JOURNAL OF CHROMATOGRAPHY A
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2022
·
IF 4.601
Yuting Li,Luhan Wang,Minhua Qian,Shengda Qi,Lei Zhou,Qiaosheng Pu
ABSTRACT γ-Hydroxybutyric acid (GHB), a neurotransmitter or neuromodulator in the human central nervous system, is often abused in drug-facilitated sexual assaults due to its euphoric and sedative effects. While the analysis of GHB has received continuous attention, its inherent characteristics pose challenges. In the current study, capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C 4 D) was built, and Good's buffers were evaluated as the background electrolytes for CE separation and C 4 D detection. On this basis, a simple and efficient CE-C 4 D method was developed for GHB analysis. Through theoretical discussion and experimental optimization, the separation of GHB and related positional isomers α-hydroxybutyric acid (AHB) and β-hydroxybutyric acid (BHB) was achieved within 4 min using 150 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) as the running buffer. Under the optimized condition, the relative standard deviations of migration time and peak area were less than 1.1% and 4.5%, indicating good precision. The C 4 D signal of GHB showed a good linear relationship with GHB concentration in the range of 3-300 µM with a determination coefficient of 0.9997, and the detection limit was calculated to be 0.37 µM based on the signal-to-noise ratio of three. Furthermore, liquid-liquid extraction (LLE) and solid-phase extraction (SPE) were comparatively studied for sample matrix purification. Combined with the optimized SPE procedure, the developed CE-C 4 D method has been successfully applied for the determination of exogenous GHB in spiked beverages and endogenous GHB in human urine.
研究领域:
分析化学
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法医学
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神经科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
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IF 4.244
Li Yang,Yonghai Song,Mulan Zeng,Yue Du,Bingxian Peng,Zhenzhong Huang,Li Wang
It is of great significance for superoxide anion (O 2 •− ) detection due to its important role in regulation of biological function. The nanoscale coordination polymers (NCPs) core-shell nanostructures have attracted increasing interests for their nanosized-dependent properties. In this study, SiO 2 @Tb/guanosine 5′-monophosphate (GMP) NCPs with core-shell nanostructures was designed for O 2 •− detection. The SiO 2 @Tb/GMP NCPs core-shell nanostructures were produced by using SiO 2 nanoparticles (NPs) as template to control their nano-size. First, Tb 3+ was adsorbed on the surface of SiO 2 NPs by coordination with -COOH of SiO 2 NPs. Then the Tb/GMP NCPs shells were grown along the surface of SiO 2 NPs. The SiO 2 @Tb/GMP NCPs core-shell nanostructures were characterized with scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and Zeta potential. The resulted SiO 2 @Tb/GMP NCPs core-shell nanostructures displayed strong Tb/GMP emission which decreased linearly with the increased O 2 •− concentration in the range from 5.3 nM to 6.0 μM with a limit detection of 2.18 nM. And the SiO 2 @Tb/GMP NCPs core-shell nanostructures also exhibited good selectivity and high sensitivity in HEPES buffer at pH 7.4.
研究领域:
材料科学
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分析化学
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生物化学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2021
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IF 10.618
Zipin Zhang,Rui Zhou,Yongliang Dong,Mengmeng Song,Lei Tian,Zuzhi Zhao
We have reported an optical indicator displacement assay (IDA) for heparin with a UV–vis absorbance and fluorescence dual-readout based on pyranine/methyl viologen (MV 2+ ). Upon introducing heparin, pyranine/MV 2+ shows a clearly observable increase in UV–vis absorbance and a turn-on of the fluorescence signal. We have demonstrated that the ionic nature of buffers significantly affects the pyranine displacement and the zwitterionic HEPES was most suitable for heparin sensing. After careful screening of experimental conditions, the pyranine/MV 2+ -based optical chemosensor exhibits a fast, sensitive, and selective response toward heparin. It shows dynamic linear concentration of heparin in the ranges of 0.1–40 U·mL −1 and 0.01–20 U·mL −1 for the absorptive and fluorescent measurements, respectively, which both cover the clinically relevant levels of heparin. As with the animal experiments, the optical chemosensor has been demonstrated to be selective and effective for heparin level qualification in rat plasma. The chemosensor is readily accessible, cost-effective, and reliable, which holds a great promise for potential application on clinical and biological studies. Furthermore, this IDA system can serve as an IMPLICATION logic gate with a reversible and switchable logical manner.
