Cell Reports Physical Science
·
2026
·
IF 6.9
Qi Yi,Yi-Qi Sheng,Dong-Qi Han,Xue-Wei Lin,Chi-Kit Siu,Zhong-Ping Yao
Chiral recognition in mass spectrometry typically relies on one chiral selector (CS) to distinguish enantiomers through dissociation of diastereomeric complexes. Here, we report an innovative approach employing two different CSs to form trimeric complexes with analytes in the presence of Cu 2+ . Nineteen proteinogenic chiral amino acids are investigated as both analytes and selectors, and complexes involving proline and aromatic amino acids exhibit superior chiral discrimination. Notably, the two selectors can play distinct roles: one plays an anchoring role, while the other only contributes to the degree of discrimination. Computational modeling reveals that the copper-bound trimers (e.g., [Cu II (Pro)(Phe)(Tyr) − H] + ) adopt a pentacoordinated geometry with a square-planar base and an elongated axial bond. Three energetically similar precursor structures co-exist, with dissociation occurring exclusively at the axial position. The strong agreement between experimental and computational results validates this binary CS concept and provides a framework for designing advanced CSs for unknown samples.
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JOURNAL OF FOOD PROCESS ENGINEERING
·
2026
·Vol. 49
·期 2
·e70394
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IF 3.5
Shutian Ma,Hongyu Zou,Manling Zhang,Jinyuan Zhang,Zhuang Shi,Yi Yang,Jinglin Zhang,Yuang Zhang,Liming Zhang,Enjie Diao
Black garlic (BG) was prepared at 70°C–75°C with different processing relative humidities (RHs) for 9 days. The effects of processing RH (65%–95%) on the physicochemical properties and antioxidant capacity of BG were investigated. The results showed that higher processing RH was not conducive to the browning of BG. When the processing RH increased from 65% to 95%, the glucose and fructose contents in BG obviously increased by 42.96% and 112.80% ( p < 0.05), respectively; while the browning degree, total acid, total polyphenol, total flavonoid, allicin, and alliin contents decreased by 4.01%, 11.44%, 27.36%, 44.26%, 40.98%, and 48.10% ( p < 0.05), respectively. The potentially harmful substances acrylamide and 5-hydroxymethylfurfural (5-HMF) also significantly decreased by 69.63% and 82.61% ( p < 0.05), respectively. However, the IC 50 values for DPPH and ABTS increased by 28.75% and 46.96% ( p < 0.05), respectively, indicating the decrease in antioxidant capacity of BG. The changes in fructose content and IC 50 values could be well simulated by the zero-order kinetic models, while other physicochemical parameters all followed the pseudo-first-order kinetic models with different regression equations. Overall, 85% processing RH can effectively balance sensory, nutritional, and antioxidant properties of BG, as well as better inhibit the formation of acrylamide and 5-HMF, which can be used as a reference to guide the industrial production of BG for enhancing its quality and safety.
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Food Bioscience
·
2026
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IF 6.2
Yingying Zeng,Weiwei He,Yuhao Li,Junyi Yin,Mingyong Xie
Fermented celery puree is valued for its low caloric content and health benefits. However, its characteristic grassy flavor often limits consumer acceptance. This study examined how supplementation with four amino acids (i.e. valine, leucine, isoleucine, and particularly phenylalanine) modulated the fermentation of celery puree by three lactic acid bacteria (LAB) strains, including Lactiplantibacillus plantarum NCU116, L. casei NCU215, and L. acidophilus NCU402. Among all fermentations, phenylalanine supplementation showed the most pronounced enhancement in both metabolic activity and aroma formation. Amino acid addition accelerated acidification and microbial growth in NCU116 and NCU215 fermentations, whereas its influence on NCU402 was minimal. Phenylalanine supplementation markedly enhanced floral and fruity volatiles, elevating phenylacetaldehyde (∼23-fold), phenylethyl alcohol (∼3-fold), linalool (∼2-fold), and 1,4-dimethyl-4-acetyl-1-cyclohexene (∼1.25-fold). In contrast, valine, isoleucine, and leucine showed only minor effects, mainly on energy metabolism and overall volatile balance. Metabolomic pathway enrichment revealed activation of the phenylpropanoid and shikimate pathways by phenylalanine, indicating enhanced aromatic metabolism, whereas the other amino acids mainly modulated glycolysis/TCA-linked energy metabolism. These findings indicate that phenylalanine acts as an important exogenous precursor and key contributor to aromatic metabolism and volatile formation in NCU215-fermented celery puree, providing mechanistic insights and a practical strategy to enhance sensory acceptance and functional quality of plant-based fermented foods.
