CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Yonglan Yang,Qianyu Cai,Liqiang Wang,Qiongmei Mai,Gang Ye,Jie Liu,Yanan Liu
Antibacterial Photocatalytic Therapy (APCT) is a promising therapeutic strategy for the treatment of bacterial infections, but it faces challenges such as low light utilization efficiency, insufficient reactive oxygen species (ROS) generation, and limited antibacterial efficacy. In this work, a novel Bi 2 MoO 6 @sRuO 2 @HA heterojunction (BMOsRH heterojunction) was constructed to address these limitations. The key innovation of this heterojunction lies in the introduction of sRuO 2 , which provides an effective charge carrier transfer interface for separated electrons and holes in Bi 2 MoO 6 , significantly delaying electron-hole recombination and promoting redox reactions that generate highly toxic ROS, thus enhancing antibacterial effects. Density functional theory (DFT) calculations indicated that BMOsRH possesses photocatalytic activity triply enhanced by near-infrared light absorption, defects, and the heterojunction. Consequently, under the combined action of near-infrared light and hydrogen peroxide, BMOsRH exhibited nearly 100 % antimicrobial activity against Escherichia coli (E. coli) and Methicillin-resistant Staphylococcus aureus ( MRSA ), and could also disrupt their biofilms. Furthermore, in mouse wound and abscess models of MRSA infection, BMOsRH effectively eliminated bacteria via mild photothermal therapy and significantly downregulated the inflammatory factors IL-1 β and TNF- α , promoting rapid wound healing. In summary, this light-controlled therapeutic strategy shows great potential for antibacterial applications.
研究领域:
微生物学
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材料科学
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医学
✓ 已验证
产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Lanlan Lin,Haiyang Li,Sisi Su,Xinyu Wen,Rui Yan,Caihong Tao
In this study, Fe 3 O 4 @HMPDA@HA core-shell submicron particles with a large specific surface area and total pore volumes were prepared. A large cavity structure was constructed between the core and the shell, so the core-shell particles as drug carriers have a large loading capacity. The adsorption properties and mechanism of organic dyes RhB and MB on particles were studied. The results showed that the adsorption capacities of the particles to RhB and MB were 830.65 mg/g and 380.66 mg/g respectively. And doxorubicin (DOX) was used as a targeted drug to research the delivery and sustained-release properties of the particles, the results showed that the loading capacity of DOX was up to 624.75 mg/g. In addition, Hyaluronic acid (HA) can specifically recognize the cancer cells, so the above prepared particles combined magnetic targeting and receptor-mediated targeting. In vitro experiments, the DOX loaded particles had a significant inhibitory effect on HeLa cells.
研究领域:
材料科学
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药物递送
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生物医学工程
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2025
·
IF 2.5
Zhaoxiang Zeng,Lihua Huang,Caiyan Xu,Siqi Rao,Yin Chen,Wenjin Xu,Hao Hong
Metal-organic frameworks (MOFs) are a unique type of coordination polymer characterized by their high porosity and enormous specific surface area, which enable their use as carriers. We have constructed a novel MOFs-based nanodelivery system designed for multimodal combination therapy to enhance the delivery efficiency and efficacy of chemotherapeutic drugs. The synthesis of MOF-199, which inherently possesses a drug-release response function, was used as a nanodrug carrier. Doxorubicin (DOX) was encapsulated through physical adsorption, and the carrier surface was modified with a biomimetic material, polydopamine (PDA). Both PDA and hyaluronic acid (HA) molecules contain carboxyl groups, which can be connected through Fe3+-mediated coordination interactions. