Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Lingjie Liu,Shaoqing Mo,Shaopo Wang,Jing Chang,Jianhui Zhao,Jingjie Yu
Low temperatures were a critical factor that inhibited anaerobic ammonium oxidizing bacteria (AnAOB) activity. This study demonstrated the effect of exogenous zero-valent iron (ZVI) on Anammox-hydroxyapatite (HAP) system at 17°C. The results showed that long-term addition of ZVI increased the system nitrogen removal efficiency by 8.59% and alleviated the inhibition of AnAOB by low temperature. After addition of ZVI, the activity of AnAOB and the heme c content increased by 58.20% and 4.83%, respectively. Moreover, the functional enzyme activities of NIR, HZS, and HZO increased by 94.12%, 36.36%, and 16.67%, respectively. The concentrations of quorum sensing molecules, i.e., C 6 -HSL and C 8 -HSL, increased by 191.17% and 41.54%, respectively, after ZVI addition. Metagenomic analysis revealed that the addition of 5 g/L ZVI stimulated the relative abundance relative abundance of Fe(II) metabolism-related genes in AnAOB at 17 °C, thereby contributing to the uptake, transport, utilization, and storage of Fe(II). The addition of ZVI could improve the nitrogen removal performance of the anammox-HAP system at the low temperature.
研究领域:
环境工程
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Advances
·
2024
·
IF 5.5
Xiaomei Dai,Meijuan Yuan,Yuxia Yang,Mengbo Dang,Jian Yang,Junli Shi,Dianwei Liu,Mingjun Li,Hang Yao,Wenyong Fei
The tendon-bone interface heals through scar tissue, while the lack of a natural interface gradient structure and collagen fibre alignment leads to the occurrence of retearing. Therefore, the promotion of tendon healing has become the focus of regenerative medicine. The purpose of this study was to develop a gradient COL1/ hydroxyapatite (HAp) biomaterial loaded with human amniotic mesenchymal stem cells (hAMSCs). The performance of common cross-linking agents, Genipin, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS), and dual cross-linked materials were compared to select the best cross-linking mechanism to optimize the biological and mechanical properties of the scaffold. The optimal COL1/HAp-loaded with hAMSCs were implanted into the tendon-bone rotator cuff interfaces in rats and the effect on the tendon-bone healing was assessed by micro-CT, histological analysis, and biomechanical properties. The results showed that Genipin and EDC/NHS dual cross-linked COL1/HAp had good biological activity and mechanical properties and promoted the proliferation and differentiation of hAMSCs. Animal experiments showed that the group using a scaffold loaded with hAMSCs had excellent continuity and orientation of collagen fibers, increased fibrocartilage and bone formation, and significantly higher biomechanical functions than the control group at the interface at 12 weeks post operation. This study demonstrated that dual cross-linked gradient COL1/HAp-loaded hAMSCs could promote interface healing, thereby providing a feasible strategy for tendon-bone interface regeneration.
研究领域:
再生医学
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组织工程
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生物材料
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干细胞生物学
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Liangting Shao,Jingwen Wang,Haijing Hu,Xinglian Xu,Huhu Wang
Microbial spoilage of meat products is a significant problem in the food industry. Aeromonas salmonicida is a significant microorganism responsible for spoilage in chilled meat. Its effector protein, hemagglutinin protease (Hap), has been identified as an effective substance for degrading meat proteins. The ability of Hap to hydrolyze myofibrillar proteins (MPs) in vitro demonstrated that Hap has obvious proteolytic activity, which could alter MPs’ tertiary structure, secondary structure, and sulfhydryl groups. Moreover, Hap could significantly degrade MPs, focusing primarily on myosin heavy chain (MHC) and actin. Active site analysis and molecular docking revealed that the active center of Hap was bound to MPs via hydrophobic interaction and hydrogen bonding. It may preferentially cleave peptide bonds between Gly44-Val45 in actin, and Ala825-Phe826 in MHC. These findings suggest that Hap may be involved in the spoilage mechanism of microorganisms and provide crucial insights into the mechanisms of meat spoilage induced by bacteria.
