SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Qian Liu,Fang Wu,Huixia Di,Yingna Bi,Meng Meng,Dingbin Liu,Yongmei Yin,Rimo Xi
Developing highly sensitive and specific method is essential for trace Hg 2+ quantifying. Herein, we established a background-free surface-enhanced Raman scattering (SERS) platform which allows not only the detection of Hg 2+ in homogeneous samples, but also high-precision profiling of Hg 2+ distribution in zebrafish. Its superior anti-interference capability is due to the introduction of a high signal-to-noise-ratio (SNR) Raman reporter with an acetylenyl group, which present single nonoverlapping peak at 2125 cm −1 , avoiding interference from other substances. Additionally, the specific recognition of Hg 2+ -bis-thymine complexes and the signal amplified hybridization chain reaction (HCR) were effectively utilized in this design. Benefited from the above strategies, a detection range for Hg 2+ from 1.0 fM to 100 pM was achieved with a detection limit of 0.2 fM. Additionally, Raman imaging was first performed in zebrafish sections to study Hg 2+ distribution which provided a reference for the detection of Hg 2+ pollution in fish. This approach could be extended to target other interested analytes both in simple and biological samples for various purposes.
研究领域:
分析化学
·
环境科学
·
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Lu Tian,Wenjing Zhao,Lin Li,Yaoli Tong,Guanlin Peng,Yingqi Li
We herein provide a facile and green strategy for the fabrication of red fluorescence gold nanoclusters ( [email protected] ) with low toxicity, good stability and excellent fluorescence (λ em = 650 nm, λ ex = 320 nm) by mixing aqueous tetrachloroauric acid with diluted egg white under alkaline environment using microwave-assisted method. Confocal fluorescence imaging showed that the [email protected] can enter living cells and distribute in the cytoplasm. In addition, the fluorescence of [email protected] is sensitive to the response of Cu 2+ and Hg 2+ with a detection limit of 14.48 nM and 0.81 nM, respectively. Interestingly, we found that the fluorescent signal of the quenched [email protected] (II) system could be recovered in the presence of the glutathione (GSH) in Tris-HCl medium (pH 6.5). This assay displayed a rapid response and highly selectivity toward GSH with a detection limit of 91.9 nM. More excitingly, the fluorescent signal of the [email protected] (II) system (OFF) could be activated in living tumor cells (ON) such as HepG2 cells rather than in living normal cells (HT22 cells, OFF) without any exogenous GSH. The result showed that the system can be successfully applied for tumor cells recognition, which implied its great potential application in early prevention of cancer. Furthermore, the [email protected] can also be used for patterning, staining and temperature sensing. We propose that the highly fluorescent [email protected] can be eco-friendly and potentially large-scale development for versatile applications and so merits further study.
研究领域:
纳米技术
·
生物医学工程
·
癌症研究
·
细胞生物学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Xiaotong Wu,Lei Tan,Yuling Li,Wanqiong Liu,Zhihua Peng,Yanshan Dong,Zeyu Huang,Lin Zhang,Yong Liang
In this work, we demonstrated a sensor array with multiple fluorescence channels using dendritic mesoporous silica nanoparticles embedded with three quantum dots for the determination of four heavy metal ions (Hg 2+ , Cu 2+ , Cr 3+ , and Ag + ). Carboxyl-modified CdTe QDs with three different fluorescence emission wavelengths were loaded onto a dendritic mesoporous supporter by an amidation reaction. The fluorescence sensor array exhibited excellent analytical performance for discrimination and semi-quantification of heavy metal ions from a single test, which simplified detection procedures. The four heavy metal ions exhibited different degrees of quenching of the fluorescence emission intensities of the three quantum dots and resulted in a variant data matrix for linear discriminant analysis . Under optimized conditions, the fluorescence sensor array discriminated the four heavy metal ions in a concentration range of 0.05–5 μmol/L, and semi-quantified Hg 2+ , Cu 2+ , Cr 3+ , and Ag + with a limit of detection of 2.51 nmol/L, 5.15 nmol/L, 3.81 nmol/L, and 5.74 nmol/L, respectively. The fluorescence sensor array integrated the sensing units into a single nanoparticle instead of the complex multiple detection steps used in traditional sensor arrays, providing an alternative strategy for constructing a single-well sensing platform. Furthermore, the fluorescence sensor array showed great practical potential for distinguishing heavy metal ions in raw water and crayfish samples.
研究领域:
分析化学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Ning Wu,Hao Guo,Rui Xue,Mingyue Wang,Yujuan Cao,Xiaoqiong Wang,Mengni Xu,Wu Yang
Mercury (Hg 2+ ) is a highly hazardous and widespread environmental pollutant with bioaccumulative properties, so highly sensitive detection and effective removal of mercury in water environment are very necessary. Considering “soft base” property of N atom, N-containing organic groups cannot coordinate effectively with “hard acid” rare earth ions . A [Sm(ATPA) 1.5 (DEF)] n (Sm-MOF) (H 2 ATPA = 2-aminoterephthalic acid) containing free amino groups is constructed for simultaneous detection and removal of Hg 2+ ions. Hg 2+ can quench effectively the charge transfer emission of Sm-MOF in aqueous solution, and simultaneously induce the Sm 3+ characteristic fluorescence emission of Sm-MOF. The results shows that the prepared Sm-MOF has an excellent selectivity and a high sensitivity for detecting Hg 2+ with a wide linear range of 0.6–300 μM and a low detection limit of 0.87 μM. At the same time, the MOF is also employed to adsorb and remove Hg 2+ in aqueous solution and it is found that over 97% of Hg 2+ can be removed in approximately 2 h.
研究领域:
环境科学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）