MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-8
·
IF 5.479
Wang Kexin,Ding Yadan,Hong Xia,Liu Yichun
A reliable, rapid and ultrasensitive immunoassay is described for determination of immunoglobulin G (IgG). It is making use of biofunctional magnetite (Fe 3 O 4 ) superparticles coated with SiO 2 and serving as an infrared (IR) probe. The unique IR fingerprint signals originating from the transverse and longitudinal phonon modes, respectively, of the asymmetric stretching of the Si–O–Si bridges display a satisfactory resistance to optical interference from the environment. The adoption of Fe 3 O 4 superparticles instead of Fe 3 O 4 nanoparticles as the magnetic core warrants a controllable structure and a strong magnetic response. This facilitates the efficient purification of the probes and the alleviation of the interfacial resistance between the liquid-solid interfaces by using a magnet. The gold-coated substrate was used to immobilize goat-anti-human IgG. The analyte (human IgG) was incubated with the IR probes, and then captured by the substrate immobilized antibody with the assistance of an external magnetic field. The integral area of the IR absorption band between 1250 cm −1 – 900 cm −1 was chosen for quantitative assay. The limit of detection is 95 fM, which is two orders of magnitude better than that without the magnetic field. The assay time was shortened from 2 h to 1 min. High selectivity, specificity, and long-term stability of the immunoassay were achieved. The performance of the assay when analyzing blood samples confirmed the practicability of the method.
Schematic presentation of the infrared (IR) immunoassay based on Fe 3 O 4 superparticle@SiO 2 nanocomposites. The assistance of an external magnetic field reduces the incubation time and improves the detection sensitivity.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Sizhu Tian,Xuwen Li,Jia Jiang,Li Tang,Hanqi Zhang,Yong Yu,Ziwei Zhang
Water soluble copper sulfide (CuS) nanoparticles were synthesized using l -cysteine as the ligand. Multiple biotins were conjugated to the antibody of rabbit IgG, and the streptavidin was attached to the CuS nanoparticles. The Cu 2+ ions enclosed in the nanoparticles were used as the electron spin resonance (ESR) probes and detected with ESR spectrometer. The immunoassay reaction was resulted in the formation of the coating antibody attached to the microplate well, the detecting antibody labeled with the biotins, and the streptavidin attached to the CuS nanoparticles. After the immunoassay reaction was performed, large amount of Cu 2+ /Cu + ions inside the nanoparticles were released with the help of diethyldithiocarbamate (DDC) and the Cu 2+ -DDC complex formed. The Cu 2+ -DDC complex was extracted into n -butanol, which was used as the analytical sample. Both ESR and UV–vis signals were collected for the analytical sample. The double logarithm standard curve was well simulated with a linear regression equation. The limits of detection of the rabbit IgG were 1.76 pg/mL and 2.36 pg/mL by ESR and UV–vis method, respectively. The detection range using ESR as the detector was from 8.8 pg/mL to 500 ng/mL, covering almost 5 magnitude orders of the rabbit IgG concentrations. The rabbit serum was analyzed and the rabbit IgG concentration was found to be 7.76 mg/mL. The reproducibility of the present method was good enough with the intra-assay error within 3.4 % and the inter-assay error within 11.2 %. The spiked serum samples were analyzed and the experimental results indicated that the recoveries were from 108.2 to 113.7%.