研究领域:
食品科学
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微生物学
·
蛋白质化学
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结构生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
·
2018
·
IF 1.2
Feigao Xu,Changyi Jiang,Dan Li
Hydroxyapatite (HAP)-coated-limestone was applied to remove fluoride from aqueous solution. The adsorbent was prepared by reaction of limestone and diammonium hydrogen phosphate with or without ethanol. The results of XRD and BET analyses both showed that with the addition of ethanol, the structure of HAP-coated-limestone has been changed, resulting in changes of its crystallinity and specific surface area. Results of removing fluoride indicate that adding small amounts of ethanol (3 vol%) to the formulation remarkably increases fluoride removal. The possible mechanisms for F− adsorption onto HAP-coated-limestone surface have been proposed in this paper.
研究领域:
环境科学
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水处理
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吸附技术
·
材料科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Guozhen Wang,Tong Lu,Xinjiang Zhang,Mengna Feng,Chengshuang Wang,Wei Yao,Shunchao Zhou,Zhenzhou Zhu,Wenping Ding,Meng He
The exploiting of abundant natural polymers as potential absorbents for heavy metal ions is attracting. Cellulose is the most abundant natural polymer and exhibits amazing properties such as high chemical stability, hydrophilicity and biodegradability . However, some properties of pure cellulose-based materials including adsorbability are usually not enough, so it is important to improve their properties to broaden their applications. In the present work, hydroxyapitite (HAP) nanoparticles were prepared and introduced to improve the cellulose hydrogel (CG) properties. The structure and properties of the resultant cellulose/HAP nanocomposite hydrogels (CHG) were characterized and studied systematically. The results indicated that HAP nanoparticles was fixed and distributed evenly in CG. The maximum decomposition temperature increased gradually from 334.6 °C for CG to 346.7 °C for CHG, and the compressive strength increased gradually from 100 kPa for CG to 570 kPa for CHG with the increase of HAP content, respectively. Moreover, the adsorption capacity (q e ) value of CHG towards Cu 2+ could reach more than 300% higher than that of CG. As a potential absorbent, CHG exhibited relatively good recyclability of more than 78% after 10 cycles. Therefore, the introduction of HAP improved the properties of CG greatly, showing wide potential applications.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2020
·Vol. 324
·期 3
·1329-1337
·
IF 1.137
Zeng Dejun,Dai Ying,Zhang Zhibing,Wang Youqun,Cao Xiaohong,Liu Yunhai
In this work, the magnetic hydroxyapatite composite (Fe 3 O 4 @HAP) was prepared and used to remove of U(VI). The characterizations of transmission electron microscope and vibrating sample magnetometer indicated that the average size and saturation magnetizations ( M s ) of Fe 3 O 4 @HAP was about 360 nm and 20.89 emu g −1 , respectively. The results of solid-phase extraction indicated that at pH 5.0, the removal efficiency of Fe 3 O 4 @HAP was more than 95% within 20 min. The maximum adsorption capacity ( q m ) of Fe 3 O 4 @HAP was 789.58 mg g −1 . Therefore, Fe 3 O 4 @HAP may be a rapid and highly efficient magnetic adsorbent to remove of U(VI) from water.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
WATER SCIENCE AND TECHNOLOGY
·
2016
·Vol. 74
·期 1
·276-286
·
IF 1.064
Huang Bin,Xiong Dan,Zhao Tingting,He Huan,Pan Xuejun
Biomorphic nano-hydroxyapatite (HAP) was fabricated by a co-precipitation method using cotton as bio-templates and employed in adsorptive removal of ofloxacin (OFL) and triclosan (TCS) that are two representative pharmaceuticals and personal care products (PPCPs). The surface area and porosity, crystal phase, functional group, morphology and micro-structure of the synthesized HAP were characterized by Brunauer–Emmett–Teller isotherm, X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning electron macroscopic and transmission electron microscopy. The effects of initial pH, ionic strength, initial concentration, contact time and temperature on the removal of PPCPs were studied in a batch experiment. The adsorption of OFL and TCS was rapid and almost accomplished within 50 min. Kinetic studies indicated that the adsorption process of OFL and TCS followed the pseudo-first-order and pseudo-second-order models, respectively. The Freundlich isotherm described the OFL adsorption process well but the adsorption of TCS fitted the Langmuir isotherm better. Thermodynamics and isotherm parameters suggested that both OFL and TCS adsorption were feasible and spontaneous. Hydrogen bond and Lewis acid–base reaction may be the dominating adsorption mechanism of OFL and TCS, respectively. Compared to other adsorbents, biomorphic HAP is environmentally friendly and has the advantages of high adsorption capacity, exhibiting potential application for PPCPs removal.
