Nanoscale Advances
·
2019
·Vol. 1
·期 9
·3607-3613
·
IF 0
Zhaohong Su,Yongbing Cheng,Chaorong Li,Yuanfu Xiong,Langtao Xiao,Shu Chen,Xiaoli Qin
An amperometric immunosensor based on new thiolated bionanocomposite with a high dispersion of gold nanoparticles (AuNPs) for the sensitive detection of indole-3-acetic acid (IAA) is being reported herein. Briefly, a thiolated nanocomposite was prepared via the microwave-assisted thiol–ene reaction of 2,5-dimercapto-1,3,4-thiadiazole (DMcT) with oxidized polyaniline (PANI), which was synthesized in the presence of multiwalled carbon nanotubes (MWCNTs), yielding thiolated polyaniline (TPANI)-MWCNTs. Further, AuNPs were deposited on the TPANI-MWCNTs by microwave-assisted method to obtain a AuNPs/TPANI-MWCNTs nanocomposite. Finally, the thiolated bionanocomposite film was constructed via the specific chemical reaction between boronic acid functionalized AuNPs and the vicinal diol functionalized AuNP labeled immunoglobulin G (IgG–AuNPs). The change in the reduction peak current of Fe(CN)63− was used to monitor the immunoreaction between IAA and antibody. The TPANI-MWCNT nanocomposites uniformly disperse AuNPs, IgG–AuNPs and anti-IAA-AuNPs, leading to the amplification of the signal of the immunosensor. Fourier transform infrared spectra (FTIR), cyclic voltammetry (CV), transmission electron microscopy (TEM), ultraviolet visible spectroscopy (UV-vis) and differential pulse voltammetry (DPV) were used to characterize the nanocomposite film and the stepwise modification of the immunosensor. The prepared thiolated bionanocomposite material has good biocompatibility, a highly uniform dispersion of the AuNPs with a narrow size distribution as verified by TEM, and high load/activity of the immobilized antibody proved via DPV. The fabricated IAA amperometric immunosensor not only exhibits a good linear arrange from 1.0 pg mL−1 to 10 ng mL−1 with the limit of detection of 0.97 pg mL−1 (S/N = 3), but also possesses good selectivity, reproducibility and stability for the detection of IAA.
研究领域:
分析化学
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纳米技术
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生物传感器
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环境科学
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产品关联
DOI
PubMed
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Xiang-Yun Lin,Dong-Yang Tian,Zeng-Qiang Wu,Ning Bao
Electrochemical sensors based on molecularly imprinted polymer (MIP) provide an exceptional platform for sensitive and selective analysis of analytes in complex matrix through incubation while in vivo analysis is critical for achieving quantitative information for the biological study in real time. However, the deployment of MIP electrochemical sensors for in vivo analysis highly depends on their forms and fabrication. Here blade-style MIP electrochemical sensors were designed and fabricated in batches based on the technique of lamination, focusing on their applications for in vivo analysis of trace indole-3-acetic acid (IAA) in plants. The blade-style electrochemical sensors were fabricated using stainless steel sheets as supporting and conductive substrates for the working electrodes. The MIP sensors were prepared in batches using cyclic voltammetry (CV) based electropolymerization of pyrrole (the functional monomer) and IAA (the template) followed by elution under a constant potential at a multichannel electrochemical station with improved throughput. After optimization, the sensors could stably and reproducibly quantify IAA with the detection limit of 0.1 μmol·L −1 . The blade-style MIP sensors could be either plugged in tomato fruits for in vivo analysis of IAA or coupled with a piece of hydrogel for non-invasive analysis of IAA in tomato leaves. The blade-style MIP electrochemical sensors may provide a platform for in vivo analysis by not only obviating the time-consuming sample treatment but also simplifying the analytical procedure. This study may pave the way for extensive applications of MIP electrochemical sensors for real-time, spatially resolved analysis of endogenous biomolecules in living organisms in the near future.
