INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2021
·
IF 5.875
Tianming Cui,Shukai Li,Shengfu Chen,Ying Liang,Haotian Sun,Longgang Wang
Poly(amido amine) dendrimers and indocyanine green have inevitable interaction with proteins and cells, which induces biological toxicity and reduces therapeutic efficacy in vivo . To overcome these shortcomings, a new drug delivery system G5MEK7C(n)-ICG with a “stealth” layer was prepared. The surface of G5MEK7C(n)-ICG was modified with double-layer super hydrophilic zwitterionic materials. In the “stealth” double-layer structure, the outer layer was consisted of zwitterionic Glu-Lys-Glu-Lys-Glu-Lys-Cys (EK7) peptide, and the inner layer was composed of amino and carboxyl groups with a ratio of 1:1. DLS results showed that the average hydrodynamic size of G5MEK7C(n)-ICG was about 25–30 nm, and the zeta potential was proven to undergo a slight charge reversal with the increasing pH values of solutions. Furthermore, G5MEK7C(n)-ICG exhibited excellent biocompatibility to red blood cells and proteins resistance. Photothermal and photodynamic experiments demonstrated that G5MEK7C(n)-ICG had a good photothermal conversion effect and generated singlet oxygen ( 1 O 2 ) under laser irradiation. The MTT and hemolysis results showed that the toxicity of G5 PAMAM was significantly reduced after modification double-layer structure. Cytotoxicity studies and flow cytometry showed G5MEK7C(70)-ICG under laser irradiation had a good effect on killing A549 cells. More importantly, the tumor inhibition rate of mice treated with G5MEK7C(70)-ICG (under laser irradiation) was 78.2% in vivo , which was higher than that of mice treated with free ICG. Compared with free ICG, G5MEK7C(70)-ICG caused less damage to the liver according to the enzyme activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Therefore, dendrimers modified with a zwitterionic double layer will be a promising candidate as a drug delivery system.
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高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 4
·期 1
·105-112
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IF 4.726
Lu Han,Yang Zhang,Xu-Wei Chen,Yang Shu,Jian-Hua Wang
A novel phototherapy nanoplatform is prepared by coating hollow CuS nanoparticles with a bovine serum albumin–folic acid (BSA–FA) complex. The obtained CuS–BSA–FA nanoparticles are used as drug-delivery vehicles to transport a near-infrared-absorbing phototherapeutic agent (indocyanine green, ICG) into HeLa cells, after loading ICG onto CuS–BSA–FA. In this manner, a combined therapy approach is established consisting of photothermal therapy (by CuS–BSA–FA nanocarriers) and cytotoxic effects of photodynamic and photothermal therapy (by ICG upon 808 nm laser irradiation). The encapsulation of ICG onto CuS–BSA–FA significantly improves the stability and reduces the dark toxicity of free ICG. This therapeutic system exhibits an obviously higher photothermal heating effect and capability of 1O2 generation under laser irradiation compared with bare nanocarriers. In addition, the grafted FA segments on the surface of CuS–BSA–FA are proved to enhance the internalization of nanoparticles by FA-receptor-mediated endocytosis.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2024
·Vol. 26
·期 5
·1-16
·
IF 2.1
Tang Xiaohui,Zhang Qian,Aikelamu Kadireya,Bai Jingya,Ma Rong,Wang Mei,Liu Chao
The objective of the study is to prepare quaternized chitosan magnetic nanoparticles (ICG@Fe 3 O 4 @QCS) loaded with indocyanine green and to investigate their properties. Fe 3 O 4 @QCS nanoparticles were prepared by a one-part precipitation method and evaluated for their structure, particle size, morphology, and magnetic responsiveness. ICG@Fe 3 O 4 @QCS nanoparticles were prepared by electrostatic adsorption to investigate the properties of ICG@Fe 3 O 4 @QCS nanoparticles, such as particle size, zeta potential, encapsulation rate, drug loading, stability, photo- and thermo-conversion efficiencies, degree of release, biosafety, and in vitro antitumor property. Fe 3 