研究领域:
生物化学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Yongwei Zhang,Ziyue Li,Aolin Zhu,Qian Zhang,Hao Zhou,Xuelei Zhou,Tingwei Liu,Chunyan Liu,Hefei Zha,Xin Zhang,Hui Zhao,Jiutong Li,Guodong Lü,Xinxia Li
Brucellosis is a bacterial infectious disease caused mainly by Brucella. Transmission is mainly by contact with infected domestic or wild animals or their excreta. Clinical diagnosis of brucellosis is usually based on qualitative total antibody tests, which make it difficult to differentiate between acute and pre-existing infections. In this study, for the simultaneous detection of anti-brucellosis IgG and IgM, we innovatively developed a surface-enhanced Raman scattering (SERS)-lateral flow immunoassay (LFIA) detection system, and evaluated its performance and diagnostic effect by using clinical serum samples. The key design of this biosensor involves the preparation of two immunoprobes using two Raman spheres (R-Sphere) with distinct signals conjugated to mouse anti-human IgG and IgM, respectively. In this design, brucellosis-specific antigens are embedded in the T-line, allowing simultaneous capture of anti-brucellosis IgG and IgM in a specific binding reaction. A portable Raman instrument is used to detect the characteristic signal intensities generated by IgM and IgG on the T-line, which can then be used to analyze the target IgM and IgG antibodies. Based on the analysis of 40 clinical samples, the method has a sensitivity and specificity of 100%, and the detection time is only 15 min. The advantages of this technology are fast speed, convenient use, high sensitivity, and it can distinguish the disease course to achieve precise treatment. These features make it an ideal solution for large-scale brucellosis testing in remote and nomadic areas, where it can play a crucial role in improving public health protection.
研究领域:
医学诊断
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生物传感技术
·
传染病研究
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2025
·Vol. 25
·期 5
·1630
·
IF 3.5
Qiang Xu,Huiting Yin,Mei Cui,Renliang Huang,Rongxin Su
A fiber-reinforced SPR sensor based on silver-nucleated gold-shell bimetallic nanoparticles and graphene oxide was developed and applied to human IgG detection. The refractive index (RI) sensitivity of the Ag@Au/GO fiber SPR sensor is as high as 4715.9 nm/RIU in the RI range of 1.333–1.365. Staphylococcus aureus protein A (SPA) can specifically recognize and bind to the fragment crystallizable (Fc) of the antibody; it facilitates the highly targeted immobilization of the antibody. SPA and rabbit anti-human IgG were immobilized on the surface of the Ag@Au/GO fiber SPR sensor for the detection of different concentrations of human IgG with a sensitivity of 0.53 nm/μg/mL and detection limits of 0.037 μg/mL. This biosensor based on the mixed structure of GO and Ag@Au combined the common advantages of the two materials. Therefore, our study provides a simple platform for biological analysis and has a good application prospect.
研究领域:
生物传感技术
·
材料科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Xu-mei Wang,Yun-hua Ye,Zong-cai Tu,Yue-ming Hu,Hui Wang,Tao Huang
Glycation between proteins and reducing sugars is the common chemical modification in food protein, and many studies have focused on the allergenicity of the glycated protein. However, a systemic study on the allergenicity change of its digests is lacking. In this work, we explored the change rule of the digestibility and allergenicity of glycated β-Lg during invitro gastrointestinal digestion and interpreted the mechanism using high-resolution mass spectrometry. Glycation with arabinose increased the resistance of β-Lg to digestive enzyme, with a low hydrolysis value. Indirect competitive ELISA showed that the IgG/IgE binding rates of β-Lg were reduced after glycation and further reduced after digestion, in comparison with the digests of unglycated β-Lg. There are two reasons for this phenomenon. On the one hand, 11 glycated sites were determined in the lowest allergenicity arabinose-β-Lg conjugation (Ara-β-Lg), which was distributed in the IgG and IgE linear allergic epitopes of β-Lg. On the other hand, glycation masking linear allergenic epitopes had a more significant effect on reducing allergenicity in comparison to digestive enzyme hydrolysis. These results indicated that the allergenicity of Ara-β-Lg in the human body might be lower than that of unglycated β-Lg.
研究领域:
食品科学
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过敏反应
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蛋白质化学
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质谱分析
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Jing-jing Zhang,Zong-cai Tu,Hui Wang,Yue-ming Hu,Peng-cheng Du,Yi-peng Yang
In this work, the mechanism of the effect of lipid oxidation on the IgG/IgE binding ability of ovalbumin (OVA) was investigated via the peroxyl radicals produced by 2, 2′-azobis (2-amidinopropane) dihydrochloride to simulate lipid oxidation. Results showed that the structure of OVA unfolded partially with an increase in oxidation degree, leading to the exposure of the allergenic epitopes and increasing the IgG/IgE binding ability of OVA. Nine oxidation sites were found on the α-helix, and these sites may unwind the α-helix and expose the allergenic epitopes on the OVA surface, leading to antibody recognition and combination. Consequently, the IgG/IgE binding ability of OVA was increased. In conclusion, the allergenic capacity of OVA can be promoted by modifying peroxyl radical oxidation in processing egg products.