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) were used to test the size, dispersity, and structural characteristics of the synthesized products, confirming their appropriate size and dispersity. BET(BET surface area) and TGA test results indicated that the nanocarrier exhibits good stability, a large specific surface area, and high porosity, making it suitable for use as a drug carrier. Ultraviolet spectrophotometry confirmed that the nanocarrier MOF-199-PDA-HA has the ability to consume GSH, with the drug loading rate and encapsulation efficiency of DOX in the system were measured as 39.58% and 73.26%, respectively. In cellular experiments, the DOX@MOF-199-PDA-HA group produced toxic hydroxyl radicals following H2O2 addition. After near-infrared light irradiation, both cellular toxicity and reactive oxygen species fluorescence intensity increased, indicating further enhanced anticancer efficacy. Using free HA as a control, it was confirmed that HA enhances tumor-specific accumulation of DOX@MOF-199-PDA-HA by binding to CD44 receptors on cancer cell surfaces.DOX@MOF-199-PDA-HA is a pH/GSH/NIR-responsive nanodelivery system with targeting functionality. This system ensures that drugs are released with higher efficiency in the tumor microenvironment through pH and glutathione (GSH) responsive drug-release mechanisms, thereby reducing toxicity to healthy tissues
研究领域:
药剂学
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纳米医学
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肿瘤学
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产品关联
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
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2022
·
IF 6.814
Guojuan Yan,Bo Kong,Jiaqiang Zhao,Huanhuan Ni,Lei Zhan,Chengzhi Huang,Hongyan Zou
The application of nanostructures to design fluorescence resonance energy transfer (FRET) based sensing platforms has been greatly concerned with the demand for sensitive and selective detection of biomolecules. Here, a novel sensitive turn-on fluorescence strategy based on the FRET mechanism has been designed for hyaluronidase (HAase) detection through the modulation of Cu 2-x Se@HA-Rh6G nanoprobe fabricated by self-assembly of rhodamine 6G (Rh6G) together with Cu 2-x Se@HA nanoparticles through electrostatic adsorption. The Cu 2-x Se@HA had extensive localized surface plasma resonance (LSPR) absorption in the wide range of ultraviolet (UV) to near-infrared (NIR) wavelengths and showed good light capture characteristics, which can be acted as good acceptors in the FRET interactions with Rh6G , inducing its efficient fluorescence quenching . In the presence of HAase, the FRET process was disrupted and the fluorescence signal was recovered. In the range of 0.1–10.0 U/mL, the fluorescence recovery of Rh6G showed a good linear relationship with the concentration of HAase, and the detection limit was 0.06 U/mL. The sensing platform has been used for HAase detection in real urine samples and cancer cells imaging.
研究领域:
纳米技术
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生物传感
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生物医学工程
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
DRUG DELIVERY
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2017
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IF 6.402
Chen-Lin Feng,Yan-Xing Han,Hui-Hui Guo,Xiao-Lei Ma,Zhi-Qiang Wang,Lu-Lu Wang,Wen-Sheng Zheng,Jian-Dong Jiang
Our previous work proved that sequence specific double strand RNA (dsRNA-p21) effectively activated p21 gene expression of colorectal cancer (CRC) cells and consequently suppressed CRC growth. However, efficient delivery system is a significant challenge to achieve sufficient therapy. In this study, a self-assembled HA/PEI/dsRNA-p21 ternary complex (TC-dsRNA-p21) was developed for the tumor-target delivery of dsRNA-p21 into CRC cells. Hyaluronic acid (HA) was introduced to shield the PEI/dsRNA-p21 binary complexes (BC-dsRNA-p21) for reducing the cytotoxicity of PEI and for increasing the tumor-targeted intracellular uptake by cancer cells through HA-CD44 mediated endocytosis. Comparing to the BC-dsRNA-p21, the TC-dsRNA-p21 showed increase in size, decrease in zeta potential, low cytotoxicity as well as high stability in physiological conditions due to the anionic shielding. Confocal microscopy analysis and flow cytometry confirmed that TC-dsRNA-p21 had high transfection efficiency in the CD44-abundant Lovo cells, as compared with binary complex. In vitro physiological experiment showed that, comparing to the control group, the TC-dsRNA-p21 effectively activated the expression of p21 mRNA and P21 protein, causing blockage of cell cycle at G0/G1 phase and suppression of cancer cell proliferation as well as colony formation. Furthermore, in vivo distribution experiment demonstrated that the TC-dsRNA-p21 could effectively accumulate at rectal wall for up to 10 h, following in situ application. These findings indicated that TC-dsRNA-p21 might hold great potential for delivering dsRNA-p21 to treat CRC.