研究领域:
分析化学
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植物生理学
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电化学
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生物传感器
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分子印迹技术
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化学生物学
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产品关联
DOI
高影响因子
HOLZFORSCHUNG
·
2016
·Vol. 71
·期 2
·179-188
·
IF 1.711
Min Yu,Kai Liu,Shengquan Liu,Haiyan Chen,Liang Zhou,Yamei Liu
Auxins as phytohormons, responsible for coordination of growing processes, also contribute to the formation of tension wood (TW) in trees, but the mechanism of this process is still unclear. In this study, it has been tried to induce TW formation in erect hybrid poplar trees (in opposite to inclined or bended trees) by treatment with exogenous indole-3-acetic acid (IAA, as one of the auxins) or N-1-naphthylphthalamic acid (NPA) as an auxin transport inhibitor. The treatment with IAA resulted in TW formation and, as expected, NPA treatment did not. The gelatinous fiber formation and higher cellulose content in the cell wall were unambiguous indicators for TW formation. Real-time polymerase chain reaction (PCR) analysis revealed that genes of PIN1 , ABCB1, and AUX2 involved in polar auxin transport were highly expressed in trees treated with exogenous IAA. Moreover, expressions of cellulose biosynthesis related genes of UGP1 , UGP2 and CesA13 were strongly up-regulated. These observations indicate that the accelerated intercellular polar auxin transport caused by exogenous IAA is accounted for TW formation, i.e. the signal transduction of auxin is affected, which then facilitates cellulose biosynthesis. In contrast, the transcript abundances of PIN1 and all selected cellulose synthases ( CesAs ) were decreased after NPA treatment via inhibiting the cellular auxin efflux with negative effects on plant’s primary growth. These results are interpreted that TW formation is closely associated with the acceleration of intercellular polar auxin transport.
研究领域:
植物学
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分子生物学
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林学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2024
·
IF 5.7
Yan Liu,Jiajia Han,Guosheng Chen,Siming Huang,Shuyao Huang,Juan Zheng,Jianqiao Xu,Fang Zhu,Gangfeng Ouyang
Phytohormones are a class of endogenous substances that separately or synergistically regulate the growth, development, and differentiation of plants. Accurately and efficiently detecting and monitoring the concentration of plant hormones in living plants is of significant importance. Herein, a novel mesoporous carbon hollow spheres (MCHS)-based in vivo solid phase microextraction (SPME) probe was designed for in vivo sampling of plant hormones. The designed MCHS features the advantages of high surface area, porous shells, and large hollow spaces, facilitating the dynamic adsorption and enrichment of target phytohormone. In addition, a cationic polyelectrolyte, (poly (diallyl dimethyl ammonium chloride) (PDDA), was further modified onto the MCHS to expedite the extraction process by electrostatic interaction. Utilizing the MCHS@PDDA probe in combination with HPLC-MS/MS facilitated the continuous monitoring of three plant hormones (abscisic acid (ABA), indole-3-acetic acid (IAA), and gibberellin (GA3)) in Chinese aloe. The detection limit of this method was 0.016–0.090 μg/L, the linear range was 10–1000 μg/L, and both the RSD of the single probe (n = 6) and probe-to-probe test (n = 6) were less than 7.2 %. This method had excellent accuracy and good reproducibility comparable to the traditional sample pretreatment method. Ultimately, this established in-vivo SPME method was successfully adopted to quantify three selected plant hormones in living Chinese Aloes, providing a new method for the long-term monitoring of endogenous active substances in living system.
