in an economic, efficient, and clean way has become an important subject. As for the application of cellulase in cellulose degradation, the process optimization in enzyme engineering is urgently desired. The traditional multistep purification processes lead to rising production costs and reduced activity of cellulase; meanwhile, the difficulty in reusability of cellulase has also become a big baffle in the cost-effective application of cellulase in biomass degradation. In this paper, the biocatalyst Glu-linker-ELP-GB (GLEGB) containing binary tags, elastin-like polypeptide (ELP), and graphene-binding (GB), was constructed to simplify the purification and immobilization of β-glucosidase (Glu) from Coptotermes formosanus. A high recovery rate (97.2%) and purification fold (18.7) of GLEGB was obtained by only one round of inverse transition cycling (ITC) with 0.5 M (NH4)2SO4 at 25 °C in a short incubating time of 10 min. The purification performance of the one-round ITC method is superior to the commonly used Ni-NTA resin affinity method. Furthermore, the high loading amounts of GLEGB immobilized on GO (698.2 mg g–1) and C3N4 (527.3 mg g–1) were achieved by the synergistic effects of ELP and GB tags. The storage stability and thermal stability of GLEGB was significantly enhanced after immobilization. The recombinant GLEGB immobilized on GO, MGO, graphite, C3N4, C200, and C400 retained 71.4%, 69.5%, 75.1%, 61.2%, 73.5%, and 80.2% of their initial activities respectively after eight cycles. It is worth mentioning that the Km values of GLEGB immobilized on lamellar carbon materials including GO, MGO, and C3N4 are very close to free GLEGB, showing a high affinity of recombinant GLEGB to substrate. To our knowledge, this is the first report on enzyme-linker-ELP-GB system with wide application prospect in the efficient purification and immobilization of enzyme, which can achieve the goal of reducing cost and improving efficiency of biocatalyst in enzymatic conversion of biomass.
研究领域:
生物技术
·
酶工程
·
生物质转化
·
材料科学
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高影响因子
MOLECULAR PHARMACEUTICS
·
2023
·
IF 4.9
Ping Cai,Sufan Tang,Li Xia,Yinwen Wang,Yang Liu,Yue Feng,Nan Liu,Yue Chen,Zhijun Zhou
Since prostate-specific membrane antigen (PSMA) is upregulated in nearly all stages of prostate cancer (PCa), PSMA can be considered a viable diagnostic biomarker and treatment target in PCa. In this study, we have developed five 68Ga-labeled PSMA-targeted tracers, 68Ga-Flu-1, 68Ga-Flu-2, 68Ga-9-Ant, 68Ga-1-Nal, and 68Ga-1-Noi, to investigate the effect of lipophilic bulky groups on the pharmacokinetics of PSMA inhibitors compared to 68Ga-PSMA-11 and then explore their in vitro and in vivo properties. 68Ga-labeled PSMA inhibitors were obtained in 88.53–99.98% radiochemical purity and at the highest specific activity of up to 20 MBq/μg. These compounds revealed a highly efficient uptake and internalization into LNCaP cells and increased over time. PET imaging and biodistribution studies were performed in mice bearing PSMA expressing LNCaP prostate cancer xenografts. All tracers enabled clear visualization of tumors in PET images with excellent tumor-to-background contrast. The biodistribution studies showed that all these radioligands were excreted mainly via the renal pathway. The in vivo biodistribution of 68Ga-Flu-1 revealed higher tumor uptake (40.11 ± 9.24 %ID/g at 2 h p.i.) compared to 68Ga-PSMA-11 (28.10 ± 5.96 %ID/g at 2 h p.i.). Both in vitro and in vivo experiments showed that chemical modification of the lysine fragment significantly impacts tumor-targeting and pharmacokinetic properties. Great potential to serve as new PET tracers for prostate cancer has been revealed with these radiotracers─68Ga-Flu-1 in particular.
研究领域:
医学影像
·
肿瘤学
·
药物化学
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Rui Su,Wei Liu,Gang Shi,Dongling Chen,Lihua Yan,Qian Li,Haichao Ni,Meili Yu,Liang Xu
Hyperbilirubinemia is primarily treated through hemoperfusion, a process that utilizes biomaterials as adsorbents to eliminate excessive bilirubin from the bloodstream. However, extracorporeal circuits are prone to thrombus formation due to the frequent use of blood-contacting devices, potentially leading to treatment failure and adverse clinical outcomes. In this study, using a resin substance treated with sulfated polysaccharides (GLU-ERM-0100), we present a reliable and safe approach for removing excessive bilirubin from blood. The resin’s pore size facilitates bilirubin adsorption, while sulfonic and hydroxyl groups incorporated within the material enable like-heparinization. GLU-ERM-0100 serves as a temporary blood-thinning agent, binding coagulation factors in the extracorporeal circuit, thereby eliminating the need for conventional anticoagulant medications. Both in vitro and animal studies demonstrate the material’s exceptional bilirubin adsorption capacity and effective anticoagulation properties.