O 4 @QCS nanoparticles were successfully prepared in the form of short rods with particle sizes around 20–30 nm, positively charged, with basic Fe 3 O 4 skeleton, and possessing specific magnetic responsiveness. ICG@Fe 3 O 4 @QCS nanoparticles were successfully prepared, particle size is around 100–150 nm, charged positively, the encapsulation rate is ≥ 90%, the drug loading is 0.05–2.00%, the release is slower compared with the ICG solution, and hemolysis rate is less than 2% in all cases; in the laser irradiation, the nanoparticles can produce ROS and thermal effects and have the function of NIR imaging. Antitumor experiments in vitro showed that ICG@Fe 3 O 4 @QCS nanoparticles had strong phototoxicity, had a particular killing effect on tumor cells, and could promote Hela cell apoptosis. ICG@Fe 3 O 4 @QCS nanoparticles can be successfully prepared by the electrostatic adsorption method, with the stable nature of ICG@Fe 3 O 4 @QCS nanoparticles, which can improve the bioavailability of ICG, and the prepared nanoparticles have good antitumor properties.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Yalin Ran,Junfeng Hu,Yuanyuan Chen,Zhenan Rao,Jichun Zhao,Zhigang Xu,Jian Ming
Nanoscale drug delivery systems derived from natural bioactive materials accelerate the innovation and evolution of cancer treatment modalities. Morusin (Mor) is a prenylated flavonoid compound with high cancer chemoprevention activity, however, the poor water solubility, low active pharmaceutical ingredient (API) loading content, and instability compromise its bioavailability and therapeutic effectiveness. Herein, a full-API carrier-free nanoparticle is developed based on the self-assembly of indocyanine green (ICG), copper ions (Cu 2+ ) and Mor, termed as IMCNs, via coordination-driven and π-π stacking for synergistic tumor therapy. The IMCNs exhibits a desirable loading content of Mor (58.7 %) and pH/glutathione (GSH)-responsive motif. Moreover, the photothermal stability and photo-heat conversion efficiency (42.8 %) of IMCNs are improved after coordination with Cu 2+ and help to achieve photothermal therapy. Afterward, the released Cu 2+ depletes intracellular overexpressed GSH and mediates Fenton-like reactions, and further synergizes with ICG at high temperatures to expand oxidative damage. Furthermore, the released Mor elicits cytoplasmic vacuolation, expedites mitochondrial dysfunction, and exerts chemo-photothermal therapy after being combined with ICG to suppress the migration of residual live tumor cells. In vivo experiments demonstrate that IMCNs under laser irradiation could excellently inhibit tumor growth (89.6 %) through the multi-modal therapeutic performance of self-enhanced chemotherapy/coordinated-drugs/ photothermal therapy (PTT), presenting a great potential for cancer therapy.
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Advanced Healthcare Materials
·
2024
·
IF 10
Shuairu Zhu,Hongwei Xu,Wanying Guo,Mei Yang,Huiling Tan,Shaosong Hou,Jie Yao,Han Luo,Yongchao Yao,Jingxiang Zhao,Yonggang Wei,Xuping Sun,Binwu Ying
The versatile element composition and multifunctional properties of biodegradable silicates have attracted significant attention in cancer therapeutics. However, their application as nanozymes is often limited by suboptimal catalytic efficiency and insufficient intratumoral retention. In this study, the hydrothermal synthesis of iron silicate (FeSi) nanosheets are reported exhibiting exceptional peroxidase (POD)-like activity (136.7 U mg −1 ), outperforming most reported iron-based nanozymes. Density functional theory calculations revealed that the introduction of Si into the catalyst enhances H 2 O 2 adsorption and dissociation of Fe sites, leading to superior POD performance. Furthermore, the FeSi nanosheets are modified with Indocyanine Green (ICG) to facilitate targeted aggregation-potentiated therapy. The integration of ICG improved tumor penetration and retention of the FeSi nanosheets, significantly increasing their reactive oxygen species production and bolstering therapeutic efficacy.