研究领域:
分析化学
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植物科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
·
2021
·Vol. 21
·期 1
·1-12
·
IF 4.215
Wang Yu,Zhang Wenze,Liu Weikang,Ahammed Golam Jalal,Wen Wenxu,Guo Shirong,Shu Sheng,Sun Jin
Background Despite significant limitations of growth medium reuse, a large amount of organic substrate is reused in soilless cultivation of horticultural crops in China. Arbuscular mycorrhizal fungi (AMF) can promote nutrient absorption and improve plant tolerance to biotic and abiotic stresses. However, the mechanisms governing the effects of AMF on crop growth in organic continuous cropping substrates have not been elucidated.Results In this study, we showed that the inoculation of AMF in continuous cropping substrates promoted growth and root development, and increased the root and NADP-malic enzyme (NADP-ME) activity of tomato seedlings. Root transcriptome analysis demonstrated that the plant hormone signal transduction pathway was highly enriched, and 109 genes that positively correlated with the AMF-inoculated plant phenotype were obtained by gene set enrichment analysis (GSEA), which identified 9 genes related to indole acetic acid (IAA). Importantly, the levels of endogenous IAA in tomato seedlings significantly increased after AMF inoculation. Furthermore, the application of AMF significantly increased the expression levels of NADP-ME1 and NADP-ME2, as well as the activity of NADP-ME, and enhanced the root activity of tomato seedlings in comparison to that observed without inoculation of AMF. However, these effects were blocked in plants treated with 2,3,5-triiodobenzoic acid (TIBA), a polar transport inhibitor of IAA.Conclusion sThese results suggest that IAA mediates the AMF-promoted tomato growth and expression of NADP-MEs in continuous cropping substrates. The study provides convincing evidence for the reuse of continuous cropping substrates by adding AMF as an amendment.
研究领域:
植物科学
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农业
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真菌学
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园艺学
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产品关联
DOI
PubMed
高影响因子
Journal of Biobased Materials and Bioenergy
·
2017
·Vol. 11
·期 4
·282-290
·
IF 0.975
Zhang Panpan,Yuan Bo,Xu Mengke,Cao Xiaoying,Xue Lingwei,Jiang Jihong
This study aims to investigate the plant growth promoting bacteria (PGPB) in the medicinal plant Pinellia ternata (P. ternata), and evaluate the effect of PGPB on the growth and alkaloid content of P. ternata. Bacteria were isolated from P. ternate, and 16S
rRNA gene sequencing of partial isolates was carried out. 23 kinds of representative bacteria were classified into 7 genera: Acinetobacter, Bacillus, Enterobacter, Pseudomonas, Kosakonia, Rhizobium and Stenotrophomonas. The results of the plant growth promotion screening showed that
23 strains could produce different levels of Indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylicacid deaminase (ACC), biofilm and certain enzymes. Potential PGPB were selected to evaluate their abilities to promote the growth of wheat. The results indicated that strain PtB01 (Rhizobium
nepotum) and PtB24 (Bacillus methylotrophicus) significantly promoted the growth of the wheat seedlings. These two strains and their co-culture group were inoculated on P. ternata aseptic seedlings. It was found that these strains increased shoot length by 194%, root length
by 307%, leaf area by 232%, fresh weight by 231%, dry weight by 165%, and the number of fibrous roots by 200%. We also designed a novel organic silica hybrid monolithic column through silicon dioxide modification and tested the influence of two strains on the alkaloid in P. ternata
aseptic seedlings. The result showed that the content of alkaloid was increased by 140% after inoculation PGPB in P. ternata. PGPB from the medicinal plant P. ternata promoted the growth of the host plant and influenced the alkaloid content of P. ternata.
研究领域:
微生物学
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生药学
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分析化学
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植物科学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
·
IF 6
Dahe Qiao,Junyan Zhu,Xiaozeng Mi,Hui Xie,Mingtao Shu,Minyi Chen,Rui Li,Shengrui Liu,Chaoling Wei
Withering time of fresh leaves is a key factor affecting the stability of flavor quality of Taiping Houkui tea (TPHK). It's effect and mechanism on the formation of flavor in TPHK remain unelucidated. Here, metabolomics and transcriptomics were combined to comprehensively investigate changes in the main flavor compounds and the expression patterns of related genes under different withering times. The results showed that withering had the greatest influence on the content of most amino acids and aroma volatiles. The relative content of most volatiles was observed to be highest at 6–8 h of withering time, which was consistent with the results from the sensory evaluation of aroma quality. Plant hormone signal transduction pathways played a key role in the variation of the flavor compound content under different withering times, especially ABA and JA signals. WGCNA demonstrated that ABA, JA, IAA, SA and different flavor substances were significantly associated with specific modules. JA may be involved in the metabolism of theanine during withering, while ABA may be involved in the accumulation of aromatic amino acids and main aroma components of TPHK. This research may serve as a reference for the optimization of the processing technology for TPHK.
