SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Jiwen Hu,Zhangjun Hu,Sheng Liu,Qiong Zhang,Hong-Wen Gao,Kajsa Uvdal
We design and synthesize a new ratiometric fluorescent chemodosimeter ( S1 ) for the selective and sensitive detection of Hg 2+ . Upon addition of Hg 2+ , the emission of the S1 exhibits a large bathochromic shift from 393 to 515 nm (up to 122 nm) which is ascribed to an intramolecular charge transfer process in the resultant. The Hg 2+ -induced dethioacetalization for sensing mechanism has been demonstrated by using high-performance liquid chromatography analysis of the sensing process. The interference experiments further demonstrate that S1 exhibits very high selectivity towards Hg 2+ over other coexisting cations/anions. Subsequently, a good linearity of the concentrations of Hg 2+ (0–15 μM) vs the ratiometric signals ( I 515 / I 393 ) allows a fluorogenic method for the quantitative detection of Hg 2+ , with the limitation of detection determined to be 5.22 × 10 −7 M.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
·
IF 3.232
Simei Sun,Chaochao Qin,Hua Liu,Chao Jiang
Anthraquinone derivatives have been widely used as photocleavage agents and dyes. Here, photoinduced excited state intramolecular charge transfer (ICT) dynamics of 1-aminoanthraquinone in ethanol are studied by femtosecond transient absorption (fs-TA) spectroscopy and quantum chemical (QC) calculations. Four decay associated difference spectra and corresponding lifetime components were obtained by singular value decomposition and global fitting analysis from the fs-TA spectra. The QC calculations prove that the S 1 state has obvious ICT character. Planar ICT (PICT) and twisted ICT (TICT) reaction coordinates are observed. On the PICT coordinate, vibrational relaxation (VR) and intersystem crossing (ISC) processes are observed. The ISC efficiency is dependent on excitation wavelength, which elucidates that T′ 2 state participates in the ISC process on a higher-level than the S′ 1 state. On the TICT coordinate, the TICT process is dependent on excitation wavelength, which elucidates a direct experimental evidence of an energy barrier in excited state TICT potential energy.
研究领域:
化学
·
光化学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Cao Ting,Li Mengjin,Ma Hong,Fan Zhefeng
The liver is a vital metabolic and detoxification organ in the body, yet the diagnosis of drug-induced liver injury (DILI) remains challenging due to the limitations of traditional detection methods. Monoamine oxidases (MAOs), which are highly expressed on the mitochondrial membrane of hepatic tissues, are closely associated with DILI. In this study, a near-infrared ratiometric fluorescent probe Mito-1 was developed based on the intramolecular charge transfer (ICT) and excited-state intramolecular proton transfer (ESIPT) mechanisms. This probe could precisely target mitochondria, exhibiting high sensitivity (DL = 0.14 μg/mL), a wide linear detection range (10–150 μg/mL), excellent selectivity towards MAOs, and good biocompatibility. Cellular experiments confirmed its ability to distinguish MAO activity between normal and liver cancer cells. In an acetaminophen (APAP)-induced DILI zebrafish model, Mito-1 enabled in vivo dynamic visualization of MAO activity in hepatic tissues. Collectively, this work provides a novel visual tool for the early diagnosis of DILI and the mechanistic study of MAOs. Graphical
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 11
·5606
·
IF 4.9
Rong Wang,Kaiyue Song,Zhaoqin Wei,Yue Sun,Xiaoxia Sun,Yu Hu
The chiral H8-BINOL derivatives R-1and R-2were efficiently synthesized via a Suzuki coupling reaction, and they can be used as novel dialdehyde fluorescent probes for the enantioselective recognition of R/S-2-amino-1-phenylethanol. In addition, R-1is much more effective than R-2. Scanning electron microscope images and X-ray analyses show that R-1can form supramolecular vesicles through the self-assembly effect of the π-π force and strong hydrogen bonding. As determined via analysis, the fluorescence of the probe was significantly enhanced by mixing a small amount of S-2-amino-1-phenylethanol into R-1, with a redshift of 38 nm, whereas no significant fluorescence response was observed in R-2-amino-1-phenylethanol. The enantioselective identification of S-2-amino-1-phenylethanol by the probe R-1was further investigated through nuclear magnetic titration and fluorescence kinetic experiments and DFT calculations. The results showed that this mechanism was not only a simple reactive probe but also realized object recognition through an ICT mechanism. As the intramolecular hydrogen bond activated the carbonyl group on the probe R-1, the carbonyl carbon atom became positively charged. As a strong nucleophile, the amino group of S-2-amino-1-phenylethanol first transferred the amino electrons to a carbonyl carbocation, resulting in a significantly enhanced fluorescence of the probe R-1and a 38 nm redshift. Similarly, S-2-amino-1-phenylethanol alone caused severe damage to the self-assembled vesicle structure of the probe molecule itself due to its spatial structure, which made R-1highly enantioselective towards it.
