FOOD CHEMISTRY
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2020
·
IF 6.306
Manli Guo,Jingtian Chi,Chi Zhang,Minglin Wang,Hui Liang,Juying Hou,Shiyun Ai,Xiangyang Li
In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose. Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability. Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together. X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8. Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite. Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2020
·Vol. 192
·期 3
·935-951
·
IF 2.277
Guo Zongren,Long Liangkun,Ding Shaojun
L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase (BvAI) from Bacillus velezensis CICC 24777 was cloned and overexpressed in Escherichia coli BL21 (DE3) strain. The maximum activity of recombinant BvAI was observed at 45 °C and pH 8.0, in the presence of 1.0 mM Mn 2+ . Approximately 207.2 g/L L-ribulose was obtained from 300 g/L L-arabinose in 1.5 h by E. coli harboring BvAI. In addition, approximately 74.25 g/L L-ribose was produced from 300 g/L L-arabinose in 7 h by E. coli co-expressing BvAI and L-RI from Actinotalea fermentans ATCC 43279 (AfRI). This study provides a feasible approach for producing L-ribose from L-arabinose using a co-expression system harboring L-Al and L-RI.
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Materials
·
2020
·Vol. 13
·期 3
·507
·
IF 3.057
Hui Wang,Meiyin Wang,Jining Shang,Yuanhang Ren,Bin Yue,Heyong He
In this work various amount of phosphomolybdic acid (PMo) were immobilized on amine functionalized SBA-15 and used as heterogeneous catalysts in the epimerization of glucose in aqueous solution. 13.3PMo/NH2-SBA-15 exhibited the best catalytic performance with a glucose conversion of 34.8% and mannose selectivity of 85.6% within two hours at 120 °C. The activation energy of 80.1 ± 0.1 kJ·mol−1was lower than that of 96 kJ·mol−1over the homogeneous H3PMo12O40catalyst. The catalytic activities of 13.3PMo/NH2-SBA-15 for the transformation of some other aldoses including mannose, arabinose and xylose were also investigated.Keywords:aldose epimerization;phosphomolybdic acid;amino-silanization;SBA-15
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AMB Express
·
2019
·Vol. 9
·期 1
·1-11
·
IF 2.226
Guo Zongren,Long Liangkun,Ding Shaojun
d -Mannose and l -ribose are two important monosaccharides, which have attracted public attention recently because of their great application potentials in food, cosmetic and pharmaceutical industries. Sugar isomerases catalyze the sugar isomerization and therefore can be used as the biocatalysts for production of the high-value sugars from inexpensive sugars. l -arabinose isomerase catalyzes the conversion of l -arabinose to l -ribulose, while d -lyxose isomerase catalyzes l -ribulose and d -fructose to l -ribose and d -mannose, respectively. In this paper, a putative d -LI from Bacillus velezensis (BvLI) was identified, characterized and used to produce d -mannose and l -ribose from d -fructose and l -arabinose, respectively. The recombinant BvLI exhibited a maximum activity at 55 °C and pH 6.5, in the presence of 0.1 mM Co 2+ . Approximately 110.75 g/L d -mannose was obtained from 500 g/L d -fructose in 6 h by the recombinant BvLI, and approximately 105 g/L l -ribose was obtained from 500 g/L l -arabinose in 8 h by the successive biocatalysis of l -arabinose isomerase from Bacillus licheniformis (BlAI) and BvLI.
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RSC Advances
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2018
·Vol. 8
·期 30
·16574-16584
·
IF 2.936
Hong-Xun Wang,Yang Yi,Jie Sun,Olusola Lamikanra,Ting Min
Thirty-nine polysaccharides isolated from different parts of 13 lotus root varieties were characterized with fingerprint and chemometrics analyses to explore their similarity and diversity. The physicochemical features of lotus root polysaccharides (LRPs) were found to be the following: LRPs contained mainly polysaccharides (5.94 kDa) and polysaccharide-protein complexes (11.57 kDa and 5.30 kDa); their carbohydrates were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose approximately in the molar ratio of 0.19 : 0.14 : 0.08 : 0.17 : 6.49 : 1.00 : 0.16; and node LRPs possessed more binding proteins and uronic acids than both flesh and peel LRPs. Their fingerprints based on Fourier-transform infrared spectroscopy, pre-column derivatization high-performance liquid chromatography and high performance size-exclusion chromatography all exhibited relatively high similarities, contributing to the common figerprint models which could be utilized as references for the identification of LPRs. In addition, the fingerprint characteristics associated with the between-group variability of LRPs in the score plots derived from multivariate analytical models might indicate which variety or part of lotus root they were isolated from. Therefore, multi-fingerprinting techniques have the potential to be applied to the identification and quality control of LRPs.