研究领域:
分析化学
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生物化学
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分子生物学
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临床化学
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产品关联
DOI
PubMed
高影响因子
Biofabrication
·
2021
·Vol. 13
·期 2
·025002
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IF 10.02
Zeyang Liu,Hongyong Zhang,Zhen Zhan,Haochen Nan,Nan Huang,Tao Xu,Xiaohua Gong,Chengzhi Hu
Internal gelation has been an important sol-gel route for the preparation of spherical microgel for drug delivery, cell therapy, or tissue regeneration. Despite high homogeneity and permeability, the internal gelated microgels often result in weak mechanical stability, unregular interface morphology and low cell survival rate. In this work, we have extensively improved the existing internal gelation approach and core–shell hydrogel microcapsules (200–600 μm) with a smooth surface, high mechanical stability and cell survival rate, are successfully prepared by using internal gelation. A coaxial flow-focusing capillary-assembled microfluidic device was developed for the gelation. Rapid gelling behavior of alginate in the internal gelation makes it suitable for producing well-defined and homogenous alginate hydrogel microstructures that serve as the shell of the microcapsules. 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES) was used in the shell stream during the internal gelation. Thus, a high concentration of acid in the oil solution can be used for better crosslinking the alginate while maintaining high cell viability. We further demonstrated that the gelation conditions in our approach were mild enough for encapsulating HepG2 cells and 3T3 fibroblasts without losing their viability and functionality in a co-culture environment.
研究领域:
生物医学工程
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组织工程
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细胞治疗
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药物递送
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2019
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IF 3.206
Fuchu Gong,Wu Zou,Qinge Wang,Renxing Deng,Zhong Cao,Tingting Gu
Excited-state intramolecular proton transfer (ESIPT)-exhibiting polymer nanoparticles (e-PNPs) were prepared using 2-hydroxy-5-methylisophthalaldehyde and melamine as reactants. These e-PNPs possess abundant aromatic o -OH, C N and NH 2 groups, which are responsible for ESIPT modules and molecular recognition . Compared with small ESIPT compounds, e-PNPs displayed ultrabright luminescence and high fluorescence quantum yield owing to their rich ESIPT-chromophores and appropriate hydrophobic environment for ESIPT reaction. The e-PNPs can emit strong green fluorescence and exhibit typical dual emission in a HEPES buffer (20 mM, pH 7.5). Upon the addition of VB 1, the ESIPT reaction of e-PNPs was inhibited, eliciting obvious decrease in ESIPT fluorescence. By making use of this signaling mechanism, a tautomeric fluorescence quenching-based method for detecting VB 1 was established. A good linear range of 0.1–25 μM.L −1 was observed, bringing about an excellent detection limit of 2.6 nM.L −1 . Moreover, this assay shows good selectivity over other water-soluble vitamins and some biologically related species. The employment of e-PNPs for the determination of VB 1 in kiwi and grape juice samples showed remarkable abilities in terms of the simplicity, stability, reproducibility and sensitivity, which conforms its great potential for real sample analysis. Therefore, e-PNPs can offer a simple, highly sensitive and selective sensing model for fluorescence detection of VB 1 .
研究领域:
材料科学
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分析化学
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纳米技术
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生物技术
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Lianshun Zhang,Mengqing Jia,Xi Wang,Lei Gao,Bo Zhang,Lei Wang,Jinming Kong,Lianzhi Li
It is of great significance to sensitively and selectively detect uranyl ion (UO 2 2+ ) in environmental and biological samples due to the high risks of UO 2 2+ to human health. However, such suitable sensors are still scarce. A novel fluorescence sensor based on a dansyl-modified peptide, Dansyl-Glu-Glu-Pro-Glu-Trp-COOH (D-P5), was efficiently synthesized by Fmoc solid phase peptide synthesis. As the first linear peptide-based fluorescence sensor for UO 2 2+ , D-P5 exhibited high selectivity and sensitivity to UO 2 2+ over 27 metal ions (UO 2 2+ , Cr 3+ , Cu 2+ , Ba 2+ , Hg 2+ , Pb 2+ , Co 2+ , Ag + , Fe 3+ , Ca 2+ , K + , Mg 2+ , Mn 2+ , Na + , Ni 2+ , Cd 2+ , Zn 2+ , Al 3+ , Dy 3+ , Er 3+ , Gd 2+ , Ho 3+ , La 3+ , Lu 3+ , Pr 3+ , Sm 3+ , Tm 3+ ) by a turn-off fluorescence response in 10 mM HEPES buffer (pH 6.3). The effects of anions such as S 2− , NO 3 – , SO 4 2− CO 3 2– , HCOO – , antioxidant ascorbic acid and 4-nitrophenyl acetate on the selectivity for UO 2 2+ detection were also studies. D-P5 sensor could be used for detecting UO 2 2+ in a good linear relationship with concentration