Journal of Functional Foods
·
2018
·
IF 3.47
Xiaona Sun,Mengmeng Duan,Yili Liu,Tengrui Luo,Na Ma,Shuang Song,Chunqing Ai
Some polysaccharides from Gracilaria lemaneiformis (GLPs) are shown to have various activities, but the effects of GLPs on diet-induced obesity and the underlying mechanisms are unclear. This study indicated that dietary supplementation with GLPs reduced weight gain and improved body composition in high fat diet (HFD)-fed mice without reduced food consumption. Fat accumulation in the liver and adipose tissues was reduced by GLPs, and the damages of HFD on the intestine were repaired. These effects could be associated with the modulation of the gut microbiota, resulting in a specific microbiota that similar to normal microbiota. Changes in the gut microbiota may affect the expression of lipid-related genes via short chain fatty acids-dependent pathways. It revealed that GLPs can alleviate HFD-induced obesity by improving the gut microbiota dysbiosis, suggesting that G. lemaneiformis can be developed as food supplement to improve metabolic syndrome and intestinal diseases.
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高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Xiao Zhang,Jude Juventus Aweya,Zong-Xun Huang,Zhuo-Ying Kang,Zi-Hao Bai,Kun-Huan Li,Xiao-Tong He,Yang Liu,Xian-Qiang Chen,Kit-Leong Cheong
The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis were examined. During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups. GLP increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes, while GLO increased the abundance of Firmicutes and Actinobacteria. Moreover, the abundances of potential pathogenic bacteria were reduced. Molecular weight and intrinsic viscosity of GLP decreased significantly from 2.15 × 10 5 to 1.22 × 10 5 Da, 374.45–113.91 mL/g, respectively. Furthermore, GLP was degraded into smaller degree of polymerization of oligosaccharides, with no significant change observed in GLO. Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.
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PubMed
高影响因子
3 Biotech
·
2017
·Vol. 8
·期 1
·1-8
·
IF 1.361
Yuan Shengliang,Duan Zhihong,Lu Yingnian,Ma Xiaoli,Wang Sheng
Gracilaria lemaneiformis is mainly used as a raw material source for agar industry, and its extract is rich in natural pigments with antioxidant activities. In this study, a solvent reflux extraction method for decolorization of G. lemaneiformis has been developed in agar production. The extraction conditions were optimized as follows: solvent-to-material ratio, 50:1; ethanol concentration, 70%; number of extractions, 3; extraction time, 0.5 h, under which the total antioxidant yield of the extract reached 2.89 ± 0.88 mg/g dried seaweeds. Their IC 50 values of DPPH radical scavenging activity, hydroxyl radical scavenging activity and superoxide anion scavenging activity were 21.91 ± 1.8 mg/L, 40.59 ± 1.5 mg/L and 160.87 ± 2.8 mg/L, respectively. Further isolation and spectroscopic analysis of natural pigments suggested the strong antioxidant capacities were attributed to chlorophyll derivatives. The results indicate that the decolorization process was able to improve the agar quality, and the extract containing lots of natural pigments had antioxidant activities which may be used in functional food and cosmetics.
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PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 5
·1031
·
IF 5.2
Jing-Hong Liu,Yu-Yun Zheng,Jian-Ming Ouyang
The antioxidant activities of seven degraded products (GLPs) with different molecular weights (Mw) of polysaccharides fromGracilaria lemaneiformiswere compared. TheMwof GLP1–GLP7 were 106, 49.6, 10.5, 6.14, 5.06, 3.71 and 2.42 kDa, respectively. The results show that GLP2 withMw= 49.6 kDa had the strongest scavenging capacity for hydroxyl radical, DPPH radical, ABTS radical and reducing power. WhenMw< 49.6 kDa, the antioxidant activity of GLPs increased with the increase inMw, but whenMwincreased to 106 kDa, their antioxidant activity decreased. However, the ability of GLPs to chelate Fe2+ions increased with the decrease in polysaccharideMw, which was attributed to the fact that the polysaccharide active groups (–OSO3–and –COOH) were easier to expose, and the steric hindrance was smaller when GLPs chelated with Fe2+. The effects of GLP1, GLP3, GLP5 and GLP7 on the crystal growth of calcium oxalate (CaOx) were studied using XRD, FT-IR, Zeta potential and thermogravimetric analysis. Four kinds of GLPs could inhibit the growth of calcium oxalate monohydrate (COM) and induce the formation of calcium oxalate dihydrate (COD) in varying degrees. With the decrease inMwof GLPs, the percentage of COD increased. GLPs increased the absolute value of the Zeta potential on the crystal surface and reduced the aggregation between crystals. Cell experiments showed that the toxicity of CaOx crystal regulated by GLPs to HK-2 cells was reduced, and the cytotoxicity of CaOx crystal regulated by GLP7 with the smallestMwwas the smallest, which was consistent with the highest SOD activity, the lowest ROS and MDA levels, the lowest OPN expression level and the lowest cell necrosis rate. These results suggest that GLPs, especially GLP7, may be a potential drug for the prevention and treatment of kidney stones.Keywords:Gracilaria lemaneiformispolysaccharide;antioxidant activity;cytotoxicity;calcium oxalate;molecular weight
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PubMed
高影响因子
Food & Function
·
2021
·Vol. 12
·期 18
·8635-8646
·
IF 5.396
Xu-Ting Xie,Li-Xin Zheng,Hui-Min Duan,Yang Liu,Xian-Qiang Chen,Kit-Leong Cheong
Ulcerative colitis (UC) is a chronic lifetime disorder with a high incidence worldwide. A functional food-based method to prevent UC would be a good option for disease control. G. lemaneiformis oligosaccharides (GLOs) should have potent benefits for the gastrointestinal tract, based on in vitro fermentation assessed in our previous study. This study evaluated the therapeutic potential of GLOs in UC, as well as their possible mechanisms of action. The administration of GLOs was able to reduce the severity of dextran sulphate sodium-induced colitis by protecting mice from weight loss, reductions in colon length, inflammatory infiltration, and colon damage. Gut microbiota composition analysis showed that at the phylum level, GLOs could restore the composition of Bacteroidetes and decrease the level of Firmicutes. Consistently, it increased the contents of beneficial microbial metabolites and short-chain fatty acids in the mouse colitis model. In conclusion, GLOs could comprise a promising functional food strategy to alleviate UC symptoms.
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高影响因子
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