JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Qiaozhi Zhang,Zhijie Huang,Huatao Li,Congnan Cen,Ruixing Zheng,Cao Lili,Shufen Zhang,Yanbo Wang,Linglin Fu
As a complex reaction, biological consequences of the Maillard reaction (MR) on dietary proteins need to be deciphered. Despite previous studies on the structural and antigenic properties of ovalbumin (OVA) by MR, associated changes induced by specific MR intermediates and their downstream products are largely unknown. This study focused on the impacts of glycation by α-dicarbonyl compounds (α-DCs), intermediates of MR and precursors of advanced glycation end-products (AGEs), on the structural and IgE-binding properties of ovalbumin (OVA) under simulated heating. Methylglyoxal (MGO), glyoxal (GO), and butanedione (BU) were selected as typical α-DCs to generate glycated OVA with different AGE-modifications (AGE-Ms). The results showed that reactions between OVA and α-DCs generated OVA-AGE with various degrees of modification and conformational unfolding, and the reactivity of α-DCs followed the order GO > MGO > BU. Depending on the precursor type, the levels of 10 specific AGEs were verified, and the amounts of total AGEs increased with heating temperature and α-DC dosage. Compared to native OVA, glycated OVA showed reduced IgE-binding levels but with sRAGE-binding ligands, the extent of which was associated with the contents of total AGEs and Nε-carboxymethyllysine, and changes in certain protein conformational structures. High-resolution mass spectrometry further identified different AGE-Ms on the Lys and Arg residues of OVA, confirming variations in the glycation sites and their associations with the immunoreactive epitopes of OVA under different conditions.
研究领域:
食品科学
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免疫学
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蛋白质化学
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生物化学
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PubMed
高影响因子
Frontiers in Immunology
·
2022
·
IF 8.786
Yang Yong-Shi,Cao Meng-Da,Wang An,Liu Qing-Mei,Zhu Dan-Xuan,Zou Ying,Ma Ling-Ling,Luo Min,Shao Yang,Xu Dian-Dou,Wei Ji-Fu,Sun Jin-Lyu
Background: Allergic respiratory diseases have increased dramatically due to air pollution over the past few decades. However, studies are limited on the effects of inorganic components and particulate matter with different particle sizes in smog on allergic diseases, and the possible molecular mechanism of inducing allergies has not been thoroughly studied.Methods: Four common mineral elements with different particle sizes in smog particles were selected, including Al2O3, TiO2, Fe2O3, and SiO2. We studied the relationship and molecular mechanism of smog particle composition, particle size, and allergic reactions using mast cells, immunoglobulin E (IgE)-mediated passive cutaneous anaphylaxis (PCA) model, and an ovalbumin (OVA)-induced asthmatic mouse model in vitro and in vivo, combined with transmission electron microscopy, scanning transmission X-ray microscopy analysis, and transcriptome sequencing.Results: Only 20 nm SiO2 particles significantly increased β-hexosaminidase release, based on dinitrophenol (DNP)-human serum albumin (HSA) stimulation, from IgE-sensitized mast cells, while other particles did not. Meanwhile, the PCA model showed that Evan’s blue extravasation in mice was increased after treatment with nano-SiO2 particles. Nano-SiO2 particles exposure in the asthmatic mouse model caused an enhancement of allergic airway inflammation as manifested by OVA-specific serum IgE, airway hyperresponsiveness, lung inflammation injury, mucous cell metaplasia, cytokine expression, mast cell activation, and histamine secretion, which were significantly increased. Nano-SiO2 particles exposure did not affect the expression of FcϵRI or the ability of mast cells to bind IgE but synergistically activated mast cells by enhancing the mitogen-activated protein kinase (MAPK) signaling pathway, especially the phosphorylation levels of the extracellular signal-regulated kinase (ERK)1/2. The ERK inhibitors showed a significant inhibitory effect in reducing β-hexosaminidase release.Conclusion: Our results indicated that nano-SiO2 particles stimulation might synergistically activate IgE-sensitized mast cells by enhancing the MAPK signaling pathway and that nano-SiO2 particles exposure could exacerbate allergic inflammation. Our experimental results provide useful information for preventing and treating allergic diseases.