研究领域:
生物医学工程
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肿瘤学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2021
·Vol. 13
·期 6
·934
·
IF 4.329
Huihui Yuan,Chenli Xue,Jiaqian Zhu,Zhaogang Yang,Minbo Lan
To reduce the possibility of bacterial infection and implant-related complications, surface modification on polyurethane (PU) film is an ideal solution to endow hydrophobic PU with antibacterial and antifouling properties. In this work, a variety of polyhexamethylene guanidine/ hyaluronic acid (PHMG/HA) multilayer films were self-assembled layer-by-layer on PU films using polyanions, carboxyl-activated HA, and polycations PHMG by controlling the concentration of these polyelectrolytes as well as the number of layers self-assembled. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) spectra, water contact angle (WCA), and A Atomic force microscope (AFM) of PU and modified PU films were studied. Protein adsorption and bacterial adhesion as well as the cytotoxicity against L929 of the film on selected PU-(PHMG/HA)5/5-5 were estimated. The results showed that PU-(PHMG/HA)5/5-5 had the best hydrophilicity among all the prepared films, possessing the lowest level of protein adsorption. Meanwhile, this film showed efficient broad-spectrum antibacterial performance as well as significant resistance of bacterial adhesion of more than a 99.9% drop for the selected bacteria. Moreover, almost no influence on cell viability of L929 enhanced the biocompatibility of film. Therefore, the modified PU films with admirable protein absorption resistance, antimicrobial performance, and biocompatibility would have promising applications in biomedical aspect.Keywords:modified PU film;protein adsorption;antibacterial;hyaluronic acid (HA);polyhexamethylene guanidine (PHMG)
研究领域:
材料科学
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生物医学工程
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表面化学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 8
·2042-2053
·
IF 6.331
Xiaolu Guo,Changchun Wen,Qianxin Xu,Changping Ruan,Xing-Can Shen,Hong Liang
The second near-infrared (NIR-II) window (1000–1350 nm) usually offers further improved light penetration, a higher maximum permissible exposure (MPE), and a lower background signal. Development of NIR-II optical diagnosis and phototherapy technologies is of great significance for precise, efficient tumor therapy. In this work, a new type of Ti-based targeting agent (B-TiO2@SiO2–HA) nanotheranostic system with strong NIR-II absorption was designed and fabricated for the first time. Oxygen vacancies were formed in B-TiO2 and its band gap was narrowed, resulting in nanotheranostic systems with full-spectrum responses to stimulation with light. The experimental results showed that B-TiO2@SiO2–HA not only can enable high NIR-II photothermal conversion and provide excellent reactive oxygen species (ROS) production capacity, but also can enable high-resolution photoacoustic imaging (PAI) under NIR-II laser irradiation. Moreover, HA modification gives the nanotheranostic systems the useful ability to target high-CD44-expression tumor cells and tissues. In vitro and in vivo experiments demonstrated that B-TiO2@SiO2–HA exhibited a targeted photothermal/photodynamic (PTT/PDT) effect that produced tumor-cell ablation and apoptosis under the guidance of real-time NIR-II PA imaging. B-TiO2@SiO2–HA exhibits precise nanotheranostic potential for PAI-guided tumor-targeting phototherapy.