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COMPUTERS AND ELECTRONICS IN AGRICULTURE
·
2026
·
IF 10.3
Liru Yan,Bianxiang Zhang,Wei Zhou,Chao Feng,Caihong Zhang,Yan Zhang,Shaomin Shuang,Lihong Shi
Highly-intelligent user-friendly sensing strategies are becoming desperately demanded and indispensable in under-developed nations and resources-insufficient areas. Herein, an intelligent sensing platform has been successfully established incorporating smartphone with machine vision and deep learning function, 3D-printed handheld device, and paper microfluidic chip stained with tri-emission ratiometric-fluorescence (FL) carbon dots (TCDs). The intelligent system can quantify zinc ion (Zn 2+ ) and ethylenediaminetetraacetic acid (EDTA) only through a single-step operation on smartphone. TCDs, engendering bright blue and cyan FL as well as weak red one at 370 nm excitation, were prepared hydrothermally using tartaric acid and alizarin carmine. As-prepared TCDs exhibit continuous FL color transformation from blue to purple to blue with the addition of Zn 2+ and EDTA. Subsequently, TCDs-based paper chip is explored to enable field-portable sequential discrimination of Zn 2+ and EDTA. Intriguingly, the proposed sensor is utilized for fast and intelligent quantification of Zn 2+ and EDTA in soil filtrate, plant sap and tap water with a low detection limit of 3.8 μM and 7.9 μM, as well as wide linear correlations of 0–280 µM and 0–560 µM, respectively. It is eagerly anticipated that our methodologies will become the strong impetus for new round of technological transformation for analyte assay.
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Advanced Sensor and Energy Materials
·
2026
·
IF 12.3
Tingting Wang,Hao Song,Xiaohui Gong,Zongyu Han,Taoming Xu,Yanan Xu,Dan Zhao,Chuanxia Chen,Yizhong Lu,Jian Sun
Biothiol analysis is crucial for evaluating redox homeostasis and monitoring various physiological processes. However, conventional fluorescence-based strategies often rely on structurally complex probes and lack straightforward multi-target discriminative capabilities. Herein, we present a versatile, pre-synthesis-free fluorogenic platform driven by the target-responsive inhibition of in-situ polymer dot (PD) formation. We discovered that dopamine (DA) spontaneously condenses with N -[3-(trimethoxysilyl)propyl]diethylenetriamine (AEEA) to yield robustly fluorescent PDs. Biothiols, acting as highly reactive competitors, intercept the DA precursor via a preferential Michael addition, fundamentally suppressing PD formation and inducing a marked fluorescence decrease. This mechanism enables the rapid, highly sensitive quantification of glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), with low limits of detection (LODs) of 0.095, 0.115, and 0.083 μM, respectively. Significantly, the introduction of a structurally distinct DA-DAMO analog facilitated the discrimination of these biothiols. This reaction-interference strategy was then successfully coupled with enzymatic cascades to evaluate glutathione reductase (GR) activity within 20 min, achieving a LOD of 0.046 mU/mL. The programmable analyte-dependent fluorescence outputs were successfully employed as signal readouts to construct molecular logic gates. This work establishes target-regulated in-situ nanomaterial formation as a powerful paradigm for biothiol sensing, enzyme assays, and advanced logic operations.
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Food Chemistry-X
·
2025
·
IF 8.2
Yong Li,Jingyu Liu,Junlong Meng,Cuiping Feng,Jie Chen,Maomao Zeng,Hua Li
This study investigated the formation of harmful Maillard reaction products (MRPs), particularly advanced glycation end products (AGEs) and 1,2-dicarbonyl compounds, in fried shiitake mushroom crisps prepared from ten types of Lentinula edodes , in relation to their inherent chemical components. Analyzed harmful MRPs included 1,2-dicarbonyls (3-deoxyglucosone/3-DG, glyoxal/GO, methylglyoxal/MGO, diacetyl/DA), hydroxymethylfurfural/HMF, and AGEs (carboxymethyllysine/CML, carboxyethyllysine/CEL, methylglyoxal-derived hydroimidazolone1/MG-H1). Results revealed the positive effect of glucose, 3-DG, Asp, Arg, and Lys on the HMF levels. Conversely, ergothioneine negatively correlated with 3-DG, DA, MGO, CML, and CEL; vanillic acid and caffeic acid were inversely related to GO and MG-H1, respectively. Additionally, Ser positively correlated with CML, while CEL had significant negative correlations with Asp, Gly, and total free amino acid. Overall, there was a complex relationship between the chemical components and harmful MRPs in fried shiitake crisps, with ergothioneine, caffeic acid, and vanillic acid showing potential inhibitors of AGEs and 1,2-dicarbonyls generation.