研究领域:
免疫学
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环境健康
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分子生物学
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过敏与哮喘
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PubMed
高影响因子
Chinese Medicine
·
2025
·Vol. 20
·期 1
·1-22
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IF 5.7
Li Xueyan,Ding Lu,Li Zirui,Cao Zhenghua,Li Min,Yin Kai,Song Siyu,Cao Liyuan,Xia Qinjing,Wang Zihan,Zhao Daqing,Tong Xiaolin,Li Xiangyan,Wang Zeyu
Background Asthma is a chronic inflammatory airway disease that remains inadequately controlled by existing conventional treatments. A traditional Chinese medicine (TCM) formula of Yangke powder (yǎng ké sǎn-YKS) has demonstrated potential in alleviating asthma symptoms and reducing its acute exacerbation. Despite clinical evidence supporting its benefit, there is still insufficient understanding of the active compounds in YKS and their underlying mechanisms, which limits its broader clinical application. Objective This study aims to identify the key active ingredients in YKS and explore their mechanisms, particularly through the PI3K/AKT/NF-κB pathways, to provide a scientific basis for its application in asthma treatment. Methods We employed UPLC-Q-Exactive Orbitrap-MS to analyze YKS constituents, identified key ingredients, and explored asthma treatment mechanisms through bioinformatics, network pharmacology, Mendelian randomization, and molecular docking. The asthma model was evaluated using ovalbumin (OVA) and pulmonary function tests, while pathological examination was conducted using hematoxylin and eosin (HE), periodic acid-Schiff (PAS), and Masson trichrome stains. Concentrations of IgE, IL-4, and IL-5 were measured by ELISA, and protein and mRNA expressions were confirmed via qPCR, immunohistochemistry, and Western blot analysis. Results A total of 174 compounds were identified in YKS by UPLC-MS, with 49 detected in the bloodstream, indicating their role as active ingredients. Bioinformatics analysis revealed 353 asthma-related targets and 972 potential targets for YKS. Key targets such as AKT1, TNF, and IL1B were validated by molecular docking. Our studies indicated that YKS modulates asthma primarily through the PI3K/Akt and NF-κB pathways, improving airway resistance, reducing inflammation, mucus production, and airway remodeling, and decreasing Th2 cytokines and IgE levels. Conclusion This investigation identifies Kaempferol, Norephedrine, Cynaroside, Genistein, and Rutin as critical active ingredients in YKS, impacting key biomarkers such as AKT1, TNF, and IL1B. These substances effectively modulate the PI3K/AKT/NF-κB pathway, enhancing the management of allergic asthma.
研究领域:
药理学
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免疫学
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呼吸病学
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2026
·
IF 8.3
Yu Lu,Guangyao Chen,Liwei Xue,Wenxia Zhang,Jing Zhang,Ge Dai,Hao Shen,Zhengrong Chen,Liguo Chen
Sample-to-answer centrifugal microfluidics enables whole blood sIgE testing. • Euler force-driven siphon valves enable actuator free programmable release. • Backflow-suppressed blood separation stabilizes on-chip immunoassays. • Clinical validation demonstrates sample-to-answer allergen-sIgE detection. Allergen-specific immunoglobulin E (sIgE) detection is essential for the diagnosis and management of type I hypersensitivity diseases. However, conventional enzyme-linked immunosorbent assay (ELISA)-based sIgE assays remain limited by laborious blood pretreatment and bulky analytical instruments, restricting their application in point-of-care testing (POCT). Here, we developed an integrated centrifugal microfluidic system (ICMS) for fully automated sample-to-answer detection of allergen-sIgE directly from whole blood. The platform integrates an optimized on-disc blood separation module with Euler force-driven siphon valving strategy to automate whole blood pretreatment, sequential reagent delivery, mixing, incubation, washing, and colorimetric detection. The optimized separation structure enabled efficient serum extraction while suppressing blood cell backflow. Meanwhile, the Euler force-driven siphon valves with tunable triggering thresholds enabled precise multistep fluid manipulation without external actuators, supporting a reliable automated ELISA workflow. The ICMS comprised eight parallel, independently configurable assay units, enabled completion of the entire workflow within 70 min, and was validated for detecting sIgE against cow’s milk, Dermatophagoides pteronyssinus , and Dermatophagoides farinae . Clinical validation showed good agreement with conventional clinical methods, with a correlation coefficient of R 2 = 0.9813 and an overall grading accuracy of 85.19%. Larger-scale clinical studies are needed to further evaluate the diagnostic performance and clinical applicability of the ICMS. These results demonstrate that the proposed ICMS provides a highly integrated, automated, and reliable sample-to-answer platform for direct whole blood allergen-sIgE detection, showing considerable potential for POCT applications in primary healthcare settings. Download: Download high-res image (205KB) Download: Download full-size image
