FOOD CHEMISTRY
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2017
·
IF 4.529
Yuyu Jia,Lin Wang,Li Ma,Zhaoguang Yang
A comparative study between microwave assisted and sonication methods was carried out to extract arsenic (As) species in shellfish samples using different extractants. Six As species including arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB) and arsenocholine (AsC) were simultaneously separated and determined by the HPLC–ICP-MS method. The microwave assisted method exhibited higher efficiency than sonication, especially using diluted HNO 3 as extractant. By compromising extraction efficiency, pretreatment time and stability of As species, the microwave assisted method using 1% HNO 3 at 100 °C for 1.5 h was selected to extract As from real samples. The proposed method has been applied to extract and determine As species in shellfish samples. The result of correlation analysis indicated that the proportion of AsB in the shellfish samples was decreased with total As concentration increasing due to the biotransformation threshold from inorganic As to AsB.
研究领域:
环境科学
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分析化学
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食品安全
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2019
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IF 3.206
Lanyun Fang,Yumei Zhang,Beibei Lu,Li Wang,Xunping Yao,Ting Ge
The present paper proposed an analytical method for speciation analysis of inorganic antimony in inhalable particulate matter, PM2.5, using high performance liquid chromatography coupling with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). A new extraction method consisted of reducing extraction and oxidizing extraction using EDTA as the key component in the extractant has obtained better extraction yield and preserves stability of both species during the extraction process. Stability of Sb species in EDTA-saturated nitric or tartric acid solution and in aqueous solution were carefully investigated. Using the optimized conditions, the method allows for the determination of Sb(III) and Sb(V) in PM2.5 samples with limits of 0.10 and 0.06 ng m −3 , respectively. The precision, expressed as relative standard deviation (RSD), was always lower than 8.5% and 6.6% for Sb(III) and Sb(V), respectively. The spiked recovery rates of Sb species at three different concentration levels were in the range of 90.0–110.0%. The extraction yield of the sequential extraction process confirmed by analysis of a certified reference material of urban particulate matter, NIST1648a, furnished by National Institute of Standards and Technology was 60%. This method was applied to determine Sb(III) and Sb(V) in PM2.5 samples collected in urban area in Ningbo City, China. The results showed that both Sb(V) and Sb(III) were found in most of the samples analyzed, Sb(V) being the main one, which represents 69–87% of the total Sb extracted in the considered samples, whereas Sb(III) represents the remaining 13–31%.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
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2017
·Vol. 32
·期 6
·1186-1195
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IF 3.379
Shan Li,Beibei Chen,Man He,Bin Hu
Protein phosphorylation analysis is important for understanding cell regulations. Mass spectrometry (MS) is a powerful technique for peptide phosphorylation analysis. However, quantification of low abundance phosphoproteins in a complex mixture of proteins is still a challenge. Here we report the development of hollow fiber (HF) supported TiO2 monolithic microextraction combined with capillary high performance liquid chromatography (capHPLC)-inductively coupled plasma-collision reaction cell (ICP-CRC)-MS for the absolute quantification of phosphopeptides. The membrane pores (∼200 nm) of the HF effectively carry more TiO2 on its surface to enhance the adherence of the TiO2 monolith with the HF. By using β-casein as a model phosphorylated protein, we optimized the HF-supported TiO2 monolithic microextraction towards phosphopeptides. Under the optimal conditions, a 100-fold enrichment factor was obtained and the monoliths can be reused 40 times. By monitoring 31P16O with O2 as a reaction gas in the CRC, polyatomic mass interference at m/z 31 is avoided and a low detection limit (2.9 nM) of phosphorus is achieved. The method of HF-supported TiO2 monolithic microextraction-capHPLC-ICP-MS provided the detection limit for phosphopeptides at the nM level. The proposed method was applied to the quantification of phosphopeptides in milk and milk powder samples.
研究领域:
蛋白质组学
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分析化学
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生物化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
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IF 5.7
Feng Guo,Pingxiu Zeng,Jinhua Liu,Hongmei Hu,Wenbin Zhu,Yuanchao Wang,Heyong Cheng
Background Tin and lead contamination is a global threat to marine ecosystems considering their species-specific toxicity, bioavailability and mobility. Hence simultaneous measurement of multiple tin and lead compounds at μg L −1 to pg L −1 levels in environmental water is always an indispensable but challengeable task. High performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) is one of the most widely used choices for this purpose because of good sensitivity, strong separation power and good compatibility. Previous HPLC-ICP-MS methods based on a single elemental speciation strategy are low-efficiency and sensitivity-insufficient for a large set of unstable samples and interaction of multiple metal(loid)s down to ng L −1 levels. Results In this study, we developed a sensitive, efficient and environment-friendly analytical method for accurate quantification of inorganic and organic species of tin and lead simultaneously based on HPLC-ICP-MS with online integration of solid phase extraction (SPE). By using graphene oxide modified silica conditioned with 1 mM benzoic acid to enrich tin and lead species from 10 mL sample, detection limits were improved to 2–8 pg per liter due to satisfactory enrichment factors (522−2848 folds). The SPE-HPLC-ICP-MS method was applicable to quantification of ultra-trace tin and lead species at pg L −1 levels in uncontaminated seawater . Tributyltin was the only tin species detected at subnanograms per liter levels while Pb(II) was the only lead species detected at several nanograms per liter in thirteen coastal seawater samples collected in Hangzhou Bay, indicating light contamination of tin and lead. Significance Overall, the proposed SPE-HPLC-ICP-MS method is highly sensitive, efficient and environment-friendly that are fairly suitable to routine speciation analysis of tin and lead in environmental, food, and biological samples.