研究领域:
超分子化学
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有机化学
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分析化学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Yinghong Zhou,Yanting Li,Lixue Yang,Xiaoxia Sun,Yu Hu,Yue Sun
An A-D-A π-conjugated fluorescent probe CBTOP, synthesized via Suzuki coupling and Knoevenagel condensation, serves as a specific tool for chlortetracycline (CTC) analysis. It emits fluorescence over 495–622 nm (blue-to-red). Mechanical grinding triggers its microstructure transformation from columnar to coral-like aggregates, identifiable by fluorescence intensity and color. Under varying water contents, CBTOP self-assembles into nanostructures (sheets, vesicles) via π-π stacking and hydrogen bonding. It specifically recognizes CTC with significant fluorescence quenching and emission blue shift, driven by the ICT mechanism (spectroscopically confirmed). With low cytotoxicity, it enables CTC fluorescent imaging in biological systems, offering a novel specific detection strategy with promising applications.
研究领域:
分析化学
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生物医学工程
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分子影像学
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药物分析
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纳米生物技术
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2024
·
IF 4.1
Xiu-Mei Song,Hai-Fei Ye,Jiang-Li Song,Yan-Jie Xi,Yancheng Wu
A novel sensor (Sensor 2 ) containing a fluorescent core of aromatic naphthalene and a binding site of aminothiourea group has been synthesized and systematically investigated with regard to anions recognition. The results indicate that Sensor 2 shows fast response, high selectivity and sensitivity and reversibility to detect fluoride (F − ) and cyanide (CN − ). Adding F − /CN − into DMSO solution of Sensor 2 induces a distinct color change from colorless to yellow under ambient light and from blue to greenish-blue under 365 nm UV light. Besides, Sensor 2 can distinguish F − from CN − by fluorescent spectra based on the different sensitivities of Sensor 2 to F − and CN − , as well as “turn on” at the different emission wavelengths. The sensing mechanism has been proposed and verified by ESI-MS, 1 H NMR titration, DFT and TD-DFT calculations, which refers to the interaction of Sensor 2 with F − and CN − via forming hydrogen bond and subsequent deprotonation. The reversibility of Sensor 2 was also studied, confirming that it is a reusable sensor. In addition, the preliminary application of Sensor 2 for the detection of fluoride in toothpaste was successful.
研究领域:
分析化学
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材料科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Xitao Xia,Fang Zeng,Peisheng Zhang,Jun Lyu,Yong Huang,Shuizhu Wu
Hydrazine is a widely used industrial material, and yet it is also a highly toxic and possibly carcinogenic substance which is likely to induce physiological dysfunction. Here, we report a ratiometric fluorescent probe (NA-N 2 H 4 ) for hydrazine detection based on the intramolecular charge transfer (ICT) mechanism. As a naphthalimide derivative with an acetate group at the 4th position of its naphthalic ring, this probe can realize both colorimetric and ratiometric detection for hydrazine. The probe NA-N 2 H 4 exhibits a very low mean detection limit of 9.40 ± 0.12 nM, which is well below the safety level (10 ppb, or 312 nM) as regulated by EPA. The probe's selectivity and anti-interference performance is found desirable, and the probe works well in quantitative detection toward hydrazine in real samples (drinking water and river water), as well as in the qualitative detection of gaseous hydrazine. Moreover, it can be employed to image hydrazine in living cells and in vivo (zebrafish).