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高影响因子
Food & Function
·
2023
·
IF 6.1
Xian Xia,Jiangdong Li,Rui Liang,Yi Li,Xiaojuan Ma,Ying Yang,Daniel Lozano-Ojalvo
Ovomucoid is the immune-dominant allergen in the egg white of hens. Due to its structure based on nine disulfide bonds as well as its resistance to heat and enzymatic hydrolysis, the allergenicity of this food protein is difficult to decrease by technological processes. We sought to reduce its allergenicity through the Maillard reaction. The unfolding of ovomucoid with L-cysteine-mediated reduction was used to increase accessibility to conformational and linear epitopes by modifying the secondary and tertiary structures of the allergen. Glycation with different saccharides revealed the beneficial effect of maltose glycation on the IgG-binding capacity reduction. By determining the better glycation conditions of unfolded ovomucoid, we produced ovomucoid with reduced IgE binding capacity due to the glycation sites (K17, K112, K129, and K164) on epitopes. Moreover, after simulated infant and adult gastrointestinal digestion, the unfolded plus glycated ovomucoid showed higher ABTS˙+ scavenging activity, O2˙− scavenging activity, ˙OH scavenging activity, Fe2+ chelating activity, and a FRAP value; in particular, for ˙OH scavenging activity, there was a sharp increase of more than 100%.
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
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IF 7.3
Jun Zhang,Huiting Lian,Guangming Liu,Qingmei Liu,Bin Liu,Xiaofeng Wei
This study reports an integrated dual-signal microfluidic immunosensor for point-of-care detection of ovalbumin-specific IgE (OVA-sIgE) in interstitial fluid. A pH-responsive composite material, ZIF-8 encapsulating phenolphthalein and surface-conjugated with antimouse OVA-sIgE (ZIF-8@PP∼Abs), was synthesized and characterized. Structural and elemental analyses confirmed the successful synthesis and antibody functionalization, while the composite retained strong alkaline-triggered colorimetric response due to ZIF-8 decomposition and subsequent phenolphthalein release. Carbon dots (CDs) show concentration-dependent fluorescence, which is effectively quenched by ZIF-8@PP∼Abs. A paper-based chip coimmobilized with CDs and OVA antigen enabled simultaneous colorimetric and fluorescent detection. The colorimetric pathway operates as a “turn-on” system through alkaline-induced color change, while the fluorescent pathway functions as a “turn-off” system via CDs quenching by ZIF-8@PP∼Abs. Quantitative analysis using the green channel─identified as the most sensitive through RGB decomposition─showed linear responses across 0.06–8.00 ng/mL OVA-sIgE, with detection limits of 0.026 ng/mL (colorimetric) and 0.032 ng/mL (fluorescence). The dual-signal design, featuring built-in cross-verification, demonstrates high reliability through favorable spike-and-recovery results. ROC analysis shows AUC values of 0.9999 for both signals, with a significant reduction in false positives. To achieve operational integration, a custom-designed negative-pressure-driven microfluidic chip was developed, incorporating all necessary steps into a negative-pressure valve-controlled microfluidic chip. The chip demonstrated robust fluid handling, repeatable operation, and user-friendly functionality. The integrated sensor exhibited excellent selectivity, strong anti-interference capability against common interstitial fluid constituents. This work presents a practical, accurate, and reliable platform for decentralized allergy testing, merging advanced nanomaterials, dual-mode transduction, and microfluidic engineering.
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高影响因子
Food Science and Human Wellness
·
2023
·
IF 7
Linglin Fu,Rongrong Wang,Jinru Zhou,Chong Wang,Yanbo Wang
N -glycans in many proteins are of great concern because of their strong association with food allergies. Triticum aestivum (bread wheat), a major food crop, is known as one of the “Big Eight” allergenic groups. However, little research has been done about N -glycans in wheat glycoproteins. In this study, a soluble wheat glycoprotein was purified from wheat and further identified as globulin-1 S allele (GSA). The wheat GSA displayed significant IgE-binding activity. Moreover, one N -glycosylation site and 6 kinds of N -glycans were identified by mass spectrometry, including 3 high mannose types and 3 complex types. Furthermore, the IgE-binding activity of wheat GSA is proved to be reduced by the removal of N -glycan, thermal treatment (temperatures > 80 °C), and strong acidic treatment (pH 3.0). These findings would provide a better understanding of the effects of N -glycosylation, thermal treatment, and acidic treatment on the molecular characteristics of GSA, and further provide new insights into the development of hypoallergenic wheat products.