研究领域:
植物学
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遗传学
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食品科学
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分子生物学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 28
·6139-6147
·
IF 5.344
Fan Wang,Jian Yang,Yongsheng Li,Qixin Zhuang,Jinlou Gu
Enzyme-activated prodrug therapy (EAPT) is an effective cancer treatment strategy able to transport non-toxic prodrugs and subsequently convert them into drugs at specific times and locations. However, due to the limitation of easy biodegradability and the membrane-impermeable characteristic of exogenous enzymes, there is a need to exploit suitable carriers for the effective protection and simultaneous delivery of activating enzymes into cancer cells. Herein, hierarchically porous MOFs were employed for the loading of enzyme and prodrug in a single nanocarrier thanks to their different cavity sizes. The simple loading process allows entrapping of horseradish peroxidase (HRP) and a monocarboxyl-containing indole-3-acetic acid (IAA) prodrug with high loading capacities in different spaces, which keeps the catalytic activity of the enzyme perfectly intact and avoids the premature activation of the prodrug. The encapsulated HRP and IAA exhibit sustained and synchronized release behaviors. Compared to the native HRP enzyme, the current MOF nanocarriers not only facilitate enzyme delivery into cellular lysosomes and subsequent endosomal escape, but also effectively release enzyme and prodrug in the intracellular environment within 48 h. Eventually, HRP and IAA loaded MOF nanocarriers cause significant cell death with a low IC50 of 4.2 mg L−1, while the IAA prodrug alone is non-toxic even at high concentrations. Thus, hierarchically porous MOFs might offer a promising platform for EAPT with a highly consistent spatiotemporal distribution of enzymes and prodrugs in target tissues.
研究领域:
药学
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纳米技术
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癌症治疗
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材料科学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Mi Wei,Meng Zhang,Guobing Huang,Yuanyuan Yuan,Chunhua Fu,Longjiang Yu
The potentiality of PGPR and biocontrol bacteria in agriculture is steadily increased as it offers an attractive way to replace the use of chemical fertilizers, pesticides and other supplements. This study aimed to investigate the effects and potential mechanism of inoculation with two Bacillus velezensis strains on the growth and quality improvement of Anoectochilus roxburghii (Wall.) Lindl. (MRH) and Anoectochilus formosanus (Wall.) Lindl. (YYB). The control treatment was non-inoculated (CK) and the coculture treatments were inoculated with Bacillus velezensis D2WM (D2), Bacillus velezensis ZJ-11 (ZJ-11), Bacillus velezensis D2WM and Bacillus velezensis ZJ-11 (D2+ZJ-11). The fresh weight, plant length, amount of active compounds, as well as chlorophyll content were significantly increased under D2, ZJ-11, and D2+ZJ-11 treatments compared with MRH and YYB plants in CK. In particular, D2+ZJ-11 treatment resulted in the greatest growth promotion. In MRH and YYB plants, the fresh weight increased by 82.6 % and 106.6 %, the kinsenoside content increased by 9.33 % and 21.65 % per gram, and the flavonoid content increased by 44.70 % and 21.07 % per gram, respectively. D2WM and ZJ-11 were capable of secreting siderophore, phytase, indole-3-acetic acid (IAA), and zeatin to promote plant nutrient assimilation and growth. Moreover, when D2WM and ZJ-11 were both inoculated with Anoectochilus roxburghii , the rhizosphere soil enzyme activities of urease, phosphatase, and invertase were significantly higher than control, thus helped to provide more nutrients to Anoectochilus . Further, the analysis of microbial community diversity indicated the increase of abundance of beneficial microorganisms, such as nitrogen-fixing bacteria, Basidiomycota and Ascomycota both of which are known to fuel their plant partners with mineral nutrients from the soil. Additionally, the abundance of pathogens belonging to Xanthobacteraceae as well as Cladophialophora and Penicillium were reduced. Hence, coculture with beneficial microorganisms can improve the growth and quality of Anoectochilus plants via promoting nutrient assimilation and regulating rhizosphere microbial community. This technique is of great application value in promoting the efficient cultivation and production of high-quality plant materials.
