PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2015
·Vol. 32
·期 9
·886-892
·
IF 3.081
Junyu Lei,Xiaofeng Lu,Guangdi Nie,Ziqiao Jiang,Ce Wang
A facile one-pot synthetic route is reported to prepare algae-like molybdenum disulfide/polypyrrole (MoS 2 /PPy) nanocomposite through a redox reaction between ammonium tetrathiomolybdate and pyrrole monomer under a hydrothermal condition without any other templates. The as-prepared unique algae-like MoS 2 /PPy nanocomposites are composed of few layer MoS 2 nanosheets, which are covered with PPy. Structural and morphological characterizations of this unique nanocomposite are investigated by Fourier-transform infrared spectra, Raman spectra, X-ray diffraction pattern, X-ray photoelectron spectra, energy-dispersive X-ray spectroscopy, and transmission electron microscopy. The as-prepared MoS 2 /PPy nanocomposites exhibit an excellent peroxidase-like catalytic activity toward the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H 2 O 2 ) in acetate buffer solution (pH 4.0), which provides a facile strategy for the colorimetric detection of H 2 O 2 with a high sensitivity.
研究领域:
材料科学
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生物化学
·
分析化学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Maoqiang Chi,Guangdi Nie,Yanzhou Jiang,Zezhou Yang,Zhen Zhang,Ce Wang,Xiaofeng Lu
Here, we report a simple one-step procedure to fabricate coaxial Te@poly(3,4-ethylenedioxythiophene) (PEDOT) nanocables via a self-assembly redox polymerization between 3,4-ethylenedioxythiophene monomer and the oxidant of sodium tellurite without the assistance of any templates and surfactants. The as-synthesized Te@PEDOT coaxial nanocables have diameters of center cores in the range of 5–10 nm, and the size of the outer shell from several nanometers to 15 nm. More interestingly, the as-prepared Te@PEDOT nanocables can be converted to Pd@PEDOT nanocables via a galvanic replacement reaction. The center core of the Pd nanowire exhibits a high crystallinity. The application of the synthesized Pd@PEDOT nanocables as peroxidase-like catalysts for the colorimetric detection of H2O2 is reported. The synergistic effect between the Pd nanowire and electrically conducting PEDOT enhances the catalytic activity toward the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine in the presence of H2O2. A detection limit toward H2O2 is as low as 4.83 μM, and a linear range from 10 to 100 μM has been achieved. This work offers a potential versatile route for the fabrication of cable-like nanocomposites with conducting polymers and other active components, which display great promise in applications such as catalysis, nanoelectronic devices, and energy storage and conversion.
研究领域:
材料科学
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生物催化
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
ChemistrySelect
·
2025
·Vol. 10
·期 4
·e202405084
·
IF 2
Yaqi Kong,Xinjin Zhang,Chongchao Zhang,Ziyin Yang
Graphical In this work, nitrogen and ruthenium nanoparticles were anchored on the inner and outer surfaces of graphene hollow nanospheres (N/GHSs/Ru) using a template method to create nanomaterials with dual active sites. Because of the synergistic effect of the superfine Ru nanoparticles and N, N/GHSs/Ru nanomaterials showed excellent electrochemical performance for H 2 O 2 detection. The efficient construction of active sites is crucial for regulating the electrocatalytic activity of nanomaterials. In this work, nitrogen and ruthenium nanoparticles were anchored onto the inner and outer surfaces of graphene hollow nanospheres (N/GHSs/Ru) via the template method. Notably, the synergistic effect of the superfine Ru nanoparticles and N enhances the electrocatalytic activity of the N/GHSs/Ru nanomaterials, exhibiting excellent electrochemical performance for H 2 O 2 detection. N/GHSs/Ru nanomaterials were characterized by transmission electron microscopy (TEM), energy dispersion spectroscopy (EDS), field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and X-ray photoelectron spectrometry (XPS). The electrocatalytic reduction of H 2 O 2 showed a wide linear range from 0.02 to 5.6 mM, with a high sensitivity of 668.86 µA·mM −1 ·cm −2 and a limit of detection as low as 6.41 µM. This work offers a way to construct dual active sites, making N/GHSs/Ru as a promising material for nonenzymatic electrochemical H 2 O 2 sensors.