in the range of 0–8.0 μM with a low limit of detection of 83.2 nM. Furthermore, the interaction of the sensor with UO 2 2+ was characterized by ESI-MS, IR, XPS and ITC measurements. The 1:1 binding stoichiometry between the sensor and UO 2 2+ was measured by the job’s plot and further verified by ESI-MS. The binding constant of the sensor with UO 2 2+ was calculated to be 9.8 × 10 4 M −1 by modified Benesi-Hildebrand equation. ITC results showed that the ΔH θ and ΔS θ for the interaction of D-P5 with UO 2 2+ were −(7.167 ± 1.25) kJ·mol −1 and 66.5 J·mol −1 ·K −1 , respectively. Time-resolved fluorescence spectroscopy indicated that the mechanism of fluorescence quenching of D-P5 by UO 2 2+ ion was static quenching process. In addition, this sensor displayed a good practicality for UO 2 2+ detection in lake water sample without tedious sample pretreatment.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2023
·Vol. 11
·期 14
·4930-4937
·
IF 6.6
Birong Fan,Wan Peng,Yu Zhang,Pingsheng Liu,Jian Shen
Photodynamic therapy (PDT) is becoming an efficient antibacterial strategy without drug-resistance. Here, we report a promising reactive oxygen species (ROS) conversion strategy to increase the antibacterial efficiency of an Eosin Y (EOS)-based PDT system. Based on visible-light illumination, EOS generates a high concentration of singlet oxygen (1O2) in the solution. With the introduction of HEPES in the EOS system, it can almost completely convert 1O2 to hydrogen peroxide (H2O2). The orders-of-magnitude increases in the half-lives of the ROS (H2O2vs.1O2) present in the solution can enable more persistent oxidation ability. Thus, it is able to increase the bactericidal efficiency (against S. aureus) from 37.9% to 99.9%, promote the inactivation efficiency of methicillin-resistant S. aureus (MRSA) from 26.9% to 99.4%, and enhance the eradication rate of MRSA biofilm from 69% to 90%. Further in vivo investigation showed that the increased oxidation ability of the EOS/HEPES PDT system can enable quicker healing and maturing (even better than that for vancomycin administration) of MRSA-infected skin wounds on rats. This strategy may find many creative applications for the efficient eradication of bacteria and other pathogenic microorganisms.
研究领域:
药理学
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微生物学
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生物医学工程
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传染病学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Shengliang Zhang,Hongyun Lu,Hanghang Lou,Ying Shi,Donghong Liu,Qihe Chen
The large-scale production of cultured meat has been restricted by high cost of serum, batch difference and unclear composition, thus it is important to develop serum-free media for cell growth. Here we obtained a serum-free medium (SFM), which was suitable for ex vivo culturing of myoblasts from larimichthys crocea . It offered simple composition and high effectiveness that supporting myoblast attachment, expansion and differentiation. Our findings revealed that the optimal SFM consisted of DMEM/F12 base medium, 5 mg/mL bovine serum albumin (BSA), 20 mmol HEPES, 1 % penicillin/streptomycin mixture (PS) and the exogenous additive hydrocortisone. The living cells of SFM could occupy about 65 % of the serum-containing medium (SM). Additionally, differentiation assays showed that myoblasts cultured without serum still differentiated into more muscle tubes, with a higher fusion index than those cultured with serum. Finally, transcriptome sequencing was conducted to demonstrate that hydrocortisone might act as a ligand binding to G protein-coupled receptors, triggering the downstream PI3K/AKT signaling pathway and leading to cell proliferation. Our findings will provide a basis for large-scale cell expansion and cell culture meat production in the future.
研究领域:
细胞生物学
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组织工程
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生物技术
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食品科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 7
·943-950
·
IF 2.596
Dandan Li,Lihua Gong,Zhenyang Qiao,Congcong Zhu,Qiaojuan Jia,Huajie Kong,Shuyan Jiao
A novel highly selective colorimetric fluorescence probe, POPH, has been successfully synthesized and it shows remarkable selective and sensitive response to Cu2+ in aqueous media (HEPES buffer, pH = 7.4). The fluorescence of probe POPH immediately decreased when 1.1 equiv. of Cu2+ was added to a solution of probe POPH, accompanied by color change from wathet blue to colorless under 365 nm UV lamp illumination. The possible bonding model of probe POPH with Cu2+ was examined through HRMS, FESEM, TEM, FTIR spectroscopy and DFT calculations. Moreover, the probe POPH could be used for monitoring Cu2+ in living cells using laser scanning confocal microscopy.
研究领域:
分析化学
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材料科学
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生物化学
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理论化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）