研究领域:
医学工程
·
血液学
·
药学
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高影响因子
ChemNanoMat
·
2026
·Vol. 12
·期 6
·e70310
·
IF 2.6
Feng Feng,Ziteng Zhao,Gongrang Li,Yong Song,Qi An,Yantao Zhao
Traditional antibacterial systems are limited by poor stability of natural enzymes, weak antimicrobial activity of conventional nanozymes, and the need for exogenous H 2 O 2 or pH adjustment, thus failing to meet the demands of clinical wound care. To address these issues, this study constructed a glucose oxidase-glucose-mineral-based composite nanozyme (GLU-GOD-FAM) antibacterial system and systematically evaluated its catalytic mechanism, stability, antimicrobial activity, in vivo wound healing efficacy, and biosafety. Results showed that FAM overcame the inactivation of natural enzymes under high temperature or UV irradiation; GLU-GOD-FAM exhibited a “1 + 1 > 2” synergistic antimicrobial effect, achieving 100% bactericidal rate against E. coli and S. aureus without exogenous H 2 O 2 supplementation or pH adjustment. In vivo, it accelerated the healing of infected and noninfected mouse dorsal wounds and caused no obvious damage to major organs. This stable, efficient, and safe system provides a novel candidate for clinical emergency wound care and postoperative infection control.
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高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2026
·
IF 5.2
Eshtiag Abdalla Ibrahim Mohamed,Li-Ya Liang,Da-Jun Wu,Jia-Yi Chen,Ya-Ting Gao,Da-Wei Li,Roshila Moodley
Scandium-glutamic acid phosphorescent materials (Sc/Glu-PMs) were synthesized via a simple one-pot hydrothermal process using Sc 3+ ions and glutamic acid ligands. Structural characterization confirmed that the obtained material is an amorphous organic-inorganic composite rather than a crystalline coordination polymer. Compared with pure glutamic acid, Sc/Glu-PMs exhibited significantly enhanced room-temperature phosphorescence (RTP), attributed to coordination-induced structural rigidity that suppresses non-radiative decay pathways and stabilizes triplet excitons. Post-synthetic protonation using dilute hydrochloric acid further improved the phosphorescence quantum yield (PQY) from 7.63% to 11.07%, although a moderate decrease in phosphorescence lifetime was observed, indicating enhanced radiative decay efficiency after protonation. Surface and spectroscopic analyses suggest that protonation modifies the hydrogen-bonding environment and local surface chemistry without disrupting the bulk structure. The prepared materials also demonstrated promising optical information encryption performance, including high contrast, persistent afterglow, and good photostability under repeated UV on/off cycles. This work provides a simple coordination-protonation strategy for regulating RTP behavior in amino acid-based organic-inorganic systems for potential anti-counterfeiting and optoelectronic applications.
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高影响因子
Journal of Nanomaterials
·
2021
·Vol. 2021
·期 1
·4145010
·
IF 2.986
Jin Xu,Xuecheng Sun,Xiaodong Sun,Ye Lu
In this paper, a capillary coated with poly(glycidyl methacrylate) nanoparticles (PGMA NPs) was prepared and applied to construct a capillary electrophoresis (CE) enantioseparation system with glucosyl- β -cyclodextrin (Glu- β -CD) as a chiral selector. The PGMA NP coating can be easily introduced through a simple ring-opening reaction. Two basic drugs were used as models to evaluate the separation performance of the PGMA coating. Under the optimal conditions selected, the separation of the two enantiomers was obtained.
