BMC BIOLOGY
·
2025
·Vol. 23
·期 1
·1-15
·
IF 4.5
Liu Xinfeng,Wang Tongcheng,Zheng Zeyu,Dong Congcong,Liu Wenyu,Niu Zhimin,Yang Yingbo,Wu Xiaoyun,Qiu Qiang,Yang Yongzhi,Li Kexin,Lenstra Johannes A.,Yan Ping,Kang Minghui,Li Bowen,Fang Lingzhao,Liu Jianquan
Background Animal pigmentation serves as an excellent model for studying genetics, development, and evolution. Among yak breeds, the all-white yak breed ( Bos grunniens ) is the only indigenous variety with pristine white fur, in stark contrast to the black coat color of the wild yak and most domesticated yaks. Results Using whole-genome sequencing data from 387 yaks, we analyzed the population genetic structure of all-white yaks and discovered that they clustered into two distinct genetic groups. Further, by conducting a genome-wide association study (GWAS) based on whole-genome variants (SNPs and indels) between all-white and wild-type yaks, we identified a 14-bp deletion in the promoter of KIT , which decreased its expression in all-white yaks. The following knock-in experiments in mouse confirmed that the absence of the yak 14-bp motif decreases the expression of KIT . Deletion of a human orthologue of the yak 14-bp motif by using the CRISPR/Cas9 system reduces the melanin accumulation in human melanoma cells. Conclusions Overall, our study revealed the genetic basis of all-white yaks and underscored the importance of studying livestock phenotypes to uncover conserved genetic regulators in mammals.
研究领域:
遗传学
·
发育生物学
·
进化生物学
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2015
·Vol. 39
·期 10
·7971-7978
·
IF 3.086
Huan Liu,Kai Tao,Peipei Zhang,Wei Xu,Shenghu Zhou
Ordered tungsten and aluminum co-doped mesoporous KIT-6 catalysts (W-Al-KIT-6) with different Si/Al molar ratios were successfully synthesized by a one-pot synthesis method. The obtained W-Al-KIT-6 catalysts were tested for catalytic conversion of 1-butene and ethene to propene via isomerization of 1-butene to 2-butene and subsequent cross metathesis of 2-butene and ethene. Various characterization techniques, such as ICP-OES, XRD, BET, TEM, Raman, XPS and NH3-TPD, were used to characterize the catalysts. The introduction of Al did not change the mesoporous structure of KIT-6 when the nominal Si/Al was 10, 20 or 30. Moreover, the sample demonstrated a larger amount of acidic sites. The W-Al-KIT-6 catalysts with suitable Si/Al ratios illustrated a superior catalytic performance to W-KIT-6 catalyst. The origin of catalytic performance enhancement over W-Al-KIT-6 catalysts is preliminarily discussed and ascribed to the highly disperse W species and a large amount of acidic sites. The acidic sites were formed by the introduction of a suitable amount of Al in the W-KIT-6 framework, which accelerated the isomerization of 1-butene to 2-butene and promoted the cross metathesis of 2-butene and ethene to propene.
研究领域:
材料科学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 9
·6932-6938
·
IF 3.289
Yueyun Li,Yihe Zhang,Liping Jiang,Paul K. Chu,Ynhui Dong,Ping Wang
An ultrasensitive electrochemical immunosensor is designed and constructed for the quantitative detection of squamous cell carcinoma antigens (SCCAs). Silver nanoparticles (AgNPs) hybridized and 3-aminopropyltriethoxysilane functionalized graphene sheets (Ag@APTES-GS) serve as the platform to immobilize primary antibodies (Ab1). The mesoporous AgNPs-decorated silica KIT-6 (Ag@KIT-6) with a large specific surface area is utilized to label the electron mediator thionin (TH) and secondary antibodies (Ab2) by physical adsorption and chemical bonding. Carboxymethyl chitosan (CMC) doped with ionic liquids (ILs) is used to facilitate electron transfer and prevent leaking of TH resulting in multiple signal amplification. In addition to the excellent detection limit (17 fg mL−1, S/N = 3) and wide linear range (5 × 10−5 ng mL−1 to 102 ng mL−1), the immunosensor shows good selectivity, reproducibility, and stability. Providing quantitative detection of SCCA in serum, the sensor has great potential in clinical and diagnostic applications.