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Xiaohui Zhang,Zhengyu Hu,Yuanqi Duan,Yuxin Jiang,Weiwei Xu,Pengcheng Yang,Wei Zhou,Jinfeng Sun,Gao Li
The purpose of this study was to optimize the extraction process of polysaccharides from the stems of Panax ginseng C. A. Meyer using different solvents (water, acid and alkali), and investigate their protective effects on PC12 cells against Amyloid-β 1–42 (Aβ 1–42 )-induced cell apoptosis. Three polysaccharides (W-GSP, Ac-GSP and Al-GSP) were prepared and optimized: liquid to material ratio 27:1 mL/g, extraction temperature 89°C, extraction time 2.9 h and 0.3 mol/L HCl/NaOH. The results of characterization indicated that the monosaccharides of W-GSP (703.80 kDa), Ac-GSP (641.38 kDa) and Al-GSP (457.04 kDa) all composed mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose with different molar ratios. Interestingly, W-GSP possessed a lower uronic acid, higher molecular weight, rougher surface and poorer thermal stability, whereby W-GSP exhibited a protective effect against Aβ 1–42 -induced apoptosis in PC12 cells by mediating the JNK signaling pathway, restoring the mitochondrial membrane potential and reducing intracellular ROS levels. This study should be provided the theoretical basis for the study and exploitation of polysaccharides from the stems of Panax ginseng C. A. Meyer.
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Frontiers in Sustainable Food Systems
·
2024
·
IF 3.7
Weiwei Xu,Wei Zhou,Jinfeng Sun,Weiwei Chen,Xuanye Wu,Tong Guan,Yilin Zhao,Pengcheng Yang,Zhengyu Hu,Gao Li
The purpose of this study was to obtain polysaccharides from Panax ginseng C. A. Meyer stems (PGSPs), agro-byproducts with development potential, and fully explore the potential value in P. ginseng stems. Two novel polysaccharides firstly from P. ginseng stems (PGSP-1 and PGSP-2) were obtained by water extraction and alcohol precipitation method and column chromatography, and then characterized by FT-IR, HPGPC, HPLC, SEM, TGA, GC–MS and NMR. The results demonstrated that PGSP-1 (Mw = 723.83 kDa) and PGSP-2 (Mw = 620.48 kDa) were characterized →4)-β-D-Galp-(1→, →6)-α-D-Glcp-(1 → and →2)-α-L-Rhap-(1 → as the skeleton, →4,6)-β-D-Galp-(1 → and →2,6)-α-D-Manp-(1 → as the cross junction, α-L-Araf-(1 → as the terminal unit, and PGSP-1 still contained →4)-β-D-Galp, while PGSP-2 contained →4)-β-D-Xylp-(1→, →3)-β-D-GlcpA-(1→, →4)-β-D-GalpA-(1 →) and α-D-Glcp-(1→, with different microstructures and thermal stability. And the results of hypoglycemic activity revealed that both PGSP-1 and PGSP-2 showed excellent inhibitory activity against α-glucosidase and α-amylase, in which PGSP-2 had the better performance. The inhibition kinetics result showed that PGSPs on α-glucosidase and α-amylase were non-competitive type and mixed type inhibition. This study provided a theoretical basis for making full use of and exploiting the economic value of agro-byproducts such as P. ginseng stems and offered a theoretical reference for the effective utilization of PGSP as a functional component to prevent and reduce T2DM.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Yuanqi Duan,Zhengyu Hu,Long Jin,Tieqiang Zong,Xiaohui Zhang,Yanan Liu,Pengcheng Yang,Jinfeng Sun,Wei Zhou,Gao Li
The degradation of Belamcanda chinensis (L.) DC. polysaccharides was carried out by five concentrations of trifluoroacetic acid (TFA) (1–5 mol/L), and their physicochemical properties, degradation kinetics and anticomplementary activity were investigated. The findings revealed a notable reduction in the molecular weight of BCP, from an initial value of 2.622 × 10 5 g/mol to a final value of 6.255 × 10 4 g/mol, and the water solubility index increased from 90.66 ± 0.42 % to 97.78 ± 0.43 %. The degraded polysaccharides of B. chinensis exhibited a comparable monosaccharide composition comprising Man, GalA, Glc, Gal, and Ara. As the concentration of TFA increased, the degradation rate constant increased from 1.468 × 10 −3 to 5.943 × 10 −3 , and the process followed the first-order degradation kinetic model (R 2 > 0.97) and the random fracture model (R 2 > 0.96). Furthermore, the five degraded polysaccharides still exhibit good thermal stability. In vitro experiments showed that DBCP-3 exhibited more potent anticomplementary activity than the original polysaccharides and positive drugs, which was strongly correlated with its Mw ( r = 0.6–0.8), inhibiting complement activation by blocking C2 and C4. These results indicated that TFA degradation has a positive effect on polysaccharides, of which DBCP-3 is expected to treat diseases involving hyperactivation of the complement system.