研究领域:
环境科学
·
材料科学
·
纳米技术
·
环境污染与控制
·
吸附材料
✓ 已验证
DOI
高影响因子
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2020
·Vol. 97
·期 1
·138-145
·
IF 2.008
Ge Zhenying,Li Ping,Li Binjie,Zhao Yanbao
Hydroxyapatite/silver/silver sulfadiazine ternary nanocomposite (HAP/Ag/SSD TNC) with a novel hierarchical structure was successfully fabricated as following steps. First, mesoporous hydroxyapatite (HAP) nanorods as carrier were synthesized by a facile hydrothermal method. Second, as one antibacterial agent, silver nanoparticles were designed to be loaded on the mesoporous HAP nanorods to produce HAP/silver nanorods. Third, silver sulfadiazine as the other antibacterial agent was in situ produced on the surface of HAP/silver nanorods to form HAP/Ag/SSD TNC. To estimate its antibacterial properties, minimum inhibitory concentration, minimal bactericidal concentration, and a filter paper inhibition zone experiments were conducted with three bacteria strains of gram-negative Escherichia coli ( E. coli ), Pseudomonas aeruginosa ( P. aeruginosa ), and gram-positive Staphylococcus aureus ( S. aureus ). The results demonstrated that HAP/Ag/SSD TNC exhibited outstanding and sustained antibacterial activities against P. aeruginosa in 48 h. Toward E. coli and S. aureus , HAP/Ag/SSD TNC displayed similar antibacterial abilities with that of HAP/silver nanorods. Therefore, HAP/Ag/SSD TNC has the potential to be a new excellent antibacterial agent and extensively applied in the antibacterial field.
Illustration of the general formation procedure of HAP/Ag/SSD TNC.
研究领域:
材料科学
·
生物学
·
医学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2019
·
IF 4.561
Xilong Peng,Wenjin Chen,Zongjian He,Dan Li,Heng Liu,Hong Jin,Guigen Zhou,Feigao Xu
Reducing copper content in copper-containing water is a worldwide environmental problem. It has been shown that hydroxyapatite (HAP) has a significant adsorption effect on heavy metals (including Cu 2+ ). In this study, doped HAP-coated-limestone was synthesized using waste limestone, diammonium hydrogen phosphate and two kinds of chloride (Mg 2+ , Sr 2+ ). The materials were characterized by X-ray diffraction (XRD), Fourier transform infrared spectrophotometry (FTIR), thermogravimetric analysis (TGA), Brunner-Emmet-Teller (BET) measurements and scanning electron microscope (SEM). It was found that the structure of Mg 2+ and Sr 2+ doped HAP-coated-limestone was similar to that of HAP-coated-limestone. The surface of doped HAP-coated-limestone is rougher, and the specific surface area is larger than that of HAP-coated-limestone. Some factors have been investigated affecting the uptake behavior, such as doped element, contact time, temperature and Cu 2+ concentration to optimize the conditions. The adsorption isotherms of Langmuir and Freundlich were studied, and it was proved that the adsorption isotherm of Langmuir was more consistent with the adsorption process of doped HAP-coated-limestone. The quasi-second-order dynamics can be better used to describe the experimental results compared with quasi-first-order dynamics. At the temperature of 30 °C, the adsorption capacity of HAP-coated-limestone for Cu (II) is 90.90 mg·g −1 , while the adsorption capacity is 214.20 and 121.61 mg·g −1 for Mg 2+ and Sr 2+ doped HAP-coated-limestone, respectively. Through the calculation of thermodynamic parameters, the results show that the adsorption process of Cu (II) on HAP-coated-limestone and doped HAP-coated-limestone becomes easier with the increase of temperature in a certain temperature range. The adsorption process on HAP-coated-limestone and doped HAP-coated-limestone is spontaneous and endothermic.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Catalysis
·
2017
·
IF 10.614
Guangyue Xu,Ying Zhang,Yao Fu,Qingxiang Guo
A catalytic system over Ru-HAP catalyst is established to hydrodeoxygenate various oils to long-chain alkanes in water, for potential large-scale renewable diesel production, which has the following advantages. (i) This system is versatile to different oil sources, including Jatropha oil, palm oil, waste cooking oil, and cooking waste. (ii) Ru-HAP is highly efficient at achieving full conversion from stearic acid to alkanes at as low as 100 °C, and the isolated yield from Jatropha oil, palm oil, and waste cooking oil to long-chain alkanes reached up to 95, 96, and 87 mol % at 180 °C and 2 MPa H2 within 4–4.5 h, respectively. (iii) The catalyst showed high stability during five runs of recycling, ICP-OES analysis, and a hydrothermal treatment. The activity decreased less than 5% after the catalyst was treated in water at 200 °C for 24 h with a stirring speed of 1000 rpm due to the strong metal and hydrothermally stable support interaction. (iv) Ru-HAP is compatible with most impurities such as various salts, sugars, and macromolecules. (v) The system required low cost for operation since no dehydration before the reaction was necessary and the alkane product can be easily separated from water. The reaction route was investigated and indicated that the coexisting hydrodehydration and hydrodecarbonylation are affected by water, temperature, and H2 pressure. The catalyst was also characterized in detail, and its high reactivity and stability may result from the fact that highly distributed Ru nanoclusters anchored on the HAP support absorbed fatty acids by forming a metastable calcium carboxyl phosphate.
