Discover Nano
·
2023
·Vol. 18
·期 1
·1-11
·
IF 0
Zhu Xiaofeng,Cheng Kangning,Ding Yue,Liu Huanqing,Xie Shuqi,Cao Yuwei,Yue Weiwei
Herein, we have constructed a magnetic graphene field-effect transistor biosensor (MGFETs) for highly sensitive detection of cardiac troponin I (CTNI). Graphene films transferred to ITO conductive glass as conductive channels. CTNI aptamer was immobilized onto the graphene film via 1-pyrene-butanoic acid succinimidyl ester (PBASE) to capture CTNI. Magnetic nanobeads (MBs) modified with CTNI antibody were added to the reaction chamber to form an aptamer/CTNI/antibody/magnetic nanobeads sandwich-type complex. We found that the magnetic force exerted on the complex leads to an impedance change of the graphene film. The reason for this result is that the magnetic field exerts an influence on the MBs, causing CTNI aptamer strand to bend, resulting in a change in the distance between the double conductive layers of the graphene film surface and the test solution. With periodic sampling integration, different concentrations of CTNI can be detected with high sensitivity. Due to the stringent recognition capability and high affinity between the CTNI aptamer and CTNI, MGFETs have the potential to detect various types of proteins. Furthermore, MGFETs also have the potential to be utilized for the detection of DNA or specific cells in the future.
研究领域:
生物传感器
·
纳米技术
·
生物医学工程
·
分析化学
·
心脏病学
✓ 已验证
DOI
CRYSTAL GROWTH & DESIGN
·
2024
·
IF 3.2
Shuhong Song,Lei Wang,Yaqian Qu,Huimin Li,Hongshuai Wang,Peizhuo Han,Zeliang Gao,Xutang Tao
The crystal growth mechanism of form I (AZ-I) of the antihypertensive drug azilsartan is revealed by in situ atomic force microscopy (AFM). On the dominant (100) face, AZ-I grows by a classical spiral growth mechanism. The growth spiral is asymmetric due to the different step kinetics in different crystallographic directions. The normal growth rate of the (100) face is inferred to be much lower than the step velocity within this face according to the step geometry, resulting in the plate-like crystal morphology. The dependence of step velocity on terrace width indicates that molecules incorporate into steps primarily by a surface diffusion mechanism involving molecular adsorption on terraces at first and then diffusion into steps. Within the (100) face, steps in different directions exhibit different step edge free energies and total minimal energies for step advancement. The step kinetics depends on the step length within a certain range. These findings provide a fundamental understanding of drug crystal growth at the microscale.
研究领域:
药物结晶学
·
材料科学
·
纳米成像
·
药物研发
·
物理化学
✓ 已验证
DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2015
·Vol. 31
·期 4
·585-589
·
IF 0.875
Wei Qi,Wang Liyuan
Molecular glass resist has been considered as one of the best choices for a new generation of lithography. In this work, a new type of photoactive compound was obtained by the esterification of tannic acid with 2-diazo-1-naphthoquinone-4-sulfonyl chloride(2,1,4-DNQ-Cl) and ditertbutyl dicarbonate. The new obtained compound possessed both a photosensitive group of diazonaphthoquinone sulfonate(2,1,4-DNQ) and a group of acidolytic protection. Upon the irradiation of the compound under 365 nm light, the former group was photolyzed and converted into indene carboxylic acid along with a small amount of sulfonic acid, which could lead to the deprotection of the latter group. As a result, a novel i-line molecular glass photoresist was formed with the chemical modification of tannic acid. The experimental results show that the modificated compound had a fair solubility in many organic solvents. The lithographic performance of the resist was evaluated on an i-line exposure system with high photosensitivity and resolution as well.