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DYES AND PIGMENTS
·
2022
·
IF 5.122
Huimin Fan,Shuang Chen,Zhong Du,Ting Yan,Gulinigaer Alimu,Lijun Zhu,Rong Ma,Nuernisha Alifu,Xueliang Zhang
Photothermal therapy (PTT) is a promising and non-invasive treatment for cancer. As essential factors for PTT, photothermal agents (PTAs) have attracted widespread attention. However, high-efficient and biocompatible fluorescent PTAs are still not enough. In this study, new indocyanine green (IR820) was utilized as a fluorescence agent and a PTA on HeLa cells and subcutaneous cervical cancer models of mouse. To improve PTT effect and biocompatibility of IR820, the amphiphilic polymer Pluronic F-127 (F-127) was utilized to modify free IR820 molecules, to form [email protected] nanoparticles (NPs) by self-assembly method. Fortunately, [email protected] NPs exhibited desirable photothermal properties with high photothermal conversion efficiency of 35.2% and good photothermal stability. Cytotoxicity assay, in vitro and in vivo fluorescence imaging of [email protected] NPs exhibited satisfactory biocompatibility, excellent cellular uptake ability, and bioimaging capability on tumor sites. Meanwhile, [email protected] NPs owned cellular-therapy ability against cervical cancer under 793 nm laser irradiation observed in apoptosis assay. Furthermore, the excellent PTT effect (∼90%) in flow cytometry analysis endowed [email protected] NPs with the ability to ablate subcutaneous cervical tumors on nude mice . The newly formed [email protected] NPs have great potential for anti-tumor therapy and bioimaging of cervical cancer, which lays the foundation for the future development of biomedical applications .
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Biomaterials Science
·
2025
·
IF 5.7
Yingao Ma,Jingyu Xiao,Gina Jinna Chen,Hong Dang,Yaran Zhang,Xiaoqin He,Perry Ping Shum,Qiongyu Guo
Photothermal treatment has garnered immense interest as a promising approach for biomedical applications such as cancer ablation, yet its effectiveness is often limited by insufficient laser penetration and challenges in achieving efficient targeting of photothermal agents. Here we developed a transvascular interventional photothermal therapy (Ti-PTT), which employed a small-sized microcatheter (outer diameter: 0.63 mm, 1.9 Fr) equipped with an ultrafine optical fiber (diameter: 100 m) capable of simultaneously delivering photothermal agents while performing 808 nm laser irradiation via an endovascular route. Specifically, we employed two types of indocyanine green (ICG)-based photothermal agent, i.e. ICG solution serving as purely photothermal agent and ICG-ethiodized oil (ICG-EO) emulsion acting as radiopaque photothermal embolic agent. Using the customized microcatheter with the ICG solution, both proximal and distal embolization were able to executed in a rat liver model. Compared to the ICG solution, the ICG-EO emulsion dramatically enhanced the ICG retention time, enabling photothermally triggered precision vascular blockade to induce local embolization of large tissue volumes in a rat kidney model with unfavored ICG leakage rate. The Ti-PTT paves a way to broaden the potential applications of photothermal therapy through combining with clinical intervention-based approaches.