研究领域:
食品科学
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植物生物学
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免疫学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2012
·Vol. 404
·期 3
·887-893
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IF 3.778
Hu Xia,Wu Gong-ping,Zhang Meng-hui,Pan Shan-qing,Wang Rong-rong,Ouyang Jie-hu,Liu Jun-ge,Chen Zi-yuan,Tian Hong,Liu Dong-bo
Clinical definition and appropriate management of anaphylaxis is a clinical challenge because there is large variability in presenting clinical signs and symptoms. Monitoring of the metabolic status of anaphylaxis may be helpful in understanding its pathophysiological processes and diagnosis. The purpose of this study was to conduct GC–MS serum metabolic profiling of anaphylaxis animal models and search for potential biomarkers of anaphylaxis. Thirty-six guinea pigs were randomly divided into an ovalbumin group ( n = 12), a cattle albumin group ( n = 12), and a control group ( n = 12). The IgE level in the serum of the guinea pigs was evaluated by use of ELISA kits and the major metabolic changes in serum were detected by gas chromatography–mass spectrometry. Typical clinical symptoms appeared after the animals had been challenged with ovalbumin or cattle albumin. The IgE levels in serum of both model groups were significantly higher than those of the control group. Clustering trend of the three groups based on variables was observed and nine out of 858 metabolomic features were found to be significantly different between control group and model groups. Among the nine features, six features were tentatively identified as metabolites related to energy metabolism and signal transduction in anaphylaxis. In conclusion, GC–MS-based metabolic profiling analysis might be an effective auxiliary tool for investigation of anaphylaxis.
Chromatographic profile of the derivatized serum
研究领域:
临床医学
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代谢组学
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DOI
高影响因子
Food Bioscience
·
2024
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IF 4.8
Caiying Yang,Ying Zhang,Xiaoya Zhou,Zhihua Wu,Jinyan Gao,Hongbing Chen,Ping Tong
Allergen modification is a novel strategy for preventing and treating food allergy. Resveratrol (RES) can be used as a modifying ligand for allergens, but the interaction mechanism between RES and allergens is still not detailed, and the ramifications of this interaction on the structure, epitope and allergenicity of allergens are also lacking a clear explanation. In addition, the application of heat can promote protein structure to unfold and expose more binding sites to facilitate interaction. Therefore, in this study, ovalbumin (OVA) was selected as the allergen model and was heated to expose more binding sites. The effects of the interaction between RES and heat-stressed ovalbumin (HOVA) on allergen structure, epitope and allergenicity were investigated by combining routine experimental analysis and computer simulation. Results showed heat stress at 323 K for 15 min could better unfold the molecular structure of OVA. On this basis, the interaction between RES and OVA was strengthened, which showed a static quenching of the ground state complex with binding affinity of 1.25 × 10 6 L/mol. The binding of RES induced the variation of protein microenvironment, and the overall structure tended to disordered direction. Computer simulation had shown RES could directly act on the IgE epitope region (AA251-260) of the OVA. The allergenicity evaluation experiment further indicated the combination of RES alleviated the IgE binding capacity of HOVA. These findings are helpful to further study the mechanism of RES ligand modification to change food allergy.