研究领域:
材料科学
·
微纳加工
·
高分子化学
·
光电子学
·
半导体工艺
✓ 已验证
DOI
高影响因子
MedComm
·
2024
·Vol. 5
·期 12
·e70033
·
IF 10.7
Jing Li,Mimi Chen,Dingwen Huang,Ziyin Li,Yu Chen,Jinhua Huang,Yuanqun Chen,Zhili Zhou,Zhiying Yu
Ovarian cancer (OV) ranks among the deadliest gynecological cancer, known for its high risk of relapse and metastasis, and a general resistance to conventional platinum‐based chemotherapy. Selenoprotein I (SELENOI) is a crucial mediator implicated in human hereditary spastic paraplegia. However, its role in human tumors remains poorly elucidated. Here, we comprehensively analyzed SELENOI expression patterns, functions, and clinical implications across various malignancies through the integration of bulk transcriptomics, cancer databases, and in vitro and in vivo experiments. Pan‐cancer analysis indicated upregulated SELENOI expression across various cancers, correlating with augmented malignancy, suppressed tumor immunity and poor prognosis. Knockdown of SELENOI caused G0/G1‐phase cell cycle arrest and diminished aggressive cancer phenotypes in OV cells. Moreover, SELENOI inhibition augments ferroptosis and reverses the cisplatin resistance in OV cells by modulating Akt phosphorylation. Conversely, overexpression of SELENOI in OV cells enhanced therapeutic sensitivity to cisplatin by upregulating Akt phosphorylation. Importantly, in vivo studies demonstrated that SELENOI inhibition suppressed ovarian tumor growth and enhanced cisplatin's anticancer effects. These findings highlight the significant role of SELENOI in OV by modulating ferroptosis and chemotherapy resistance. Targeting SELENOI represents a promising therapeutic approach to promote the efficacy of platinum‐based chemotherapy in OV, particularly in cases of resistance. Graphic summary of targeting SELENOI for promoting ferroptosis and reversing chemoresistance in ovarian cancer. Inhibition of SELENOI activated unsaturated fatty acid synthesis and impaired Akt phosphorylation, resulting in the upregulation of ACSL4 expression and induction of ferroptosis. Reduced Akt phosphorylation also led to the downregulation of ALDH3A1 expression, a key gene associated with cisplatin‐resistance, thereby enhancing cisplatin cytotoxicity in SELENOI‐silenced ovarian cancer cells.
研究领域:
肿瘤学
·
分子生物学
·
癌症治疗
·
转化医学
✓ 已验证
DOI
PubMed
高影响因子
Materials Today Chemistry
·
2024
·
IF 6.7
Yuan Tang,Pengbo Lyu,Desheng Yin,Zhaoqi Liu,Nian Pan,Kai Li,Ying Ye,Changfu Xu,Lizhong Sun
Ultra-broadband near-infrared (NIR) lighting sources covering NIR Ⅰ to III regions are pivotal components for NIR spectroscopy devices. However, achieving ultra-broadband and efficient emission covering the entire NIR Ⅰ to III regions remains a significant challenge. To address this, we selected Mg 7 Ga 2 GeO 12 as host material to realize ultra-broadband NIR emission. Density functional theory calculations on formation energies confirmed the feasibility of doping both Ni 2+ and Cr 3+ within Mg 7 Ga 2 GeO 12 . Band structure calculations reveal a significantly reduced band gap upon the doping of Ni 2+ . Following that, we synthesized Mg 7 Ga 2 GeO 12 :Ni 2+ through conventional solid-state-reaction method. This phosphor exhibits broadband NIR emission with a full-width-at-half-maximum of 377 nm in the range of NIR II ∼ III (1100–2100 nm). To further enhance the emission efficiency, we co-doped Mg 7 Ga 2 GeO 12 :Ni 2+ phosphor with Cr 3+ . This co-doping strategy not only dramatically enhances the Ni 2+ emission intensity, but also extends the lower limit of emission band to 600 nm. However, a spectral gap around 1150 nm remains between Cr 3+ and Ni 2+ emission centers. To bridge this spectral gap, we employed LiScSiO 4 :Cr 3+ phosphor alongside Mg 7 Ga 2 GeO 12 :Ni 2+ ,Cr 3+ phosphor to fabricate a phosphor converted LED (pc-LED) with ultra-broadband NIR emission covering entire NIR Ⅰ to III regions. By utilizing this pc-LED as the lighting source for NIR spectral detection, multiple organic functional group signals can be identified simultaneously in NIR II and III bands from 1000 to 2100 nm. The performance of such an NIR pc-LED not only confirms the application potential of Mg 7 Ga 2 GeO 12 :Ni 2+ ,Cr 3+ phosphor in non-destructive spectral analysis but also underscores the feasibility of combining two phosphors to achieve ultra-broadband NIR pc-LEDs, rendering them suitable to be lighting sources for portable spectrometers.