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PubMed
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Journal of Bioresources and Bioproducts
·
2025
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IF 13
Qian Zhang,Hui’e Jiang,Zhijian Li,Lijuan Chen,Fengqian Yang,Jiamin Zhang,Bo Zhang,Xinhua Liu
Uric acid (UA) level is a pivotal clinical human-health biomarker providing predictive feedback for multitudinous well-known kidney, cardiovascular and metabolic syndrome diseases. Off-the-shelf UA detection methods clinically rely on uricase suffer from limitations such as high costs, longstanding result acquisition, circumscribed testing locations, rigorous expertise requirements, and difficulty in home-detecting due to serum testing systems. Here, inspired by the pH-paper, a scaleable, rapid, non-invasive/-enzymatic/-serodiagnostic, and home-detecting “abnormal UA alarm” platform for UA detection in saliva was developed by strategically integrating the proposed paper-based fluorescent sensing-materials (NIFP-SM) with a user-orientated intelligent red-green-blue (RGB) analysis device. Therefore, NIFP-SM is nano-engineered through straightforward interfacial interactions of functional building blocks of on-demand naphthyl imide-derived fluorescent self-assembled micro-particles (NIFS) with lamellar structure and commercially-used filter paper. The NIFS possesses dominantly wide detection range (0–5 000 µmol/L) and high sensitivity (limit of detection = 0.91 µmol/L). Surprisingly, NIFS exhibited outstanding identifiability for uric acid even in the presence of 16 interferents, substantiating accurate detection-capability in intricate environments. Thus NIFP-SM equipped with NIFS resoundingly achieved efficient, rapid, and on-site visual detection of UA in saliva, urine-simulants, and foods. Further, the NIFP-SM-based automatic analysis platform integrated with an intelligent RGB analysis device was manufactured and enabled accurate quantitative, low-cost, non-invasive/-enzymatic/-serodiagnostic, rapid, home-detecting for UA, eliminating the need for costly equipment and specialized personnel and thereby facilitating early-warning detection of abnormal UA-levels associated diseases.
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JOURNAL OF NUTRITIONAL BIOCHEMISTRY
·
2025
·
IF 4.9
Jianhui Feng,Wenhao Cao,Zhenhua Wang,Bo Xu,Gang Li,Mei Li
Ulcerative colitis, a multifactorial chronic condition of the gastrointestinal tract, has seen increasing interest in dietary intervention as part of its treatment strategy. In this study, we utilized a Dextran Sodium Sulfate (DSS)-induced acute colitis mouse model to assess the impact of leucine and glutamic acid on mucosal ulceration, inflammatory cell infiltration, and proteomic alterations in colon tissue. The results indicated that both glutamic acid and leucine alleviate the symptoms of colitis by reducing the histological scores of mice colon, promoting the expression of anti-inflammatory cytokines (IL-4 and IL-10) and suppressing the expression of pro-inflammatory cytokines (TGF-β and TNF-α). A total of 8576 proteins were detected, with 315 proteins showing significant differences between glutamic acid and model, and with371 proteins showing significant differences between leucine and the model. These common significantly different proteins were mainly enriched with oxidative phosphorylation pathway as well as immunity.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jiangtao Meng,Benjun Yang,Lei Ren,Fengling Yang,Jingkuan Li,Jing Wang
Understanding nitrogen transformation mechanisms during pyrolysis of protein-rich biomass is crucial for biofuel, chemical production, and emission control. Using representative amino acids (Aspartic acid, Histidine, Leucine, and Phenylalanine) spanning polar/non-polar types, we pyrolyzed them at key temperatures to establish molecular conformation‑nitrogen partitioning relationships. Polar amino acids (aspartic acid and histidine) preferentially formed char-N (20–38 wt%) through bimolecular cross-linking at 750 °C. Conversely, non-polar leucine and phenylalanine enriched nitrogen in the tar phase due to side-chain inertness, which simultaneously hindered nitrogen migration to char. Significantly, steric hindrance governed reaction pathway selectivity. In phenylalanine, for instance, the phenyl ring reduced the cyclization barrier by 54.35 kcal/mol versus straight-chain dehydrogenation, facilitating preferential formation of nitrogen-containing chemicals such as indole and phenylacetonitrile via dehydrogenation/decarboxylation reactions in the primary pyrolysis of phenylalanine and secondary cracking of its tar. This work establishes molecular-level theoretical foundations for targeted synthesis of nitrogenous chemicals and NOx precursor emission control during nitrogen-rich biomass pyrolysis.