研究领域:
食品科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2020
·
IF 3.943
Ningchao Du,Li Song,Yang Li,Tingting Wang,Qinghua Fang,Jiaxin Ou,Kutty Selva Nandakumar
Based on previous studies, we know that estrogen can protect the joints from arthritis development by increasing IgG glycosylation and inhibiting osteoclast activation. Phytoestrogens, especially genistein and daidzein, are structurally similar to estradiol that can bind to estrogen receptors (ERs). However, how phytoestrogens affect IgG glycosylation and osteoclast activation in vivo are not investigated so far. In this study, we used 20 mg/kg genistein or daidzein to gavage the female DBA1/J mice in collagen induced arthritis (CIA). We assessed arthritis and bone erosion by clinical scores, histopathology, and micro-CT analysis. Inflammatory cells such as neutrophils, B cells, macrophages and T cells in the peripheral blood were analyzed by flow cytometry. Phagocytic function of peritoneal macrophages was assessed by using FITC-labeled Escherichia coli . New monoclonal antibodies against CII were produced, purified and analyzed. Glycosylation levels of polyclonal and monoclonal IgG were detected by lectin-ELISA. Quantitative PCR was used to analyze the genes related to IgG glycosylation ( B4galt1, St6gal1) and osteoclasts ( TRAP, NFATC1, c-Fos) . Expression of NF-κB and Akt signaling pathways as well as downstream transcription factors NFATc1 and c-Fos was studied by Western blot. Our results show that phytoestrogens protect mice from CIA by increasing IgG glycosylation leading to amelioration of inflammation and inhibiting the NF-κB pathway and NFATc1/c-Fos to decrease the activity of osteoclasts. In conclusion, phytoestrogens can protect bone and joints in CIA mice by increasing IgG glycosylation and inhibiting osteoclast activity.
研究领域:
免疫学
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风湿病学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 24
·10850-10856
·
IF 3.591
Xiaoyan Chen,Lin Song,Guanyang Zhu,Jing Ma,Ajing Xu,Wenya Zhao,Yingying Gu,Yarui An,Yuqing Miao
In this work, a unique zirconium dioxide solid superacid (ZrO2/SO42−) was utilized for the fabrication of an IgG electrochemical immunosensor. The treated ZrO2 formed a SO bond on the surface, which has the electron-attracting induction effect and can further enhance the quantity and stability of immobilized biomolecules. In addition, using a platinum nanoparticle–chitosan–reduced graphene oxide (PtNP–CHIT–rGO) nanocomposite as a substrate modification material not only improves the conductivity of the electrode, but also increases the effective surface area of the immunosensing platform. The proposed label-free immunosensor has a lower detection limit of 11.0 pg mL−1 and a wide linear range of 0.01–10.0 ng mL−1 and 10.0–4000.0 ng mL−1. Importantly, the immunosensor exhibits good selectivity, excellent stability and reproducibility, and is expected to be applied to the detection of other biomolecules.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Qiao Liu,Xing-Li Xie,Chang-Jie Mao,Jing-Shuai Chen,He-Lin Niu,Ji-Ming Song
A highly selective DNA-based electrochemiluminescence (ECL) based biosensor is described for the detection of human IgG. It is exploiting the effect of steric hindrance that affects the strength of the ECL signal in the presence of IgG. Digoxin-linked signaling DNA was specifically bound to IgG, and this causes steric hindrance which limits the ability of DNA to hybridize with capturing DNA attached to a gold electrode. Europium (II) doped CdSe quantum dots were covalently linked to the DNA in order to generate the ECL signal. Using this steric hindrance hybridization method, the ECL signal of the biosensor were proportional to the concentration of IgG with a wide linear range and a 14 pM detection limit. Conceivably, the method can be expanded to the detection of a wide range of proteins for which homologous recognition elements are available.