研究领域:
材料科学
·
光学
·
光谱学
·
光子学
·
化学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 35
·29638-29645
·
IF 3.289
Xin Ge,Xinzhi Chen,Chao Qian,Shaodong Zhou
Ullmann type C–N coupling reaction catalyzed by copper(I) with D-glucosamine derivatives as promoters was studied by means of combined experimental/theoretical investigation. The catalytic role of D-glucosamine was addressed. In contrast with previous speculations, the amino group may not count in the catalytic cycle in which the oxidative addition/reductive elimination mechanism works. Experimental results are in good agreement with theoretical findings. Extensive work indicates the wide applicability of the C–N coupling strategy exploited in this work.
研究领域:
有机化学
·
催化化学
·
药物化学
·
计算化学
·
合成方法学
·
生物正交化学
·
金属有机化学
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·Vol. 416
·期 10
·2541-2551
·
IF 3.8
Niu Yanyan,Chen Yuxue,Zhou Juan,Sun Wei
In this study, an online electrochemistry coupling high-performance liquid chromatography-mass spectrometry (EC-HPLC-MS) technology has been developed for simulating metabolic reactions and rapid analysis of metabolites of flavone, quercetin, and rutin, which are not only widely present compounds with pharmacological activity in nature, but also have structural similarity and variability. The simulated metabolic processes of the substrates (phase I and phase II metabolism) were implemented on the surface of glassy carbon electrode (GCE) by using different electrochemical methods. After online chromatographic separation, the products were transmitted to a mass spectrometer for detection, in order to speculate relevant reaction pathways and structural information of the reaction product. The main metabolites, including methylation, hydroxylation, hydrolysis, and conjugation reaction products, had been successfully identified through the designed in situ hyphenated technique. Furthermore, compared with metabolites produced by in vitro incubation of rat liver microsomes, it was found that the products of electrochemical simulated metabolism were more abundant with diverse metabolic pathways. The results indicated that the proposed method exhibited advantages in the sample pretreatment process and detection cycle without compromising the reliability and accuracy of the results. Graphical
研究领域:
分析化学
·
药物化学
·
电化学
·
代谢组学
·
生物分析化学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Huidan Li,Ye Liu,Jun Ding
Abstract Isothiocyanates (ITCs) are bioactive hydrolysis products of glucosinolates in Brassicaceae plants with diverse health-promoting properties. However, comprehensive profiling of ITCs remains challenging due to the limited availability of authentic standards, the underexplored structural diversity of naturally occurring ITCs, and poor mass spectrometry (MS) detection sensitivity. Here, we established a N,N-dimethylethylenediamine (DMEDA) derivatization strategy and constructed an in silico MS/MS library for systematic ITC annotation. DMEDA derivatization markedly improved LC–MS detection sensitivity, enabling the reliable detection of low-abundance ITCs with limits of detection at the nanomolar level. Detailed characterization of the MS/MS fragmentation behaviors of derivatized ITC standards revealed subclass-dependent fragmentation patterns, which were subsequently translated into spectral prediction rules for the generation of an in silico mass spectral library with 2209 simulated ITC structures. Application of the developed workflow to Brassicaceae plant samples enabled the annotation of 50 endogenous ITCs, including 4 low-abundance compounds that were previously not reported. Our work provides a valuable resource for the systematic exploration of ITC structural diversity and facilitates functional studies of glucosinolate-derived bioactive metabolites.