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-20
·
IF 3.8
Chang Shuaishuai,Lei Xuanhao,Xu Weijia,Guan Feng,Ge Jian,Nian Fuzhao
This study aimed to investigate the structural properties of tobacco polysaccharide (TP) and its mechanism of modulating hyperlipidemia in high-fat diet-induced mice. The structural properties of TP were characterized by FT-IR, 1HNMR, SEM, AFM and thermogravimetric analysis. And the regulatory mechanism of TP on lipid metabolism was investigated in hyperlipidemia mice. These results showed that TP had a high composition of reducing monosaccharide and the glycosidic bond type was α-glycosidic bond. The intervention by TP resulted in a significant reduction of body weight and improvement in lipid accumulation. And the modulation mechanism by which TP ameliorated the abnormalities of lipid metabolism was associated with the expression levels of lipid metabolism-related genes and serum exosomes miRNA-128-3p, as well as the modulation of structure and abundance of the gut microbiota in mice. In addition, TP treatment significantly increased the content of short-chain fatty acids (SCFAs) in mice feces. The results of molecular docking and dual-luciferase assay exhibited a good interaction between propionic acid and PPAR-α, and it was hypothesized that the interaction might further ameliorate the hyperlipidemia. Therefore, TP can regulate the expression levels of lipid metabolism-related genes through miRNAs from serum exosomes and SCFAs from gut microbiota.
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Journal of CO2 Utilization
·
2024
·
IF 7.2
Yiming Wang,Wensheng Wei,Yuxin Wang,Guangwen Xu,Jinggang Zhao,Lei Shi
In this study, a series of hydroxy-functionalized quaternary ammonium salt catalysts, i.e., NEt 3 (HE)Br, NEt 2 (HE) 2 Br, NEt 1 (HE) 3 Br, and N(HE) 4 Br, were successfully prepared by quantitatively grafting hydrogen-bond donors (HBDs) as electrophilic sites on quaternary ammonium salts and then used to catalyze the cycloaddition of propylene oxide (PO) and carbon dioxide (CO 2 ). The aim was to reveal the effects of different amounts of hydroxy-functionalized quaternary ammonium salts on their catalytic activities. The synergistic catalytic effect of NEt 3 (HE)Br and a biomass-based catalytic system (NEt 3 (HE)Br/Bio) on the cycloaddition reaction was systematically studied, and the influence mechanism of hydroxy-rich biomass on the cycloaddition reaction was elucidated. The promoting effects of the hydroxyl-group content, position and connection structure in different alcohol additives on the CO 2 cycloaddition reaction were further investigated and the results demonstrated that when the additive molecule contains an appropriate number of ortho-hydroxyl groups and these hydroxyl groups are connected to groups with strong electron-withdrawing ability and small steric hindrance, the cycloaddition efficiency between PO and CO₂ can be significantly enhanced. The reason is that these hydroxyl groups not only provide more hydrogen bonds, enhancing the intermolecular interactions, but also help to stabilize the transition state of the reaction. Notably, when 0.48 g of NEt 3 (HE)Br was mixed with 0.48 g of cellulose as HBDs to catalyze the cycloaddition reaction of CO 2 and PO, PO conversion reached 99.34 % within 2 h, exceeding that obtained with the catalytic reaction system without HBDs. In addition, the NEt 3 (HE)Br/cellulose catalytic system was demonstrated to be universal for catalyzing the cycloaddition reaction of different epoxides and CO 2 . This article provides theoretical guidance and new inspirations for the efficient utilization of hydroxy-containing biomass materials in CO 2 conversion.