研究领域:
生物化学
·
分析化学
·
生物传感器
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Science and Human Wellness
·
2024
·
IF 5.6
Jihua Mao,Yanhong Shao,Hui Wang,Jun Liu,Zongcai Tu
Gut microbiota plays an important role in food allergy. The immunoglobulin G (IgG)/immunoglobulin E (IgE) binding capacity and human gut microbiota changes of digestion products derived from glycated ovalbumin (OVA) were investigated. Gastrointestinal digestion effectively destroyed the primary structure of glycated OVA, resulting in a significantly higher digestibility than gastric digestion, and more abundant peptides < 3 kDa. Moreover, gastric and gastrointestinal digestion products have different fluorescence quenching and red shift of fluorescence peaks, and possess different conformational structures. These changes resulted in a decrease in 28.7 % of the IgE binding capacity of gastrointestinal digestion products beyond that of pepsin. Moreover, gastrointestinal digestion products of glycated OVA increased significantly the proportion of Subdoligranulum , Collinsella , and Bifidobacterium . Therefore, gastrointestinal digestion products of glycated OVA altered human intestinal microbiota, reducing the risk of potential allergy.
研究领域:
免疫学
·
微生物学
·
食品科学
·
过敏研究
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Ju Jian,Zhang Xinyu,Li Lin,Regmi Sagar,Yang Guoqiang,Tang Shixing
Understanding the dynamic changes in antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for evaluating the effectiveness of the vaccine and the stage for the recovery of the COVID-19 disease. A rapid and accurate method for the detection of SARS-CoV-2-specific antibodies is still urgently needed. Here, we developed a novel fluorescent lateral flow immunoassay (LFA) platform for the detection of SARS-CoV-2-specific IgM and IgG by the aggregation-induced emission carbon dots conjugated with the SARS-CoV-2 spike protein (SSP). The aggregation-induced emission carbon dots (AIE-CDs) are one of the best prospect fluorescent probe materials for exhibiting high emission efficiency in both aggregate and solid states. The AIE-CDs were synthesized and displayed dual fluorescence emission, which provides a new perspective for the design of a high sensitivity testing system. In this work, the novel LFA platform adopted the AIE carbon dots, which are used to detect SARS-CoV-2-specific IgM and IgG conveniently. Furthermore, this sensor had a low LOD of 100 pg/ml. Therefore, this newly developed strategy has potential applications in the areas of public health for the advancement of clinical research.
研究领域:
医学诊断
·
生物化学
·
纳米技术
·
公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 7
·2355-2362
·
IF 4.478
Zhao Xueqi,Zhang Yue,Wang Xinghua,Ma Pinyi,Song Daqian,Sun Ying
Long-range surface plasmon resonance (LRSPR) has demonstrated excellent performance in sensing and detection, due to its higher accuracy and sensitivity compared with conventional surface plasmon resonance (cSPR). In this work, we establish an LRSPR biosensor which employs PDA/Ti 3 C 2 -MXene/PDA-gold film as a sensing substrate and gold nanoparticles (AuNPs) as enhancers. Ti 3 C 2 -MXene is an emerging two-dimensional (2D) layered material which is used extensively in immunoassay and biosensing. The sensing substrate comprises two polydopamine (PDA) films between which is sandwiched a Ti 3 C 2 -MXene film based on a gold film, which provides a large surface area and abundant binding sites to rabbit anti-human IgG (Ab 1 ). Sandwich amplification is adopted to enhance the sensitivity of the LRSPR biosensor, and AuNPs/staphylococcal protein A (SPA)/mouse anti-human IgG (Ab 2 ) composites are introduced into the flow cell as enhancers after the immune binding of human IgG to Ab 1 . The antigen (human IgG) detection range is 0.075 μg mL −1 to 40 μg mL −1 , and the limit of detection is almost 20 times lower than that for cSPR biosensors. This novel LRSPR biosensor demonstrates excellent performance in immune sensing over a broad detection range and a low limit of detection. Subsequent modification of the LRSPR sensing platform could be made for extensive application in various biological detection fields. Graphical abstract
研究领域:
生物传感
·
分析化学
·
纳米技术
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）