研究领域:
材料科学
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电化学传感器
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纳米技术
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产品关联
DOI
高影响因子
ANALYST
·
2012
·Vol. 137
·期 24
·5803-5808
·
IF 4.23
Chuantao Hou,Qin Xu,Lina Yin,Xiaoya Hu
Co3O4 nanoparticles (NPs) with an average diameter of about 20 nm were synthesized by using MOFs as a template. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed to characterize the as-prepared Co3O4 NPs. Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) were used to confirm the structure of the Co3O4 NPs. Then the Co3O4 NPs were modified on a glassy carbon electrode (GCE) to obtain a non-enzymatic glucose and H2O2 sensor. The NPs show electrocatalytic activity toward oxidation of glucose and H2O2 in alkaline medium. For glucose detection, the developed sensor shows a short response time (less than 6 s), a high sensitivity of 520.7 μA mM−1 cm−2, a detection limit of 0.13 μM (S/N = 3), and good selectivity. The high concentration of NaCl does not poison the electrode. Its application for the detection of glucose in a human blood serum sample shows good agreement with the results obtained from the hospital. Furthermore, the proposed sensor was used for the detection of H2O2. The results indicate that the detection limit and sensitivity for H2O2 are 0.81 μM and 107.4 μA mM−1 cm−2, respectively. Determination of H2O2 concentration in a disinfectant sample by the proposed biosensor also showed satisfactory result. The high sensitivity and low detection limit can be attributed to the excellent electrocatalytic performance of the as-prepared Co3O4 NPs. These results demonstrate that the as-prepared Co3O4 NPs have great potential applications in the development of sensors for enzyme-free detection of glucose and H2O2.
研究领域:
生物化学
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材料科学
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分析化学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Zeyu Wu,Linghao Zhao,Yangkun Sun,Yufeng Zhang,Yuerong Wang,Min Zhang,Hongyang Zhang,Fanghong Ning,Ping Hu
Hydrogen peroxide (H 2 O 2 ), a type of reactive oxygen species (ROS) produced by cells, plays a significant role in various physiological processes. Its abnormal concentrations in human body are closely associated with cellular damage as well as a variety of major diseases. Consequently, sensitive and accurate detection of cellular H 2 O 2 concentration is of great significance in evaluating the degree of intracellular oxidative stress in human cells. In this study, a novel heterogeneous nanomaterial, rGO@PDA@MXene-Au@COF, was designed for electrochemical-colorimetric dual-mode detection of intracellular H 2 O 2 , which enhances both peroxidase and electrochemical activities, achieving lower detection limits and broader linear ranges. ESR spectroscopy, EIS resistance analysis and computational simulation were employed to verify the mechanism of the activity enhancement, and the detection conditions were optimized. The methods were subsequently applied to the determination of H 2 O 2 in cells, yielding results of 1.69 × 10 −14 M H 2 O 2 /cell and 1.59 × 10 −14 M H 2 O 2 /cell for electrochemical and colorimetric detection of MCF-7 cells, and 1.67 × 10 −14 M H 2 O 2 /cell and 1.58 × 10 −14 M H 2 O 2 /cell of Hela cells, respectively. These results demonstrated high sensitivity and anti-interference capabilities, enabling quantitative detection of H 2 O 2 in complex cellular environments. Furthermore, the similarity in results between the two methods confirms their mutual accuracy and reliability. This work provided a novel approach for designing multifunctional nanomaterials and detecting H 2 O 2 in cells.
研究领域:
纳米技术
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电化学
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生物传感器
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分析化学
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细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
npj Parkinsons Disease
·
2025
·Vol. 11
·期 1
·1-10
·
IF 8.2
Lei Li,Chen Xiuli,Long Xincheng,Tu Qiuxia,Zhao Liping,Huang Dan,Song Lingli,Wang Jiaojiao,Zhai Suzhen,Chen Xiaozhong,Zhang Chunlin
Inspired by natural enzymes, artificial enzymes have garnered significant interest due to their simplicity of production, robustness under harsh conditions, and enhanced stability. This study introduces, for the first time, a novel Z/Ce@hemin composite enzyme, constructed by anchoring hemin onto zeolitic imidazolate framework-8 (ZIF-8)-encapsulated ceria (CeO 2 ) nanoparticles. This innovative design overcomes the challenges of hemin dimerization, enhances substrate affinity, and accelerates mass transport, leading to significantly improved peroxidase-like activity. The enzyme also demonstrates remarkable stability and resistance to environmental interference. Utilizing this Z/Ce@hemin composite, a sensitive and selective colorimetric detection system was developed for rapid and accurate quantification of hydrogen peroxide (H 2 O 2 ), a key oxidative stress marker. This approach was successfully applied in cellular models and Caenorhabditis elegans models of Parkinson’s disease, enabling precise monitoring of H 2 O 2 . The study provides a groundbreaking platform for oxidative stress modeling and expands possibilities in neurodegenerative disease research and therapeutic screening.