研究领域:
催化化学
·
可再生能源
✓ 已验证
DOI
高影响因子
COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
·
2024
·
IF 8.1
Xingyu Gui,Boqing Zhang,Yuxiang Qin,Haoyuan Lei,Xiuwen Xia,Yiyang Li,Haoxiang Lei,Xuerui Zhou,Yanfei Tan,Zhihong Dong,Qi You,Changchun Zhou,Yujiang Fan
Critical bone defects caused by trauma, bone tumors, and infections are still challenges in clinical surgery. Digital light processing printing of hydroxyapatite (HAp) scaffolds can fabricate high-resolution personalized bone repair scaffolds. However, creating load-bearing bone regeneration HAp scaffolds with satisfactory mechanical property remain challenging. This study investigated the enhancement of the HAp scaffold mechanical properties from both structural and material perspectives. Structurally, the diamond structure model was optimized to Triply Periodic Minimal Surface structures, increasing the compressive strength of scaffolds from 2.2 to 7.2 MPa. Material-wise, ZnO was incorporated as second-phase particle, improved the compressive strength of HAp scaffolds from 7.2 to 13.7 MPa. Additionally, the incorporation of zinc ions enhanced the bioactivity of the HAp scaffolds. Animal mechanical testing revealed that the enhanced scaffolds effectively supported load-bearing. The HAp/ZnO-TPMS scaffolds exhibited excellent mechanical properties and promoted cellular osteogenic differentiation, showing potential for clinical development and application.
研究领域:
生物材料学
·
组织工程
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2026
·
IF 6.2
Zhenbiao Wangzi,Chengcheng Yang,Peng Yu,Xiubiao Huang,Nan Hu,Tuo Jiao,Hao Qi
Osteochondral defects require the simultaneous regeneration of cartilage and subchondral bone, posing a major challenge for current biomaterial strategies. Here, we present a radially oriented 3D-printed bilayer hydrogel scaffold composed of a mechanically reinforced GelMA/HAP osteogenic layer and a compliant, KGN@Lip-loaded GelMA chondrogenic layer, to achieve coordinated osteochondral repair. Structural and physicochemical characterization confirmed the formation of a functionally graded architecture with uniform HAP and KGN@Lip distribution, while mechanical, rheological, swelling, and degradation analyses revealed a layer-dependent gradient that resembles certain structural features of the native osteochondral interface. In vitro, the GelMA/HAP layer promoted robust osteogenic differentiation through ion-mediated activation of osteogenic pathways, whereas the GelMA/KGN@Lip layer sustained chondrogenic stimulation and markedly enhanced SOX9, Col2a1, and Acan expression. The radially oriented microchannels provided an interconnected structure that may support nutrient transport and cell infiltration. In vivo implantation demonstrated substantial subchondral bone regeneration and the formation of cartilage-like tissue with improved matrix organization, along with enhanced integration at the osteochondral interface, confirming the scaffold's ability to provide spatially coordinated biochemical and structural cues. Overall, this anisotropic bilayer hydrogel scaffold offers a promising strategy for integrated and functional osteochondral regeneration.
✓ 已验证
DOI
PubMed
高影响因子
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