研究领域:
食品科学
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过敏反应
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生物化学
·
分子生物学
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高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6
Ruijie Liu,Zixin Liang,Shujun Xia,Yuxiang Luo,Huilin Li
Background Proteoform-level analysis of large, highly modified proteins (≥50 kDa) such as immunoglobulin E (IgE) remains challenging due to their size and molecular heterogeneity. While traditional bottom-up proteomics fails to retain proteoform-level information, top-down approaches often lack the resolution required for effective proteoform discrimination. Middle-down proteomics (MDP) overcomes these limitations by generating highly redundant peptides in the 3–15 kDa range, which retain critical combinatorial modification patterns, thereby enabling more comprehensive characterization of complex proteoforms. Results To advance heterogeneity analysis of large proteins, we developed an online, tunable pepsin digestion platform that facilitates rapid and in-depth middle-down analysis of large proteins. When applied to IgE—a key allergy-related antibody with a large molecular weight (170–190 kDa) and bearing seven potential N-glycosylation sites—this platform produces high-redundancy peptides, enabling de novo sequencing of the variable region, which is critical for antigen recognition. Furthermore, analysis of middle-sized peptides allowed us to detect combined glycosylation patterns on the constant region 3 of the epsilon heavy chain (Cε3), revealing 39 Cε3 domain proteoforms. These proteoforms are important for Fcε receptor I interaction, therapeutic antibody binding, and downstream signaling. The platform also provided comprehensive glycosylation insights, including composition, occupancy, and microheterogeneity, thereby supporting detailed annotation of intact IgE proteoforms with varying glycan numbers. Significance By integrating sequence and glycosylation information, our approach offers a holistic perspective of IgE heterogeneity, which is essential for elucidating their roles in allergic diseases and for guiding diagnostic and therapeutic development. This study demonstrates that MDP analysis is highly valuable for large, diverse proteins like IgE. Our workflows enhance methodological versatility by minimizing dependence on specific proteases for particular proteins, thereby broadening the potential applications of MDP.
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PubMed
高影响因子
EXPERIMENTAL ANIMALS
·
2021
·
IF 2.319
Jianhua Wu,Lizhen Wu,Li Zhang,Huanhuan Xu,Min Wang,Lin Wang,Jie Chen,Kaiyue Sun
Inflammatory allergic reaction is the main cause of allergic rhinitis (AR). Previous studies indicated that miR-224-5p was downregulated in the nasal mucosa of patients with AR, while the function of miR-224-5p in AR remains unclear. To explore this issue, AR mouse model was established using ovalbumin (OVA). For treatment group, lentivirus (LV)-miR-224-5p or its control was intranasally administrated to AR mice. miR-224-5p expression was detected by reverse transcription-quantitative PCR, followed by assessing the immunoglobulin E (IgE) level. Pathological alterations in nasal mucosa were detected using Hematoxylin-Eosin staining and Sirius red staining, followed by assessing the levels of inflammatory cells and factors. The NLRP3 inflammasome and TLR4/MyD88/NF-κB pathway were measured by Western blot, and then the relationship between miR-224-5p and toll-like receptor 4 (TLR4) was verified. The results showed that miR-224-5p was significantly decreased in nasal mucosa of AR mice. AR mice exhibited increased sneezing and nasal rubbing events, IgE level in serum, and pathological alterations in nasal mucosa, while overexpression of miR-224-5p markedly attenuated these changes. The levels of inflammatory cells in nasal lavage fluid and pro-inflammatory factors in serum and nasal mucosa were significantly increased in AR mice, which were reduced by miR-224-5p overexpression. Of note, LV-miR-224-5p treatment remarkably suppressed the activations of NLRP3 inflammasome and the TLR4/MyD88/NF-κB pathway in AR mice. Furthermore, miR-224-5p could bind to 3’-untranslated region (3’-UTR) of TLR4 and negatively regulate TLR4 level. Overall, we conclude that miR-224-5p may relieve AR by negatively regulating TLR4/MyD88/NF-κB pathway, indicating that miR-224-5p may be a promising target for AR treatment.
研究领域:
免疫学
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分子生物学
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过敏
·
药理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Enning Zhou,Weiwei Wang,Xiaofeng Xue,Pianpian Wang,Fan Wu,Liming Wu,Qiangqiang Li
Bee pollen is a byproduct of pollination, which is a necessary process to produce foods. However, bee pollen can induce significant food-borne allergies. We previously identified a bee pollen-derived pan-allergen in the profilin family, Bra c p. Herein, we aimed to reduce Bra c p allergenicity via protein oxidation with hydrogen peroxide and explore the changes induced. Ion-mobility mass spectrometry revealed aggregation of the oxidized product; we also found irreversible sulfonation of the free sulfhydryl group of the Bra c p Cys98 residue to a more stable cysteine derivative. A significant proportion of the α-helices in Bra c p were transformed into β-sheets after oxidation, masking the antigenic epitopes. An immunoassay demonstrated that the IgE-binding affinity of Bra c p was decreased in vitro after oxidation. To our knowledge, this is the first report describing the application of protein oxidation to reduce the allergenicity of profilin family member in foods.
研究领域:
过敏反应
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免疫学
·
蛋白质化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
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