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Huixiang Yan,Xiaodi Tang,Xudong Zhu,Yanbo Zeng,Xing Lu,Zhengzhi Yin,Yixia Lu,Yiwen Yang,Lei Li
Herein we report a sandwich-type electrochemical immunosensor for sensitive detection of cardiac troponin I (cTnI) using a new composite of carboxyl-terminated ionic liquid and helical carbon nanotubes (CIL-HCNTs) as platform and ferrocenecarboxylic acid (Fc-COOH) as signal label. The composite of CIL-HCNTs was prepared by a simple and convenient one-step ultrasound method and was then characterized by Fourier transform infrared spectroscopy, scanning electron microscope, cyclic voltammetry and chronocoulometry. CIL-HCNTs, possessing large surface area and high electrical conductivity, provide a good platform for anti-cTnI monoclonal antibody immobilization through the carboxyl groups of CIL-HCNTs. The Fc-COOH, as a signal label based on the oxidation process, was combined with the CIL-HCNT platform to construct the electrochemical immunosensor for cTnI determination. Under optimal conditions, the electrochemical immunosensor exhibited desirable performance for cTnI determination with a wide range of linearity from 0.01 to 60.0 ng mL −1 and a relatively low detection limit of 0.006 ng mL −1 . Importantly, the immunosensor showed excellent specificity and acceptable reproducibility as well as satisfactory reliability for human serum sample analysis.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Jiaqi Fu,Lianghua Xie,Zhen Zong,Khairiah M. Alwutayd,Hany A. El-Shemy,Wei Chen
Abstract Cyanidin-3-O-rhamnoside (C3R) and cyanidin-3-O-glucoside (C3G) show strong anti-inflammatory and antioxidant activities with great potential for chronic disease prevention. However, C3R biosynthesis from (+)-catechin has not been reported, and C3G production from this substrate is low for industrial applications. Here, we constructed a microbial platform for the efficient C3R and C3G production via genetic engineering and metabolic optimization. We screened glucosyltransferases and rhamnosyltransferases from various plants to identify the optimal combinations for C3G and C3R biosynthesis. Tuning UDP-glucose and UDP-rhamnose balance by plasmid copy number increased the C3R concentration by 120%. Overexpression of MdtH and yadH improved extracellular C3R and C3G accumulation by 16% and 31%, respectively. After optimizing substrate and culture conditions, the engineered strains produced 529.01 mg/L of C3R and 926.47 mg/L of C3G, with C3R synthesis reported for the first time. This study underscores the key roles of glycosyltransferases and UDP-sugar modulation, providing a versatile strategy for producing other disaccharide- or rhamnose-containing natural products.
✓ 已验证
DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2026
·
IF 3.6
Bao Xiao,Hong-Tao Jiang,Wenzhuo Han,Di Sun,Ning Li,Yiru Tai,Yang-Yang Lv,Bin-Bin Zhang
Abstract Bismuth-based chalcohalides have garnered significant attention for optoelectronic applications due to their low toxicity and excellent photophysical properties. Herein, high-quality BiS1−xSexI (0 ≤ x ≤ 1) single crystals were successfully grown via physical vapor transport (PVT) method. Se element substitution not only tunes the bandgap from 1.53 eV to 1.29 eV but also reduces the resistivity by over 6 orders of magnitude, enabling distinct photodetection mechanisms. For the end-member BiSI (BiS1−xSexI, x = 0), the device operates in a bias-dependent photoconductive mode, achieving a high responsivity of 1 mA/W at 10 V bias under 425 nm illumination. Conversely, for Se-rich compositions like BiS1−xSexI (x = 0.75, and 1), the photothermoelectric (PTE) effect dominates, allowing for sensitive self-powered broadband detection. The optimized PTE device exhibits stable ON-OFF switching under 425 nm illumination, yielding a responsivity of 227 mV/W with a specific detectivity of 6.5 × 106 Jones. These results highlight the versatility of BiS1−xSexI crystals, offering a pathway to switch between high-gain photoconductors and broadband, self-powered PTE detectors through compositional engineering.
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2026
·
IF 7.6
Dongqi Fan,Zicheng Wang,Yuan Tian,Cheng Wang
Abstract The inherent Li dendrite growth, notorious shuttle effect, and severe redox reaction kinetics of lithium polysulfides (LiPSs) impede the feasibility of practical development of lithium–sulfur (Li–S) batteries. Herein, a bi-functional defect-rich TiO2–x/SrTiO3–x nanocube, embedded in a N-doped carbon (NC) freestanding fabric, was prepared and effectively used as a sulfur host and an interlayer between the separator and lithium anode for Li–S full batteries. The spontaneous formation of a built-in electric field (BIEF) between TiO2–x and SrTiO3–x guides self-driven charge rearrangement, resulting in strong directional LiPS migration to boost sulfur redox kinetics and fast, uniform Li+ transfer to avoid Li dendrite growth. Meanwhile, the introduction of oxygen vacancies can induce a ferroelectric phase and provide more active sites for lithium/sulfur storage. The whole 3D framework, enabled with fast electron/ion transport, reduces the Li nucleation overpotential and lowers the energy barrier for LiPS conversion. Consequently, Li||Li cells with the TiO2–x/SrTiO3–x@NC interlayer cycled stably over 600 h at 5 mA cm–2 and 5 mAh cm–2. Furthermore, Li–S full batteries delivered 1285 mAh g–1 at 0.1 C with merely 0.074% per-cycle capacity decay. Our findings emphasized the efficacy of a defect-rich TiO2–x/SrTiO3–x ferroelectric heterostructure implanted into a 3D conductive framework.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）