研究领域:
农业
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微生物学
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植物科学
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产品关联
DOI
高影响因子
Stress Biology
·
2023
·Vol. 3
·期 1
·1-17
·
IF 0
Duan Kaili,Shen Qifang,Wang Yu,Xiang Ping,Shi Yutong,Yang Chenfei,Jiang Cong,Wang Guanghui,Xu Jin-Rong,Zhang Xue
Plant hormones are important for regulating growth, development, and plant-pathogen interactions. Some of them are inhibitory to growth of fungal pathogens but the underlying mechanism is not clear. In this study, we found that hyphal growth of Fusarium graminearum was significantly reduced by high concentrations of IAA and its metabolically stable analogue 2,4-dichlorophenoxyacetic acid (2,4-D). Besides inhibitory effects on growth rate, treatments with 2,4-D also caused significant reduction in conidiation, conidium germination, and germ tube growth. Treatments with 2,4-D had no obvious effect on sexual reproduction but significantly reduced TRI gene expression, toxisome formation, and DON production. More importantly, treatments with 2,4-D were inhibitory to infection structure formation and pathogenesis at concentrations higher than 100 µM. The presence of 1000 µM 2,4-D almost completely inhibited plant infection and invasive growth. In F. graminearum , 2,4-D induced ROS accumulation and FgHog1 activation but reduced the phosphorylation level of Gpmk1 MAP kinase. Metabolomics analysis showed that the accumulation of a number of metabolites such as glycerol and arabitol was increased by 2,4-D treatment in the wild type but not in the Fghog1 mutant. Transformants expressing the dominant active FgPBS2 S451D T455D allele were less sensitive to 2,4-D and had elevated levels of intracellular glycerol and arabitol induced by 2,4-D in PH-1. Taken together, our results showed that treatments with 2,4-D interfere with two important MAP kinase pathways and are inhibitory to hyphal growth, DON biosynthesis, and plant infection in F. graminearum .
研究领域:
植物病理学
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分子生物学
·
植物激素
✓ 已验证
DOI
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Fansheng Meng,Weiwei Huang,Dongfang Liu,Yingxin Zhao,Wenli Huang,Zhongfang Lei,Zhenya Zhang
In this study a continuous flow reactor (CFR) was employed to compare the feasibility of bacterial aerobic granular sludge (AGS-CFR) and algal-bacterial granular sludge (ABGS-CFR) for treating 1–4% saline wastewater. High salinity was found to enhance algae growth in ABGS-CFR, which exhibited slightly higher total nitrogen and phosphorus removal efficiencies at 1–3% salinity. ABGS-CFR maintained good granular stability at 1–4% salinity, while AGS-CFR gradually disintegrated at 4% salinity with 39.3% less accumulation of alginate-like exopolysaccharides in the extracellular polymeric substances. Indole-3-acetic acid (IAA) and superoxide dismutase (SOD) analysis suggested that bacteria and algae ( Nitzschia ) in ABGS-CFR formed a good symbiotic relationship under high salinity conditions, achieving rapid algae growth and 2 times lipid production. High salinity was conducive to enriching Halomonas and Nitzschia but unfavorable for Nitrosomonas and Flavobacterium . Results from this study could provide useful information on interactions between bacteria and algae in ABGS-CFR for its future practical application.
研究领域:
环境工程
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废水处理
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微生物学
·
生物技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）