研究领域:
生物医学工程
·
诊断学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2017
·
IF 4.601
Sainan Wei,Huijun He,Yan Cheng,Chunping Yang,Guangming Zeng,Li Kang,Hui Qian,Canyao Zhu
Sulfur-containing compounds in oils are harmful to the ecosystem whose cost-effective removal is still a challenge. In this study, novel heterogeneous catalysts of cobalt supported on KIT-6 mesoporous silicas were prepared and evaluated for oxidative desulfurization (ODS) under ambient conditions using cyclohexanone peroxide (CYHPO) as the oxidant. The model oil was obtained via dissolving DBT in the n-octane. The conventional wet impregnation method was applied for the preparation of the catalysts, and cobalt nitrate was used as the precursor. The catalysts were characterized by XRD, N 2 -physisorption, FT-IR, TGA, SEM/TEM and XPS. Then their catalytic activities in total oxidation of dibenzothiophene (DBT) were evaluated at various conditions. DBT conversion by this heterogeneous ODS system reached 98.68%. Co/KIT-6 catalysts showed high ODS activity due to Ia3d mesoporous structure resulting in better cobalt dispersion, higher activity of Co, and faster diffusion of reactant and products. The Box-Behnken design showed that the optimum values for the conversion of DBT were 93 °C (oxidation temperature), 1.6 × 10 −1 g (catalyst amount) and 5.7 (O/S molar ratio), respectively. The inhibitory effect of quinoline on ODS was higher than carbazole and indole, adding quinoline into model oil under same conditions led to a decrease of the conversion of DBT from 98.68% to 65.67% when the concentration of quinoline increased from 160 ppm to 4845 ppm. Kinetics data correlated well with the pseudo-first-order equation. These data showed that this novel Co/KIT-6 catalysis system has the potential to be applied in ODS from oils.
研究领域:
化学工程
·
环境科学
·
催化材料
✓ 已验证
产品关联
DOI
高影响因子
CATALYSIS LETTERS
·
2015
·Vol. 145
·期 3
·784-793
·
IF 2.307
Zhang Xuejun,Zhang Peipei,Yu Hongbo,Ma Zhen,Zhou Shenghu
Abstract Bimetallic PdM (M = Ni, Co, Fe) nanoparticles were synthesized using butyllithium as a reductant, and were used to prepare Pd–M
x
O
y
/KIT-6 catalysts by appropriate oxidation and reduction treatments. These catalysts showed higher selectivity in the hydrogenation of p -chloronitrobenzene to p -chloroaniline than Pd/KIT-6. Relevant characterization was conducted using X-ray diffraction, transmission electron microscopy, N 2 adsorption–desorption, X-ray photoelectron spectroscopy, inductive coupled plasma emission spectrometer, and H 2 temperature-programmed reduction. Graphical Abstract
研究领域:
催化化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Forensic Sciences Research
·
2019
·Vol. 7
·期 2
·172-180
·
IF 0
Tao Ruiyang,Chen Chong,Sheng Xiang,Xia Ruocheng,Zhang Xiaochun,Zhang Jingyi,Yang Zihao,Zhang Suhua,Li Chengtao
The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer mix to check the PCR performance. This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions, sensitivity, precision, accuracy, repeatability, reproducibility, and concordance; tolerance to PCR inhibitors; applicability to real “forensic-type” samples; species specificity; mixture, balance and stutter analyses, and utility in a population investigation. The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate, sensitive and robust for STR genotyping. In addition, these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population.