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产品关联
DOI
高影响因子
Cancers
·
2022
·Vol. 14
·期 23
·5951
·
IF 6.575
Qiyi Yi,Youguang Pu,Fengmei Chao,Po Bian,Lei Lv
Simple SummaryACAP1 plays a key role in endocytic recycling, which is essential for the normal function of lymphocytes. This study aimed to assess the expression and function of ACAP1 in lymphocytes. In this study, we revealed that ACAP1 is expressed primarily in lymphocytes and is necessary for the normal function of lymphocytes. Its expression is regulated by promoter DNA methylation and the transcription factor SPI1. ACAP1 levels positively correlate with the infiltrating levels of tumor-infiltrating lymphocytes (TILs) across a broad range of solid cancer types. Moreover, ACAP1 deficiency is associated with poor prognosis and immunotherapeutic response in multiple cancer types treated with immunotherapy. Thus, this study demonstrates that ACAP1 is a novel lymphocyte marker. We propose a widely applicable indicator to predict response to immunotherapy that may guide cancer patient stratification for appropriate therapy.AbstractBackground: ACAP1 plays a key role in endocytic recycling, which is essential for the normal function of lymphocytes. However, the expression and function of ACAP1 in lymphocytes have rarely been studied. Methods: Large-scale genomic data, including multiple bulk RNA-sequencing datasets, single-cell sequencing datasets, and immunotherapy cohorts, were exploited to comprehensively characterize ACAP1 expression, regulation, and function. Gene set enrichment analysis (GSEA) was used to uncover the pathways associated with ACAP1 expression. Eight algorithms, including TIMER, CIBERSORT, CIBERSORT-ABS, QUANTISEQ, xCELL, MCPCOUNTER, EPIC, and TIDE, were applied to estimate the infiltrating level of immune cells. Western blotting, qPCR, and ChIP-PCR were used to validate the findings from bioinformatic analyses. A T-cell co-culture killing assay was used to investigate the function of ACAP1 in lymphocytes. Results: ACAP1 was highly expressed in immune-related tissues and cells and minimally in other tissues. Moreover, single-cell sequencing analysis in tumor samples revealed that ACAP1 is expressed primarily in tumor-infiltrating lymphocytes (TILs), including T, B, and NK cells. ACAP1 expression is negatively regulated by promoter DNA methylation, with its promoter hypo-methylated in immune cells but hyper-methylated in other cells. Furthermore, SPI1 binds to the ACAP1 promoter and positively regulates its expression in immune cells. ACAP1 levels positively correlate with the infiltrating levels of TILs, especially CD8+ T cells, across a broad range of solid cancer types. ACAP1 deficiency is associated with poor prognosis and immunotherapeutic response in multiple cancer types treated with checkpoint blockade therapy (ICT). Functionally, the depletion of ACAP1 by RNA interference significantly impairs the T cell-mediated killing of tumor cells. Conclusions: Our study demonstrates that ACAP1 is essential for the normal function of TILs, and its deficiency indicates an immunologically “cold” status of tumors that are resistant to ICT.Keywords:ACAP1;tumor-infiltrating lymphocytes (TILs);immune checkpoint blockade therapy (ICT);cancer immunotherapy;CD8+ T cells
研究领域:
肿瘤学
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免疫学
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癌症生物学
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分子生物学
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基因组学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Advances
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2022
·
IF 0
Qing Wang,Xiaoyan Bian,Hang Yao,Xiaohui Yuan,Ying Han,Chaoguo Yan
Three protein microenvironment-sensitive pillar[5]arene-based fluorescent probes (3/4/5C-B) were designed and synthesized based on intramolecular charge transfer (ICT) mechanism. Unlike the majority of micromolecular ICT probes, the aforementioned probes displayed differentiated sensitivity to multiple proteins. The 7-(diethylamino)coumarin-3-formic acid (DCCA) group in the probes was essential for their sensitivity. The presence of a pillar[5]arene group was also crucial as they benefit 3/4/5C-B form complexes with the proteins, although it changed the electron density distribution of the DCCA group. 3/4/5C-B exhibited favorable carrier ability for regorafenib (REG). 4C-B had the best spatial structure for complexation. The 3/4/5C-B-REG complexes would assemble into high drug-loading fluorescent nanoparticles in a physiological environment (pH = 7.4). Such nanoparticles exhibited pH-triggered enrichment ability, which rapidly enriched REG in the acidic environment (pH = 6.0). Moreover, the complexation between 3/4/5C-B and REG maintained the live-cell membrane imaging property of the probes and the excellent targeted anticancer activity of the drug.