研究领域:
生物化学
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医学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 10
·2823-2830
·
IF 4.831
Zhong Yaping,Deng Chun,He Yu,Ge Yili,Song Gongwu
The authors describe a method for increasing the peroxidase-like catalytic properties of copper nanoclusters (Cu-NCs) that are used in non-enzymatic assays. The Cu-NCs were prepared by utilizing 6-thio-β-cyclodextrin as both the template and as effective modulators for increasing the peroxidase-like properties of the Cu-NCs. The β-CD-coated Cu-NCs have an average diameter of 2 nm, are stable in aqueous solutions, display strong fluorescence with excitation/emission peak wavelengths of 360/450 nm, and possess peroxidase-like catalytic activity which makes them a useful enzyme mimic. We have applied the findings to non-enzymatic photometric determination (at 650 nm) of (a) H 2 O 2 in the concentration range of 0.02 to 10 mM using the β-CD/Cu-NC assisted oxidation of tetramethylbenzidine by H 2 O 2 , and (b) glucose in the concentration range of 0.04 to 20 mM after addition of glucose oxidase and formation of H 2 O 2 . The detection limits (at an S/N ratio of 3) are 0.2 μM for H 2 O 2 and 0.4 μM for glucose. The β-CD coating is found to result in a strong increase in the reaction rate, probably because the cavity of β-CD acts as a pocket for the recognition and catalysis of substrate. Hence, the binding specificity becomes similar to that of natural enzymes.
The peroxidase-like catalytic activity of Cu-NCs in non-enzymatic sensors is strongly enhanced by utilizing mono-6-thio-β-cyclodextrin (mono-6-SH-β-CD) as both the template molecule and the effective rate modulator.
研究领域:
生物化学
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纳米医学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 33
·15721-15730
·
IF 3.3
Wanyu Song,Peng Sun,Jihong Zhou,Xu Han,Qijun Dai,Fang Chai
The Keggin phosphotungstic acid (PW12) embedded metal–organic framework copper benzene-1,3,5-tricarboxylate (CuBTC), CuBTC@PW12, was fabricated by a facile hydrothermal synthesis technique. With a unique tetrakaidecahedron structure and multiple active sites, the obtained CuBTC@PW12 exhibited peroxidase-mimicking activity, which promoted a novel strategy for qualitative and quantitative detection of hydrogen peroxide (H2O2) and sequential sensing of ascorbic acid (AA) via colorimetric evolution. Notably, with the advantages of strong redox performance of POMs and the unique porous structure of metal organic frameworks (MOFs), as peroxidase mimics, CuBTC@PW12 achieved highly selective and sensitive detection of H2O2via oxidizing TMB to a blue oxidation product (ox-TMB), with a limit of detection of 2.67 μM within the 0.01 to 1.0 mM scale. Concomitantly, the AA can be monitored consecutively by reducing the ox-TMB with fading blue color quantitatively with a limit of detection at 0.15 μM in the range of 0.01–0.10 μM. The recoveries for the determination of AA in human serum were obtained at 94.63–105%. These results manifested that CuBTC@PW12 could be used as a peroxide-mimetic enzyme for the continuous detection of H2O2 and AA with the advantages of simplicity, efficiency, sensitivity and accuracy.
研究领域:
材料科学
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分析化学
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生物医学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Wei Liu,Honghuang Li,Deming Li,Xiaoting Liu,Lei Liu,Zhiyong Zhu,Haoyu Hao,Qiongyao Zeng,Yanhua Sun
In this work, MWCNTs/Ti3C2 nanocomposites have been synthesized by a convenient method and characterized in terms of structure and properties by SEM, FT-IR, XPS and electrochemical methods. The electrochemical sensor developed by MWCNTs/Ti3C2 nanocomposites has good electrochemical properties. The sensor has good electrocatalytic performance for H2O2 and was used to detect the concentration of H2O2. The sublinear range of detection was 0.05-1080 μM and the detection limit was 0.017 μM (S/N=3). The sensor has strong anti-interference performance, stability and reproducibility, and has shown good performance in real sample detection.