研究领域:
法医学
·
分子生物学
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
TALANTA
·
2021
·
IF 6.057
Yunhe Yang,Yang Yang,Shuangshuang Wang,Huihui Li,David D.Y. Chen
The formation of G-quadruplex (G4) structures in oncogenic G-rich promoter regions are implicated in their biological functions, especially the inhibition of transcription. The binding of cations is thought to contribute to the stabilization of the G4 formation and competition against the duplex formation in the genomic sequence. Furthermore, it might affect the recognition of DNA-binding proteins. Therefore, measuring the interaction between G4 DNA and cations in a free solution environment is critical for evaluating G4 DNA biological functions. However, how binding to cations (K + and NH 4 + ) affects the folding equilibrium of the G4 structure remains unclear. In this work, a Taylor dispersion analysis (TDA) method using a capillary electrophoresis (CE) instrument was established for the quantitative characterization of the cation-dependent G4 formation in the human c-KIT oncogene promoter region, as well as diffusivities and hydrodynamic radii of DNA variations before and after folding. Our results showed that both K + and NH 4 + can induce the random-coiled c-KIT DNA to unfold and form a more unstretched intermediate state and then fold into tightly structured G4s with smaller size. The G4 size induced by NH 4 + was smaller than that induced by K + ions, though these two cations induced the c-KIT G4 DNA formation with similar binding constants (order of magnitude around 10 6 M −1 ). The TDA method can be widely used for rapid structural analyses of trace amounts of DNA mixtures, which effectively differentiate DNA variations or DNA-ligand complex conformations.
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Zhijie Zhang,Deben Li,Hao Hu,Yaoqing Chu,Jiayue Xu
Metal halide perovskites (MHPs) are emerging as promising candidates for photocatalytic CO2 conversion. However, their practical application is still restricted by the poor intrinsic stability and weak adsorption/activation toward CO2 molecules. The rational design of MHPs-based heterostructures with high stability and abundant active sites is a potential solution to this obstacle. Herein, we report the in situ growth of lead-free Cs2CuBr4 perovskite quantum dots (PQDs) in KIT-6 mesoporous molecular sieve, obtaining remarkable photocatalytic CO2 reduction activity and durable stability. The optimized Cs2CuBr4@KIT-6 heterostructure exhibits the photocatalytic CO and CH4 evolution rates of 51.6 and 17.2 μmol g–1 h–1, respectively, far exceeding those of pristine Cs2CuBr4. On the basis of in situ diffuse reflectance infrared Fourier transform spectra and theoretical investigations, the detailed CO2 photoreduction pathway is systematically revealed. This work provides a new route for the rational construction of perovskite-based heterostructures with strong CO2 adsorption/activation and good stability for photocatalytic CO2 reduction.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-9
·
IF 3.8
Yuan Fang-Fang,Wang Pei,Han Xiao-Jie,Qin Ting-Ting,Lu Xin,Bai Hai-Jiao
The microreactor with two types of immobilized enzymes, exhibiting excellent orthogonal performance, represents an effective approach to counteract the reduced digestion efficiency resulting from the absence of a single enzyme cleavage site, thereby impacting protein identification. In this study, we developed a hydrophilic dual-enzyme microreactor characterized by rapid mass transfer and superior enzymatic activity. Initially, we selected KIT-6 molecular sieve as the carrier for the dual-IMER due to its three-dimensional network pore structure. Modification involved co-deposition of polyethyleneimine (PEI) and acrylamide (AM) as amine donors, along with dopamine to enhance material hydrophilicity. Remaining amino and double bond functional groups facilitated stepwise immobilization of trypsin and Glu-C. Digestion times for bovine serum albumin (BSA) and bovine hemoglobin (BHb) on the dual-IMER were significantly reduced compared to solution-based digestion (1 min vs. 36 h), resulting in improved sequence coverage (91.30% vs. 82.7% for BSA; 90.24% vs. 89.20% for BHb). Additionally, the dual-IMER demonstrated excellent durability, retaining 96.08% relative activity after 29 reuse cycles. Enhanced protein digestion efficiency can be attributed to several factors: (1) KIT-6’s large specific surface area, enabling higher enzyme loading capacity; (2) Its three-dimensional network pore structure, facilitating faster mass transfer and substance diffusion; (3) Orthogonality of trypsin and Glu-C enzyme cleavage sites; (4) The spatial effect introduced by the chain structure of PEI and glutaraldehyde’s spacing arm, reducing spatial hindrance and enhancing enzyme–substrate interactions; (5) Mild and stable enzyme immobilization. The KIT-6-based dual-IMER offers a promising technical tool for protein digestion, while the PDA/PEI/AM-KIT-6 platform holds potential for immobilizing other proteins or active substances.