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Journal of Biomedical Nanotechnology
·
2015
·Vol. 11
·期 4
·600-612
·
IF 5.338
Luo Teng,Qian Xiaoqing,Lu Zhiyong,Shi Yiwen,Yao Zhirong,Chai Xinyu,Ren Qiushi
A hydrophilic indocyanine green derivative (ICG-Der-02) was covalently doped into mesoporous silica-coated gold nanorods (AuNRs/mSiO2). The self-synthesized derivative offers one carboxyl functional group on a side chain, which enables ICG-Der-02 to be covalently linked to
nanomaterials and reduces the probability of leakage/desorption of the dye. The detection of infrared luminescence around 1270 nm confirmed that 1O2 is efficiently generated by the nanocomposite (AuNRs/mSiO2-ICG-Der-02). Furthermore, a second layer of silica
was coated onto the nanocomposite, which then was conjugated with the αv
integrin-targeting cyclic peptide (RGD-4C). The cell tests showed that the resulting nanoconjugate (AuNRs/mSiO2-ICG-Der-02/RGD-4C) was able to bind preferentially to HT-1080 human fibrosarcoma
cells. Due to the synergistic effect of the produced nanoconjugates, a dual-modality photothermal and photochemical therapy was successfully achieved by 808 nm irradiation. Compared to using photothermal or photochemical therapy alone, the dualmodality photothermal/photochemical therapeutic
strategy proved to be more damaging to HT-1080 cells and enhanced the effectiveness of photodestruction. Our work presents a novel approach to the multimodal treatment of fibrosarcoma and shows promise for future use in cancer theranostics.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Tao Wang,Heyu He,Zhiling Chen,Ziming Wei,Yang Li,Fengling Li,Dan Xu,Rui Zhang,Chuanzhu Lv,Qiang Wu
Bacterial infections, exacerbated by rising antibiotic resistance, urgently demand innovative antimicrobial strategies. However, conventional indocyanine green (ICG)-based phototherapies are limited by poor photostability, rapid degradation, and nonspecific distribution, which hinder their clinical translation. In this study, we developed a multifunctional nanoplatform integrating photodynamic therapy (PDT) and photothermal therapy (PTT), using hollow bismuth selenide (Bi 2 Se 3 , BS) nano-spherical sponges to encapsulate ICG. The resulting BS/ICG (BSI) hybrid leverages the high surface area and photothermal properties of BS to enhance ICG loading capacity (82.28 % ± 3.42 %) and enable controlled release under near-infrared (NIR) irradiation. Upon 808 nm light activation, the BSI system generates abundant reactive oxygen species (ROS) and induces localized hyperthermia, synergistically disrupting bacterial membranes and biofilms. In vitro experiments demonstrated that the bactericidal efficiency of this platform achieved 99.01 % ± 0.37 % against Staphylococcus aureus and 99.10 % ± 0.28 % against Escherichia coli within just 5 min. Mechanistic studies revealed that ROS-induced oxidative damage and thermal lysis act synergistically on bacterial membrane components, while biofilm formation is inhibited through downregulation of biofilm-associated genes, effectively reducing the risk of bacterial resistance. In an infected mouse wound model, the BSI system significantly accelerated wound healing, promoted neovascularization, suppressed inflammatory responses, and enhanced collagen deposition. Both in vitro and in vivo biocompatibility assessments confirmed the excellent biosafety of the system. This work introduces a promising dual-modal nanotherapeutic platform based on PDT/PTT, offering a valuable alternative for combating multidrug-resistant infections and promoting tissue regeneration.
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Liang Yu,Yan Peng,Linping Jiang,Liyan Qiu
Indocyanine green (ICG) has been widely explored for the theranostics of tumors. However, ICG mainly accumulates in the liver, spleen, or kidney in addition to in tumors, causing inaccurate diagnoses and impaired therapeutic effects under NIR irradiation. Herein, a hybrid nanomicelle was constructed by integrating hypoxia-sensitive iridium(III) and ICG for precise tumor localization and photothermal therapy in sequence. In this nanomicelle, the amphiphilic iridium(III) complex (BTPH)2Ir(SA-PEG) was synthesized through the coordination substitution of hydrophobic (BTPH)2IrCl2 and hydrophilic PEGlyated succinylacetone (SA-PEG). Meanwhile, PEGlyated ICG (ICG-PEG) as a derivative of the photosensitizer ICG was also synthesized. (BTPH)2Ir(SA-PEG) and ICG-PEG were coassembled by dialysis to form the hybrid nanomicelle M-Ir-ICG. Hypoxia-sensitive fluorescence, ROS generation, and the photothermal effect of M-Ir-ICG were investigated in vitro and in vivo. The experimental results indicated that M-Ir-ICG nanomicelles could locate at the tumor site first and then perform photothermal therapy with 83.90% TIR, demonstrating great potential for clinical applications.