研究领域:
生物医学工程
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材料科学
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癌症研究
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诊疗学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Xia Zhao,Hong Wang,Hong Peng,Linling Wang,Xiaohua Lu,Yanjun Huang,Jing Chen,Taikang Shao
Two buoyant ALG/HA/HGMs composite adsorbents, AHG(acid) and AHG(base), were prepared by encapsulating hollow glass microspheres and humate within alginate gel beads in this study. Both AHG(acid) and AHG(base) were characterized and their adsorption properties for removal of copper(II) ions from aqueous solution and kaolin soil were investigated. In aqueous solution, the maximum adsorption capacities of copper on AHG(acid) and AHG(base) were 34.8 and 40.4 mg g −1 , respectively, and the adsorption kinetics for AHG(acid) and AHG(base) followed the mechanism of the pseudo-second order kinetic model, and their equilibrium data were well fitted with the Langmuir isothermal model. The removal of copper from contaminated kaolin by AHG(acid) and AHG(base) was conducted by soil washing with deionized water containing buoyant adsorbents. As AHG(acid) could release hydrogen ions and reduce the pH value of the system consisting of adsorbent, water and kaolin, while AHG(base) could release hydroxide ions and increase the pH value of the system, the removal efficiency of copper from kaolin soil by AHG(acid) was much higher than that by AHG(base). Under soil washing conditions of the dosage of AHG(acid) of 3%, water/soil ratio of 3/1, and washing time of 48 h, the removal efficiency of copper from kaolin by AHG(acid) was 72.8%, and after 5 times reuse of AHG(acid) it was kept at 70%, while the pH value of kaolin soil was maintained in the pH range of 4.60–4.80. The results indicate that AHG(acid) has a potential application prospect in removing heavy metals from soil compared with AHG(base).
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
Practical Laboratory Medicine
·
2024
·
IF 1.7
Yuan Yu,Xiaoyun Zhang,Kai Lin
Background Most glycated hemoglobin A1c (HbA1c) analytical reagents used were obtained from the analyzer's manufacturer. However, clinical laboratories need more choices for HbA1c analytical reagents to overcome the limitations of dedicated reagents for special analyzers. We developed new mobile phase buffers as HbA1c diagnostic reagents and evaluated their analytical performance for the HbA1c assay. Methods Different mobile phase buffers used as HbA1c diagnostic reagents were prepared using different concentrations of sodium salts. According to the Clinical and Laboratory Standards Institute (CLSI) recommendation guidelines, the analytical performances of the newly developed mobile phase buffers were evaluated on an ARKRAY HA-8160 Analyzer. Both quality controls and clinical blood samples were used in these experiments. To assess the quality of the newly developed mobile phase buffers, precision, accuracy, linearity, carryover, interference, bias, correlation with commercial reagents, and stability were analyzed. Results The CV s of intra-assay precision and interassay precision of quality control and clinical. There were fewer than 1.00 % blood sample assays using the newly developed mobile phase buffer. The RDs of accuracy were less than 1.00 %. Linearity: R 2 = 0.9998 in the concentration range of 4.40%–17.30 %. Carryover: 0.00 %. Reagent comparison revealed that the Pearson regression equation was Y = 0.9884x+0.05692 (R 2 = 0.9977), and the Bland-Altman mean difference was −0.02650 % (CI: −0.2121 %–0.1591 %) between the two analytical reagents. Stability was also acceptable within 12 months. This mobile phase buffer showed good anti-interference ability. Conclusion The newly developed mobile phase buffers demonstrated good analytical performance and were suitable for clinical HbA1c assays on an ARKRAY HA-8160 Analyzer.