研究领域:
生物化学
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分子生物学
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药学
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细胞生物学
·
纳米技术
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DOI
PubMed
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Tao Ma,Fangjun Huo,Ying Wen,Timothy E. Glass,Caixia Yin
Endogenous H 2 S, considered to be involved in many physiological processes, has attracted more attention in fluorescence detection and bioimaging. Therefore, it is necessary to design probes with good biocompatibility and high bioavailability. In this study, a novel fluorescent probe , QN-1 , based on azide group and quinoline derivatives was developed for detecting H 2 S. QN-1 can detect H 2 S specifically in aqueous phase, which indicated QN-1 has excellent water solubility. Besides, QN-1 shows excellent properties of higher selectivity and 11-fold fluorescence enhancement at 533 nm. Therefore, QN-1 with excellent properties can be used for cell imaging.
研究领域:
生物化学
·
医学影像学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Ting Cao,Mengjin Li,Hong Ma,Zhefeng Fan
Monoamine oxidase (MAOs) is a key enzyme that maintains amine homeostasis and participates in liver pathological processes. Real time in situ monitoring of its activity is crucial for elucidating the mechanism of drug-induced liver injury (DILI). This study successfully constructed a ratio type fluorescent probe Cy-1 with mitochondrial targeting ability and near-infrared emission characteristics. This probe is based on MAOs catalyzed oxidative deamination reaction, and achieves fluorescence response through a clever “ICT (Intramolecular Charge Transfer) + ESIPT (Excited-State Intramolecular Proton Transfer)” dual mechanism, producing significant ratiometric signal changes in the near-infrared region. Experiments have shown that Cy-1 has a wide linear range (10–115 μg/mL), high sensitivity (0.86 μg/mL), and high selectivity in vitro, and can specifically localize to mitochondria. The probe was successfully used for in situ differential monitoring of endogenous MAOs activity in living cells, and visualized semi quantitative monitoring of MAOs activity changes in vivo was achieved in an acetaminophen (APAP) induced drug-induced liver injury (DILI) model. Cy-1 is expected to become a powerful chemical tool for understanding the mechanisms of MAOs in physiological and pathological processes, especially in the occurrence and development of diseases such as liver injury, as well as evaluating the efficacy of related drugs.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Haixia Wu,Yuze Chen,Xunli Ling,Weiwei Yuan,Bowen Li,Zhan Zhou
In order to explore the application of D-π-A molecules in bio-imaging, two novel D-π-A and ICT type molecules ( SE and PE ) are designed and synthesized by the adjustment of the π conjugation bridge. Because of strong intramolecular charge transfer (ICT) effect between the carbazole and pyrimidine unit, SE and PE exhibit weak fluorescence emission in the aqueous solution system. Interestingly, SE shows the enhanced fluorescence detection performance for hypochlorite due to “D-π-A" structure and suitable ICT effect after the oxidative cleavage of alkene unit by hypochlorite. Importantly, the cell membrane permeable SE can be used for the exogenous and endogenous ClO − in living cells and zebrafishes without apparent toxicity before and after treating with ClO − . Our study indicates that regulating “D-π-A" structure and suitable ICT effect of the organic fluorescence probes is a powerful method to enhance their detection performance for the target analyte.
研究领域:
生物成像
·
有机化学
·
荧光传感
·
化学生物学
·
分析化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）