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Analytical Methods
·
2025
·
IF 2.6
Tianping Chen,Yanru Tao,Xi Cao,Shiyi He,Hua Zhao,Jiangling Hu,Hongmei Wang,Xinhui Jiang
This study developed a high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) method for the simultaneous determination of 19 amino acids in the cortex, hippocampus, and bone marrow samples of BALB/C-nu/nu and NOD/SCID mice. We measured the changes in the levels of 19 amino substances in the cortex, hippocampus, and bone marrow samples of mice in the blank model group, acute leukemia model group, chronic leukemia model group, and treatment group. The chromatographic column used was a COSMOSIL 5 C18-MS-II analytical column (150 mm × 4.6 mm, 5 μm), with the column temperature set at 35 °C. The mobile phase was a methanol-ammonium acetate buffer solution (30 mmol L−1 ammonium acetate solution, adjusted to pH 6.40 with acetic acid), at a flow rate of 0.9 mL min−1. The single analysis time was 46 minutes. By optimizing the analysis conditions, the newly established HPLC-FLD method exhibits high sensitivity, good accuracy, and good reproducibility. This method can be successfully applied to determine 19 amino substances in the cortex, hippocampus, and bone marrow of BALB/C-nu/nu and NOD/SCID mice. The differences in amino acid content between different groups can be used to further study the development process of leukemia, disease diagnostic markers, and personalized treatment plans.
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TALANTA
·
2025
·
IF 6.1
Xiwang Hou,Tianhao Chen,Chunyan Wang,Fanyu Liu,Minggang Zhao,Ye Ma
The concentration range of uric acid (UA) varies significantly across different biological fluids, and therefore, highly sensitive and wide-range detection of UA is of great importance for the early diagnosis of UA-related diseases. Surface-enhanced Raman spectroscopy (SERS) offers high sensitivity and molecular fingerprint specificity; however, challenges remain regarding its signal uniformity and detection range. In this work, a uniform and stable two-dimensional metasurface is constructed via liquid–liquid interface (LLI) self-assembly of silver nanoparticles (NPs) functionalized with bis (p-sulfonatophenyl) phenylphosphine di-potassium salt (BSPP). UA is catalytically oxidized by uricase, which in turn oxidizes the BSPP ligands on the NP surface, inducing microstructural changes in the metasurface, which led to a decrease in the BSPP signal in the SERS mode at low UA concentrations and a reduction in reflectance in the reflectance mode at high UA concentrations. Using this dual-mode strategy, a detection limit (LOD) of 3.22 ± 0.10 nM for UA and a dynamic range of 0.100–1500 μM are achieved. The strategy enables rapid NPs assembly and complementary linear ranges, offering a versatile approach with potential for extension to diverse analytes and portable point-of-care applications, highlighting its potential significance for the early diagnosis of UA–related diseases.
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Sensing and Bio-Sensing Research
·
2025
·
IF 4.9
Jianjun Kang,Xue Lin,Chen Lin,Wuyuan Pan,Jian Wang,Fang Ke
Levodopa (L-DOPA) is a crucial neurotransmitter utilized in the treatment of neurological disorders, particularly in Parkinson's disease, where its concentration in biological fluids serves as a key diagnostic marker. Despite its significance, current methods for L-DOPA detection often suffer from limitations such as prolonged detection times, low sensitivity and weak anti-interference. This study addresses these challenges by introducing a highly efficient and colorimetric sensor for the rapid detection of L-DOPA, utilizing manganese-doped graphitic carbon nitride (Mn-g-C 3 N 4 ) as a peroxidase-mimetic catalyst. The sensor employs 3,3′,5,5′-tetramethylbenzidine as a substrate and achieves detection within 20 s, with a linear response over the concentration ranges of 1–10 μM and 20–90 μM, and the detection limits were 0.049 μM and 0.51 μM, respectively. The method has good specificity for L-DOPA and has been successfully applied to human serum even in the presence of interference from dopamine and other amino acids. In addition, the sensor exhibits satisfactory performance in smartphone-based L-DOPA detection. The novel sensor platform offers a simple, cost-effective, and scalable solution for real-time L-DOPA monitoring, with significant potential for applications in both clinical and environmental contexts.
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