研究领域:
临床检验学
·
医学实验室科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Drug Design Development and Therapy
·
2024
·
IF 4.7
Chaoying Jin,Ruolin Zhao,Weihang Hu,Xiaolong Wu,Li Zhou,Letian Shan,Huiling Wu
Background Pressure ulcer is common in the bedridden elderly with high mortality and lack of effective treatment. In this study, human-adipose-derived-stem-cells-hyaluronic acid gel (hADSCs-HA gel) was developed and applied topically to treat pressure ulcers, of which efficacy and paracrine mechanisms were investigated through in vivo and in vitro experiments.Methods Pressure ulcers were established on the backs of C57BL/6 mice and treated topically with hADSCs-HA gel, hADSCs, hyaluronic acid, and normal saline respectively. The rate of wound closure was observed continuously during the following 14 days and the wound samples were obtained for Western blot, histopathology, immunohistochemistry, and proteomic analysis. Human dermal fibroblasts (HDFs) and human venous endothelial cells (HUVECs) under normal or hypoxic conditions were treated with conditioned medium of human ADSCs (ADSC-CM), then CCK-8, scratch test, tube formation, and Western blot were conducted to evaluate the paracrine effects of hADSCs and to explore the underlying mechanism.Results The in vivo data demonstrated that hADSCs-HA gel significantly accelerated the healing of pressure ulcers by enhancing collagen expression, angiogenesis, and skin proliferation. The in vitro data revealed that hADSCs strengthened the proliferation and wound healing capabilities of HDFs and HUVECs, meanwhile promoted collagen secretion and tube formation through paracrine mode. ADSC-CM was also proved to exert protective effects on hypoxic HDFs and HUVECs. Besides, the results of proteomic analysis and Western blot elucidated that lipid metabolism and PPARβ/δ pathway mediated the healing effect of hADSCs-HA gel on pressure ulcers.Conclusion Our research showed that topical application of hADSCs-HA gel played an important role in dermal regeneration and angiogenesis. Therefore, hADSCs-HA gel exhibited the potential as a novel stem-cell-based therapeutic strategy of treating pressure ulcers in clinical practices.
研究领域:
生物医学工程
·
干细胞生物学
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组织工程
·
伤口愈合
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Yafang Chen,Junhui Sui,Qing Wang,Yijia Yin,Jun Liu,Qiguang Wang,Xianglong Han,Yong Sun,Yujiang Fan,Xingdong Zhang
The injectable self-crosslinking blend hydrogel by combination of collagen I and thiolated hyaluronic acid could alleviate collagen I contraction in vitro and overcome weak cell adhesive sites of hyaluronic acid. Five groups of injectable hydrogels with different ratios were prepared to investigate their gelation time, injection force, mechanical properties, swelling capacity and disintegration performance. These results indicated that Col 7 HA-SH 3 hydrogel achieved the optimal controlled and injectable effect, the gelation time was just ten seconds with injection force at 3.5 N, and the storage modulus of hydrogel could reach 11 kPa with frequency at 10 Hz. Furthermore, the phenotype maintaining, biocompatibility and chondrocytes proliferation were administrated by CLSM, SEM, histological staining, immuohistochemical staining, MTT test and glycoaminoglycans quantification. Similarly, the Col 7 HA-SH 3 blend hydrogel encapsulated chondrocytes presented most excellent proliferation potential, phenotype maintaining, biocompatibility and convenient operational characteristics. These findings might approach the underlying clinical application of blend hydrogel in cartilage repair.
研究领域:
组织工程
·
生物材料科学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Huachu Wu,Liang Shen,Zhu Zhu,Xusong Luo,Yishu Zhai,Xiaobin Hua,Shicheng Zhao,Lian Cen,Zhibing Zhang
This study was to co-encapsulate a chemokine (stromal cell-derived factor-1, SDF-1) and a chondroinductive molecule (kartogenin, KGN) within microspheres via microfluidics, and to incorporate them into a hyaluronic acid (HA) injectable scaffold for articular cartilage defect repair. HA injectable scaffold, as a cartilage-friendly microenvironment, was prepared by crosslinking HA with 1,4-butanediol diglycidyl ether. A microfluidic device was set up to prepare monodisperse PLGA microspheres (49 μm) to load SDF-1 and KGN. An in vivo model of full-thickness articular cartilage defects in rabbits was applied to evaluate the reparative capacity of the current package. The SDF-1 and KGN were co-encapsulated simultaneously within the core and shell area of the microsphere with high loading efficiency and sustained release profiles of more than 2 months. The release profiles of them were highly matched and well fitted to a first-order mathematical model. These microspheres when incorporated into HA injectable scaffold were demonstrated to heal the full-thickness articular cartilage defects in rabbits. The regenerated tissue had the typical cartilage histological characters and integrated well with the surrounding tissue at 12w. This developed cell-free system could serve as an efficient therapy for articular cartilage defects treatment, serving as a supplementary way to cell based therapies.
研究领域:
生物医学工程
·
组织工程
·
骨科
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）