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ChemistrySelect
·
2025
·Vol. 10
·期 25
·e00963
·
IF 2
Lihan Yue,Ming Zhao,Qiang Zhou,Liqiu Sun,Dan Wang,Jun Li,Zhichun Shi
Graphical In this study, three water-soluble polysaccharides were isolated from Isatidis Radix , which had never been reported before. IRPS-1 and IRPS-2 possess starch branch chains, while IRPS-3 has a stable triple helix structure. Compared to IRPS-1 and IRPS-2, the structure of IRPS-3 has two additional branches, namely →4)Arap(1→ and Ribf(1→. All three polysaccharides exhibited significant antioxidant activities in vitro, with IRPS-3 demonstrating the best activity among them. This study was focused on the objective of investigating the composition, structure, and physicochemical attributes of Isatidis Radix polysaccharides (IRP). Three distinct polysaccharide fractions, IRPS-1, IRPS-2, and IRPS-3, were separated and purified from the crude polysaccharides of Isatidis Radix utilizing DEAE-52 cellulose and Sephadex G-100 columns. A series of technical methods were used to analyze the structure of the three polysaccharides. The analysis indicated that these compounds were heteropolysaccharides, with average molecular weights of 1.17 × 10 5 Da, 1.21 × 10 5 Da, and 1.06 × 10 5 Da. The primary compositional of IRPS-1 and IRPS-2 were glucose and galactose, while IRPS-3 consisted primarily of galactose, glucose, and arabinose. The methylation analysis elucidated that IRPS-1, IRPS-2, and IRPS-3 possessed similar linear backbones but differed in their branches. Distinguished from the other two polysaccharides, IRPS-3 has two additional branches, namely →4)Arap(1→ and Ribf(1→. I 2 -KI experiment showed that IRPS-1 and IRPS-2 had starch branch chains. In addition, IRPS-3 exhibited a stable triple-helix configuration in aqueous milieu and possessed longer side chains and more branches. In vitro antioxidant experiments demonstrated that IRP exhibited good concentration-dependent antioxidant activity. Based on these findings, IRP can be regarded as a potential natural antioxidant.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Wei Zong,Zijing Liu,Haiyan Lan,Jianwei Yang,Yuxuan Xia,Zhongsheng Xu,Li Mai,Jie Wang,Yixi Bao
The polarization of tumor-associated macrophages (TAMs) from M2 to M1 phenotype is an effective strategy for tumor immunotherapy. In this study, a pectic polysaccharide (PEP-1) was isolated from Phyllanthus emblica fruits via hot water extraction, ethanol precipitation, and purified by anion exchange and gel permeation chromatography. PEP-1 was identified as a homogalacturonan (molecular weight, 156.8 kDa) via characterization analysis. The structural attributes of PEP-1 were elucidated through GC–MS and NMR analyses, confirming that its repeating sugar units consist of GalA connected by α-1,4-glycosidic linkage. Functional assays demonstrated that PEP-1, at concentrations of 50, 100, and 200 μg/mL could strongly induce the transformation of M2 macrophages into M1 phenotype in vitro, and the conditioned medium (CM) of M2 pretreated with PEP-1 significantly promoted apoptosis of Hepa1-6 cells. At the cellular level, PEP-1 shifted tumor-promoting M2 macrophages to a tumor-inhibiting M1 phenotype by increasing the phosphorylation of NF-κB and MAPK. Additionally, PEP-1 (200 mg/kg) was capable of reaching the tumor microenvironment (TME), directly binding to TAMs, promoting their polarization towards M1 phenotype, and significantly inhibiting the tumor growth in Hepa1-6 tumor-bearing mice. These findings reveal the structural characteristics of PEP-1 and its potential to treat hepatocellular carcinoma immunotherapy via regulating TAMs.
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