研究领域:
环境科学
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分析化学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
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2019
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IF 1.267
Yue Yang,Zihui Liu,Hao Chen,Shengqing Li
A simple and fast method of low-density solvent based dispersive liquid-liquid microextraction (LDS-DLLME) followed by high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) has been developed for the speciation analysis of organoarsenic and inorganic arsenic in water samples. The low-density solvent (octanol) was given as the organic phase and injected into the aqueous sample (donor phase) with methanol as the disperser. With As (V), As (III), p-APAA, 4-HPAA, ROX and PAA as target species, factors of LDS-DLLME including pH value, anionic carriers, elution conditions and extractant, were studied in detail. Besides, volumes of solvents were further optimised by response surface methodology. Under the optimal conditions, the limits of detection for four phenylarsenics and arsenate were in the range of 0.001–0.039 μg L−1. The relative standard deviations (RSDs) were 3.6–9.4% and the enrichment factors varied from 6.2 to 70.8. The proposed method of LDS-DLLME-HPLC-ICP-MS was satisfactorily applied to the determination of six arsenic compounds in water samples with recoveries of 81.8–111.7% for the spiked lake water samples.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
ANALYTICAL LETTERS
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2019
·
IF 1.248
Zhipeng Wang,Jie Xu,Yanjun Liu,Zhaojie Li,Yong Xue,Yuming Wang,Changhu Xue
This study aimed at developing an accurate method for determining the levels of total arsenic (As) and arsenic species in edible shrimp with the collision/reaction cell (CRC) in the O2 mass-shift mode to remove interferences. The microwave-assisted extraction method demonstrated satisfactory efficiency (86.9%), and As species were separated rapidly within 11 min when the optimized chromatographic conditions were adopted. During the study, the limits of detection, precision, and spike recovery for six arsenic species were in the ranges from 0.010 to 0.028 μg/L, 1.89 to 3.14%, and 96.9 to 103.2%, respectively. The analytical methods were successfully applied to determine the concentrations of total arsenic and As species in eight shrimp samples. The results showed that arsenobetaine was the predominant As species, and inorganic arsenic contents were below 0.5 mg/kg in the shrimp samples analyzed. The health risk assessment indicated that the shrimp samples detected posed no particular risk of As to local consumers.
研究领域:
分析化学
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食品安全
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环境科学
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DOI
高影响因子
Toxics
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2024
·Vol. 12
·期 5
·325
·
IF 3.9
Ge Song,Honglian Tan,Chuhan Cheng,Peng Li,Xinyang Sun,Yuling Zhou,Yong Fang
Hexavalent chromium (Cr(VI)) is known as the most hazardous species of chromium. Speciation analysis of Cr in foods is of a great significance for assessing its influences on human health. In this study, a fast HPLC-ICP-MS method for the determination of Cr(VI) was developed for determining the content of Cr(VI) and also investigating its transformation in foods. The developed method employs an alkali extraction and weak anion-exchange column separation for distinguishing the Cr species, facilitating accurate Cr(VI) quantification within 1.5 min. This technique was applied to determine the Cr(VI) levels in a range of food products, including yoghurt, milk powder, rice flour, orange juice, green tea, white vinegar, and whole wheat bread. The results showed that no Cr(VI) was detected in these food products. Spiking experiments revealed that the recovery rate of Cr(VI) decreased with the increase in its contact time with food products. A further exploration of Cr(VI) in various food components such as vitamin C, tea polyphenols, whey proteins, gelatin, fructose, and cellulose indicated the conversion of Cr(VI) to organic Cr(III) over a period from 20 min to 60 h. It was found that high temperatures and acidic conditions accelerated the rate of Cr(VI) conversion to organic Cr(III) in the six food components mentioned above. This evidence suggests that natural reducing substances in foods probably prevent the occurrence of Cr(VI).