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Biomaterials Science
·
2019
·Vol. 7
·期 4
·1705-1715
·
IF 5.251
Chanjuan Liu,Changping Ruan,Rui Shi,Bang-Ping Jiang,Shichen Ji,Xing-Can Shen
Indocyanine green (ICG), a multifunctional near-infrared (NIR) imaging agent approved by the FDA, has been extensively used in clinical cancer theranosis, but limited by its inherent instability, short plasma half-life and lack of targeting ability. Herein, an in situ formed photothermal network based thermosensitive hydrogel (PNT-gel) constructed by using supramolecular cross-linking conjugated polymers was developed for the stabilization of ICG and efficient combinatorial photothermal/photodynamic antitumor therapy. While the conjugated polymeric backbone in PNT-gel anchored the aromatic phototherapeutic agent ICG via π–π stacking interactions to avoid premature leakage, it also directly converted low-dose NIR light to induce localized hyperthermia to enhance the photothermal effect. The PNT-gel shows a reversible gel-to-sol upper critical solution temperature (UCST) that is slightly above body temperature. Therefore, the controlled release of ICG was switched on or off by NIR via photothermal-induced gel–sol transition. In vitro and in vivo antitumor experiments demonstrated that ICG loaded PNT-gel not only efficiently induced the killing of 4T1 cancer cells, but also achieved almost complete eradication of 4T1 cells by one-dose intratumoral injection in combinatorial photothermal/photodynamic therapy under irradiation of a low-dose 808 nm laser (0.14 W cm−2). Additionally, the combinational therapy proved to enhance the effectiveness of photodestruction without tumor recurrence compared with photothermal therapy (PTT) or photodynamic therapy (PDT) treatment alone.
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Materials Today Nano
·
2018
·
IF 0
K. Jiang,L. Zhang,Q. Hu,D. Yue,J. Zhang,X. Zhang,B. Li,Y. Cui,Y. Yang,G. Qian
Indocyanine green (ICG), as the only U.S. Food and Drug Administration–approved near-infrared (NIR) clinical agent, has been considered as an ideal light absorber for laser-mediated photothermal therapy (PTT) in cancer treatment. However, the practical applications of ICG are severely hampered by its poor aqueous stability, rapid body clearance, and low cellular uptake. To overcome these limitations, we herein report the successful example of integrating ICG into a zeolitic imidazolate framework (ZIF-8) to fabricate a novel nanoscale ICG@ZIF-8 hybrid material . Through a simple one-pot synthesis method, a high loading content of 20.6% can be achieved in the resultant ICG@ZIF-8. The photostability and tumor accumulation of ICG are notably promoted due to the protection of the framework, leading to enhanced photothermal conversion efficiency. Furthermore, we also discover, for the first time, that the pH-triggered release of large amount of Zn 2+ from ZIF-8 in tumor acidic microenvironment also significantly contributes to targeted killing of cancer cells. As a result of the combined PTT and chemotherapy, ICG@ZIF-8 exhibits greatly improved diagnostic efficacy for both in vitro and in vivo cancer therapy, leading to 91% tumor eradication in all the mice treated with ICG@ZIF-8 and NIR irradiation. Hematoxylin and eosin (H&E)–stained slices show that no noticeable tissue damage is observed in major organs, indicating the safety of ICG@ZIF-8.
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