研究领域:
生物化学
·
蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS A-GENERAL
·
2023
·
IF 5.5
Shaoqing Wang,Yan Jiao,Zhihe Li,Zhen Wan,Yu Han,Weiming Yi
In this paper, a surface encapsulation method has been developed to synthesize the HZSM-5 @Al-KIT-6 hierarchical zeolites, due to the poor mono-aromatic hydrocarbons (MAHs) selectivity and rapid coke deactivation during the catalytic upgrading lignin pyrolysis vapors. Results showed that the acidity and accessibility of HZSM-5 @Al-KIT-6 hierarchical zeolite can be modulated by changing the TEOS/HZSM-5 mass ratio during catalyst synthesis. The three-dimensional (3-D) cubic mesoporous shell can capture the bulky phenolic oligomers and pre-crack for further reforming. The highest MAH content (44.03%) and lower polycyclic aromatic hydrocarbons (PAHs) (23.79%) were produced when the TEOS/HZSM-5 mass ratio was 1.0. The hierarchical zeolites improved the yield of xylenes due to the enhanced alkylation reaction. In addition, the micro-mesoporous highway reduced the formation of coke deposition. The catalytic mechanism of hierarchical zeolites was then proposed based on the obtained results. This study provides a reference for pilot-scale experiments for the catalytic upgrading lignin pyrolysis vapors.
研究领域:
化学工程
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Li Gao,Ubong Jerome Etim,Zhiyuan Shi,Wei Xing,Pingping Wu,Dezhi Han,Peng Bai,Zifeng Yan
A Beta-KIT-6 (BK) micro-mesoporous composite material was assembled using zeolite Beta seed solution as raw material. BK composite contained both zeolite Beta units and three-dimensional cubic Ia3d mesostructure of KIT-6, which served as a catalyst support for hydroisomerization of n-heptane. The composite was characterized by a variety of techniques. Results show that catalyst Pt/HBK using the composite as the support exhibited the highest isoheptanes yield among all catalysts, which is attributed to both appropriate surface acidity and excellent mass transfer properties of Beta-KIT-6 composite. Experimental results verified that the indexed isomerization factor as a general performance descriptor could accurately predict the hydroisomerization performance of support materials.
研究领域:
催化化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2023
·
IF 3.4
Guoliang Zhang,Haoyu Liu,Sen Fu,Hong Yuan
BACKGROUND A series of ZSM-5@KIT-6 composite molecular sieve catalysts was prepared by encapsulating ZSM-5 as a substrate in the mesoporous material KIT-6. The intent was to use these catalysts to promote the catalytic cracking of a waste cooking oil model compound (WCOMC) to produce light olefins. RESULTS The morphology, structural characteristics and acidity of each of the resulting catalysts were characterized by X-ray diffraction, transmission electron microscopy, N 2 -Brunauer–Emmett–Teller (N 2 -BET) surface area analysis and temperature-programmed ammonia desorption. The results showed that ZSM-5 was successfully coated with KIT-6. The BET surface areas of these catalysts ranged from 345 to 792 m 2 g −1 and the specimens had average pore sizes in the range of 2.37–5.51 nm. Total acidity increased with increases in the mass-based proportion of ZSM-5 in the material. CONCLUSION The catalytic cracking of WCOMC was studied in a lab-scale fixed-bed apparatus. Trials at a reaction temperature of 600 °C using the material having a mass-based ZSM-5 proportion of 80.0% (ZK-80) gave the highest gas-phase yield of light olefins of 43.8%. After 6000 min, the catalytic performance of this material remained stable, demonstrating a resistance to carbon deposits. The superior activity of the present composite catalysts can be ascribed to improvements in pore structure and acid strength distribution. © 2023 Society of Chemical Industry (SCI).
研究领域:
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）