研究领域:
分析化学
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食品科学
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环境健康
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2025
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IF 1.3
Kewei Wang,Xilong Qian,Ying Zhang,Zheng Liu,Weihua Zhang,Liu Zhou,Yanqiong Pan,Liwen Zheng,Fang Fang,Yang Zhang,Yehuang Wang,Bin Jiang,Shengjin Liu
Yuhuang Shengji ointment (YSO) is a traditional Chinese medicine clinical prescription from Professor Wang Yehuang, a famous Chinese medicine professor in Jiangsu Province China.It is used for the treatment of postoperative healing of anal fistula in clinic practice. It is composed of Corydalis Rhizoma, Radix Sanguisorbae Carbonisata, Natrii Sulfas, Alumen Ustum, Plant Soot and Borneolum. It has the effects of eliminating dampness, astringing sores, hemostasis, pain relief, detumescence and muscle regeneration. According to the characteristics that YSO mainly contains inorganic, organic and volatile components, the comprehensive quality control of the ointment was achieved by different analytical methods. The organic components in the ointment were qualitatively analyzed by UPLC-Q-TOF-MS to determine the main chemical components. HPLC fingerprint of the organic components of the ointment was further established. ICP-MS technology is used to analyze the inorganic elements in ointment, which provides data support for the quality control of inorganic components. The Flash GC e-nose is used for rapid detection of volatile substances in ointment, and the fingerprint of volatile components is established. The comprehensive use of these analytical techniques not only reflects the overall chemical characteristics of the ointment, but also helps to evaluate the consistency between different batches of products. This study successfully established a comprehensive quality analysis method, which provided a basis for the quality control of YSO. At the same time, it provides a more comprehensive quality control strategy for ointment which mainly contains organic, inorganic and volatile components.
研究领域:
中药学
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分析化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
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IF 5.7
Xiaolai Zhang,Fangli Li,Jingbo Chao,Zhicai Li,Guimin Zhang,Lihai Zhai,Ligang Hu,Heping Jiao,Zhenhua Wang
Background Trace levels of organic and inorganic lead and mercury species in the environment, including divalent lead (Pb 2+ ), trimethyllead (TML), divalent mercury (Hg 2+ ), monomethylmercury (MeHg), and ethylmercury (EtHg), are highly toxic to humans and ecology. It is of great importance for speciation of lead and mercury to evaluate the toxicity of lead and mercury and their biogeochemistry in the environment. However, simultaneous multi-elemental enrichment and speciation at trace level remains a challenge. There are few reports of simultaneous magnetic solid-phase extraction (MSPE) of organic and inorganic lead and mercury species at trace level in the real water. Results In this work, a novel core-shell magnetic hydrazine-linked covalent organic frameworks (Fe 3 O 4 @COF-TCH) was prepared for the first time by grafting hydrazine-linked COFs on the Fe 3 O 4 nanoparticles. Fe 3 O 4 @COF-TCH with abundant thione and imino groups has strong adsorption for lead and mercury species. Based on it, a simple and practical magnetic solid-phase extraction high-performance liquid chromatography-inductively coupled plasma mass spectrometry (MSPE-HPLC-ICP-MS) method was developed for extraction and determination of trace lead and mercury species, including Hg 2+ , MeHg, EtHg, Pb 2+ and TML. The limits of detection (3δ) of the developed method were 0.08, 0.81, 0.90, 0.56 and 0.88 ng L −1 with the enrichment factors (EFs) of 384, 376, 379, 389 and 360-fold for Pb 2+ , TML, Hg 2+ , MeHg and EtHg, respectively. The high accuracy and reproducibility have been proved by the spiked recoveries (94.4–103 %) in real samples. Significance The proposed method with simple operation and high sensitivity has been successfully applied to simultaneous speciation of lead and mercury at trace levels in the water samples with complicated matrices, including underground water, surface water, sea water. Meanwhile, it has the advantages of cost-saving, labor-saving and time-saving and is suitable for the investigation and risk assessment in water. The development of MSPE-HPLC-ICP-MS method provides ideas and guidance for the simultaneous multi-elemental enrichment and speciation.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Shiwei Yang,Danyu Zhang,Heyong Cheng,Yuanchao Wang,Jinhua Liu
For the past several decades, lots of adsorbent nanomaterials have been adopted for the enrichment of mercuric compounds. In this paper, graphene oxide bounded silica particles were employed as the absorbent for online preconcentration of Hg(II), methylmercury (MeHg) and ethylmercury (EtHg) by solid phase extraction . The adsorbent offered satisfactory adsorption capacities of 40.7 for Hg(II), 91.4 for MeHg and 103.8 mg g −1 for EtHg. Enrichment conditions such as type, concentration and volume of conditioning and eluting reagents were optimized. The pH, volume and flow rate of sample were also optimized. High enrichment factors (1963, 1881 and 1794 for Hg(II), MeHg and EtHg, respectively) were obtained with 10 mL sample (adjusted to pH 4–7) at 10 mL min −1 flow rate, 5 μL of 10 mM benzoic acid for elution and 4 mL of 1 mM 4-phenyl-3-aminothiourea for preconditioning. The detection limits obtained were downscaled to be 0.005 for Hg(II), 0.006 for MeHg and 0.009 ng L −1 for EtHg with relative standard deviations of all the analytes below 5%. Standard reference material and spiked water samples were analyzed for validating the method with satisfactory recoveries of 89–106%. The present method was successfully applied to determine mercury compounds in drinking water , and river and tap water , in which Hg(II), MeHg and EtHg were presented below the China's drinking standard limit of 1 μg L −1 .
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）