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高影响因子
Pharmaceutics
·
2022
·Vol. 14
·期 10
·2051
·
IF 6.525
Wei Zhou,Jiandong Huang,Qingwei Xiao,Shunmin Hu,Shijia Li,Jie Zheng,Zhiyun Du,Jiangling Peng,Huixiong Chen
The prostate specific membrane antigen (PSMA), extensively overexpressed on prostate cancer (PCa) cell surface, has been validated as a diagnostic biomarker for PCa. However, insufficient attention has been paid to the development of PSMA-specific probes loaded with small chemical molecules for the in vivo molecular imaging of PCa. In this study, we innovatively labelled superparamagnetic iron oxide nanoparticles with a PSMA-targeting Glu-Urea-Lys scaffold. An optimized synthetic route was developed to offer a physiochemically stable probe. The probe demonstrated high binding affinity (0.38 ± 0.08 μg(Fe)/mL) and binding specificity to PSMA expressed on prostate cancer cell surface in vitro. In a xenograft PCa mouse model, significant negative contrast of the implanted prostate cancer xenograft could be specifically observed by MRI 6 h after tail vein injection of the tracer (Fe, 20 mg/kg), exhibiting its potential to exclusively enhance magnetic resonance detection of PCa.Keywords:prostate cancer;PSMA;molecular imaging;MRI contrast agent;SPIONs
研究领域:
肿瘤学
·
医学成像
·
纳米技术
·
分子生物学
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-12
·
IF 5.3
Wang Yanping,Guo Min,Li Yuanyuan,Chen Yan,Wei Hong,Mo Xiaohui,Chai Guolin,Du Yongling,Hu Fangdi
A dual-template molecularly imprinted electrochemical sensor was developed for the simultaneous detection of serotonin (5-HT) and glutamate (Glu). First, amino-functionalized reduced graphene oxide (NRGO) was used as the modification material of a GCE to increase its electrical conductivity and specific surface area, using Glu and 5-HT as dual-template molecules and o-phenylenediamine (OPD) with self-polymerization ability as functional monomers. Through self-assembly and electropolymerization, dual-template molecularly imprinted polymers were formed on the electrode. After removing the templates, the specific recognition binding sites were exposed. The amount of NRGO, polymerization parameters, and elution parameters were further optimized to construct a dual-template molecularly imprinted electrochemical sensor, which can specifically recognize double-target molecules Glu and 5-HT. The differential pulse voltammetry (DPV) technique was used to achieve simultaneous detection of Glu and 5-HT based on their distinct electrochemical activities under specific conditions. The sensor showed a good linear relationship for Glu and 5-HT in the range 1 ~ 100 μM, and the detection limits were 0.067 μM and 0.047 μM ( S / N = 3), respectively. The sensor has good reproducibility, repeatability, and selectivity. It was successfully utilized to simultaneously detect Glu and 5-HT in mouse serum, offering a more dependable foundation for objectively diagnosing and early warning of depression. Additionally, the double signal sensing strategy also provides a new approach for the simultaneous detection of both electroactive and non-electroactive substances.
研究领域:
分析化学
·
神经生物学
·
医学诊断
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PubMed
高影响因子
Chinese Journal of Chromatography
·
2023
·Vol. 41
·期 2
·187-194
·
IF 0.7
Liu H,Li Y,Zi M,Chen Z,Duan A,Yuan L.
Covalent organic frameworks （COFs） are an emerging class of porous crystalline materials composed of multidentate organic units connected by covalent bonds. COFs have been demonstrated to exhibit great potential and research value in many fields， including gas storage and separation， photoelectric devices， fluorescence sensors， catalysis， drug delivery， dye and pollutant adsorption， and electronic devices， and so on. The COFs obtained by post-synthesis modification tend to exhibit high crystallinities and porosities， thereby rendering them suitable materials for use in the fields of chiral resolution， asymmetric catalysis， and chromatography. In this work， TpPa-NO 2 was synthesized from 1，3，5-tricarbaldehyde phloroglucinol and 2-nitro-1，4-phenylenediamine， which was then reduced to TpPa-NH 2 . Subsequently， this material was modified with D-glucose via a post-synthesis modification strategy to obtain the TpPa-NH 2 -Glu. TpPa-NH 2 -Glu were characterized by nuclear magnetic resonance （NMR） spectroscopy， Fourier transform-infrared （FT-IR） spectroscopy， X-ray powder diffraction （XRD） analysis， etc. In the XRD pattern， the peaks observed at 4.7°， 8.1°， 11.1°， and 27° were attributed to the TpPa-NH 2 -Glu， and these peaks are consistent with previous reports， thereby confirming the successful synthesis of this derivative. In addition， circular dichroism experiments indicated that the TpPa-NH 2 -Glu exhibited a Cotton effect， further confirming the chiral COF was prepared. Subsequently， this material was immobilized on the surface of spherical silica gel particles via the net-wrapping method to prepare a stationary phase for high performance liquid chromatographic column. Using n -hexane-isopropanol （9∶1， v/v） or methanol-water （9∶1， v/v） as mobile phases at a flow rate of 0.5 mL/min， 16 racemates and two benzene-based positional isomers （ o ， m ， p -nitroaniline and o ， m ， p -Iodoaniline） were successfully resolved by this chiral column. In addition， under methanol-water （9∶1， v/v） mobile phase conditions， five racemates were separated， among which propranolol hydrochloride， warfarin， and metoprolol reached baseline separation. Furthermore， under n -hexane-isopropanol （9∶1， v/v） mobile phase conditions， 11 racemates were resolved， among which ethyl 2-bromopropionate and 3-butyn-2-ol reached baseline separation. Meanwhile， the effect of temperature on the TpPa-NH 2 -Glu liquid chromatography column and the repeatability of the TpPa-NH 2 -Glu liquid chromatography column were also explored. The HPLC column prepared by TpPa-NH 2 -Glu had good repeatability， and its relative standard deviation （RSD） was 1.55% and 1.46%， respectively. It is demonstrated that the TpPa-NH 2 -Glu material has good resolution ability for chiral compounds.
研究领域:
分析化学
·
材料科学
·
色谱学
·
有机化学
·
分离科学
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PubMed
高影响因子
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