研究领域:
材料科学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2024
·
IF 5.7
Shen Man,Dai Xianling,Ning Dongni,Xu Hanqing,Zhou Yang,Chen Gangan,Ren Zhangyin,Chen Ming,Gao Mingxuan,Bao Jing
The treatment of wound inflammation is intricately linked to the concentration of reactive oxygen species (ROS) in the wound microenvironment. Among these ROS, H2O2 serves as a critical signaling molecule and second messenger, necessitating the urgent need for its rapid real-time quantitative detection, as well as effective clearance, in the pursuit of effective wound inflammation treatment. Here, we exploited a sophisticated 3D Cu2-xSe/GO nanostructure-based nanonzymatic H2O2 electrochemical sensor, which is further decorated with evenly distributed Pt nanoparticles (Pt NPs) through electrodeposition. The obtained Cu2-xSe/GO@Pt/SPCE sensing electrode possesses a remarkable increase in specific surface derived from the three-dimensional surface constructed by GO nanosheets. Moreover, the localized surface plasma effect of the Cu2-xSe nanospheres enhances the separation of photogenerated electron-hole pairs between the interface of the Cu2-xSe NPs and the Pt NPs. This innovation enables near-infrared light-enhanced catalysis, significantly reducing the detection limit of the Cu2-xSe/GO@Pt/SPCE sensing electrode for H2O2 (from 1.45 μM to 0.53μM) under NIR light. Furthermore, this biosensor electrode enables in-situ real-time monitoring of H2O2 released by cells. The NIR-enhanced Cu2-xSe/GO@Pt/SPCE sensing electrode provide a simple-yet-effective method to achieve a detection of ROS (H2O2、-OH) with high sensitivity and efficiency. This innovation promises to revolutionize the field of wound inflammation treatment by providing clinicians with a powerful tool for accurate and rapid assessment of ROS levels, ultimately leading to improved patient outcomes.
研究领域:
生物医学工程
·
分析化学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2020
·
IF 7.632
Na Song,Ce Wang,Xiaofeng Lu
Recently, hybrid biocatalysts with a unique interfacial structure have attracted significant attention for enzyme mimicking due to their superior catalytic activity compared with the single component catalyst. In this work, novel sheet-on-tube heterostructured polypyrrole (PPy)@CoO/NiO nanotubes (NTs) are synthesized via an in situ growth strategy and are utilized for enzyme mimicking and sensing applications. The as-prepared PPy@CoO/NiO NTs can catalyze H2O2 into ·OH and realize the 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation, showing an excellent peroxidase-like property. Thanks to the synergistic effect between PPy NTs and CoO/NiO nanosheets, the catalytic performance of the resultant PPy@CoO/NiO NTs is superior to individual PPy NTs and bare CoO/NiO sheets as well as their physical mixture. In addition, the PPy@CoO/NiO NTs can also catalyze the H2O2 decomposing to generate O2, possessing a catalase-like property. Then, we perform a simple visual method for sensitively detecting H2O2 and ascorbic acid (AA). This work demonstrates a distinctive route to promote the catalytic performance of enzyme-like nanomaterials for huge potentials in biotechnology and environment fields.
研究领域:
材料科学
·
生物技术
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xin Xu,Peng Luo,Huan Yang,Shuang Pan,Hui Liu,Xiaoli Hu
This paper reports a facile approach for fabricating metal–adenosine triphosphate (ATP) nanoparticles (NPs) by the self-assembly of ATP and metal ions under moderate conditions. Here, Cu-ATP NPs only showed laccase-like activity, but the Fe 3+ -doped Cu-ATP NPs could exhibit peroxidase-like activity, resulting in a decrease in laccase-like activity. Mn 2+ ions functioned as a regulator to balance the enzyme-like activities of CuFe-ATP NPs, endowing the CuMnFe-ATP NPs with both strong laccase- and peroxidase-like activities. Surprisingly, the CuMnFe-ATP NPs also exhibited catalase-like activity under neutral conditions, improving the efficiency of phenol oxidation. X-ray photoelectron spectroscopy (XPS) analysis confirmed that the mixed valence states of the metals in the CuMnFe-ATP NPs accounted for the high catalytic efficiency; also, the large surface area and intrinsic porosity of the NPs were of great importance. The kinetic studies results indicated that the CuMnFe-ATP NPs exhibited rather strong affinities to 3,3,5′,5′-tetramethylbenzidine (TMB) and 2, 4-dichlorophenol (2,4-DP). On the basis of peroxidase-mimic activity, the CuMnFe-ATP NP system was employed to establish a facile colorimetric biosensing method for H 2 O 2 determination, and the detection limit of H 2 O 2 was 0.047 μmol⋅L −1 . These results prove that the enzyme-like activities of Cu-ATP NPs can be modulated via simple metal doping, which may extend to other nanozymes.
研究领域:
生物化学
·
纳米生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）